Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry)
Not a member yet
300 research outputs found
Sort by
STUDI TENTANG SPESIES LOGAM Ni DARI BEBERAPA KATALIS PREKURSOR Ni/SiO2 TERKALSINASI DENGAN TPR DAN XRD
No full textThree different kinds of Ni-containlng species have been identified in calcined (673K) Ni/SiO2 catalyst precursors according to their different reducibilities during temperature programmed reduction (TPR). Species A is attributed to an easily reduced species, corresponding to the bulk NiO with little interaction with the silica surface; species B is assigned to a less easily reduced form, due to small NiO particle attached to the silica surface; species C is ascribed to a very difficult to reduce species, reflecting to the strong NiO-silica surface interaction, Nickelhydrosilicate. Species A is obtained by either impregnation and ion exchanged methods, while species B and C are only produced by the ion echange method. The retained Na in the ion exchanged-NaOH catalyst affected a decreasing in the proportion of species A and a growth of the proportion of species C. The maximum value of intensity ratios of "d-spacing-XRD " of d2 and d3 in the reduced precursor Ni/SiO2 indicated the highest of catalyst activity
KARAKTERISASI PHARSIAL ISOLATBAKTERI PENDEGRADASI PHENOL DARI TABLET BAKTERI
No full textAn aerobic pure culture (18) was isolated from the tablet of mixed culture bacteria which is originated from a farm land. The gram positive isolate able to degrade phenol about 550 mg/l.d. Morphology of the isolate changes with the culture ages. it was in rod shape during the logarithmic growth phase and changes to the o valform during the stationary phase. The range of temprature growth is 27-34C and the optimum temprature of the isolate is 30C with doubling time about 7 hours. Substrate could be utilized by the isolate are alcohol, aromatic compound and carbohydrate. Methanol, ethanol, isopropanol (each 10%; v/v), 4-Chlor phenol (0.2 mM), 4-nitrophenol(0.25 mM), anniline (1 mM) and glucose are some substrate has been tested for the bacterial growing. Salt (NaCl) tolerance for the isolate is up to 2% (v/v) and the addition of ammonium salt would be support the bacterial growthKey words:Bacterial tablet, aerobic, phenol degradation, 4-chlor phenol, 4-nitro phenol, anniline, glucose, methanol, ethanol, iso-propanol, isolation, partial characterization
Identification and Characterization of Bacterial Lipase from Plateu Soil in West Java
No full textLipases are known as glycerol ester hydrolases that catalyze the hydrolysis of triglycerides into free fatty acids and glycerol. Lipases are found in human, animal, plant, and microorganisms. The aim of this research is to identify lipase producers and characterize bacterial lipase from West Java plateau soil. Plateau soil bacteria samples were isolated on lipase screening medium containing Rhodamine B. Olive oil was used as a substrate in screening and production medium bacterial lipases. From 16 bacterial isolate of lipase producers, 14 were identified as Bacillus sp. and the others were identified as Pseudomonas alcaligenes. All isolates were taken into production step to determine their lipase activities. Moreover, top 3 lipase activities out of 16 lipase activities were chosen to find the optimum pH and temperature. Both characterizations showed pH optimum and temperature optimum from each lipase. These optimum condition were used in heat stability characterization for each lipase samples. The result showed that lipase from isolate COK 2 in optimum pH 4 and temperature 50oC was the most stable lipase due to this sample has good and stable activity for 1 to 5 hours incubation time. Lipase sample from isolate COK 2 has good efficiency for lipase productivity in acid condition and high temperature. Results of this investigation could encourage utilization of these activity enhancers for various industrial applications
PREPARASI DAN KARAKTERISASI KATALIS PEROVSKITE (La,Sr)MnO3+)"
No full textThe LaMnO3 and La0.8Sr0.2MnO3+A perovskites were synthesized base on sol-gel method using metal nitrates in required proportions. In the preparation, ethylene-glycol and water were used as the dissolving agents and comparison was made. The amorph gel was obtained from nitrate mixture solutions heated in a range 60-80C, either under vacuum conditions in rotary evaporator or under normal pressure (atmospheric). Perovskite crystals were obtained from calcination process of the amorph gel, and the product was further characterized by X-ray Diffraction analyzer, BET method, Scanning Electron Microscopy as well as Thermal analyzer (TG-DTA). With regard to the process and characterizations, the product obtained by using ethylene-glycol as the dissolving agent was relatively better than. the one produced in aqueous condition. A specific surface area of the sample with ethylene-glycol as dissolving agent (26 m2/gr.) was wider than the sample prepared in aqueous solution (16 m2/gr.). The percentage of weight loss during the formation of perovskite powder was 50 %, it consisted of water, nitrogen and organic compounds. From the X-ray Diffraction analysis, the structure of the crystalline perovskite was obtained by calcination process at 600C was rhombohedric with a = 7.7 A and (X, = 90.39. Oxidation reaction of Carbon Monoxide, Hydrocarbon and Particulate of Diesel Engine on these Perovskite was very much affected by specific surface area, structure and Mn 4+ ion
SINTESIS SENYAWA S-BENZOIL-MAG3 UNTUK PEMBUATAN KOMPLEK 99mTC-MAG3
No full textSYNTHESIS OF S-BENZOLY-MAG3 FOR THE PREPARATION OF 99mTc-MAC3 COMPLEX. 99mTc-MAG3 is a new radiopharmaceutical having similar biological properties to 131Ilabeled. Hippuran and is expected to be useful as an ideal agent for renal funtion evaluation. In order to develop the abovepreparation, it is necessary to synthesize S-benzoly-MA3 ligan. In the present study the synthesis was carried out in a 3 stepsreaction of succinimiidyl-S-benzoylthinglycolic acid from S-benzoyltingioglycolic acid, N-hydroxysuccinimide and N,N' -dicyclohexylcarbodiimide. The last step is syntesis of benzoyl-MAG3 from siccinimidyl-S-benzoylthioglycolate and glycylglycylglycine. The product has a crystaline from, colorless needles with a melting point of 203-204C Purity testing by HPLC yielded a single peak, indicating a sufficiently pure compound. IR spectrophotometry provides proof that the compound is S-benzoyl-MAG3
PENGARUH PENGGUNAAN GARAM NATRIUM DAN KALIUM METABISULFIT PADA MUTU SALE PISANG AMBON
Get PDFTechniques of improving the colour, aroma, texture and storage life of pisang sale or dried banana (Musa paradisiacal was conducted through sulphiting and drying. In this study, the sulphiting of ball all a fruit was done by soaking the fruit in O.S% sodium meta bisulfite solution (NazSzOs) or O.S% potasium metabisulfite solution (K2S2O5) for five minutes. Dehydration was conducted at 6OC for 44 hours in a tray dryer. The dried banana was put in plastic bag of O.5 mm film thickness and stored in a rack covered with transparent plastic film at ambient temperature (25 2C) and relative humidity of 70%. The experiment was done using random block design. Analysis of the dried banana products included chemical, microbiological an d organoleptic methods after storage for one day, three weeks, six weeks and 12 weeks. The results indicated that the artificial drying coupled with the metablsulphite treatment could improve the quality and shelf life of the dried banan a produced. The product quality characteristics include: water content (23-27%), total reducing sugar (41-49%), SO2 (3-S fpm), color/optical density (0,3-0,7); and total colony count (7-7.5 x 10(3)) per gram sample for the products stored up to 12 weeks
BIOOEGRAOASI SENYAWA AROMATIK OLEH PSEUDOMONAS SP ISOLAT I4 DARI TABLET BAKTERI
Get PDFBiodegradation test of aromatic compounds by pure cultureof bacterial isolate I4 from bacterial tablet using mineral mediumas growth medium was done. Aromatic compounds tested ascarbon sources used were phenol, p-chlorphenol, p-nitrophenol,2,4 dichlorphenol and benzene. The oval colony forming and highmotility isolate is an aerobic and gram negatif bacteria. Theisolate was able to use citric acid as carbon source, producedacid .from methyl red test and gave negative result of indol test.The isolate formed a slightly blue-green colour colony on agarslant, and it did not use ethanol as carbon source. Thecharacteristic of the isolate I4 indicated that it belonged to thegenus Pseudomonas. Test using selective media confirmed thatthe isolate I4 is a Pseudomonas fluorescence. The capability ofthe bacteria to oxidize of 2,4 dichlorphenol was relatively fast.Experimental results showed that the bacteri grew on benzene atconcentration of 10% (v/v), and degraded 2,4 dichlophenol withrelatively high rate.Keywords: Bacterial tablet, aromatic compounds, phenol. pchlorphenol,2,4 dichlorphenol, p-nitrophenol, benzene, pure culture,Pseudomonas fluorescence, isolate I
PRODUKSI GLUKOAMILASE DARI RHIZOPUS ORYZAE SKALA FERMENTASI 2 LITER DAN 4 LITER
Get PDFProduction of glucoamylase by R. oryzae has been conducted in erlenmeyer flasks using medium containing sago starch as carbon source and soybean meal as nitrogen source. It was known that 2 % of sago starch and 0.5 % of soybean meal in the medium is the best composition for the production of glucoamylase. At the present study, the optimal condition for maximal production of glucoamylase fermentation from R. oryzae was investigated using sago starch medium in the 2L and 4L scale. The results showed that the maximum production of glucoamylase at 600-1500 ml fermentation scale was reached at day-8 of incubation time. At this condition, the enzyme specific activity was 0.85 U/mg protein - 1.50 U/mg protein. Forglucoamylase production within 4000 ml fermentation scale, the maximum enzyme specific activity, 2.58 U/mg protein, was obtained at day-S of fermentation with 300 rpm agitation, while the maximum activity of 3.47 U/mg protein and 4.71 Ulmg protein were achieved at day-6 and day-5 of fermentation process with 500 rpm and 700 rpm agitation, respectively. At this maximum condition, the use of sago starch reached 94 - 98 %, and biomass production at the end of fermentation process was 4.80 - 7.90 g [dry weight)/L medium