Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry)
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ISOLATION AND IDENTIFICATION OF 9-METHOXYCANTHIN-6-0NE COMPOUND FROM Eurycoma longifolia ROOTS
Get PDF9-Methoxycanthin-6-one compound has been isolated from Pasak Bumi (E. Longifolia) roots. The isolation process using maseration, colum chromatography vacuum, and recrystallization techniques. E. Longifolia root maserated with n-hexane and methanol respectively. The methanol extract fractionated using column chromatography vacuum with gradient elution (hexane-etil acetate) to obtain 9 fractions. Fraction 5 further purification using recrystallization technique to obtain yellow crystal, identified by LC-MS and NMR spectroscopy as 9-Methoxycanthin-6-one.Keyword: E. Longifolia, isolation, spectroscopy, 9-Methoxycanthin-6-one
STUDI POTENSI EKSTRAK BRUCEA JAVANICA SEBAGAI BIOAKTIF ANTIKANKER PAYUDARA TERHADAP SEL T47D
No full textresearch. Buah makasar (Brucea javanica) known has anticancer activity.This research would bioassay the anticancer activity ofBrucea javanica extract toward T47D breast cancercell by in-vitro method, using SRB dyed. In order to study the mechanism, a dot blotting method was carried out to observe the interaction between the Brucea javanica extract with the chromosomal DNA molecule of T47D Cell Line. The interaction results were analyzed with High Performance Liquid Chromatography (HPLC) methods.The result of anticancer activity assay toward T47D cell showed that Brucea javanica extract has activity with IC50 2.69 g/mL, whereas, the interactionresult was analyzed with High Performance Liquid Chromatography (HPLC) methods, exhibited the reduction peaks with the retention time 2.26; 3.63 and 3.94 minutes. It was concluded that the bioactive activity ofBrucea javanica due to the presenceof these candidates.Keyword: Anticancer bioactivity, DNA ofT47D ceIl
THE EFFECTS OF LUPIN (Lupinus angustifolius) AS SOYBEAN SUBSTITUTE AND COAGULANT AGENT TO PROTEIN PRECIPITATION IN TOFU PREPARATION
No full textLupinus angustifolius is legume that has high protein. Most of this plant growth in Australia. Another name of L. angustifolius is Australian sweet lupin. L. angustifolius is potentially use as the source of food. Tofu is one of the traditional foods in Indonesia.Tofu is madefrom the coagulation of soybean protein. L. angustifolius has globulin protein that has a role in the production of tofu. The price of L. angustifolius is cheaper than soybean. In this research, L. angustifolius has beenused as the substitution in the tofu production. Thepurpose of this researchis toknow the nutrition and potential of L. angustifolius as the substitute in tofu. This research is also monitoring the effect of coagulant agent that is used in tofu. The research result shows that the optimum concentration substitution of lupin that is used in tofu is 30% with CaCl2 coagulant agent. The tofu that is made from 30% lupin with CaCl2 contained 17.22% protein, 5.23 fat, and 1.14% carbohydrate. The observation shows that tofu that is made from 30% substitution of L. angustifolius has higher protein than tofu which is without substitution of L. angustifolius.Keywords: Lupinus angustifolius, coagulant agent, tofu, protein
AKTIVITAS ANTIOKSIDAN DAN TOKSISITAS EKSTRAK AIR DAN ETANOL DAUN BENALU (Dendrophthoe pentandra L. Miq) YANG TUMBUH PADA BERBAGAI INANG
No full textMistletoes are used traditionally in Indonesiaas medicinal plant, one of this as anticancer. Cancer isone of degenerative diseases could be triggered by toomuch free radicals in the body, whereas antioxidantcould reduced the risk of degenerative diseases.Mistletoes reposted content flavonoids which known have antioxidant activity. Mistletoe potency as drugmaterial should be studied sa the utilization ofmistletoe could be developed. Antioxidant activitytest using "DPPH free radical scavenger" methodand toxicity test using BSLT (Brine Shrimp LethalityTest) method had been conducted on water andethanol extracts of mistletoe Dendropthoe pentandra(L.) Miq.) that grown on various trees (star fruit,mango, cananga, 'duku, sour-sop, kepel, mahkotadewa, and tea). The result showed that ethanol andwater extracte-ofD, petandra on all host plants haveantioxidant activity (IC50 value between 6.4 - 51.8ug/mL). The result of toxicity test showed that ethanolextract of mistletoe on cananga, starfruit, kepel andmahkota dewa host plants have toxicity effect againstA. salina larvae with LCso value below 1000 ug/mL,nevertheless ethanol extract on others host plants andwater extract didn It give toxicity effect against A.salina larvae (LC50 > 1000 ug/mL).Key words: mistletoe, D. petandra, DPPH, antioxidant,toxicity, degenerative disea
AKTIVITAS ANTIDIABETES EKSTRAK AIR Coriandrum Sativum PADA MENCIT JANTAN
Get PDFAntidiabetic activity of aqueous extract from coriandrum sativum had been evaluated by using glucose tolerance test in mice. Mice were divided into five groups randomly and each group consists of six animals. In the glucose tolerance test on mice, 30, 60, 120 and 180 minutes after the administration of the extract at a dose of 8 mg/kgbb, serum glucose concentration decreased by 17.07%, 26.31%, 31.3% and 32.55% respectively. Antidiabetic activity of theextract showed no significant changes to that of control group (p<0.05).Keywords : Coriandrum sativum, water extract, glucose tolerance test, blood glucose level
HOMOGENITAS TARGET DALAM MATRIK PADA ANALISIS RESIDU PESTISIDA KARBAMAT DALAM TOMAT
No full textOptimum condition of carbamate are carbofurane and carbaryl recidues pesticide analysis by height performance liquid crhomatography (HPLC) has been done. The optimum condition are isocratic mobile please on acetonitrile/H20 :45/55 (v/v) and wave lenght of ultra violet (UV) detector has optimum absorbance at 275 nm. Performance of HPLC verification has minimum detection are 60 pbb for carbofurane and 40 ppb for carbaryl. Linierity and work range are 0.06 to 0.15 ppb for carbofurane with r2 value of 0.9702 and 0.04 to 0.15 ppb for carbaryl with r2 value of 0.09874. Precision of HPLC was showed by relative standard deviation (RSD), for carbofurane are 0.30 % of retention time (Rt) and 7.2 % of area. Whereas for carbaryl, the RSD are 0.20 % for Rt and 4.63 % for area. Homogeneity of targets in tomato matric as well as carbaofurane and carbaryl are homogen by analysis of varians (ONOVA) method which. is showed by F-value < F-critis. Homogeneity anlysis by National Acreditation of Comeetee (KAN) method also homogen with criteria I. Keywords: Homogeneity, Pesticide, Carbamate, Carbofurane,Carbaryl, Chromatographs
KARBON AKTIF HASIL DEKOMPOSISI TERMAL LIMBAH KERAK CAT BESERTA KARAKTERISASINYA
Get PDFAutomotive sector which is one of the biggest industry has been producing paint sludge waste in enormous amount that could be dangerous for environment sustainability. To overcome this problem of course waste treatment approach is important, butsomehow the big investment and operational cost is often become restriction in adopting this kind of approach.Another alternative was to process the paint sludge waste onto more useful product (recycle). The large fraction of organic material in paint sludge has leaded an opportunity to recycle this waste ontoactivated carbon through thermal decomposition and activation process. Thermal decomposition process in 310C without activation step has produce activated carbon which has iod number 104.81 mg/g, while the same decomposition process continue with activation step give iod number 91.87 mg/g. These results indicate that paint sludge waste could be recycle onto activated carbon which not only could reduce the environmentpollution but also could give economical benefit. Another characterization also done by DTA and FTIR to get additional information about the surface topography and chemicalfunctional groups of activated carbonproduced.Keyword: adsorben t, activation, thermaldecomposition, activated carbon, paintsludg
ANTI JAMUR HASIL FERMENTASI Streptomyces Isp. 192 PADA MEDIA PATI DAN GLUKOSA
Get PDFStreptomyces ISP 192 was family of Actinomycetes which was high value microorganism that industrially important because it was capablefor producing ofbioactive compound that very useful in the field of medicine that is antibiotic. Recently antibiotic is still eligible medicine to overcome infection disease, but many more organism that cause of infection have been resistant against various antibiotics so need to look for a new antibiotic. This research cover cultivation of Streptomyces ISP 192 in potato dextrose agar (PDA) medium then fermentation by 250 mL shakeflash with 50 ml working volume. Microorganism was inoculated in fermentation medium MF1 and MF2 that contain (%)w/v variation of starch and glucose to bea source of carbon as soon as (%)v/v variation of inoculum. Fermentation process was conducted in 30C temperature with 150 rpm shaking for seven days. Regularly for each 24 hour, sample was taken for analyzing ofpH, glucose and protein concentration, dry weight of cell and testing of anti fungal activity against Candida albicans, Microsporum gypseum, Tricophyton sp. dan Aspergillus niger. The result of research showed Streptomyces ISP 192 has high anti fungal activities against Candida albicans, Microsporum gypseum, Tricophyton sp., in usage of fementation media MF2 with 10 % glucose to be a carbonsource and 10% inoculum but not have activited against Aspergillus niger. Biosynthesis of antibiotic anti fungal start from 4th to 6th day that indication indecreasing of glucose and protein concentration as well aspH.Keywords: Streptomyses ISP 192, fermentation, testing ofantifungal activities