Croatian Digital Thesis Repository
Not a member yet
259189 research outputs found
Sort by
Technological project for the production of solid wood furniture
No full textU ovom diplomskom radu analiziran je postojeći pogon primarne prerade drva (pilana), te je izrađen tehnološki projekt za drvno
industrijski pogon proizvodnje namještaja od cjelovitog drva. Na temelju odabranog asortimana proizvoda predložena su; konstrukcijska rješenja istih, tehnološki procesi, utrošak osnovnog i pomoćnog materijala, izbor strojeva i njihove produktivnosti, Izrađen je proračun kapaciteta i cijena koštanja za odabrani proizvod.In this master's thesis the existing primary wood processing plant (sawmill) was analyzed, and a technological project was created for a wood industrial facility for the production of furniture made of solid wood. Based on the selected range of products, the following are proposed: structural solutions of the same, technological processes, consumption of basic and auxiliary materials, choice of machines and their productivity. The capacity budget and the cost price for the selected product were prepared
Wastewater and environment
No full textOnečišćenje vodenih sustava otpadnim vodama je istaknuti problem koji izaziva veliku zabrinutost. S porastom svjetske populacije raste i količina otpadnih voda koju je potrebno učinkovito obraditi. Značajne količine otpadnih voda se ispuštaju u vodeni okoliš, stvarajući ozbiljne prijetnje za ekosustave i dobrobit ljudskog zdravlja. Kako bi se spriječilo daljnje onečišćenje, iznimno je važno razumijevanje sastava otpadnih voda čime se omogućuje njihova ciljana obrada i povećava učinkovitost samog procesa. U okviru završnog rada dan je pregled izvora i vrsta otpadnih voda, postupci obrade otpadnih voda s naglaskom na industrijske otpadne vode iz farmaceutske proizvodnje.The pollution of water systems by wastewater is a major problem that gives cause for great concern. As the world's population grows, so does the amount of wastewater that needs to be treated effectively. Significant amounts of wastewater are being discharged into water bodies, posing a serious threat to ecosystems and human health. To prevent further pollution, it is extremely important to understand the composition of wastewater, treat it in a targeted manner, and increase the efficiency of the process. The thesis provided an overview of the sources and types of wastewater, and wastewater treatment processes with a focus on industrial wastewater from pharmaceutical production
Synthesis of novel biosurfactants
No full textEnzimi i biokataliza su vrlo značajni u suvremenoj kemiji jer omogućuju selektivne i ekološki prihvatljive reakcije. Enzimi, kao biokatalizatori, osiguravaju učinkovitiju i održiviju sintezu brojnih kemijskih spojeva, uključujući biosurfaktante. Biosurfaktanti su amfifilni spojevi koji su površinski aktivni, a u usporedbi s kemijski sintetiziranim surfaktantima, posjeduju veću biorazgradivost, manju toksičnost, specifičnost i sposobnost djelovanja u ekstremnim uvjetima. Zbog svojih jedinstvenih svojstava i ekoloških prednosti, biosurfaktanti pronalaze široku primjenu u različitim industrijama te predstavljaju alternativu konvencionalnim surfaktantima. Ovaj diplomski rad istražuje sintezu novih biosurfaktanata – soforolipida. Cilj istraživanja bio je usavršiti postupak enzimske modifikacije soforolipida u različitim vrstama reaktora te zaključiti koji reaktor je najprikladniji za provođenje reakcije. U eksperimentalnom dijelu provedene su reakcije u tri različita tipa reaktora. Ispitani su šaržni reaktor, reaktor s dotokom supstrata i enzimski membranski reaktor, pri čemu su korištene HPLC-UV/VIS i RI analize za praćenje koncentracije supstrata i produkata. Rezultati su pokazali da je šaržni reaktor najprikladniji za enzimsku modifikaciju soforolipida, omogućujući najbolje uvjete za postizanje najveće konverzije supstrata i iskorištenja.Enzymes and biocatalysis are highly significant in modern chemistry as they enable selective and environmentally friendly reactions. Enzymes, as biocatalysts, provide a more efficient and sustainable synthesis of numerous chemical compounds, including biosurfactants. Biosurfactants are amphiphilic compounds with surface-active properties, and compared to chemically synthesized surfactants, they exhibit higher biodegradability, lower toxicity, specificity, and the ability to function under extreme conditions. Due to their unique properties and environmental benefits, biosurfactants find broad applications in various industries and represent an alternative to conventional surfactants. This master’s thesis explores the synthesis of new biosurfactants – sophorolipids. The aim of the research was to optimize the process of enzymatic modification of sophorolipids in different types of reactors and to determine which reactor is the most suitable for carrying out the reaction. In the experimental section, reactions were conducted in three different types of reactors. A batch reactor, a fed-batch reactor, and an enzymatic membrane reactor were examined, using HPLC-UV/VIS and RI analyses to monitor the concentration of substrates and products. The results showed that the batch reactor is the most suitable for the enzymatic modification of sophorolipids, providing the best conditions for achieving the highest substrate conversion and product yield
Contribution of different estrogen receptor alpha (ER) domains in ER-driven transcriptomic profile of mammary epithelial cells and characterization of the ER-PR proximity in ER+ breast cancer
No full textAim of the study: 70% of breast cancers (BC) are estrogen receptor alphapositive (ER+), and typically treated by targeting ER signaling. Previous research showed that membrane ER, as well as activation function 1 (AF-1) and activation function 2 (AF-2) domains are involved in the mammary gland and BC development. We aimed to characterize the contribution of each ER domain in ER regulated transcriptional profile in mammary epithelial cells (MEC). First, we researched whether ER-dependent gene
expression is domain-specific. We tested the hypothesis that pathways relevant for BC are ER domain-dependent, and aimed to characterize whether those pathways are regulated by the cooperation of all domains or by an individual domain. We focused on the dependences of the expression of ER target genes, such as Areg, Greb1, and Pgr on different ER domains. Finally, we aimed to characterize the levels of interactions between PR and ER in cancer cell lines MCF7 and T47D. Material and methods: In this
thesis, RNAseq data of GFP+ MECs from C57Bl6 mice lacking complete ER, membrane ER, AF-1 or AF-2 ER domains were analyzed in R software. MCF7 and T47D cell lines were treated with 100 nM progesterone (P4) to establish the conditions suitable for ER-PR interactions. The expression of Pgr was measured by qPCR and ER-PR interactions were analyzed by proximity ligation assay. Results and conclusions: Each ER domain strongly contributes to ER-regulated gene expression in MECs. To regulate pathways involved in carcinogenesis, cell proliferation, and immune response, ER domains act synergically, but also, in some pathways, an individual domain plays a regulatory role of the whole receptor. ER-PR direct interactions occur
in nuclei, but also in non-nuclear compartments in MCF7 and T47D cells with an increased number of interactions upon P4 treatment
Stanično-specifična uloga optineurina u TLR4 ili ER stresom posredovanoj aktivaciji NF-κB puta
No full textAmyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease, which leads to motor neuron death in the brain and the spinal cord. In 2010 the first optineurin mutations were found in the ALS patients, and the initial biochemical analyses in cell lines suggested that optineurin truncation or mutation in the ubiquitin binding region result in exaggerated nuclear factor κB (NF-κB) activation. Such excessive NF-κB activation and subsequent inflammation were reported to result in neuronal cell death. However, several follow-up studies in primary innate immune cells from optineurin deficiency or insufficiency models could not reproduce these findings. To further test this issue, we decided to induce NF-κB activation with paclitaxel [Toll-like receptor 4 (TLR4) agonist] and tunicamycin [endoplasmic reticulum (ER) stress inducer] to observe if optineurin will exert its role as a negative regulator. These stimuli could mimic some of the stresses that central nervous system (CNS) cells are exposed to during the neurodegenerative process. Microglial (BV2) and neuronal (N2A) optineurin wild-type (WT) and knock-out (KO) cells were used in this research. Optineurin KO BV2 cells exhibited a diminished p65 phosphorylation and TNF-α secretion upon paclitaxel treatment but stronger inhibitor of κB-α (IκB-α) degradation, while N2A KO cells exhibited slightly higher NF-κB activation as detected by p65 phosphorylation. Tunicamycin treated optineurin KO BV2 cells also revealed slightly higher IκB-α degradation and diminished p65 phosphorylation, but TNF-α secretion was not different. Notably, optineurin KO N2A cells also exhibited a slight tendency of stronger NF-κB activation as evidenced by p65 phosphorylation. Results obtained from N2A cells are in correlation with previous studies done on neuronal cell lines, while the ones obtained in BV2 cells differ from results done on primary microglia. Taken together, we propose that optineurin has cell-specific effects, acting as a negative regulator of NF-κB activation in BV2 and N2A cells in response to paclitaxel. It must be noted that these results are obtained in cell lines so they should be used as a preliminary information for subsequent studies on primary cells or in vivo models.Amiotrofična lateralna skleroza (ALS) je fatalna neurodegenerativna bolest koja dovodi do smrti motoričkih neurona u mozgu i leđnoj moždini. Prve mutacije optineurina pronađene su u pacijenata oboljelih od ALS-a 2010. godine. Prvotne biokemijske analize u staničnim linijama pokazale su da trunkacija ili mutacija ubikvitin vežuće regije optineurina dovodi do pojačane aktivacije puta nuklearnog čimbenika κB (NF-κB). Pretjerana NF-κB aktivacija te posljedična upala mogu dovesti do neuralne stanične smrti. Međutim, nekoliko studija napravljenih na primarnim stanicama urođene imunosti izoliranih iz modela deficijencije ili insuficijencije optineurina nisu mogle reproducirati rezultate dobivene na staničnim linijama. U svrhu ispitivanja tih kontradikcija, odlučili smo inducirati NF-κB aktivaciju s paklitakselom [agoinst receptora sličnom Toll-u (TLR4)] i tunikamicinom [izaziva stres endoplazmatskog retikuluma (ER)] kako bismo ispitali hoće li optineurin djelovati kao negativan regulator. Navedeni stimulusi mogu imitirati stresore kojem su stanice središnjeg živčanog sustava izložene prilikom procesa neurodegeneracije. U ovom istraživanju koristili smo migroglijalne (BV2) i neuronalne (N2A) stanične linije divljeg tipa (eng. wild-type, WT) i optineurin deficijentne (eng. knock-out, KO). BV2 KO stanice su pokazale smanjenu fosforilaciju p65 aktivaciju i sekreciju TNF-α, no jaču razgradnju inhibitora κB-α (IκB-α) nakon tretmana s paklitakselom. U N2A KO stanicama tretman paklitakselom rezultirao je blago pojačanom NF-κB aktivacijom što je zabilježeno fosforilacijom p65. BV2 KO stanice tretirane s tunikamicinom su pokazale jaču razgradnju IκB-α i smanjenu fosforilaciju p65, no razlika u izlučivanju TNF-α nije zabilježena. N2A KO stanice pokazale su tendenciju blago pojačane NF-κB aktivacije nakon tretmana tunikamicinom što je praćeno fosforilacijom p65. Rezultati dobiveni na N2A stanicama se slažu s prethodnim studijama provedenim na neuronalnim staničnim linijama. Međutim, rezultati dobiveni na BV2 stanicama se ne slažu s onim dobivenim na primarnoj mikrogliji. Uzimajući sve rezultate u obzir, predlažemo da optineurin ima stanično-specifični, te da ima potencijalnu ulogu kao negativan regulator NF-κB aktivacije u BV2 i N2A stanicama. Moralo bi se uzeti u obzir kako su ovi rezultati dobiveni iz staničnih linija te bi se pritom trebali koristiti kao preliminarna informacija za buduće studije koje bi se provele na primarnim stanicama ili u in vivo modelima
Analysis of NK cells in CD16 deficient animals
No full textPrirodnoubilačke ili stanice NK (engl. natural killer cells, NK cells) važna su sastavnica urođenog imunološkog sustava zbog svoje sposobnosti brzog prepoznavanja i eliminacije tumorskih i virusom zaraženih stanica. Njihova aktivacija ovisi o ravnoteži signala primljenih preko aktivacijskih i inhibicijskih receptora. Jedan od aktivacijskih receptora izraženih na stanicama NK je i CD16 receptor, poznat po svojoj ulozi u staničnoj citotoksičnosti posredovanoj protutijelima (engl. antibody-dependent cellular cytotoxicity, ADCC). Međutim, uloga ovog receptora u drugim aspektima biologije stanica NK još uvijek nije dovoljno istražena. Stoga je naš glavni cilj bio ispitati utjecaj nedostatka izražaja CD16 receptora na stanice NK u različitim organima. Koristili smo genetski modificirane miševe kojima nedostaje izražaj CD16 receptora i usporedili ih s miševima divljeg soja. Proveli smo fenotipsku i funkcionalnu analizu stanica NK. Uočili smo da nedostatak izražaja CD16 receptora dovodi do promjene u sazrijevanju stanica NK, kao i do promjena u njihovom postotku i broju u koštanoj srži. Nedostatak izražaja ovog receptora nije doveo do promjene u izražaju većine analiziranih aktivacijskih i inhibicijskih receptora, izuzev NCR1 receptora čiji je izražaj smanjen kod CD16 deficitnih životinja. Također, CD16 deficitne stanice NK nisu pokazale razliku u sposobnosti produkcije citokina u odnosu na kontrolnu grupu. Rezultati ovoga rada ukazuju na dosad nepoznatu ulogu CD16 receptora u sazrijevanju stanica NK iako su potrebna dodatna istraživanja kako bi se ta uloga do kraja istražila.Natural killer (NK) cells are an important component of the innate immune system due to their ability to rapidly recognize and eliminate tumor cells and virus-infected cells. Their activation depends on a balance of signals received through activating and inhibitory receptors. One key activating receptor expressed on NK cells is the CD16 receptor, known for its role in antibody-dependent cellular cytotoxicity (ADCC). However, the role of this receptor in other aspects of NK cell biology remains largely unexplored. The main aim of this study was to investigate the impact of CD16 receptor deficiency on NK cells in different organs. We used genetically modified mice lacking CD16 expression and compared them to wild-type mice. We performed phenotypic and functional analyses of NK cells. We observed that the absence of CD16 expression leads to changes in NK cell maturation, as well as changes in their percentage and number in the bone marrow. The absence of this receptor did not result in changes in the expression of most analysed activating and inhibitory receptors, except for the NCR1 receptor, which showed reduced expression in CD16-deficient animals. Additionally, CD16-deficient NK cells did not show differences in cytokine production capacity compared to the control group. These findings indicate a previously unknown role of the CD16 receptor in NK cell maturation, although further studies are needed to fully elucidate this role
Development of an information system to support auditor decision-making in the supervision of the Quality Management System
No full textU ovom radu, povezuje se koncept upravljanja kvalitetom prema zahtjevima norme ISO 9001:2015 s pojmom umjetne inteligencije primjenom velikih jezičnih modela pri ocjeni usklađenosti sustava upravljanja kvalitetom sa zahtjevima norme. Nakon kratkog pregleda osnovnih pojmovima sustava upravljanja kvalitetom, analizirani su zahtjevi norme ISO 9001:2015 relevantni za proces internog audita i uspostavljeni proces internog audita u organizaciji Klimaoprema d.o.o. te su istražene mogućnosti primjene velikih jezičnih modela pri obradi prikupljenih podataka tijekom provedbe internog audita. Središnji dio rada obuhvaća razvoj informacijskog sustava za potporu odlučivanju auditora pri nadzoru sustava upravljanja kvalitetom koji je razvijen sukladno prikazanom procesu rada i podatkovnoj strukturi. Opis razvoja sustava uključuje prikaz korištenih tehnologija, arhitekture sustava i strukture koda te postavljanje sustava i integraciju umjetne inteligencije primjenom inženjeringa upita. Na posljetku, provedena je evaluacija razvijenog informacijskog sustava i dani su prijedlozi za daljnje unaprjeđenje sustava.In this paper, the concept of quality management according to the requirements of the ISO 9001:2015 standard is linked to artificial intelligence through the application of large language models (LLM) in assesing the compliance of a Quality Management Aystem with the standard’s requirements. After a brief overview of fundamental quality management concepts, the paper analyses ISO 9001:2015 requirements relevant to the internal audit process and examines the established internal audit procedure in the company Klimaoprema d.o.o., exploring the potential of large language models in processing the data collected during internal audits. The central part of the paper includes the development of an information system to support auditor decision-making in the supervision of the Quality Management System, developed in accordance with the defined workflow and data structure. The system development description includes an overview of the technologies used, system architecture, code structure, system deployment, and integration of artificial intelligence using prompt engineering. Finally, the developed information system is evaluated, and recommendations for future system improvements are provided
Effect of optineurin deficiency on IFN-β-induced autophagy
No full textUčinci različitih neurodegenerativnih bolesti na živčane stanice međusobno se bitno razlikuju, međutim nakupljanje unutarstaničnih, toksičnih agregata krivo smotanih proteina i posljedično gubitak funkcije stanica, zajednička su obilježja svih neurodegenerativnih bolesti. Osnovni razlog prisutnosti agregata nije poznat u velikoj većini slučajeva, ali dio mutacija u genima povezanim s neurodegenerativnim bolestima veže se uz autofagiju. Autofagija je evolucijski očuvani proces kojim se putem lizosoma razgrađuje citoplazmatski sadržaj poput proteinskih agregata, patogena i oštećenih organela. Pritom adaptori autofagije prepoznaju označeni sadržaj za razgradnju i dostavljaju ga do vezikula s dvostrukom membranom koje se zovu autofagosomi. Autofagosomi se spajaju s lizosomima što dovodi do stvaranja autolizosoma gdje se sadržaj razgrađuje. U adaptore autofagije ubrajaju se i dva proteina čije su mutacije pronađene u bolesnika s neurodegenerativnom bolešću amiotrofičnom lateralnom sklerozom (ALS), optineurin (OPTN) i sekvestosom 1 (SQSTM1 ili p62). Za OPTN je poznato da osim kao adaptor autofagije, sudjeluje u još dva koraka autofagije: inicijaciji autofagije i sazrijevanju autofagosoma. Stoga smo u ovom radu htjeli ispitati utjecaj OPTN-a na autofagiju u staničnoj liniji neuroblastoma N2a, koristeći N2a stanice u kojima je OPTN uklonjen CRISPR-Cas9 metodom. Analizirali smo bazalnu autofagiju i autofagiju induciranu s interferonom (IFN)-β, citokinom za koji je nedavno pokazano da regulira autofagiju u neuronima. Koristili smo standardne testove za praćenje autofagije poput mjerenja količine markera autofagosoma LC3-II (engl. microtubule associated protein light chain 3), adaptora p62, i OPTN-a Western blot metodom, te brojanje LC3 pozitivnih vezikula imunofluorescencijom ili fluorescentno označenim LC3 konstruktom. Iz naših rezultata zaključujemo da u OPTN-deficijentnim stanicama postoji djelomični blok autofagije u bazalnim uvjetima kojeg vidimo kao povećani broj i veličinu autofagosoma te povećano nakupljanje LC3-II. IFN-β je u N2a stanicama inducirao autofagiju, ali nismo uočili nakupljanje OPTN i p62 što može značiti da nisu adaptori autofagije potaknute s IFN-β te da djeluju na autofagiju u nekom drugom koraku. Nadalje, moguće je i da su razgrađeni prije naše analize te da pokuse treba modificirati. Zbog djelomičnog bloka u autofagiji pri nedostatku OPTN-a, moguće je da iz tog razloga dolazi do nakupljanja proteinskih agregata u ALS-u. U budućim pokusima važno je stoga dokazati da opaženi poremećaj autofagije dovodi do agregacije proteina.The effects of different neurodegenerative diseases on neurons substantially differ, but the accumulation of intracellular toxic aggregates of misfolded proteins and the consequent loss of cell function are common features of all neurodegenerative diseases. The underlying reason for the presence of aggregates is not known in the vast majority of cases, but some genes mutated in neurodegenerative diseases are linked to autophagy. Autophagy is an evolutionarily conserved process by which noxious cytoplasmic contents such as protein aggregates, pathogens, and damaged organelles are broken down in lysosomes. Autophagy adaptors recognize the cargo labeled for degradation and deliver it to double-membrane vesicles called autophagosomes. Autophagosomes fuse with lysosomes leading to the formation of autolysosomes where the cargo is degraded. Autophagy adapters also include two proteins whose mutations have been found in patients with the neurodegenerative disease amyotrophic lateral sclerosis (ALS), optineurin (OPTN) and sequestosome 1 (SQSTM1 or p62). In addition to its adaptor role, OPTN has also been described to participate in two other autophagy steps: autophagy initiation and autophagosome maturation. Therefore, in this paper, we examined the effect of OPTN on autophagy in the N2a neuroblastoma cell line, using N2a cells in which OPTN was removed by the CRISPR-Cas9 approach. We analyzed basal autophagy and autophagy induced by interferon (IFN)-, a cytokine recently shown to regulate autophagy in neurons. We used standard tests to monitor autophagy such as measuring the amount of LC3-II (microtubule associated protein light chain 3), p62 adaptor, and OPTN by Western blotting, and counting LC3 positive vesicles by immunofluorescence or via a fluorescently labeled LC3 construct. We observed a partial block of basal autophagy in the OPTN-deficient cells manifested as an increased number and size of autophagosomes and increased accumulation of LC3-II. IFN-β induced autophagy in N2a cells, but we did not observe the accumulation of OPTN and p62 which may mean that they do not serve as autophagy adaptors upon IFN-β-treatment or that they act on autophagy in some other step(s). Furthermore, it is possible that they were degraded before our analysis and that our experimental approach needs to be modified. Due to the partial block in autophagy in the absence of OPTN, it is possible that this leads to an accumulation of protein aggregates in ALS. In future experiments, it is therefore important to test if this observed autophagy dysregulation in OPTN-deficient cells leads to protein aggregation
Application of De Novo programming for decision-making in production
No full textOsnovni zadatak proizvodnih poduzeća je učinkovito koristiti dostupne resurse kako bi proizvela proizvode u željenoj količini, kvaliteti, unutar zadanog vremena i s minimalnim troškovima. Ovaj rad istražuje primjenu kvantitativne metode De Novo programiranja za optimizaciju proizvodnje i donošenje odluka u proizvodnim sustavima. Prvo se daje pregled kvantitativnih metoda i sistematizacija programske podrške za optimizaciju. Zatim se u radu opisuje proizvodna funkcija poduzeća, uključujući proizvodni program i proces proizvodnje. U sklopu rada je potom primijenjena metoda De Novo programiranja kako bi se matematički modelirao konkretni problem u proizvodnji i našlo optimalno rješenje korištenjem programskog jezika Python. Na temelju analize dobivenih rezultata, dana je preporuka za donošenje odluka koju poduzeće može koristiti za poboljšanje svojih proizvodnih procesa.The primary task of manufacturing companies is to efficiently utilize available resources to produce products in the desired quantity and quality, within the given time frame, and at minimal costs. This thesis explores the application of the quantitative De Novo programming method for production optimization and decision-making in manufacturing systems. The paper first provides an overview of quantitative methods and a systematization of software tools for optimization. It then describes the production function of the company, including the production program and manufacturing process. Then, De Novo programming is applied to mathematically model a specific problem in production and find the optimal solution using the Python programming language. Based on the analysis of the obtained results, a decision-making recommendation is provided that the company can use to improve its production processes
Izrada prediktivnog modela procesa presvlačenja elektroda u proizvodnji baterijskih ćelija
No full textIn this master's thesis, an in-depth overview of battery manufacturing, as well as the theory behind basic machine learning algorithms, was provided, with specific focus on the anode coating process. The data pipeline of BMW's Battery Cell Competence Center (BCCC) was also described in detail, particularly emphasizing the coating process. The primary goal of this work was to develop a machine learning model to predict pump speed, thereby optimizing the production of battery prototypes at the BCCC. To this end, data preparation was conducted to create an interpretable and usable dataset. The prediction model was divided into two parts: the Wet Loading Model and the Pump Speed Model. Each part was separately assessed, trained, and tested. Finally, the thesis introduced a data quality check script and the concept of DMC tracking.U ovom diplomskom radu pružen je detaljan pregled proizvodnje baterija, kao i teorijska osnova osnovnih algoritama strojnog učenja, s posebnim naglaskom na proces nanošenja premaza na anodu. Također je detaljno opisan podatkovni tijek unutar BMW-ovog Battery Cell Competence Center (BCCC), s posebnim fokusom na proces premaza. Primarni cilj ovog rada bio je razviti model strojnog učenja za predviđanje brzine pumpe, čime bi se optimizirala proizvodnja prototipa baterija u BCCC-u. U tu svrhu provedena je priprema podataka kako bi se stvorio interpretabilan i upotrebljiv skup podataka. Prediktivni model podijeljen je u dva dijela: model mokrog opterećenja i model brzine pumpe. Svaki dio je zasebno procijenjen, treniran i testiran. Na kraju, rad uvodi skriptu za provjeru kvalitete podataka i koncept DMC praćenja