Korea Research Institute of Bioscience and Biotechnology
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Inhibitory effect of cephalosporin C on growth of Cephalosporium acremonium M-113
No full textCephalosporin C (CPC) inhibited the growth of Cephalosporium acremonium M-l 13, a potent CPC producer derived from C. acremonium ATCC 20339. Similar inhibitory effects of CPC were also observed in growth of C. acremonium ATCC 20339 and ATCC 14553. Minimum inhibitory concentrations (MIC) of CPC on the growth of conidia and hyphae of C. acremonium M-l 13 were 200-500 and 3000-4000Mg/ml respectively in synthetic medium. MIC values were increased in complex media. The inhibitory effect of CPC was due to CPC-exerted inhibition of amino acids uptake by the cells. 3'-Group of CPC might be important in its inhibitory action. In addition, CPC itself could be utilized by the cells as a nitrogen source under nitrogen limited condition.open
Expression of glucose isomerase gene from Bacillus licheniformis in Escherichia coli.
No full textA Bacillus licheniformis ATCC31667 gene coding for a glucose isomerase has been cloned and expressed in glucose isomerase negative mutant of Escherichia coli. A recombinant plasmid, constructed by ligation of a EcoRI fragment of B. licheniformis chromosomal DNA to vector plasmid pBR322, was expressed glucose isomerase positive in E.coli LE392-6 with growth on minimal medium containing xylose as a sole carbon source. This recombinant plasmid, designated pBGI6, had the insert of 4.1Kb of Bacillus gene in EcoRI site, and restriction map of the plasmid was established. The plasmid pBGI6 was very stable after lOdays of serial transfer to a fresh medium. The activity of glucose isomerase from the transformed cell containing pBGI6 was increased about 20 fold than its wild type of host.open
Cloning of the structural gene for hepatitis B virus surface antigen into a yeast vector
No full textThe structural gene of hepatitis B virus surface antigen (HBsAg) was cloned into a shuttle vector pAAR 6 which is capable of autonomous replication and selection in both of the yeast Saccharomyces cerevisiae and Escherichia coli. This expression vector employs the 5'-flanking region of ADC I gene as a promoter in order for transcribing viral surface antigen coding sequences. After transformation of the recombinant plasmid into yeast strains such as SHY 3, YNN 27, D 13, ATCC 38517 and ATCC 42677, the expression of HBsAg gene in the host cells was evident. The protein synthesized in yeast cells was found to be similar in size, density and shape to the 22 nm particles isolated from the nlasma of human heDatitis carriers.open
Evolution of molecular hydrogen from glucose by Rhodopseudomonas sp. KCTC 1437
No full textRhodopseudomonas sp. KCTC 1437 evolved molecular hydrogen efficiently under light illuminated anaerobic culture conditon in the presence of organic acids and various sugars, especially glucose when low concentration of NH_4 + of L-glutamate was added to cultures. it was revealed that hydrogen formation from Rhodopseudomonas sp. KCTC 1437 was mediated by two different enzyme systems. Under the nitrogen limiting condition, hydrogen evolution from glucose was catalyzed by nitrogenase. For the nitrogenase activation in vivo. the precultured cells grown on limiting concentration of NH_4^+ as a sole nitrogen source showed more capacity of hydrogen evolution from glucose in the presence of L-glutamate than any other cells grown on sufficient concentration of NH_4^+, L-glutamate NH_4^+, or both of L-glutamate and n_2. A significant volume of molecular hydrogen was evolved from glucose even in the presence of excess NH_4^+ either in the light or dark anaerobic condition, presumably due to the mediation of hydrogen evolution by fromic hydrogenlyase.open