Journal of Applied and Natural Science
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Responses of the antioxidant defences of Labeo rohita exposed to Basic violet-1 (BV-1)
Get PDFPresent study envisaged evaluating the effect of Basic violet-1 (BV-1, CI No. 42535), a widely used azo dye in dyeing and textile industries, on antioxidant enzymes of Labeo rohita fingerlings (7.6 – 11.3 cm length and 16.1 – 26.7 g weight). Antioxidant enzymes such as acetylcholinesterase (AChE), succinate dehydrogenase (SDH), aspartate transaminase (AST) and alanine transaminase (ALT) were estimated in liver, kidney, gill, muscle and brain of the fish as markers for the stress of BV-1. After 96h, the fish were kept for a recovery period of 30 days and activity of enzymes was determined at 15 day intervals. Significant dose dependent increase over control in the activity of AST (79.69%) and ALT (50.07%) was observed in all the tissues while a significant decrease over control was observed in AChE (717.43%) and SDH (173.07%) activity in all the tissues. Alterations in the activity of enzymes could probably be due to a defense against oxidative damage caused by the dye and prolonged effect till the end of the recovery period could be a metabolic adaptation of the fish to the stress of the present dye. The results indicate that the dye is very toxic to L. rohita as there was a marked change in the activity of selected enzymes in the exposed fish and the effect prolonged till the end of recovery period. So these enzymes in the selected tissues can be considered as best biomarker to determine toxicity of even very low doses of the azo dye BV-1 in fish
Genetic diversity analysis in tossa jute (Corchorus olitorius L.) germplasm lines
Get PDFAn experiment was conducted to examine the magnitude of genetic diversity and characters contributing to genetic diversity among 81 tossa jute genotypes. Cluster analysis based on Euclidean squared distances and ward’s method, the genotypes were grouped into ten distinct clusters. Analysis reveals that stick weight contributes maximum to the phenotypic diversity (65.52%) followed by green weight (13.64%) and fibre yield (10.10%). Among the clusters Cluster IX recorded highest mean fibre yield (19.91g) followed by Cluster VII (18.94g) and these clusters also recorded high mean values for plant height, basal diameter, green weight and stick weight. The highest inter- cluster distance was 186.80 (between clusters II and X) followed by 161.26 (between clusters IV and X), indicating the wide genetic diversity among these clusters. The highest intra-cluster distance was observed in cluster II (20.34) and the lowest in cluster X (3.17). The average inter-cluster distances were higher than the average intra-cluster distances, which shows the presence of wide genetic diversity among the genotypes of different clusters than those of the same cluster. The first two principal components, whose Eigen values are greater than one, accounted for 74% of the total variation among the five characters. The information obtained from diversity analysis is useful in planning further breeding programme for tossa jute improvement
Seed yield of linseed varieties grown as ‘paira’ crop as influenced by dates of sowing
Get PDFLinseed is an industrial crop cultivated for its seeds, fibres and oil purpose. Linseed crop can met their requirement i.e. moisture and nutritional demand from stored soil moisture and residual fertility status in "utera? or "paira? system of cropping. On the basis of this fact an experiment was conducted on “Seed yield of linseed varieties grown as paira crop as influenced by dates of sowing” in red and laterite zone of West Bengal during rabi season of 2012-13 and 2013-14 at Rice Research Station, Bankura, West Bengal, India. Poor in organic matter content, available phosphate and bases, hard structure of iron and aluminium patterned as honeycomb are present in the subsurface regions of the profiles and kaolinite is the predominant clay minerals of red and lateritic zone (western part) of West Bengal. This experiment was laid out in a split-plot design with three replications and compared two factors (dates of sowing and linseed varieties). Objective was identifying the optimum date of sowing and suitable linseed varieties grown as „paira? crop under changed climate in red and laterite zone of West Bengal. The experimental results revealed that the highest seed yield (534 kg ha-1 as pooled value) was recorded from the treatment D1 i.e. linseed sown on 15th November. The lowest seed yield (489.2 kg ha-1 as pooled value) was obtained with treatment D3 i.e. linseed sown on 29th November. Among linseed varieties, T-397 has yielded highest seed yield (573.4 kg ha-1 as pooled value). Lowest seed yield (409. 3 kg ha-1 as pooled data) was recorded from Neela variety. From the present study it may be concluded that linseed sown on 15th November is the best time and T-397 is the suitable linseed variety grown as "paira? crop under changed climate in red and laterite zone of West Bengal
Losses and alternatives measures towards environmental degradation in rural area of Haryana
Get PDFIn India, the globally accepted threats caused by environmental degradation are soil degradation and desertification, pollution, droughts, floods and water scarcity. The study was conducted in two districts of Haryana state namely, Hisar and Kaithal and a total number of one hundred twenty women were selected and interviewed with the help of well structured schedule. The study revealed that majority of the women were of middle age group (36-50 years), educated up to higher education, belonged to nuclear family with 4 members, performed government, private jobs and self employment. Very serious losses due to environment degradation were air pollutant effect on human health, animals and plants (1.47), deeper level of ground water table leads to irrigation scarcity (1.35), quality deterioration of crop produce and fertilizers cause toxicity in food products (1.23), lost of biodiversity and tropical forest, extinction of plant and animal species (0.41) as per their ‘Z’ score. Least smoky trees should be planted and used, Baval, Neem, Pipal, Bargad, Shisham, Aam, Jamun (94.16%), Installing chimneys to rent smoke from indoor stoves (90.83%) Storage and disposal of farm wastes properly (79.16%), Campaigning for healthy environment (74.16%), enhancing the area under agro and social forestry (69.16), empowerment of women for different development programs (66.66%) were found very effective among the women to making the environment healthy. To reach the results the aggregate total score was calculated to each alternative measure separately, and on the basis of calculated total score, a weighted mean score were obtained which were ranked according to the maximum or minimum mean scores for assessing the effectiveness of alternative measure
Assessment of genetic divergence for quantitative traits in thermo tolerant tomato (Solanum lycopersicum L.) genotypes
Get PDFIn the present study, 21 thermo tolerant tomato (Solanum lycopersicum L.) genotypes were evaluated to delineate the extent of genetic diversity through 10 quantitative traits. The genotypes were categorized into 4 distinct clusters using D2 statistics. Cluster IV had the maximum number (10) of genotypes, where all heat tolerant genotypes grouped together, followed by cluster I (5). Intra cluster analysis revealed that the cluster IV had highest distance, while the inter cluster distance was maximum between cluster III and cluster IV (6.53) indicating the presence of wide range of variability among genotypes of the cluster. Cluster IV recorded maximum cluster mean for yield (579.0), average fruit weight (33.33) and fruit set per cent (54.8) and the cluster III recorded highest mean value for days to 50 percent flowering (67.33). Principal component analysis showed that the first two principal components (PC’s) accounting for 77.6 per cent of total variation. On the basis of inter cluster distance, cluster mean and principal component analysis observed in the present study, a crossing programme involving genotypes from cluster IV will be promising one for developing heat tolerant tomato hybrids
Performance evaluation of Border irrigation method for cotton field
Get PDFPerformance evaluation of Border irrigation method was carried out for cotton field in village Kirarkot, Sirsa (Haryana). Water application, storage and distribution efficiency were estimated using measurements of soil moisture (%), infiltration rate (cm/hr), water advance and recession time (minute) during different irrigation events. The advance time increased during the growing season due to increased infiltration rate and increased resistance to flow by the growing crops. The water application efficiency of cotton field was 100 per cent as average applied depth (8.26 cm for canal irrigation and 9.06 cm for tubewell irrigation) of irrigation was less than the average required depth (10.30 cm for canal irrigation and 10.98 cm for tubewell irrigation) throughout the field plots. The observed water storage efficiency in different cotton fields varied from 72.92 - 90.08 per cent indicating under irrigation. Water distribution efficiency of cotton fields (97.8 -99.2per cent) indicated a relatively high degree of uniformity of water application. Stratified soil profile (sandy loam: 0-30 cm and sandy clay loam: 30-120 cm) of the selected fields reduced the infiltration rate to relatively very low value after 10-15 minutes creating favourable condition for uniformity of water application under border irrigation
Effect of integrated nitrogen management on NPK uptake in basmati rice (Oryza sativa L.)
Get PDFA field experiment entitled NPK uptake influenced by integrated nitrogen management in basmati rice (Oryza sativa L.) was conducted at Punjab Agricultural University, Ludhiana during kharif 2010. The experiment was conducted in randomized block design with 13 treatment combinations of chemical fertilizer, green manuring (GM) with Crotalaria juncea, farmyard manure (FYM) and organic preparations [OP (Jeevamrit and Panchagavya)] in four replications. The highest grain yield (34.9±0.54 q ha-1 ) was obtained with combined application of FYM and 50 per cent of recommended nitrogen (RN) followed by GM+FYM+OP (33.7 q ha-1 ) and GM+FYM (33.4±0.99 q ha-1 ). Straw yield (63.1 q ha-1 ) increased significantly (P<0.01) in treatment where FYM combined with 50 per cent of RN was applied. Among different nitrogen management treatments, the maximum N, P and K uptake in grain and straw were observed under treatment T8 (FYM +50 per cent of RN) viz. integrated nitrogen treatment followed by T11 (GM+FYM+OP) and T9 (GM+FYM). Thus, combined application of FYM @ 19.76 t ha-1- with reduced fertilizer dose (50 % of RN) increased the yield and NPK uptake in basmati rice
A smart precision plant protection technique based upon information and communication technologies for field crops in India for wide-area implementation
Get PDFA smart and modern technique based upon information and communication technology (ICT) was conceptualized and developed. It facilitates pests’ information collection timely, its fast processing and planning with quickly implementable options - to manage biotic stresses on field crops on large-scale area basis. The pest mapping in field crops (paddy, cotton) in different agro-ecosystems of India was done based on using Geographic Information System (GIS) Arc-info package. To fast track the decision-making, its mass communication to farming community, an exhaustive e-database of plant protection measures as recommended by different agencies in the country for different field crops viz., cereal crops, oilseed crops, pulses, fibre crops, sugar crops, fodder crops etc. - had also been prepared and digitized. The outcome and benefits of this technique had been analyzed and findings - that demonstrate the benefits of using the proposed architecture are also detailed
Ultrastructural study on morphology of Schistosoma spindale by Scanning electron microscopy (SEM)
Get PDFThe present study was undertaken to investigate the detailed morphological features along with morphometry of different structures of Schistosoma spindale (Adult flukes) which were recovered by a perfusion technique and visualized by Scanning Electron Microscopy (SEM). The length of spines on the oral sucker and ventral suckers were 2.6 µm and 2.5 µm, respectively. The measured width of aspinose area beneath the ventral sucker, rim of the ventral sucker and tegumental papillae were 5.4, 22.5 µm and 3 µm, respectively. Males have a welldefined gynaecophoric canal, originating just below the ventral sucker and extending up to the posterior end of the body, continued as a marked conical projection. The ventral surface of the oral sucker was completely covered with numerous spines. The ventral sucker was pedunculated, round, thick-rimmed and the inner side contained numerously pointed spines directed towards the center of the ventral sucker. The tegument surface of S. spindale showed ridged layers with large uniciliated and pit like papillae which were recorded more in posterior end. Thus, Scanning Electron microscopy (SEM) provided indepth ultrastructural morphological details of Schistosoma spindale which was in accordance with that of previous studies, would be applicable for its differentiation with other species (S. mansoni, S. bovis, S. haematobium, S. japonicum)
Effect of rejuvenation pruning on the growth, productivity and disease incidence in declining trees of pomegranate (Punica granatum L.) cv. Kandhari Kabuli
Get PDFPruning of fruit trees is an important aspect for the improvement of fruit quality and to minimize the pests and disease attack. Pruning was performed in the month of December in seven year old unpruned trees of Punica granatum cv. Knadhdri Kabuli having many interfering branching and a heavy infestation of bacterial blight orchard. The pruning treatments viz., T1: Retention of 15cm fruiting shoot length, T2: Retention of 30cm fruiting shoot length, T3: Retention of 45cm fruiting shoot length, T4: Retention of 60cm fruiting shoot length and T5: control (No heading back and no thinning) plant. The results of the present investigation revealed that among different pruning treatment, the best results in terms of shoot extension (56.34 cm), fruit size (Diameter 9.66 cm and Length 9.65 cm, fruit weight (278.50 g), marketable yield (10.25 kg) and fruit qualities were in fruits from T1 and T2 where retention of 15cm fruiting shoot length respectively and retention of 30cm fruiting shoot length were maintained. However, maximum fruit set (54.73%) was recorded in control, and it decreased with increasing pruning intensity. The pruning treatments also proved beneficial in controlling bacterial blight on fruit (12.86%) and leaf surface (26.60%) to some extent