Korea Research Institute of Bioscience and Biotechnology
KRIBB Open Access RepositoryNot a member yet
15834 research outputs found
Sort by
Soybean cultivar ‘Hipro’ for tofu and soymilk with high seed protein content and pod shattering resistance
No full textA yellow soybean cultivar called ‘Hipro’ was developed using the single seed descent (SSD) method by crossing ‘Saedanbaek’ (SS92414 ×MD87L) and ‘Daepung’ (Baekwon × Sinpaldal2). A preliminary yield trial (PYT), advanced yield trial (AYT), and regional yield trial (RYT) were conducted in three regions. ‘Hipro’ has a determinate growth habit, white flowers, and a spherical seed shape. In the regional yield trial, t he f lowering a nd m atu rity d ates o f ‘Hipro’ were A ugu st 4 a nd O ctober 17, respectively, a nd t he 1 00-seed weight w as 22.2 g, which was lower than that of Daewon (26 g). Additionally, ‘Hipro’ showed resistance to lodging, soybean mosaic virus (SMV), bacterial blight, and pod shattering. The seed protein content of ‘Hipro’, which was of particular interest in this study, was 53.9%, which was higher than that of ‘Daepung’ (41.5%), and the total amino acid content without tryptophan was 43.6%, 19% higher than that of ‘Daepung’. The mean yield of ‘Hipro’ in RYT was 2.23 ton/ha, which was 91.3% of the yield of ‘Daewon’. However, ‘Hipro’ showed an 11% higher yield than Daewon in Paju. As a result of the tofu characteristic test, 'Hipro' is suitable for making soy milk and tofu due to its high solid content (12.67 Brix) and protein content (13.68%) and high yield (325%) of tofu. Overall, ‘Hipro’ had high protein content, excellent processing quality for tofu and soy milk, and strong resistance to disease and pod shattering that will greatly contribute to the consumption of soybeans in Korea. (Registration No. 7735).
Arenibacter arenosicollis sp. nov., isolated from a sand dune
No full textA Gram-stain-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterial strain, designated BSSL-BM3T, was isolated from sand collected from a dune near the Yellow Sea, Republic of Korea, and subjected to a polyphasic taxonomic study. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain BSSL-BM3T fell within the clade comprising the type strains of Arenibacter species. Strain BSSL-BM3T exhibited 16S rRNA gene sequence similarity values of 98.0？99.0？% to the type strains of Arenibacter catalasegens , Arenibacter hampyeongensis , Arenibacter echinorum , Arenibacter palladensis and Arenibacter troitsensis and of 94.2？96.7？% to the type strains of the other Arenibacter species. The averagenucleotide identity and digitalDNA？DNA hybridization values between strain BSSL-BM3T and the type strains of A. catalasegens , A. hampyeongensis , A. echinorum , A. palladensis and A. troitsensis were 82.2？88.8？%？and 25.0？36.5？%, respectively. The DNA G+C content of strain BSSL-BM3T from genomic sequence data was 38.75？mol%. Strain BSSL-BM3T contained MK-6 as the predominant menaquinone and iso-C17？:？0 3-OH, summed feature 3 (C16？:？1 ？ω7c and/or C16？:？1 ？ω6c) and iso-C15？:？1 G as the major fatty acids. The major polar lipids of strain BSSL-BM3T were phosphatidylethanolamine and two unidentified lipids. Distinguishing phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain BSSL-BM3T is separated from recognized Arenibacter species. On the basis of the data presented here, strain BSSL-BM3T is considered to represent a novel species of the genus Arenibacter , for which the name Arenibacter arenosicollis sp. nov. is proposed. The type strain is BSSL-BM3T (=KACC 21632T=NBRC 114502T).
Study for drug repositioning and target identification ofL-DOPA-induced dyskinesia in Parkinson’s disease
No full text파킨슨병에서 레보도파 유도 이상운동증 치료 약물 및 신규 타겟 후보도출OGM476201
Stepwise treatment of undiluted raw piggery wastewater, using three microalgal species adapted to high ammonia
No full textA high ammonia concentration and chemical oxygen demand (COD) in piggery wastewater force it to be diluted before conventional microalgal treatment to reduce ammonia toxicity. Incomplete treatment of ammonia and COD in piggery wastewater may cause eutrophication, resulting in algal blooms. This study tried to treat raw piggery wastewater without dilution, using three strains of microalgae (Chlorella sorokiniana, Coelastrella sp. and Acutodesmus nygaardii) that outcompeted other algae under heterotrophic, mixotrophic, and autotrophic conditions, respectively, through adaptive evolution at high ammonia concentration. The three stepwise processes were designed to remove (1) small particles, COD, and phosphorus in the 1st heterotrophic C. sorokiniana cultivation, (2) ammonia and COD in the 2nd mixotrophic Coelastrella sp. cultivation, and (3) the remaining ammonia in the 3rd photoautotrophic A. nygaardii cultivation. To enhance ammonia uptake rate, each algal species were inoculated after 2-day nitrogen starvation. When the N-starved three species were inoculated at each step sequentially at 7 g/L for 2 days, the final phosphorus, COD, and ammonia removal efficiencies were 100% (16.4？0 mg/L), 92% (6820？545 mg/L), 90% (850？81 mg/L) and turbidity (99%) after total 6 days.
Profiling genome-wide specificity of CRISPR-Cas9 using Digenome-seq
No full textDigenome-seq is a highly sensitive method for analyzing the genome-wide specificity of CRISPR-Cas9 nuclease activity. In this procedure, genomic DNA is first subjected to digestion by CRISPR-Cas9 in vitro and then to whole genome sequencing, which results in unusual patterns of straight alignments at on-target and potential off-target sites. Analysis of these data with the Digenome-seq computer program allows for identification of the in vitro cleavage sites associated with the straight alignments. Here, we present a detailed Digenome-seq protocol for genome-wide profiling of in vitro CRISPR-Cas9 nuclease cleavage sites.
Akt and calcium-permeable AMPA receptor are involved in the effect of pinoresinol on amyloid β-induced synaptic plasticity and memory deficits
No full textAlzheimer’s disease (AD) is one of the most prevalent neurodegenerative disorders characterized by memory deficits. Although no drug has given promising results, synaptic dysfunction-modulating agents might be considered potential candidates for alleviating this disorder. Pinoresinol, a lignan found in Forsythia suspensa, is a memory-enhancing agent with excitatory synaptic activation. In the present study, we tested whether pinoresinol reduces learning and memory and excitatory synaptic deficits in an amyloid β (Aβ)-induced AD-like mouse model. Pinoresinol enhanced hippocampal long-term potentiation (LTP) through calcium-permeable AMPA receptor, which was mediated by Akt activation. Moreover, pinoresinol ameliorated LTP deficits in amyloid β (Aβ)-treated hippocampal slices via Akt signaling. Oral administration of pinoresinol ameliorated Aβ-induced memory deficits without sensory dysfunction. Moreover, AD-like pathology, including neuroinflammation and synaptic deficit, were ameliorated by pinoresinol administration. Collectively, pinoresinol may be a good candidate for AD therapy by modulating synaptic functions.
Diversity and antimicrobial resistance in the Streptococcus bovis/Streptococcus equinus complex (SBSEC) isolated from Korean domestic ruminants
No full textS. bovis/S. equinus complex (SBSEC) includes lactic acid-producing bacteria considered as the causative agent associated with acute rumen lactic acidosis in intensive ruminants. Considering the limited information on the detailed characteristics and diversity of SBSEC in Korea and the emergence of antimicrobial resistance (AMR), we investigated the diversity of SBSEC from domestic ruminants and verified the presence of antimicrobial resistance genes (ARGs) against several antimicrobials with their phenotypic resistance. Among 51 SBSEC isolates collected, two SBSEC members (S. equinus and S. lutetiensis) were identified; sodA-based phylogenetic analyses and comparisons of overall genome relatedness revealed potential plasticity and diversity. The AMR rates of these SBSEC against erythromycin, clindamycin, and tetracycline were relatively lower than those of other SBSEC isolates of a clinical origin. An investigation of the ARGs against those antimicrobials indicated that tetracycline resistance of SBSECs generally correlated with the presence of tet(M)-possessing Tn916-like transposon. However, no correlation between the presence of ARGs and phenotypic resistance to erythromycin and clindamycin was observed. Although a limited number of animals and their SBSEC isolates were examined, this study provides insights into the potential intraspecies biodiversity of ruminant-origin SBSEC and the current status on antimicrobial resistance of the bacteria in the Korean livestock industry.
Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria
No full textLactobacillus reuteri AN417 is a newly characterized probiotic strain. The activity of AN417 against oral pathogenic bacteria is unknown. We investigated the antibacterial activity of cell-free L. reuteri AN417 culture supernatant (LRS) against three oral pathogens: Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans. P. gingivalis and F. nucleatum have been implicated in periodontal disease, whereas S. mutans causes dental caries. Exposing these oral pathogenic bacteria to LRS significantly reduced their growth rates, intracellular ATP levels, cell viability, and time-to-kill. The minimal inhibitory volume of LRS was 10% (v/v) against P. gingivalis, 20% (v/v) for F. nucleatum, and 30% (v/v) for S. mutans. LRS significantly reduced the integrity of biofilms and significantly suppressed the expression of various genes involved in P. gingivalis biofilm formation. The L. reuteri AN417 genome lacked genes encoding reuterin, reuteran, and reutericyclin, which are major antibacterial compounds produced in L. reuteri strains. LRS treated with lipase and α-amylase displayed decreased antibacterial activity against oral pathogens. These data suggest that the antibacterial substances in LRS are carbohydrates and/or fatty acid metabolites. Our results demonstrate that LRS has antimicrobial activity against dental pathogenic bacteria, highlighting its potential utility for the prevention and treatment of P. gingivalis periodontal disease.
O-GlcNAcylation ameliorates the pathological manifestations of Alzheimer's disease by inhibiting necroptosis
No full textO-GlcNAcylation (O-linked β-N-acetylglucosaminylation) is notably decreased in Alzheimer's disease (AD) brain. Necroptosis is activated in AD brain and is positively correlated with neuroinflammation and tau pathology. However, the links among altered O-GlcNAcylation, β-amyloid (Aβ) accumulation, and necroptosis are unclear. Here, we found that O-GlcNAcylation plays a protective role in AD by inhibiting necroptosis. Necroptosis was increased in AD patients and AD mouse model compared with controls; however, decreased necroptosis due to O-GlcNAcylation of RIPK3 (receptor-interacting serine/threonine protein kinase 3) was observed in 5xFAD mice with insufficient O-linked β-N-acetylglucosaminase. O-GlcNAcylation of RIPK3 suppresses phosphorylation of RIPK3 and its interaction with RIPK1. Moreover, increased O-GlcNAcylation ameliorated AD pathology, including Aβ burden, neuronal loss, neuroinflammation, and damaged mitochondria and recovered the M2 phenotype and phagocytic activity of microglia. Thus, our data establish the influence of O-GlcNAcylation on Aβ accumulation and neurodegeneration, suggesting O-GlcNAcylation-based treatments as potential interventions for AD.
Human gut microbiota Agathobaculum butyriciproducens improves cognitive impairment in LPS-induced and APP/PS1 mouse models of Alzheimer's disease
No full textAlzheimer's disease (AD) is the most prevalent neurodegenerative disease, and is characterized by the accumulation and presence of amyloid plaques (Aβ), tangles, dementia, and cognitive impairment. Currently, there is no known cure for AD; however, recently, the association between alteration of the gut microbiota and AD pathology has been explored to find novel therapeutic approaches. Microbiota-targeted intervention has been suggested as an attractive therapeutic approach for AD. Agathobaculum butyriciproducens (SR79) is a strict anaerobic and butyric acid-producing bacteria. We hypothesized that administration of SR79 might have a beneficial effect on cognitive deficits and AD pathologies. To determine the therapeutic effects of SR79 on AD pathologies, APP/PS1 transgenic and lipopolysaccharide -induced cognitive impairment mouse models were used. In the lipopolysaccharide -induced cognitive deficit model, the administration of SR79 improved cognitive function and decreased microglia activation. In addition, the administration of SR79 to APP/PS1 mice significantly improved novel object recognition and percent alteration results in novel object recognition and Y-maze alteration tests. Furthermore, Aβ plaque deposition and microglial activation were markedly reduced in the parietal cortex and hippocampus after SR79 treatment in APP/PS1 mice. SR79 treatment significantly decreased gene expression levels of IL-1β and C1QB and increased the gene expression levels of IGF-1 and thereby the downstream signaling pathway in the cortex of APP/PS1 mice. In conclusion, SR79 administration improved cognitive function and AD pathologies through the regulation of neuroinflammation and IGF-1 signaling in an animal model.