Ardabil University of Medical Sciences
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Investigation of the association between SNPs in the microRNAs binding site in theMTHFR gene and preterm labor in Ardabib province
Get PDFBackground and Objective: Preterm labor is the most common cause of neonatal mortality, while infant mortality rates are a criterion for assessing the quality and comparisons of health systems in communities. Evidence suggests that MTHFR polymorphism is currently involved in early delivery. In recent years, various studies have focused on the association of polymorphisms (SNPs) with the MTHFR metabolism process, and reports have been reported in SNP in various parts of the MTHFR gene. Regarding the importance of the subject, this study aimed to investigate the association between C677T and A1298C polymorphisms in the MTHFR gene with preterm delivery in Ardebil province.
Methods: In this case-control study, 50 pregnant women with preterm labor (less than 37 weeks) as case group and 50 pregnant women with a delivery of more than 37 weeks were selected as control group. A questionnaire containing demographic information and dietary intakes containing folic acid was used to evaluate the daily folic acid intake. This information was analyzed using the Processor Food (FP) software. From each individual, 2.5 cc of blood was collected in EDTA tubes and sent to the Genetic Laboratory for analysis. Based on the results, the relationship between the polymorphism in the MRRC region of the MTHER gene in preterm labor was studied in both control and control groups. inclusion criteria included Nolly para pregnant women lacking any specific disease that experienced early labor symptoms and did not have any contractional factors such as preeclampsia or gestational diabetes mellitus, genital infections and bleeding in the first trimester, and mothers With specific and multiple illnesses, dissatisfaction with sampling, the occurrence of any medical condition, including fear Ray cardiovascular, hypertension, liver disease, kidney disease, thyroid disorders, infectious diseases, diabetes and taking any medication except folic acid, multivitamins were excluded.
Results: In this study, the mean age of the patients in the case group was 26.96 ± 6.43 in the control group was 23.60 ± 5.35 years. The prevalence of C677T polymorphism among women with preterm delivery and control was 50% and 60%respectily. Forty-four percent of women with preterm labor and 60% of control group were heterozygote for A1298C polymorphism and 50% of women with preterm delivery were homozygous for this polymorphism, while in control group 40% of the homozygotes were observed. The prevalence of A1298C polymorphism among women with preterm delivery and control was 80% and 70%respectily. 66% of women with preterm delivery and 70% of control group were polymorphisms of A1298C gene heterozygote and 14% of women with preterm delivery were homozygous for this polymorphism, while in the control group women did not have a consensus.There was a significant relationship between C677T and A1298C polymorphisms of MTHFR gene with preterm labor.
Conclusions: In this study, there was a significant relationship between C677T and A1298C polymorphisms of MTHFR gene with preterm labor, which can be considered as a major risk factor for preterm labor
Effect of ceftriaxone on paired-pulse response and long-term potentiation of hippocampal dentate gyrus neurons in rats with Alzheimer-like disease
No full textGlutamatergic dysfunction is posed as a main stage in neurodegenerative disorders such as Alzheimer's disease (AD). Glutamate-mediated excitotoxicity contributes to cognitive dysfunction and cell death in AD. Ceftriaxone (CFT), a well-known upregulator of GLT-1, selectively induces the expression of glutamate transporter-1 (GLT-1) in different brain regions and therefore can be posed as a potential candidate for elimination of glutamate-induced excitotoxicity which is an early prominent event in AD brains. This study was designed to investigate the electrophysiological and behavioral effects of the β-lactam antibiotic ceftriaxone in okadaic acid (OKA)-induced model of AD.
MATERIALS AND METHODS:
Male Wistar rats divided into four control, ceftriaxone (CFT), OKA, and OKA plus ceftriaxone (OKA + CFT) groups. OKA was injected intracerebroventricularly (i.c.v., 200 ng/5 μl) into lateral ventricles and after two weeks the evoked field potential recorded from hippocampal perforant path-DG synapses in order to evaluate the effect of ceftriaxone treatment (200 mg/kg/day, i.p.) on long-term potentiation (LTP) and paired-pulse responses.
KEY FINDINGS:
Results of this study revealed that ceftriaxone treatment significantly ameliorates the OKA-induced attenuation of field excitatory post-synaptic potential (fEPSP) slope and population spike (PS) amplitude following high-frequency stimulation and paired-pulse paradigm indicating its beneficial effects on both short-term and long-term plasticity in these neurons. Ceftriaxone also has an improving effect on OKA-induced impairment in short- and long-term memories evaluated by alternation behavior and passive avoidance tasks in rats.
SIGNIFICANCE:
Therefore, this study suggests that GLT-1 might be a promising therapeutic target for treatment of neurodegenerative disorders such as AD in the future
Decreased expression of the Keap1gene and its clinicopathological significance in gastric cancer: correlation with promoter DNA methylation
No full textBACKGROUND: Kelch–like ECH–associated protein1 (Keap1) is a cytoplasmic anchor of nuclear factor erythroid 2–related factor2 (Nrf2), which mediates the regulation of genes involved in apoptosis and cell survival. Epigenetic events such as aberrant DNA methylation is emerging as a possible mechanism for dysregulation of the Keap1 in cancer. However, the impacts of Keap1 methylation and expression on gastric cancer(GC) development and progression have not yet been investigated. METHODS: We analyzed the Keap1 promoter methylation and its mRNA or protein expression in endoscopic biopsies from 60 GC patients compared with those in non–GC individuals by methylated DNA immunoprecipitation(MeDIP) assay, quantitative real–time PCR and immunohistochemistry, respectively. RESULTS: The methylation level of Keap1 in GC was remarkably higher than that in non–GC biopsies (p<0.001). In contrast, the mRNA and protein expression of Keap1 were clearly down–regulated in GC in comparison with the non–GC tissues (p<0.001). In addition, there were significant differences in both Keap1 methylation or expression levels between the low and high histological grade. Statistical analysis revealed that Keap1 expression inversely correlates to its promoter methylation in GC (r =–0.55, p<0.001). CONCLUSIONS: Our results reflect an epigenetic regulation of Keap1 expression, and highlight the importance of Keap1 downregulation and its promoter hypermethylation in GC pathogenesis
THE EFFECT OF CONJUGATED LINOLEIC ACID ON THE SERUM LEVEL OF LEPTIN IN COPD PATIENTS
Get PDFPURPOSE:Chronic Obstructive Pulmonary Disease (COPD) accompanied by systemic inflammations and is characterized byirreversible airflow limitations. Leptin is a cytokine with pre-inflammatory effect. However, there are no studies investigating theeffect of Conjugated Linoleic Acid (CLA) on the serum leptin in COPD patients. Therefore, the present study aimed to explore theeffect of CLA on the serum leptin in COPD patients.METHODS:This interventional study was conducted on 90 COPD patients. The patients randomly were divided into two groups(supplement and placebo), each consisting of 45 patients. After obtaining written consent from the patients and recording theirdemographic characteristics, the spirometry was conducted and CAT score was calculated. Moreover, blood sample was collectedfrom each of themin order to analyze their serum leptin. After that, the patients in the supplement group were administered with3.2 g/day of CLA for 6 weeks. The patients in the other group received placebo. After the intervention, spirometry, CAT scorecalculation and blood sampling were conducted once againfor all of the patients and the results obtained were analyzed.RESULTS:In the supplement group, a significant decrease was observed both in the serum leptin and in CAT score after theintervention (p<0.05). Moreover, the FEV1 levels increased significantly in the supplement group after the intervention (p<0.05).Although, the difference between the two groups were not significant.CONCLUSIONS:The use of CLA supplement can improve COPD patients’quality of life through decreasing the serum level ofleptinin their blood
Sexual Activity, Interest and Satisfaction in Older Women in Iran
Get PDFIntroduction:The world's population is aging and the issue of sexual activity in elderly people is still a taboo in many cultures. The purpose of this study was to examine the sexual activity, interest and satisfaction in older women attended the Health Care Centers in the city of Ardabil-Northern Iran. MethodThis Cross-sectional study was conducted on 380 married women over 60 years old who had been selected by convenient sampling method. Data were gathered anonymously by three questionnaires (Goldberg general health, FSFI and demographic information Questionnaires) and analyzed using SPSS software. Results:This study indicated that the mean age of participants was 71±6.8 years, 60% of the studied women had sexual activity and about half of them had interest in sex. Sexually active women were significantly younger (p<0.05) and more educated (p<0.001) than sexually inactive women. The participants’ sexual activity, interest in sex, emotional intimacy and sexual satisfaction with their husbands were significantly affected by the presence of children at home regardless of whether they are single or married (p<0.05).. Conclusion:This study approved that despite misconceptions, older age and sexuality do not contradict each other and high education and having a private environment facilitate the intimate relationships and elevate their sexual satisfaction. The result of this study could help health care providers to provide appropriate and effective sexual healthcare services to older women
Prevalence, correlates and comorbidities of feeding and eating disorders in a nationally representative sample of Iranian children and adolescents
No full textObjective
This study investigated the prevalence of feeding and eating disorders, and identified their correlates and comorbidities among children and adolescents.
Method
We used the nationally representative sample of the Iranian Children and Adolescents' Psychiatric disorders (IRCAP) survey, with 30,532 participants randomly selected by a multistage cluster sampling method. We employed the kiddie schedule for affective disorders and schizophrenia‐present and lifetime version (K‐SADS‐PL) semi‐structured face‐to‐face interview to screen for any psychiatric disorders, including feeding and eating disorders, and associated factors. We used multivariate binary logistic regression to analyze the data.
Results
Valid data from 27,111 participants were analyzed. The total prevalence of feeding and eating disorders among children and adolescents was 0.89 (0.81–1.10). In all types of feeding and eating disorders, the adjusted odds ratio was higher among girls (except binge‐eating disorder) and older adolescents but was lower among rural residents. The most common psychiatric comorbidities observed in children and adolescents with feeding and eating disorders were obsessive–compulsive disorder (20.2%), agoraphobia (20.2%), depressive disorder (16.4%), social phobia (10.1%), oppositional defiant disorder (10.1%), generalized anxiety disorder (9.4%), attention deficit hyperactivity disorder (7.5%), and conduct disorder (5.7%), which were significantly more common compared to their peers without feeding and eating disorders.
Discussion
Older age, female gender and living in an urban area are predisposing factors in feeding and eating disorders (in binge‐eating disorder, the male gender is a positive correlate). We suggest that future works pay attention to the role of gender, comorbidities and predisposing factors
The prostate cancer and its relationship to sexual transmitted dieses
Get PDFprostate cancer (PCa) is the most common cancer type in men. Some studies reported that sexual transmitted disease may play a role in the etiology of prostate cancer.
This study was conducted by searching keywordProstate cancer, sexually transmitted disease, STD, Trichomonas vaginalis databases.
Researchers have found evidence of a link between the cancer and trichomoniasis – a common parasite that is passed on during unprotected sexual contact. They show that trichomoniasis may have a potential role in the development of prostate cancer.Inflammation plays a part in cancer development and growth. protein that stimulate inflammation in humans (called human macrophage migration inhibitory factor, HuMIF), is increased in prostate cancer.
Is not clear evidence whether there is a link between trichomoniasis and prostate cancer. This arguably suggests that the disease may arise due to a combination of complex risk factors. Further research will be required to investigate whether there is a clear link between trichomoniasis and prostate cancer. Despite the uncertainty of the current situation, Condoms are the most reliable method of protecting both partners from sexually transmitted infection
Investigation of the Effects of Hydroalcholic Extract of Figwort (Scrophularia Striata) on Testicular Parameters in male rats exposed Diazinone
Get PDFBackground and Objective: The vast majority of the native plants of Iran have medicinal properties such as antioxidants properties, anti-inflammatory, antimicrobial, and anti-tumor. It is been several years that Scrophularia Striata is considered as a traditional plant in different medical treatments. However, comprehensive studies on its effects on reproduction is still lacking in the literature. The present study is aimed at, investigating the possible protective effects of the Scrophularia Striata against hormonal and testicular changes in comparison to the proven effects of the vitamin E on Diazinon exposed male Rats.
Methods: After reverse light/dark cycle adaptation (12 hours) of 42 tree months aged mature Wistar male Rats, on average weight of 300g, they are divided into six random groups. The control group did not exposed on any drug. For the second group, Dimethyl sulfoxide solvent (0.1%) is injected. For the third group Diazinon (30 mg/kg/day), fourth group Scrophularia Striata (200 mg/kg/day), fifth group Diazinone (30 mg/kg/day) with Scrophularia Striata (200 mg/kg/day), and sixth group Diazinone (30 mg/kg/day) with vitamin E (200 mg/kg/day) are intraperitoneally injected. The period of injections for all the groups were 56 days according to the Sexual cycle of the male Rat.
Results: Injection of DZN into male rats over 56 days decreased the weight of testes and rats, as well as the reduction of sexual parameters including reduction in the number and percentage of sperm motility compared to the control group. Also testicular injury followingDZN administration resulted in histological changes of testis, which were resolved by simultaneous administration of DZN and SS.
Conclusion: The results of the present study shown that, the hydroalcoholic extract of the figwort and vitamin E can protect the testicular tissue and sexual hormones changes against the cellular apoptosis, inflammation and produced free radicals due to the Diazinone metabolis
The role of hormones in renal disease and ischemia reperfusion injury
No full textThe patients with renal diseases, especially end-stage renal disease (ESRD), are at high risk of developing cardiovascular disturbances. Some hormones such as brain natriuretic peptide appear to be important serum biomarkers in predicting cardiac death in ESRD patients. Renal diseases cause inflammation, anemia, uremic toxins, fluid overload, and electrolyte disturbance. Kidney transplantation is considered the choice treatment for patients with ESRD. Ischemia-reperfusion (IR), which occurs during renal transplantation is one of the factors that affect the outcome of renal transplantation. Renal graft rejection is the result of IR injury and there is no effective treatment to prevent IR injury. Reperfusion after ischemia may cause injury through generation of reactive oxygen and nitrogen species, inflammatory responses by increased levels of tumor necrosis factor-α (TNF-α) and interleukins (IL), and apoptotic processes, and leads to acute kidney injury (AKI). Thus, antioxidant, anti-apoptotic and anti-inflammatory hormones, which inhibit these pathways, can protect against IR injury and improve transplanted renal function in patients with ESRD
The study of screening autism spectrum disorder among 2 to 5 years old children in Ardabil between the year 2016-2017
Get PDFBackground and objective: Autism is a kind of developmental disorder that characterized by social impairment, communication difficulties, and restricted, repetitive, and sterotyped patterns of behavior. Considering the poor prognosis of this disease and the lack of awareness among Iranian society, the welfare organization of the country has begun screening of children aged 2 to 5 years in the country and Ardabil province. The purpose of this study was to determine the results of screening of autism spectrum disorder in children aged 2-5 years in Ardabil during 2016-17.
Methods: This cross-sectional descriptive-analytic study was conducted on all children with autism spectrum disorder in 2016-17 in Ardabil. The coordination was carried out with the Welfare Organization. Satisfaction and written consent were obtained from the Welfare Organization. Data collection forms were designed and duplicated and the required information was collected from patients. Incomplete cases were completed in partnership with parents. Written consent from parents was obtained by the Welfare Organization. In all stages of data collection, ethical measures such as confidentiality and privacy were observed. The Children's Autism Assessment Scale (CARS) was used to assess autism spectrum disorder in children
Results: A total of 23 children with autism spectrum disorder were considered, of whom, 16 (69.6%) were male and 7 (30.4%) were female. Mean age of the children was 3.13±1.1 years. The mean of total score of these children in CARS was 38.88 ± 5.8 and the highest scores of children belonged to the following subscales: fear and nervousness (2.89 ± 0.89), verbal communication (2.87 ± 2.8), activity level (2.87 ± 0.56), object use (2.84 ± 0.84), imitation (2.69 ± 0.77) and level and consistency of intellectual response (2.67 ± 0.55). The severity of autism was mild to moderate in 6 cases (26.1%) and severe in 17 cases (73.9%).
Conclusion: The findings of this study showed that the frequency of autism spectrum disorder is higher among males and is sever in the vast majority of patients. These findings show the importance of providing immediate and appropriate treatment interventions for these children