Journal of Applied and Natural Science
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Seasonal variation in bacterial contamination of water sources with antibiotic resistant faecal coliforms in relation to pollution
Water sample were collected from piped supplies, surface water and ground water sources in different locations of Lucknow city during summer, monsoon and winter season. Bacteriological quality of samples was determined by enumerating coliform isolated were subject to antibiotic susceptibility test with disc diffusion method. Maximum coliform and faecal coliform contamination were recorded during summer (67% and 75%) and monsoon (67% and 58.3%) while minimum during winter (50% and 50%). All the test isolates exhibited resistance (for nine antibiotics) was shown by river isolates. Antibiotic resistance index (ARI) ranged from 0.050 to 0.150 exhibiting the broad spectrum resistance for 3 to 9 out of 10 antibiotics tested. Occurrence of faecal pollution indicator organisms and multiple antibiotics resistance bacterial population in drinking water is alarming and a sign of potential health with therapeutic problems
Role of arbuscular mycorrhizal fungi (AMF) in global sustainable development
Mycorrhizal symbiosis is a highly evolved mutually beneficial relationship that exists between Arbuscular Mycorrhizal Fungi (AMF) and most of the vascular plants. The majority of the terrestrial plants form association with Vesicular Arbuscular Mycorrhiza (VAM) or Arbuscular Mycorrhizal fungi (AMF). This symbiosis confers benefits directly to the host plant’s growth and development through the acquisition of Phosphorus (P) and other mineral nutrients from the soil by the AMF. In addition, their function ranges from stress alleviation to bioremediation in soils polluted with heavy metals. They may also enhance the protection of plants against pathogens and increases the plant diversity. This is achieved by the growth of AMF mycelium within the host root (intra radical) and out into the soil (extra radical) beyond. Proper management of Arbuscular Mycorrhizal fungi has the potential to improve the profitability and sustainability of agricultural systems. In this review article, the discussion is restricted to the mycorrhizal benefits and their role in sustainable development
X-ray structure analysis of 3-chloro-7-hydroxy-4- methyl-chroman-2-one
The title compound crystallizes in the monoclinic space group P21/c, with unit cell parameters a=7.7203(3), b=14.0481(4), c=8.9066(3) Å, â=112.858(5)º, V= 890.11(5) Å3 and Z = 4. The structure has been solved by direct methods and the final R-factor is 0.0433 for 2832 unique reflections. The molecule, as such, is planar and the planarity is confirmed by the magnitude of dihedral angles between the two rings. Extensive Hydrogen-bonding has been observed and chlorine atom is responsible for the formation of trifurcated hydrogen-bond. Trifurcated hydrogen-bond plays a significant role in the design and synthesis of molecules having drug implications
Infection status of Clinostomum complanatum (Rudolphi, 1819) metacercaria from Channa punctatus of Meerut District
Metacercaria Clinostomum complanatum is a digenetic trematode, which is mainly found in fresh water fishes. Present communication deals with the infection status of C. complanatum in C. punctatus of Meerut district, which is supported by the data spreading over one year. About 250 specimens of C. punctatus from different ponds of Meerut were studied through regular periodical collection in the year Jan 2010 to Dec 2010. Overall prevalence 35.6%, mean intensity 3.06 and abundance 1.09 were reported. The infection was maximum in winter and minimum in rainy season. Prevalence, intensity and abundance of the infestation were also found to be related to different length group and sex of the hosts, the medium sized fishes were more infected and the larger size fishes were less infected while the smaller size fishes showed moderate infection. Susceptibility of infection was not significantly different between male and female fish
Antibacterial and antioxidant activity of methanolic extract of bark of Prunus persica
In the present study in vitro antimicrobial and antioxidant activities of Prunus persica family rosaceae were carried out from the bark extract of the plant. The methanolic extract was tested for their antimicrobial study against gram positive and gram negative bacteria and for their antioxidant activity using scavenging activity of DPPH (1,1 diphenyl-2-picrylhydrozyl) radical method. The plant extract showed remarkable antibacterial and antioxidant activity
Influence of silica intoxication on female reproductive organs with therapeutic agents vitamin E and vitamin C
The aim of the present study was to observe the alteration of blood biochemical and histopathological variables after silica exposure, and also to show whether therapeutic agents (Vitamin E + Vitamin C) used in study may provide recovery against exposure to silica. In this study, silica was administered as silicon dioxide at a dose of 40 mg/kg for 28 days to produce toxic effect. Recovery pattern was evaluated by Vitamin E + vitamin C (50 mg/kg, ip + 100 mg/kg, po), The present study showed alterations in the activities of oestrogen and LH were significantly decreased in experimental rats after 28 days of silica intoxication. The level of progesteron and FSH, were also decreased. Therapeutic agents i.e. vitamin E and vitamin C recouped the values to normal and near to control
Phyto-availability of chromium to fluted pumpkin (Telfairia occidentalis Hook F.) in an ultisol
The present study was conducted to determine the influence of Cr on some agronomic characters of Telfairia occidentalis nutrient content and uptake and some chemical properties of soil. In the greenhouse trial, chromium nitrate [Cr(N03)2] was applied at rates of 0, 50, 100 and 200 mg per 5 kg sieved and air-dried soil obtained from a depth of 0-15cm. The rates of 0, 20, 40 and 80 kgha-1 equivalent to pot rates were used in the field trial. Results showed that the soil used was texturally sandy loam and an ultisol as revealed by its low base saturation. In the greenhouse the soil pH, N, K, Mg, Ca, Na, Fe, Mn, Zn, free Fe and Al oxides, organic carbon, effective cation exchange capacity, exchangeable acidity, amorphous Fe and Al oxides content of the soil decreased inconsistently at various levels of Cr treatments except available P, which appreciated inconsistently. With the exception of soil pH, organic carbon, available P and amorphous Fe oxide, which increased at various levels of Cr concentrations, all other soil chemical properties determined, declined inconsistently in the field trial. The amorphousAl oxide however remained stable in the field trial. The Cr content of the soil increased with the levels of Cr treatments when compared with the control in the trials. The N, P, K, Mg, Ca, Na, Fe, Mn and Zn content of shoot and root as well as their uptake also decreased consistently with increasing Cr treatments. In addition, the Cr content as well as uptake by the shoot and root also increased consistently with increased rates of the Cr applied in the trials with the minimum levels of the Cr content and uptake recorded at the control treatments. As the Cr concentration increased, the crude protein content of both shoot and root consistently decreased with highest crude protein content recorded in the shoot compared to the root. A decrease in the dry matter yield with increased Cr treatments in shoot and root was recorded in the trials. Results also showed that the Cr influenced the height, collar girth, leaf area and number of leaves with control treatments higher than other treatments at final harvest
Evaluation of genotoxicity of acetamiprid using PCR technique on mosquito genome
The present studies deal with the evaluation of the genotoxic potential of acetamiprid at LD40 on a mosquito Culex quinquefasciatus by adopting polymerase chain reaction technique (PCR). This technique was used for detecting DNA damage by amplifying ribosomal DNA internal transcribed spacer 2 (ITS 2) regions. The amplified products were sequenced and the results of treated and non-treated controls were compared using Clustal W software programme. The results were studied in the form of deletions, additions, transitions and transversions of the bases. The DNA band amplified from control stocks consisted of 444 bases while those from LD40 treated individuals were comprised of 448 bases. The total number of mutations caused in the treated stock was 230 out of which 84 were transitions, 117 transversions, 13 deletions and 16 additions. Thus, it was evident that acetamiprid has a potential to promote gene mutations in the individuals exposed to its semilethal doses
Impact of combined action of Neem and Eucalyptus oil volatiles on different stages of Corcyra cephalonica (Lepidoptera: Pyralidae)
Combined action of neem (Azadiracta indica) and eucalyptus (Eucalyptus sp.) oil volatiles causes a sharp reduction in percent egg hatchability in rice moth, Corcyra cephalonica (Stainton) (Lepidoptera: pyralidae) when freshly laid eggs were exposed to these volatiles for 24 hours. A marked decline in egg output and egg hatchability in reproductive pairs, was observed whose larvae were allowed to develop in a programmed manner in an environment, laden with combined action of selected volumes of neem and eucalyptus oil or when both the sexes of these pyralids, were confined for the prescribed limited period, in such odorous environment. A significant reduction in glycogen, lipid and protein level and an increase in free amino acids was noticed in testes and ovaries of these pyralids, when breeding pairs were exposed to the selected volatiles for a period of six hours only
Influence of Pongamia, Mahua and Neem cakes on finger millet productivity and soil fertility
A field experiment conducted at Bio-fuel park, Agricultural Research Station, Madenur, Hassan in Kharif season of 2009 to asses the performance of finger millet (Eleusine coracana L.) under different organic manure treatment consisting of four treatments viz., recommended FYM and NPK through inorganic fertilizers as control, Pongamia, Mahua and Neem cake with 5 replications laid in randomized complete block design. The results revealed that application of recommended FYM along with neem cake equivalent to 100% recommended N performedbetter in respect of finger millet productivity and maintenance of soil fertility followed by recommended FYM with 100% NPK through fertilizers. Nutrient supplementation with different oilcakes proved superior in respect of soil sustainability