Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry)
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PEMBENTUKAN, PENGUJIAN AKTIFITAS DAN SELEKTIFITAS BEBERAPA KATALIS PREKURSOR DARI SENYAWA "Ru-KETENILIDINE/SiO2"
No full textThe catalyst precursor of Ru3CCO/Si02, RU3C:/SiO2 and RU3:/SiO2 had been identified by "Temperature-Programmed Desorption(TPD)-FTIR-insitu" techniques. The last two precur- sors show a reversible reaction with CO, but the RU3CCO/SiO2 is rather unstable. Test of precursors with C2H4, shows that Ru3CCO/SiO2 produced trans-C4Hg, RU3:/SiO2 produced cis-C4Hg and the RU3C:/SiO2 yielded C3H6, selectively
STUDI KIMIA SENYAWA GLIKOSIDA TUMBUHAN SUNGKEI, PERONEMA CANESCENS (VERBENACEAE)
No full textTwo glycoside compounds from methanol extract of Peronema canescens Jack. (Verbenaceae) were isolated and elucidated using on the basis of Nuclear magnetic resonances (1H-, 13C-NMR), mass and ultra-violet (with diagnostic reagent)
PENGEMBANGAN METODA ANALISA KIMIA UNTUK PEMANTAUAN PROSES FERMENTASI PEMBUATAN ASAM CUKA, ANTIBIOTIKA DAN HORMON STEROID
No full textChemical analysis plays an important role in monitoring fermentationprocess and determining product quality of the process. Nowadays the development of analytical methods, which commenced from relatively conventional method to instrumental method, has become a reality. The development does not only increase either sensitivity or reproducibility, but also could identify the existence of substances produced during fermentation, which could not be achieved by conventional methods. In this article, several chemical analyses used for monitoring the production of vinegar, antibiotic and steroid harmon are described. In vinegar fermentation, analyses cover the determination of sllgars, ethanol and organic acids, while in antibiotic fermentation, in addition to determination of sugar, analyses of tetracycline derivatives as fermentation products, is also carried out. In steroid fermentation, analysis covers the determination of solasodine as substrate, AD and ADD as fermentation products
PEMBUATAN SENYAWA AMINA SEKUNDER DENGAN PROSES HIDROGENASI SENYAWA NITRIL
No full textThe amine compounds are used excessively in chemical industry, mainly as a surfactant. Indonesia imports this compound in a large amount (14.000 tons). The secondary amine is an intermediate product of quartener amine which is known as a softener. This research is aimed to observe the chemical reaction of synthesizing secondary amine at laboratory scale.To achieve this goal, the synthesize was conducted by hydrogenating the nitrile compound in a reactor in which two levels of pressure were applied (11 and 35 bars). The reaction temperature as well as the length of reaction time were also varied i.e at 140, 160 and 175 Cfor 3, 6 and 10 hours.The analytical results showed that at relatively low pressure (11 bars) and the reaction temperatures of 140,160 and 175 C for 10 haurs gave products containing secondary amine as much as 48,90; 74,63 and 67,26%, respectively. The 6 hours processing time at 140C did not produce any amine. However when temperature was increased to 160 and 175 C, the amounts of amine yielded are as follow: 46,40 and 73,33%. A higher result (85,80%) was obtained from a process conducted at reaction temperature 175 C for 3 hours.By increasing the pressure up to 35 bars and the reaction was maintained at 160, 83,40% amine was produced in 1 hour only. This finding indicated that producing amine at lower pressure required longer processing time
PERAN ILMU PENGETAHUAN DALAM MEMPERKUAT UPAYA PENGENDALIAN PENCEMARAN LINGKUNGAN YANG EFEKTIF
No full textISOLASI ENZIM HORSERADISH PEROKSIDASE (HRP) DARI KULTUR SEL DAUN ARMORACIA LAPATIFOLIA DENGAN CARA FRAKSINASI MENGGUNAKAN AMONIUM SULFAT
Get PDFIsolation of HRP ( Horseradish Peroksidase ) from Armoracia lapatifolia plant cell culture was conducted by fractionation using ammonium sulfate. Optimation of thefractionation process was carried out by concentrating the crude enzyme, investigating the fractionation stages and precipitation with ammonium sulfate. Two-stage fractionation namely with 0 - 40% and 40 - 85 % saturation of ammonium sulfate gave better results in terms of enzyme activity. In 0 - 40% saturation, the purity was 23fold with 11.4 %yield. The 20 highest yield, 60% and purify of 43 folds were showed by fraction which were obtained at fractionation 40 - 85 % saturation.Keyword: Horseradish Peroksidase, Annoracia Lapatifoli
SOL-GEL PROCESS FOR THE MANUFACTURING OF TRANSLUCENT LEAD ZIRCONATE TITANATE GEL-MONOLITH
Get PDFTranslucent Lead Zirconate Titanate (PZT) gel-monolith was prepared by partially hydrolyzing metal alkoxides solution which modified with acetylacetone(acacH). Metal alkoxides, lead di-i-propoxide, zirconium tetra-nbutoxide and titanium tetra-i-propoxide were used as starting materials. In Infrared spectra for the translucent monolithic gel after aging at room temperature for several days or drying at 90C for 18h, the most significant feature is the presence of band at around 1554 cm-1 which can be assigned to the v (C-O) and v (C-C) vibrati-ons of acetylacetanate group coordinated to the metal cations Ti and Zr. The diffraction peaks of PbO were found after heating at 300C for 2h. After heating at 450 C for 2 h, diffraction peaks of pyrochlore Pb2 Ti206 and perovskite PZT phase were observed. The diffraction peaks of PbO and pyrochlore phase disappeared after heating at 600C, and tetragonal perovskite phase was stable up to 1000 C. The diffraction peaks of perovskite phase were also found after heating at 430 for 24 h. The density of the compacted pulverizedgel after heating at 1000C for 30 min. was 6.9 g/cm3 , about 86% of the theoretical value
KESTABILAN AKTIVITAS ANTIBAKTERI HASIL FERMENTASI MOLASE OLEH STREPTOMYCES RIMOSUS ATCC 33022
Get PDFMolases and ammonium sulfate can be used as medium component to supply carbon and nitrogen respectively for production of antibacterial, through fermentation by Sirimosus ATCC 33022. During fermentation, pH, biomass and antibacterial activity against Bacillus cereus were observed. The results of this investigation indicated that the antibacterial production started after 48 hours of incubation and the maximum production was achieved after 144 hours of incubation. The antibacterial agent was extracted with n-butanol of technical grade and HCI 0.1 N and purification was carried out by recrystallization with ethanol as the solvent. The yield was 50.278 mg antibacterial agent per litre of fermentation medium. The isolated antibacterial compound possesses Amax of 266 nm whereas the oxytetracycline demonstrated maximum peak at 267nm with Eo 1.8529 x 10(4) cm(-l) M(-1). Thin layer chromatography with three eluents of the standard oxytetracycline gave Rf of 0.76; 0.64 and 0.75 while the isolated compound gave 0.76; 0.65 and 0.77 for the respective eluent. HPLC analysis on J-l-bondapak C-18 column with methanol-acetonitrile-oxalic acid 0.1 M (1 : 15 : 7) being the solvent indicated that the isolated antibacterial agent and oxytetracycline standard were eluted at 4.35 minutes. Further analysis with FTIR of both isolated antibacterial agent and oxytetracycline standard had peaks at 3400, 1650-1600, 1238 and 2916-2848 cm(-1) indicating the presence of fenol, amide, amine and methyl groups. Antimicrobial activity tests during storage at 4 C and -12C of the isolated compound in various HCI solutions showed that HCI 0.1 N gave the best stability
ISOLASI GENISTEIN DARI TEMPE SECARA KROMATOGRAFI
Get PDFThe presence of genisteln in tempe (fermented soybean) could be detected by using various chromatographic techniques, such as Thin-Layer, Column and High Pressure Liquid Chromatography. In this experiment, TLC was chosen for the isolation and purification of genistein from tempe. Spectrophotometry, Mass Spectrometry, spraying with chromogenic reagents and hydro- lysis were carried out for identifying genistein. Quantification was carried out spectrophotometrlcally and it was shown that the laboratory prepared tempe (l00 g) contained 13.80 mg ofgenisteln
PENGGUNAAN EKSTRAKSI FASA PADAT UNTUK ANALISIS TETRASIKLIN DALAM CONTOH UDANG
No full textA great quantity of Indonesian frozen prawns were exported to Japan and America. Unfortunately these products have often been rejected due to their content of tetracycline derivative residues. Qualitative analysis of frozen prawn samples being exported by means of HPLC, indicated that they are contaminated by oxytetracycline and tetracycline residues. A problem of quantitative analysis of such residues could be due to several peaks of the matrix being eluted closely to the peaks of the tetracycline derivatives. An experiment was carried out to eliminate the peaks of the matrix origin using SPE (Solid Phase Extraction) in order to quantify the derivatives more accurately. Application of SPE in the sample pretreatment is useful not only for separating the solute being analyzed from the matrixs, but also for concentrating the tetracycline derivatives of the extract. The recovery of SPE column elution process was about 90% and the SPE octadecyl (1 ml) column capacity for oxytetracycline, tetracycline, demeclocycline and doxycycline i.e. 2.4-7.9 ug; 3.5-11.8 ug; 3.4-11.2 ug and 17.3-57.5 ug respectively