Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry)
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PRODUCTION, ISOLATION, IMMOBILIZATION AND APPLICATION OF GLUCOAMYLASE FROM RHIZOPUS ORYZAE
No full textExperiments were conducted to find optimum medium and process conditions based on shake-flask and laboratory fermentor scales to anticipate industrial scale for the production of glucoamylase. Membrane filtration and ammonium sulfate precipitation were tested for the enzyme concentration and isolation respectively. The glucoamylase enzyme properties were determined and preference to various starch substrates were checked. Immobilization within commercial agar and alginate were done and their shelf-life were observed. The application of free glucoamylase in saccharification of the various starches were also investigated after being liquefied with commercial alpha-amylase.Keywords : rhizopus oryzae, glucoamylase, isolation, immobilization
PEMEKATAN PROTEIN DALAM SUSU DAN KECAP MENGGUNAKAN MEMBRAN SERAT BERONGGA
Get PDFConcentration of protein which found in freshmilk, sweet ketchup and salt ketchup have been done byusing hollow fiber polysulfone membrane with differentmolecular weight cut - off (MWCO). Membranes usedin these experiments have MWCO 66,000, 87,000 and 149,000 Dalton. Experimental were conducted bycontinues flow system and parameters observed in these experimental were protein, sugar and salt, respectively.Protein infresh milk rejected by membrane above 98 %,but all of sugar pass through to the permeate side. Forsweet ketchup feed sample, rejection coefficient towardsprotein was smaller than feed fresh milk. The higherrejection coefficientfor sweet ketchup was 48 % for themembrane with MWCO 66.000 Dalton. Rejectioncoefficient of membrane towards protein for salt ketchup sample was smaller than sweet ketchup sample. For saltketchup, the higher rejection coefficient towards proteinwas 27 % for the membrane with MWCO 66,000Dalton. Rejection coefficient of membrane towardsNaCl for feeding solution sweet ketchup and saltketchup were in the rlJ.1Jgefrom 8.74 to 23.96 %.Key words : Hollow fiber membrane, ultrafiltration, protein,milk, ketchup
PREPARATION OF IN - HOUSE COCOA FLOUR RMs AND DETERMINATION OF ITS Cd CONTENT BY USING GFAAS
No full textIn-house cocoa flour RMs instead of CRMs was prepared and checked of its homogeneity and stability. Due to CRMs are limited availability in terms of matrices of concern and costly (high production cost) making in-house cocaflour RMs is alternatively used for daily quality control in the laboratory. The analysis method for reliable checking of homogeneity and stability of cadmium content in the in-house cocoaflour RMs was studied. The effects of magnesium nitrate and lanthanum/magnesium nitrate as chemical modifiers in the determination of cadmium in cocoaflour by GFAAS were studied as well. Detection limit of method was estimated to be 0.07 ug L'(calcuiaied as 3Sy;/slope of calibration curve) and calibration curve of working range was linear. The results analysis for candidate of in-house cocoaflour RMs reveals the consistence results and no variability between and within-bottle observedat 95% limit of confidence and stabile during the period of time observed
AKTIVITAS ANTIOKSIDAN DAN BIOAKTIVITAS IN VITRO EKSTRAK AIR DAN EKSTRAK ETANOL DAUN DAN RANTING BENALU Macrosolen cochinchinensis (Lour.) van Tiegh. PADA INANG NANGKA ( Artocarpus heterophyllus)
No full textStudy on antioxidant activity and bioactivity of water and ethanol extracts from the leaves and twigs of mistletoe (Macrosolen cochinchinensis (Lour.) Van Tiegh) that grew on jackfruit (Artocarpus heterophyllus) tree as the host have been conducted. Water extracts of the M. cochinchinensis leaves andtwigs were active as antioxidant with IC50 23.08 ug/mL dan 21.56 ug/mL respectively. Whereas the ethanol extracts of both leaves and twigs was not active because the ICso higher than 100 ug/mL (not active as antioxidant against DPPH). Both water and ethanol extracts of M. cochinchinensis leaves and twigs did not showed bioactivity against Artemia salina Leach brine shrimp with each extracts has LCso higher than 1000 pg/mL.Key words Antioxidant, Bioactivity, Macrosolen cochinchinensi (Lour.) van Tiegh., Artocarpus heterophyllus. Artemiasalina Leach
PENGARUH PENINGKATAN LIPOFILISITAS PADA SENYAWA ANALOG UK-3A DALAM MENGHAMBAT PERTUMBUHAN SEL KANKER P-388
Get PDFAntibiotic UK-3A contains a 9-membered dilactone ring. It had been isolated as a minor component from the mycelium of 5trepyomyces sp. 51701.The antibiotic was hypothesized to be potential to inhibit the growth of leukemia cancer cell line of P388 and KB with ICso 38 and 20 Dg/mL, respectively. To understand the effect of lipophilicity increase of the analogues on their anticancer activities based on QSAR parameter (Log P) and binding energy to BcL-xL protein. To produce analogues of UK-3A, 3hydroxypicolinyl serine methyl ester (A) was synthesized from 3-hydroxypicolinic acid and L-serine methyl ester. The product was then esterified by pentanoic (1), hexanoic (2), heptanoic (3), and octanoic (4). The final products were confirmed with IHand 13C FT-NMR and FTIR spectra, and also MS spectra. Then they were tested against P388 Murine Leukemia cells. The result of bioassay showed lipophilicity increase of3hydroxypicolinyl serine methyl octhyl ester (PSMOE) correlated positively with their anticancer activity increase, withICso 15.4mg/mL against P388 cell lines.Keywords: Anticancer, Streptomyces sp 517-02, UK-3A, Analog UK-3A and P38
ANTI BREAST CANCER ACTIVITY OF ETHYL ACETATE EXTRACT OF FERMENTATION BROTH EMPLOYING ENDOPHYTIC FUNGI TAXUS SUMATRANA ISOLATES
Get PDFFermentation broths of TSC 18 and TSC 22 fungi isolates obtained from Taxus sumatrana plant were investigated for their in vitro anti breast cancer activity against T47D cell line. The isolates were further identified by PCR and sequencing methods. In the fermentation experiments, the growth curves were constructed by plate count method and the pH change were recorded, while glucose and protein dynamics of the broth were determined by Nelson-Somogy and Lowry methods respectively. Anti-cancer activity was assessed by SRB (Sulforhodamine B)method. The investigation indicates the fermentation broth both isolates possessed the anticancer activity with lese of 42.6 ppm for TSC 18 and 55.7 ppm for TSC 22. Cisplatin which is used as a reference compound gave ICso of 15.9 ppm . The PCR and tree view program proved that TSC 18 is Phomopsis sp. strain MAFF-238472 and TSC 22 is Coprinopsis cinerrea strain NBRC30628.Keyword: Anticance, Isolates TSC 18 and TSC 22 Taxus Sumatrana
STUDIES ON KINETICS OF DIMETHYL CARBONATE SYNTHESIS BY HOMOGENEOUS TRANSESTERIFICATION
No full textA comprehensive kinetic study on dimethyl carbonate synthesis by transesterification of ethylene carbonate with methanol has been conducted. An alkali base metal (KOH) was used as catalyst in the synthesis of DMC and its catalytic ability was investigated in term of kinetics. The reaction orders, the activation energy and the rate constants were determined for both forward and backward reactions. The reaction is reversible and the equilibrium rate constant wasestimated to be 135.77exp(-16542.4/RT).Keywords : dimethyl carbonate synthesis, transesterification reaction, kinetic study on DMC synthesi
UJI POTENSI AKTIVITAS ANTI KANKER EKSTRAK DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb.) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)
No full textHas been done research to know anticancerpotencial activity from fragrant screw pine leaf extractapplies method Brine Shrimp Lethality Test ( BSLT).Extract is made by the way of macerate to apply threekinds of solvent, that is butanol, ethyl acetate, and etherpetroleum. Toxicity test is done by using prawn larvaArtemia salina Leach which age 48 hours. Toxic effecteach extract is identified with presentase death of prawnlarva applies probit analysis (LC50). Active extract thenis tested its the phytochemistry content and compoundbioa1(fi! suggested applies GC-MS. Result of his (itsshowing ethyl acetate extract to have the character oftoxic ( LC50 : 288,4 ppm). The toxic compounds whichpredietion.implied in ethyl acetate extract is terpenoidsand steroid.Keywords: Fragrant Screw Pine Leaf Extract, BSLT, Artemiasalina LFitokimia, and GC-M
SIFAT FISIKO KIMIA DAN AKTIVITAS ANTIOKSIDAN MINYAK KELAPA MURNI (VCO) HASIL FERMENTASI RHIZOPUS ORIZAE
No full textResearch on physicochemical properties and antioxidant activity of VCO obtained from Rhizopus orizaefermentation had been done. Antioxidant activity was determined by DPPH method. Production of VCO had been carried out from coconut sample obtained from Pandeglang region with fermentation by Rhizopusorizae in variation of inoculum asfollow 2%, 5%, 7%, 10% and 12 %. Physicochemical test consist of density, refractive index, water content, free fatty acid, iodine value, peroxide value while the composition of fatty acid determined by GCMS, The result showed that variation of inoculum had significantly 0,024 influenced the yielded of VCO producted. Based on this result, quality of VCO still in the range of CODEX Standard, 19-1991rev.2-1999. It was found that antioxidant activity of VCO only 6.3% at 1000 ppm. The composition offatty acid based in lauric acid content measured by GCMS showed a significant result (49.48%-50%) agreed with COD EX Standard.Keyword: Antioxidant, DPPH, veo, Fermentation,Rhizopus oriza