Journal of Applied and Natural Science
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Studies on the toxicity of 2-Methyltetrahydrofuran on the histopathology of gills of African catfish Clarias gariepinus
Get PDFIn the present study, investigation were carried out on gills of African cat fish Clarias gariepinus exposed to sub lethal concentrations (80mg/ml,400mg/ml and 800mg/ml) of 2-Methyltetrahydrofuran for 10 days. Lesions were observed in gills tissue of treated fish for long term exposure to Methyltetrahydrofuran (2MTHF). The occurrence and degree of alteration were positively related with the concentration of 2MTHF. Histological examination of the gills of fish treated with 80 mg/ml of 2MTHF for 10 days showed architectural loss, necrosis and desquamation of epithelial layer. Histological examination of the gills of fish treated with 400 mg/ml of 2MTHF for 10 days showed architectural loss, necrosis and mild vaccuolation. The gill filament exhibited telangiectesis, disorganisation of secondary gill lamellae and complete vaccuolation of gills treated with 800 mg/ml of 2MTHF for 10 days. The study indicated that 2MTHF had marked effects on the cyto-architecture of the gills of C. gariepinus. The degree of vaccuolation and necrosis were positively related with the concentration of 2MTHF
Role of sugarcane bagasse and bamboo for adsorption of hydrolysed dyes from textile effluent: An overview
Get PDFAdsorption process is one of the best ways for removal of dyes from effluent generated from different industries. The use of adsorbent manufactured from sugarcane bagasse as well as bamboo fibre bundles, an agro squander from sugar and building industries have been reviewed as an excellent replacement for stimulated carbon sorbents for the removal of dyes from wastewater. Agricultural based adsorbents prepared from sugarcane bagasse and bamboo may be successfully used to remove the unused hydrolyzed dyes from an effluent of textile dyeing industry. These ready adsorbents may very much capable in decolorization of the textile effluent. In this review, literature of two sorbents has been compiled. The review assesses these agricultural based materials as low-cost adsorbents for the removal of hydrolysed dyes from textile effluent. The review also draws some of the basic mechanism of dye adsorption on to. 
Intra-specific morphological and morphometric variability of Radopholus similis (Cobb, 1893) Thorne, 1949
Get PDFAll the root inhabiting migratory endoparasitic nematode populations of Radopholus procured from banana crop of Vellayani, Thiruvananthapuram, Kerala were identified as Radopholus similis. Heat killed females were straight to slightly ventrally curved posteriorly. Female’s head was low, rounded, continuous or slightly setoff with the body contour. Females were 500-660 µm long and were comparatively longer than males. Males had button shaped head set off by a constriction; female with three to five lip annuli, four crenate and areolated lateral incisures, stylet 14-18 µm long with rounded knobs, vulva post-equatorial (58%), sometimes with slight protuberant lips, ovary paired and equally developed, oesophageal gland overlapped the intestine dorsally, tail elongate-conoid with narrowly rounded terminus. The stylet length (µm), width of stylet knob (µm), distance of excretory pore from anterior end (µm), distance from head to basal bulb (µm), lateral field structure, shape of stylet knob, head region, position of phasmid, tail shape with its terminus, morphometric values like m%, o% and v% and a, c and c´ ratios of females were stable (CV<12%) features. There is an existence of intra-specific variability in the morphological and morphometric features of R. similis. The main morphological diversity was observed with P% of male and female, b ratio of female and stylet length, distance of DEGO from stylet base, o% and T% of male. All the root inhabiting migratory endoparasitic nematode populations of Radopholus Thorne, 1949 procured from banana of Vellayani, Thiruvananthapuram, Kerala were identified as Radopholus similis (Cobb, 1893) Thorne, 1949. A high degree of intra-specific morphometric variability was observed with regard to the total body length (µm), body width (µm), stylet length (µm), distance of dosrsal oesophageal gland orifice (DEGO) from stylet base (µm), number of lip annuli, lip height (µm), distance from head to basal bulb (µm), distance of anus from anterior end (µm), tail length (µm), anal body width (µm), distance of phasmid from tail terminus (µm), number of lateral lines, width of lateral field (µm), b ratio and P % among females of R. similis. Morphometric features like m%, o% and v% of females of R. similis showed least variability. These can be considered as the stable morphometric characters for discrimination of females of R. similis. Ratios like ‘a’ and ‘c’ of females of R. similis were found moderately variable. The morphometric feature and of male i.e. distance from head to basal bulb (µm) was found least variable; while number of lip annuli and spicule length (µm) were moderately variable.  
A study on socio- economic condition of Tharu tribes in Bahraich district of Uttar Pradesh in India
Get PDFAs per the census of 2011 the tribal population consists of 8.6% while it was 8.2% according to the census of 2001 in India. This paper addresses the socio-economic status of Tharu tribes in Bahraich district of Uttar Pradesh. The socio economic status is an important determinant of health, nutritional status, mortality and morbidity of an individual. Socio Economic Status also influences the accessibility, affordability, acceptability and actual utilization of available health facilities. To classify the socio-economic status of the respondents, Uday Pareek and Trivedi scale has been adopted with subject to preliminary test and slight modification as per the need of the study. Measuring the socio-economic conditions of Tharu tribes from Uday Pareek and Trivedi scale, it can be concluded that all the Tharu tribes belong to four categories viz: Upper Middle Class, Middle Class, Lower Middle Class and Lower Class. 0.28% respondent belonged to upper middle class, 5.33% were middle class, 75.56% belonged to lower middle class and 18.83% to lower class. The findings can be beneficial for implementing projects that can help in improving their situation of backwardness. 
Identification of microbial contaminants in drinking water samples of Ahmedabad using PCR
Get PDFQuality of drinking water is one of the greatest anxiety of water consumers with contamination of different pathogenic microorganisms especially fungi and bacteria. Water-borne pathogen contamination in water resources and related diseases are a major water quality concern throughout the world. A motivation for this study was the numerous reports about these contaminations of drinking water and the associated diseases. Water samples were collected from various locations of Ahmedabad in February 2017. The samples were analyzed by 16S rDNA PCR and ITS rDNA PCR since the DNA sequence polymorphisms in the 16S ribosomal DNA and ITS rDNA can be used for the identification of bacterial and fungal species respectively. The PCR amplicons were obtained for each water samples, were sequenced and the species identity was determined using NCBI-BLAST. Bacterial species of Aeromonas, Citrobacter and E. coli along with two fungal species were obtained from municipal water supplies and parab waters. Species of Aspergillus and Penicillium represented the most common fungi in municipal water supplies. This is the first report of molecular study from drinking water facilities of Ahmedabad. This study has clearly revealed that currently, the microbiological quality of the water is inferior for drinking purpose due to bacterial and fungal contamination. This study also showed the potential PCR based identification as a rapid technique for identifying microorganisms in drinking water samples
Assessment of water quality around coal-fired thermal power plant, Bathinda (Punjab), India
Get PDFIn the present study, both the underground and surface water samples around Guru Nanak Dev Thermal Power Plant Bathinda, Punjab, having total installed capacity of 440MW, were analyzed for the physico-chemical parameters viz. pH, Electrical Conductivity (EC), Total Dissolved Solid (TDS), Total Hardness (TH), Calcium (Ca2+), Magnesium (Mg2+), Total Alkalinity (TA), Carbonate (CO32-), Bicarbonate (HCO3-), Chloride (Cl-), Phosphate (PO43-), Nitrate (NO3-), Fluoride (F-) and heavy metals such as Cadmium (Cd), Copper (Cu), Lead(Pb), Nickel (Ni) and Zinc (Zn). The correlation coefficients (r) among various water quality parameters of studied underground water samples were calculated. The physical and chemical parameters of groundwater are compared with the drinking water quality standards recommended by Bureau of Indian Standards (BIS) (2012) and World Health Organization (WHO) (2017). On the basis of results obtained in the present study, concentration of Chloride (Cl-), Magnesium (Mg2+), Fluoride (F-) and Total dissolved solids (TDS) observed in ground water of Bathinda varied from 30 to 284 mg/l, 120 to 280 mg/l, 2.66-3.99 mg/l and 620-1439 mg/l, respectively which are exceeding the acceptable limits prescribed by WHO (2017) as well as BIS (2012). Also, the concentration of heavy metals viz. cadmium (0.366 to 0.437 mg/l), and lead (0.193 to 0.353 mg/l) have higher levels than prescribed by WHO and BIS for drinking purpose. Thus, groundwater is not suitable for drinking purposes with prior treatment
Phytotoxic impact of Parthenium hysterophorus L. on Macrotyloma uniflorum a pulse crop in a dry tropical environment, Bihar, India
Get PDFAn experiment was conducted to evaluate the effect of aqueous extract of leaf, stem and root of Parthenium hysterophorus on the rate of seed germination (%) and seedling growth (cm) in Macrotyloma uniflorum. The different concentrations of root, stem and leaf extract used in the experiment were 15, 25, 50, 75 and 100%. The rate of seed germination, length of root and shoot and Seed Vigour Index (SVI) values were recorded. Data collected were analysed for Pearsons Correlation Coefficient, Tukey HSD (Honestly Significant Difference) and Post HOC Tests. It was recorded that SVI value decreased in different concentrations of leaf, stem and root from 23.53 to 100%, 3.33 to 26.21% and 32.44 to 100%, respectively. This indicated that leaf and root extracts were more phytotoxic to M.uniflorum than the stem extract of P.hysterophorus. The rate of seed germination and length of shoot differed significantly in different concentrations than the length of root, as indicated by Post HOC Test. The effect of plant parts i.e. root, stem and leaf; treatments i.e. different concentrations and interaction of plant parts and treatments were significantly differed at p < 0.000. Generally the impacts of different parts were also significantly different at p < 0.000. Thus, this study indicated that the leaf and root extracts are more toxic to M. uniflorum than the stem extract; and the rate of seed germination and shoot length were more affected than the root length. The seed germination and shoot growth in M. uniflorum are affected by leaf and root extracts of Parthenium. 
Assessment of triasulfuron on weed dynamics and yield of transplanted rice (Oryza sativa L.)
Get PDFA field experiment was managed during kharif season of 2014 in G. B. Pant University of Agriculture and Technology, Pantnagar (Uttarakhand) to assess the outcome of applying dissimilar rates of triasulfuron on the transplanted rice (Oryza sativa L.) associated weeds. Treatments composed of triasulfuron at three different rates, metsulfuron methyl, ethoxysulfuron, 2, 4-D with 2 checks (weed free and untreated). Experiment was carried out in Randomised block Design with 8 treatments and 3 replications. Grassy weeds, broad leaf weeds and sedges were observed during the crop growth period. Triasulfuron at 12 g ha-1 proved most efficient in controlling weeds at 60 and 90 days after transplanting (DAT) with 44.4 % and 59.6 % WCE respectively. Minimum weed dry matter (65.1 g m2) was observed with triasulfuron at 12 g ha-1. Out of all the herbicidal treatments, triasulfuron at 12 g ha-1 gave the paramount grain yield of rice (5786 kg ha-1) which was significantly surpassing all other herbicidal treatments, while it was minimum in metsulfuron methyl at 4 g ha-1 (4417 kg ha-1)
Algae: A potential source for nanoparticle synthesis
Get PDFNanotechnology deals with the particles of diameter size less than 100 nm. Nanoparticles are different from their bulk counterparts due to their distinctive shapes and sizes. They provide larger surface area, essential to carry out different chemical reactions. Other than the physical and chemical methods of nanoparticle synthesis, biological method is getting more popularity, as the process is less toxic, cost effective and eco-friendly. Various biological sources such as algae, plants, bacteria, fungi etc. have been explored for their potential to synthesize nanoparticles. In the literature, algae was found to be more reliable source for the green synthesis of metal nanoparticles as they are readily available in nature and are easy to grow in normal conditions. Metal nanoparticles like gold, silver and iron synthesized from algal source have widespread application in treating environmental pollutants like heavy metal removal, degradation of organic dyes, antimicrobial agents etc
Study of indigenous freshwater fish diversity of Bankura (West Bengal), India with special reference to Clarias batrachus
Get PDFA study was conducted to determine the ichthyofaunal diversity in Bankura district of West Bengal. The area is traversed by major and minor rivers, feeder channels, numerous ponds, bills, reservoirs which have made this drought-trodden district as the highest producer of aquatic products within the state. Field survey was conducted in randomly selected local markets covering twenty-two blocks of Bankura. Fish sampling was carried out using various conventional fishing gears. The entire region harbours a wide variety of aquatic fishes. A total number of 92 indigenous fish species belonging to 30 families were identified during this study. The Cyprinidae family dominated the population with its 36 varieties followed by Channidae, Siluridae and Bagridae. Sonamukhi block situated in Shali basin; Sarenga and Raipur of Kangsabati basin; Dwarkeshwar and seven bundhs- enriched Bishnupur were the major habitats of small indigenous fishes. This paper also denotes presence of 12 globally endemic freshwater fish species viz. Hypophthalmichthys molitrix, Cyprinus carpio, Labeo nandina, Tor khudree, Chitala chitala, Bagarius bagarius, Wallago attu, Ompok pabda, Ailia coila, Anguilla bengalensis, Parambassis lala, Oreochromis mossambicus. The substitution of native Clarias batrachus with invasive African catfish C. gariepinus was observed in various local markets. Some traders even promote illicit farming of this banned species for their own profit. As a consequence, the indigenous, nutritionally-enriched C. batrachus is becoming more endangered