Journal of Applied and Natural Science
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Standardized method to extract phenolic compounds from Lagerstroemia speciosa L. (Jarul) for enhanced antioxidant activity
Get PDFPhenolic compounds contribute to the antioxidant property of plants and the efficient extraction of plant phenolics could enhance its antioxidant potential. Lagerstroemia speciosa (L.) Pers. has been investigated for its pharmacological activities, but comparing its antioxidant activities of phenolics derived from its various parts has a key role in developing natural antioxidants. This study was conducted to standardize the extraction of phenolics from leaves, pods and branches of L. speciosa plant, followed by determination of antioxidant activities of their solvent fractions. Phenolic compounds were extracted from the leaves, pods and branches under different parameters such as temperature, pH, type of solvent and volume of the solvent. The extracted phenolic compounds were subjected to solvent fractions and antioxidant assays were performed. Among the various extraction methods tested, the best method was 50% ethanol +1% HCl, refluxing temperature, 100 ml of solvent and 1 hour extraction time. The best solvent fractions were determined as NaHCO3 + ethyl acetate for pods and ethyl acetate alone for leaves and branches with extracted phenolics content of 150.2 mg/g, 136.2 mg/g and 82.9 mg/g, respectively. The chloroform fraction was best among the fractions with maximum ascorbic acid equivalent (ASE) in all the parts of L. speciosa tested. Ferrous ion chelating capacity indicated that butanol fraction had the highest chelation and the same was recorded in ferric ion chelating assay with an EC50 value of 28.2. Hydroxyl radical scavenging activity of the fractions indicated that NaHCO3 + EtOAc fraction of pods had potential activity. Thus, the phenolic compounds from L. speciosa are excellent sources for future investigation on potent natural antioxidant compounds
Effect of soil and crop management practices on sodicity stress alleviation and rice productivity under water scarce condition
Get PDFThe prominent issue faced by the farmers of Cauvery Delta regionin Tamil Nadu particularly Tiruchirapalli District is the lack of timely release of water for rice nursery preparation and transplanting. Hence wet seeding of rice is recommended for timely cultivation. On the other hand, sodic nature of the soil warrants rice cultivation only. Therefore, the present investigation was carried out to study the different soil and crop management practices on alleviating sodicity stress and improving rice (variety ADT 3) productivity under water-scarce conditions. The experiment was laid in a split plot design with six mainplots,including rice wet seeding, daincha (Sesbania aculeata) application as green manure, anti-oxidant microbial consortia (AOMC) spray and four sub-plots with graded levels of NPK based on soil test values with three replication. Results showed that the daincha incorporation @ 6.25 t/ha followed by rice wet seeding + AOMC spray @1.5 % with 125 % soil test based NPK had significantly increased thechlorophyll content, SPAD values, proline content and grain and straw yields which remained on par with daincha incorporation @ 6.25 t/ha followed by rice wet seeding + AOMC spray @1.5 % with 100 % soil test based NPK.Significantly lower ESP at 5% level and higher phosphatase activity in soil was also recorded by daincha incorporation @ 6.25 t/ha followed by rice wet seeding + AOMC spray @1.5 % with 125 % and 100 % soil test based NPK. Gross return, net return and B:C ratio were also higher in the plot, which received daincha incorporation @6.25 t/ha followed by rice wet seeding + AOMC spray @1.5 % with 100 % soil test based NPK. The present study reveals that the inclusion of ectophytic microbial population spray in rice plants and the management practices helps the crop to tolerate the sodicity stress under water-scarce condition by maintaining required physiological functions like proline synthesis and enzyme activities etc which need to be further explored at the genotypic level.
Sorption of Arsenic(III) from wastewater using Prosopis spicigera L. wood (PsLw) carbon-polyaniline composite
Get PDFWater contamination by toxic heavy metal ions causes a serious public health problem for humans. The present work reports the development of a new adsorbent of PsLw carbon-polyaniline composite by direct oxidation polymerisation of aniline with PsLw carbon for the removal of arsenic (As). The structure and morphologies of the adsorbent were characterised by Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). The ability of the adsorbent for the removal of As(III) was estimated by batch and kinetic studies. The optimum adsorption behaviour of the adsorbent was measured at pH=6.0. The equilibrium process was found to be in good agreement with Langmuir adsorption isotherm and the maximum adsorption capacity was 98.8 mg/g for an initial concentration of 60 mg/L at 30 °C. The kinetic study followed pseudo-second-order kinetics. Thermodynamic parameters predict the spontaneous, feasible and exothermic nature of adsorption. Column operation was carried out to remove As(III) bulk and column data obeys the Thomas model. The results indicated that PsLw carbon-polyaniline composite can be employed as an efficient adsorbent than polyaniline for removal of As(III) from wastewater
Toxicity of diatomaceous earth on seed weevil, Sitophilus oryzae L. and its effect on agro-morphological characters of maize seeds
Get PDFSitophilus oryzae L. (Curculionidae; Coleoptera) is considered to be a serious internal feeder of stored cereals. The use of insecticides results in the development of resistance among the pests and residues in the produce. Diatomaceous Earth (DE) is from a natural source, environment-friendly, safe to humans and natural enemies. In addition, it is highly effective against a wide range of stored pest species and has no toxic residues on the treated seeds. The promising alternative to synthetic insecticides is the application of DE in storage pest management under physical control. With this background, the present study was aimed to find the efficacy of DE against rice weevil, S. oryzae L. and their effect on the agro-morphological characters of maize (Zea mays L.) seeds. Contact toxicity bioassays were carried out with different concentrations of DE against S. oryzae. The results of the bioassay studies revealed LD50 at the concentration of 1.27 mg/100 gm of maize seeds. Further, 100 per cent mortality was achieved at the dose of 15 mg/100 gm of maize seeds within six days of exposure. The effect of DE on the germination provided a significant increase in germinability of maize seeds (LD50= 94%, LD95= 98% and control= 96%). DE at the concentration of LD95 had a beneficial effect on the seedling parameters, especially germination% (98%) and seedling length (53.02 cm) of maize. The present study concluded that DE could be effectively utilised as an alternative management tool to chemical insecticides in the management of rice weevil under storage conditions
Multifunctional activity of graphene oxide-based nanoformulation against the disease vector, Aedes aegypti
Get PDFAedes aegypti management is a global concern due to the absence of medication and effective vaccines. The pesticide-mediated health hazards and rising insecticide resistance in mosquitoes have aggravated the issues. As graphene Oxide (GO)- based nanoformulations are considered a novel mosquito management strategy; the present investigation evaluated the efficacy of GO-based nanoformulations conjugated with malathion (ML) and endosulphan (EN) against Ae. aegypti. The GO was synthesised by Hummers’ method and was confirmed by UV-visible spectral analysis. The GO-ML and GO-EN binary mixtures (1:1 and 1:2) were assayed for toxic potential against mosquito larvae as per WHO protocol and the dead larvae were scrutinized for morphological deformations/abnormalities. The contact irritancy potential of GO nanoformulations was also evaluated against adult Ae. aegypti. The UV-visible spectrum of GO showed a narrow and high peak at ~300 nm corresponding to an n-π* plasmon peak. The GO-insecticide binary mixtures augmented the ML and EN toxicity by 80.43% and 6.43-fold, respectively. The GO-ML mixture-exposed larvae revealed cuticular deposition of black soot while larvae exposed to GO-EN exhibited disintegrated gut viscera. GO-insecticide combinations increased flights in Ae. aegypti denoting irritant potential. The effectual toxic, abrasive and irritant activity of GO-insecticide nanoformulations recommends developing graphene-based toxicants for mosquito managemen
Effect of different levels and sources of calcium on post harvest physiology of hybrid tomatoes (Solanum lycopersicum)
Get PDFDue to high respiration and ethylene production, tomato (Solanum lycopersicum) is highly perishable horticulture produce that deteriorates faster during the post-harvest storage period. Calcium nutrition is important to overcome these losses as it helps in retarding ripening process during storage and improve the post-harvest quality tomatoes. Hence a field experiment was conducted with different calcium sources and levels viz., Calcium sulphate, Calcium Nitrate, Calcium silicate, Poultry manure and Pressmud applied at 0, 20, 40, 60 and 80 kg Ca ha-1. A storage study was conducted in a factorial completely randomized block design (FCRD) to ensure the effective role of calcium in post-harvest quality of hybrid tomato. Tomatoes were analyzed for pH of fruit juice, firmness, titratable acidity, total soluble solids, moisture content and physiological loss at five days intervals upto 15 days. The results revealed thatCalcium sources and levels had highly significant impacts on the quality of tomato fruits. The tomatoes harvested from the plot applied with poultry manure at 80 kg Ca ha-1 retained the highest fruit firmness(3.76 N m-1), moisture content (88.2%) and the lowest weight loss (8.82%) in fruit, highertitratable acidity (0.41%), lesser pH (3.61)andtotal soluble solids (4.23 ˚Brix). Hence the application of poultry manure at 80 kg Ca ha-1was beneficial in improving the quality and extending the storage life of hybrid tomatoes
Evaluation of different traps for the invasive leaf miner, Liriomyza huidobrensis in potato (Solanum tuberosum) fields of the Nilgiris district, Tamil Nadu, India
Get PDFPotatoes are currently threatened by the pea leaf miner (Liriomyza huidobrensis Blanchard), an exotic, exceedingly polyphagous, and chemically resistant pest that attacks a wide range of crops, ornamental plants, and weeds. The present work was conducted to study the attractiveness of various traps to Liriomyza leaf miner, one of the invasive pests recently observed in potato fields of Kotagiri and Ooty in Nilgiris district of Tamil Nadu. A trial was conducted at these two different locations, the Nilgiris district, in 2021. The results revealed that among different traps tested for their efficacy in attracting the leaf miner, L. huidobrensis, yellow sticky trap was found to be more efficient in attracting adult leaf miner flies with the mean trap catches of 40.49 and 36.64 adult flies/ 10 cm2 areas at Kotagiri and Ooty respectively. The peak population of leaf miners was recorded in the last week of April (45.67 adult flies/ 10 cm2 areas) at Ooty and during the 3rd week of June (52.33 adult flies/ 10 cm2 areas) at Kotagiri. The correlation study revealed a significant positive correlation of the trap catches with maximum temperature, diurnal variation (DV) and growing day degrees (GDD). Multiple regression equation was also developed, where the abiotic factors contributed 46.1% and 65.5% to the Liriomyza leaf miner population fluctuation in the potato ecosystem. The trappers may be used to determine the initial presence of a leaf miners’ population and in projecting their future population through pest management models and management decisions
Crosstalk between circadian rhythms, sleep and eating habits to improve public health
Get PDFLifestyle changes in the past few decades have resulted in irregularity in sleep and meal timings. People are taking less sleep and resorting more to unhealthy food and eating habits. These factors are the most important health determinants. Sleep, food choices and eating habits are closely associated with each other. Thus, the crosstalk between circadian rhythms, sleep, and food needs urgent attention and discussion for better health management. Various research studies indicate that a healthy trend in sleep augments the regularity in meal timings and good eating habits. Healthy food and eating habits, in turn, boost sleep quality. Deterioration in the quality of anyone adversely affects the other. Several health disorders like obesity, diabetes, cancer, cardiovascular problems, and low immunity can be linked to these disturbances. The present review considers several previous studies to point out the inter-relationship between sleep and eating habits and the various health disorders arising from poor attention. Poor sleep promotes the tendency to eat junk food or eat at odd times, such as snacking late at night, leading to weight gain and other health problems. Junk food and unhealthy eating habits cause sleep disturbances. This inter-relationship of sleep and food preferences is important as it can have important treatment implications for health disorders arising due to these modifiable behavioural factors. Circadian alignment, improvements in sleep timings, and healthy eating habits have positive effects on other health behaviours as well.
Optimization with genetic algorithms of chlorogenic acid\u27s extraction and purification from native potato (Solanum tuberosum L.) peel, using an aqueous two-phase system
Get PDFChlorogenic acid is one of the main phenolic components of potatoes (Solanum tuberosum L.). Researchers have demonstrated that this phenolic compound is a higher concentration in the native Huagalina potato peel and is also found in the whole tuber\u27s cooking water. This research aims to extract and purify chlorogenic acid (CGA) from potato Huagalina peel obtained from discarded manufacturing for snacks, using an Aqueous Two-Phase System (ATPS). The central composite design rotatable (CDDR); Response Surface Methods (RSM) in R x 64 4.0.3 and RSM package was used to identify the regions of interest that resulted in the best extraction levels. The concentration of ethanol (EtOH) and disodium phosphate (DSP) were selected as factors capable of affecting CGA performance. Extraction efficiency values for concentrations were optimized using genetic algorithms (GA) applying the R package GA. . In the present research, a high value of 0.8 was applied, which produced new "offspring" solutions, which share some good characteristics taken from both parents. The mutation is applied after the crossover, altering some genes in the chains, which was 0.1. According to what has been reported, the offspring can replace the entire population or replace individuals with less aptitude. This evaluation cycle and selection-reproduction cycle were repeated until a satisfactory recovery of CGA (97.2%) estimated with GA at a pH of 3.4 (25ºC) was achieved, yielding 443.7 ± 0.062 mg CGA / 100 g dry weight of potato peels.
Residual effect of organics and direct effect of mineral nitrogen on rice (Oryza sativa L.) in two different textural soils
Get PDFNitrogen through organics and inorganics application is an alternate to maintain soil health and crop productivity in the rice cropping system. With this background, field experiments were conducted in farmer’s field at Kuttalam during 2012-2013, 2013-14 to evaluate residual organics and mineral nitrogen (100%N) on growth parameters and yield of rice (Oryza sativa) in sandy clay loam and clay loam soil. The treatments consisted of residual organics viz., composted coir pith (CCP), green manures (GM), sugarcane trash compost (STC), vermicompost (VC), poultry manure (PM) and FYM applied(100%N) and a combination of above residual organics with urea@50%N besides 100% recommended dose of nitrogen (RDN) and control. The results revealed that residual organics and urea recorded higher growth parameters like plant height, chlorophyll content, leaf area index(LAI), crop growth rate (CGR), relative growth rate(RGR), net assimilation rate(NAR), No. of tillers/hill compared to their individual addition. The growth parameters were more under residual PM in combination with mineral nitrogen in both the soils. Residual PM + fertilizer nitrogen (100% N) recorded highest grain (4485, 4693 kg ha-1) and straw yield (6984, 5897 kg ha-1) in clay loam and sandy clay loam soils, respectively. The lowest grain(3292, 2993 kg ha-1) and straw yield(4998, 4003 kg ha-1) were recorded in residual GM + fertilizer (100% N) in both soils. Among organics alone, residual PM(100% N) registered highest grain yield (4025, 4048 kg ha-1) in both soils. It can be concluded that the application of poultry manure alone or in combination with N fertilizers enhanced the growth and improved soil health.