Journal of Advanced Laboratory Research in Biology
Not a member yet
100 research outputs found
Sort by
Remarkable Description of a New Species of Apocryptus, 1932
Get PDFThe genus Apocryptus was established by Uchida in 1932 for the species Apocryptus issikii. The genus is distinguished by truncate apical margin with a pair of tubercles at the middle. The genus is known by two species viz., Apocryptus tikari Gupta & Gupta, 1983 and Apocryptus flavofacies Gupta & Gupta, 1983 from India. A new species of Apocryptus, Apocryptus indicus is described from India as a key for identification
Adsorption of Dyes from Aqueous Solution Using Orange Peels: Kinetics and Equilibrium
Get PDFThe adsorption of dyes from aqueous solution has been investigated on Orange Peels (OP). The influence of pH, contact time, adsorbent dosage, dye concentration, agitation speed, temperature and particle size of biosorbent on the biosorption has been investigated. The biosorptive capacities of the biosorbents were dependent on the pH of the dye solution, with pH 2 and 4 being optimal. From the analysis of isotherm data obtained from the fittings of the Freundlich model fits better than the Langmuir model in case of acid blue MTR dye and in case of red 3BLS disperse dye Langmuir model fits better than Freundlich model. The adsorption capacities were found to be 19.48, 36.67, 16.69mg/g for FOP, IOP, DOP in case of Acid Blue MTR Dye and similarly 23.35, 29.36, 27.30mg/g respectively in the case of Red 3BLS Disperse Dye
A Contribution to Algal Flora in Baghdad Area, Iraq
Get PDFThis study contains the description of twenty-five algal species, twenty genera, nineteen families, nine orders and six classes that were identified in the Baghdad Area during the period from 2007 till 2009, and based on the study two new records will be added to the Algal Flora list of Iraq
Reduction of Allergenicity of Litchi chinensis Flowers Pollen Protein Conjugated with Polysaccharide by Maillard Reaction
Get PDFBackground: Allergy to pollen from gymnosperms is well documented in the west. The objective was to define the allergologic protein from Litchi chinensis (Litchi) pollen and conjugate the protein with polysaccharides by Maillard reaction to reduce the allergic effect of that protein.
Methods: Total soluble proteins were extracted from the pollen of Litchi flower pollen and subjected to ammonium sulphate precipitation at 80% saturation. Pollen antigen from Litchi chinensis (Litchi) was prepared by gel cutting method and characterized by biochemical and designated by LFPP. The homogeneity of this protein was demonstrated by a single band on SDS-PAGE. The protein then conjugated with galactomannan through Maillard Reaction. The resulting purified pollen protein and conjugated protein were administered to the Swiss albino mice as amount of 5.8mg/kg body weight.
Results: The total protein was then separated on a 12% SDS-Polyacrylamide gel which revealed 5 bands between molecular weight range of 29kDa and 69kDa. Each band was recovered from the gel by electroelution and sent for skin tests. 28kDa proteins was the only allergenic protein while others were not shown reactivity in patients. Intraperitoneal injection of the purified protein (LFPP) caused a significant rise in the levels of neutrophils (38-81%) and eosinophils (3-14%) compared to control (P<0.001) whereas conjugated protein caused only a 2% increase of both neutrophils and eosinophils level. On the other hand treatment with LFPP-galactomannan conjugate causes no such change in physical appearance with eosinophils and neutrophils level.
Conclusion: The present study demonstrates that the protein extracted and purified from Litchi flowers pollen has been recognized as a new allergen from Bangladesh for the first time and the allergic effects can be reduced by conjugation with polysaccharides
Diversity of Endophytic Actinomycetes from Wheat and its Potential as Plant Growth Promoting and Biocontrol Agents
Get PDFA total of 35 endophytic actinomycetes strains was isolated from the roots, stems and leaves tissues of healthy wheat plants and identified as Streptomyces sp. (24), Actinopolyspora sp. (3), Nocardia sp. (4), Saccharopolyspora sp. (2) Pseudonocardia (1) and Micromonospora sp. (1). Seventeen endophytic actinomycetes isolate showed abilities to solubilize phosphate and produce IAA in the range of 5 to 42mg/100ml and 18-42µg/ml respectively. Nineteen isolates produced catechol-type of siderophore ranging between 1.3-20.32µg/ml. Also, hydroxamate-type siderophore produced by 9 isolates in the range of 13.33-50.66µg/ml. Maximum catechol-type of siderophore production was observed in Streptomyces roseosporus W9 (20.32µg/ml) which was also displaying maximum antagonistic activity against ten different pathogenic fungi. The results indicated that internal tissues of healthy wheat plants exhibited endophytic actinomycetes diversity not only in terms of different types of isolates but also in terms of functional diversity
Total Serum Cholesterol Level in Cases of Carcinoma Breast – A Correlative Study
Get PDFAims and Objective: Correlation between serum cholesterol level and risk of breast cancer.
Material and Methods: Fifty histologically diagnosed breast cancer patients were included in the study. Patients were grouped according to the size, histological type of the tumor and lymph node involvement. Patient’s total serum cholesterol levels were measured a week prior to the operation and on the 14th day postoperatively. Fifty age-matched non-cancer females were treated as a control.
Observations: The pre and postoperative mean total serum cholesterol (MTSC) was found to be 272.3 ± 43.4mg/dl and 210.54 ± 31.5mg/dl respectively. An MTSC level in fifty control cases was found to be 183.42 ± 34.4mg/dl. Patients with tumor size less than 5cms showed preoperative mean serum cholesterol levels of 261.6 ± 36.5mg/dl and a postoperative value of 201.9 ± 16.9mg/dl while those with tumor size more than 10cms showed a mean serum cholesterol level of 282.36 ± 64.8mg/dl preoperatively and 211.82 ± 53.15mg/dl postoperatively. Thirty patients with scirrhous type of carcinoma had a preoperative mean serum cholesterol level as 237.36mg/dL, and twelve patients with a colloid type of carcinoma showed a preoperative level of 294.9 ± 18.4mg/dL whereas eight patients with medullary type of carcinoma showed the highest preoperative level of 324.3 ± 19.8mg/dL, whereas the corresponding postoperative values were 202.9 ± 30.0mg, 216.8 ± 27.8mg and 229.4 ± 29.8mg respectively.
Conclusions: The present study helps us to conclude that dietary habits affect the association of cholesterol in breast cancer and size, histology with tumor metastasis effects such relationship
Biosand Filter for Removal of Chemical Contaminants from Water
Get PDFNumerous reports by the United Nations and the World Health Organization have indicated a significant worldwide problem with water pollution and inaccessibility to potable drinking water. Due to technological and economical barriers, the problem with water pollution is particularly more serious for under-developed and developing countries. The present study is aimed at designing, constructing and evaluating a cost-effective biosand filter was undertaken. Results indicated the removal of up to 80% total hardness, 86% chlorides, 96% turbidity and 90% colour. Moreover, the filter’s performance was appraised by the absence of E. coli in the filtered sample. The filter describes the proven bioremediation technology and its ability to empower at-risk populations to use naturally occurring biology and readily available materials as a sustainable way to achieve the health benefits of safe drinking water
Evaluation of Chitin as Natural Coagulant in Water Treatment
Get PDFThe use of synthetic coagulants is not regarded as suitable due to health and economic considerations. The present study was aimed to investigate the effects of alum as coagulant in conjunction with chitin as coagulant aid on the removal of turbidity, hardness and Escherichia coli from water. A conventional jar test apparatus was employed for the tests. The experiment was conducted at three different pH conditions of 6, 7 and 8. The dosages chosen were 0.5, 1, 1.5 and 2mg/l. The results showed that turbidity decrease provided also a primary Escherichia coli reduction. Hardness removal efficiency was observed to be 93% at pH 7 with 1mg/l concentration by alum whereas chitin was stable at all the pH ranges showing highest removal at 1 and 1.5mg/l with pH 7. At low concentration chitin showed marginally better performance on hardness. In conclusion, using natural coagulants results in considerable savings in chemicals and sludge handling cost may be achieved
Study the Response of Cucumber Plant to Different Magnetic Fields
Get PDFMagnetic fields (MF) are widely distributed in the environment and their effects are increasing due to various instruments that are used in industry and medicine. In the present experiment, the growth and productivity of cucumber plants of seeds in which affected by different magnetic fields was investigated. The soaked seed samples of cucumber were exposed to a 20μT AC magnetic field for 30 minutes. Similar seed samples also were treated with DC magnetic fields of 5μT for 30 min. To compare the effect of different magnetic fields, control samples with three replications were placed in gape out of magnetic field for 30 min. Results of study showed that the germination of seeds was significantly influenced by different magnetic fields depending on the days after treatment application. The first germinated seeds were observed two days after beginning of test in DC magnetic field (0.7 seed) treatment. In the last days of experiments (day ninth), no difference was observed among treatments. The growth behavior of seedling of cucumber affected by different magnetic field in comparison with control plants in greenhouse showed that seeds in which treated with AC magnetic field have better growth rate. The results of evaluation of plants in the field showed that parameters like fruit length (FL), the diameter of fruit (FMD), Fruit weight (FW), number of side stem (NSS), flower number (NF) and the number of flower per main stem were not significantly different at different times of experiment. Contrary to that, parameters such as the number of fruits per plant (NF) and the length of main stem (LMS) were significantly increased by time. Based on the obtained results of the germination, greenhouse and field trials it can be concluded that direct magnetic field stimulates seed germination and increases the growth rate and vigor of seedling especially at the beginning of germination. Generally, it can be indicated that the initial effect of magnetic field on the germination rate and the growth of seedling is very positive since it induces an improved capacity for nutrient and water uptake, providing greater physical support to the developing shoot
Molecular Characterization of Nepali Potato Cultivars using Randomly Amplified Polymorphic DNA (RAPD) Markers
Get PDFRandomly amplified polymorphic DNA (RAPD) was used to study the genetic diversity of four local cultivars of potato. Amplification with ten arbitrary decamer primers produced 29 different marker bands of which 69.0% were polymorphic. The size range of the amplified DNAs ranged between 370 bp and 2500 bp. On average, 17.5 alleles per genotype were amplified using the RAPD primers. With the selected primers sufficient polymorphism could be detected to allow identification of individual genotypes. A dendrogram displaying the relative genetic similarities between the genotypes showed a range of 55.2-69.0% similarity