Journal of Advanced Laboratory Research in Biology
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    100 research outputs found

    The Effect of Different Organic Fertilizers in Various Compactness Levels on the Amount of Some Nutrients of Sport Lawn in spring

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    Lawn has an incredible role in designing and construction of landscape areas and lawn bed provides aeration, moisture and nutrients, which is essential for having an acceptance lawn, so the role of organic matter and degree of soil compactness need to be studied. In the current study, the effect of some bed mixtures including Leaf Mold (LM), Rice Husk (RH), manure, Spent Mushroom Compost (SMC), a mixture of LM, RH and SMC (mixture 1), a mixture of LM, RH and manure (mixture 2), with the ratio of 1:1:1 and control (no fertilizer), also the effect of some different soil compactness (roller weights of 36, 56 and 76 kilograms) on the content of some nutrients of sport lawn in spring season were investigated. Hence, an experiment was conducted as the strip plot design in three replications, in research farm of Gorgan University of Agricultural Sciences and Natural Resources during 2008-09. According to the results of this study, interaction of fertilizer and soil compactness was significant for most measured factors so that treatments containing manure showed the most nitrogen content in all three compactness levels. As well, in all three compactness levels, the high phosphorus content of plant was also found in manure treatment and the lowest in control and LM. Furthermore, manure, SMC, and treatments containing these two organic fertilizers showed increased potassium of plant toward control and LM

    Induction of Carbendazim Resistance in Aspergillus niger through Mutagens

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    The sample of Ziziphus fruits infected with different pathogens was collected from the market at different places in Maharashtra. Aspergillus niger was found to be dominant all maximum fruits. Development of Carbendazim resistance in Aspergillus niger due to spontaneous mutation. Ultraviolet rays and chemical mutagens such as like bromyl uracil (BU), ethyl methanesulfonate (EMS) and UV rays, same isolates were highly resistance, some were moderately resistance while few were resistance, highly resistance mutant were more pathogenic. Most sensitive isolates of Aspergillus niger A-9 was selected for this study. The spores of this isolate were treated with UV rays, bromyl uracil & ethyl methanesulfonate, it was seen that EMS treatment gave higher mutants followed by UV, Bu and spontaneous treatment in decreasing manner

    Quinalphos Induced Antioxidant Status and Histopathological Changes in the Gill of the Freshwater Fish, Oreochromis mossambicus

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    To extend the knowledge about quinalphos induced antioxidant status and its related changes on the histopathology of gills, the freshwater fish Oreochromis mossambicus was chosen as a model system. Quinalphos treatment (0.5μl/ L for 30 and 60 days) decreased the activities of antioxidant enzymes with concomitant increase in the production of malondialdehyde. Increased reactive oxygen species generation coincides with the increase in the protein carbonyl in the gills of the treated fishes. Histological observation in the gill of quinalphos treated animal for 60 days showed several alterations as hypertrophy of gill arches, lifting of lamellar epithelium, degeneration of gill filament and lamellar epithelium and vasodilation in the lamellar axis when compared to the control group. These observations suggest that chronic exposure to pesticide affect the respiratory oxidative potential of the freshwater fish and this could be possibly due to quinalphos induced oxidative stress in the gill

    Water Quality Index for Assessment of Water Quality of Duhok Lake, Kurdistan Region of Iraq

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    Water quality of Duhok Lake was evaluated by Water Quality Index (WQI) technique. A water quality index provides a single number that expresses the overall water quality at a certain location and time based on several water quality parameters. The objective of an index is to turn complex water quality data into information that is understandable and usable by the public. Ten most important parameters such as pH, electrical conductivity (EC), total hardness, total alkalinity, dissolved oxygen (DO), calcium (Ca), magnesium (Mg), sodium (Na), potassium (K), and sulfate (SO4) were taken for the calculation of WQI. The result indicated that water quality of Duhok Lake poor in the years 1999, 2000 and is poor in 2009 too. The impact of various anthropogenic activities was evident on some parameters such as the EC, alkalinity and hardness. It is suggested that monitoring of the lake is necessary for proper management. Application of the WQI is also suggested as a very helpful tool that enables the public and decision-makers to evaluate water quality of lakes in Iraq

    The Effect of Fertilizer Treatments at Three Compactness Levels on Qualitative Traits of Sport Lawn in Winter Season

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    Lawn quality, which introduces by good color, density, uniformity and texture varies depending on the species, maintenance operation, and time of the year. In the current study, the effect of organic fertilizers containing Leaf Mold (LM), Rice Husk (RH), manure, Spent Mushroom Compost (SMC), a mixture of LM, RH and SMC (mixture 1), a mixture of LM, RH and manure (mixture 2), with the ratio of 1:1:1 and control (no fertilizer) at three levels of soil compactness including roller weights of 36, 56, 76 kilograms on qualitative traits of sport lawn were investigated in winter season. Treatments were applied in a strip plot design with three replications, in research farm of Gorgan University of Agricultural Sciences and Natural Resources during fall 2008-2009. According to achieved results, manure and RH treatments in first and second compaction and manure treatment in third compaction level showed the highest amount of chlorophyll content. In visual assessment, the highest and lowest of lawn quality was observed in the manure and the control treatment respectively. In first and second compaction, the maximum height was observed in manure treatment. Control and LM treatments showed the minimum height. In third compaction, maximum and minimum heights were associated with manure and control treatment respectively

    Traditional Dye Yielding Plants of Firozabad, U.P., India

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    District Firozabad is a city of glass work and Bangles. It is also a rich source of ethnobotanical studies. Local weavers and ethnic communities use plant dyes for imparting different shades to their clothes, hands, hairs and food items. About 19 plant species belonging to 17 families of Dicots, mostly trees and herbs have been identified as traditional dye yielding plants

    The Effect of Mulch, Pruning and Plant Density on Some Traits of Related to Production in Pepino

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    Pepino (Solanum muricatum) a new vegetable crops, is from Solanaceae family and cultivated as annual crops. In order to investigate the effects of mulch, pruning and plant density on some traits of related to production in Pepino, an experiment was conducted based on a randomized complete design with five replications at the Ferdowsi University of Mashhad greenhouse during 2009-2010. Traits such as leaf area, number of panicles per plant, number of fruits per plant, fruit length, fruit diameter and fruit weight. Treatment included: 3 levels of mulch (rice straw mulch, wood chips mulch and control), 3 levels of prune (two peduncular, 3 peduncular and controls) and 2 levels of plant density (3.3 and 4.3 plants per m2). The results indicated that the effect of mulch in all of the traits was significant (p≤0.01). Wood chips mulch in all of the traits was better than rice straw and control. Pruning was significant in all of the traits. The number of panicles and the number of fruits per plant were higher in tree peduncular but increasing the number of fruits was associated with a reduction in size. Interaction between mulch and pruning was significant in all of the traits. Plant density applied had significant effects on traits related to fruit

    Unripe Fruit's Extract of Quince (Cydonia oblonga Miller) as a Potent Alpha-amylase Inhibitor

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    The use of alpha-amylase inhibitors has recently gained in popularity with the success and growth of carbohydrate restricted diets. In this study, two different stages from the unripe fruits of quince (Cydonia oblonga Miller) have been tested for their potentiality in alpha-amylase inhibition as a key enzyme in carbohydrates assimilation. Our results revealed that addition of different concentrations from extracts (0, 2, 4, 6, 8mg) of dry mass of each stage of unripe fruits resulted in drastically decrease in the enzymatic activity of alpha-amylase by the percent of (0%, 42.6%, 21%, 26.3%, and 16.9%) for the stage 1. Extracts from the stage 2 were more effective in enzymatic inhibition (0%, 26.9%, 3.8%, 0.2%, and 0.4%). The GC/MS analysis revealed that quince extract contains (sorbitol, quinic acid, p-vinylphenol and cyclopropane carboxylic acid). To explore which components are involved in the inhibition process, two pure components of the quince extract (sorbitol and quinic acid) were used in inhibition assay. Neither sorbitol nor quinic acid shows any significant inhibition; therefore, these two components could be excluded from the inhibition process. Our current study suggested that p-vinylphenol and cyclopropane carboxylic acid might act as a-amylase inhibitors in vitro separately or synergistically. The possible explanation for the presence of cyclopropane carboxylic acid (CPCA) in this critical phase of the unripe fruit will be discussed. This study suggests that the unripe fruits of quince can be used as a natural starch blocker containing alpha-amylase inhibitors which would be of interest for people requiring carbohydrate restricted diets

    Biodiversity and Concentration of Airborne Fungi of Suburban Weekly Market Associated Environment

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    It is supposed that the vegetable markets of tropical countries are the significant source of airborne fungal spores, which are generated during transportation, handling and putrefaction. The aim of this work was to monitor the prevalence of the airborne cultivable fungi in the air of weekly market associated environments to evaluate whether the vegetable trading zone of market is a source of airborne cultivable fungal spores of weekly market environment or not. Airborne cultivable fungal spore levels were monitored by using Andersen two-stage viable (microbial) particle sampler. The Spearman correlation coefficients and stepwise linear regression analysis test was used to analyze the influence of meteorological factors on spore concentration and paired Student’s t-test was used to compare the bioload of total viable cultivable fungi of vegetable trading area and general item trading area of weekly market extramural environment, the percentage frequency and the percentage contribution of the individual genus was also reported. In both areas, Aspergillus, Cladosporium, Alternaria, and Penicillium, were the most abundant fungal types observed. The spp. of Candida was reported only at the vegetable trading area. The bioload of fungal spore presented maximum values during the Monsoon and lowest in the season of summer. There is no significant difference in quantity between the mean values of the bioload of total viable cultivable fungi of vegetable trading area and general item trading area of weekly market extramural environment were observed. For present environment, activities of animals and humans were supposed to be the key factor governing aerosolization of microorganism

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