Ardabil University of Medical Sciences
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Degradation of diazinon from aqueous solutions by electro-Fenton process: effect of operating parameters, intermediate identification, degradation pathway, and optimization using response surface methodology (RSM)
No full textIn this experimental study, the statistical response surface methodology (RSM) based Box-Behnken design (BBD) was selected for evaluation of the effect of different parameters (solution pH,H2O2 concentration, current density, and time) and their interactions on the degradation of the diazinon by electro Fenton process. The results showed that the quadratic model is suitable for the obtained data (P-value = 0.0001) and in optimal process performance conditions was set equal to 100%. Regression analysis with a high correlation coefficient (R2 = 0.9980) and the model value of 765.56 indicated the adequacy of the model. Based on the results, the electro-Fenton process is an efficient technique for removing diazinon from aqueous media
Modeling and Intermolecular Binding Analysis of Novel 5-Aryl-1,3,4-Oxadiazole Derivatives with some Macromolecular Cancer Relevant Targets
Get PDFIntroduction: Cancer, the uncontrollable growth of abnormal cells, is the second leading cause of death following cardiovascular diseases. Further progression of basic sciences and introduction of novel drug design techniques and possibility of predicting ligand-receptor interactions, has led to more attempts to discover, design and develop new anticancer chemicals with the hope of accessing to new drugs to complete the cancer treatment process. 1,3,4-Oxadiazole derivatives due to their unique chemical structure and biological/pharmacological applications, are known as one of the centers of attention in medicinal chemistry.
Materials, Instruments and Methods: A few novel previously synthesized 2,5-disubstituted 1,3,4-oxadiazole derivatives (1-17) were subjected to combined systematic docking/quantum mechanical studies against certain previously proven chemotherapeutic receptors. AutoDock4.2 and ORCA quantum chemistry packages were used for modeling studies while LigPlot and Viewer Lite software were applied for obtaining ligand-target binding patterns.
Results and Discussions: In the current project, it was tried to explore binding modes/affinities of experimentally validated 1,3,4-oxadiazoles (1-17), to some validated chemotherapeutic targets. Structure binding relationship (SBR) studies showed that chemical structures possessing halogen atoms on 5-substituted phenyl and N-benzyl rings (4 and 17) exhibited superior binding modes/energies with regard to the majority of studied targets, regardless of their cytotoxic activity. A few oxadiazole structures exhibited ΔGbs comparable to or stronger than crystallographic ligands that were previously demonstrated to inhibit intended targets. On the basis of obtained results, a general SAR/SBR for binding of candidate oxadiazoles to binding sites of relevant targets was developed and a few top-ranked 2,5-disubstituted 1,3,4-oxadiazole structures were proposed as potential cytotoxic candidates that were also virtually validated. Moreover; lowest binding energy in the B3LYP/Def2-SVP level of calculation could be estimated for Arg486 (-6.05 kcal/mol) in binding of compound 17 to telomerase.
Conclusion and Suggestions: Various studies have demonstrated 1,3,4-oxadiazole heterocyclic nucleus as a privileged medicinal scaffold. A series of novel 2,5-disubstituted 1,3,4-oxadiazole derivatives were elucidated for their intermolecular binding patterns with some of the cancer relevant oxadiazole-inhibited targets. On the basis of obtained results, a general SAR/SBR pattern for candidate 1,3,4-oxadiazoles was offered and some hybrid oxadiazole structures were proposed as potential cytotoxic agents. Since the assessed macromolecular targets were previously proved to be blocked by 1,3,4-oxadiazoles, the results of this study might be useful in further design of more potent anticancer 1,3,4-oxadiazole derivatives through extending the scope of privileged structures toward designing new potential anti-tumor compounds
Frequency of the Obstructive Sleep Apnea symptoms in men with fertility disorders referring to the Caucasus infertility center in Ardabil during 2019 to 2020
Get PDFBackground: Infertility is defined as the failure to achieve a clinical pregnancy after 1 year of regular unprotected sexual intercourse.For many couples, it is a crisis and the cause of psychological stress. According to the studies has been done on animals, the reseachers found the obstructive sleep apnea has an effect on sperm quality.
Aim:The present study aimed at investigating the frequency of the obstructive sleep apnea symptoms in men with fertility disorders referring to the Caucasus infertility center in Ardabil during 2019 to 2020.
Matherials and Methods: We performed a cross sectional study, screening 100 men with infertility disorders to determine the frequency of obstructive sleep apnea symptoms based on the Berlin questionnaire. Convenience sampling methods were used and divided into two groups of high risk and low risk of obstructive sleep apnea. The data were collected using demographic and Berlin questionnaires and were analyzed with Spss software.
Results: The frequency of snoring and daytime sleepiness in infertile men with the high risk of the obstructive sleep apnea was 100% and 44.4%, respectively, and in men with infertility disorders was 46% and 33%, respectively. Among men who snored, those at high risk for obstructive sleep apnea showed a further decrease in total sperm count, progressive sperm motility and normal sperm morphology than those at low risk for obstructive sleep apnea.
Conclusion: Frequency of symptoms and risk of OSA are noticeable so that they can affect on sperm parameters
The study of surgical treatment results of the atrophic mandibular fracture with rigid fixation at inferior border
Get PDFBackground & Objective:
Today, due to the overall decline in edentulous and improved quality of life of many older people, increasing age and population growth, the number of edentulous people has remained stable, which has increased ageing -related injuries, such as mandible associated with edentulous And there has been an increase in edentulous mandibular atrophic fractures compared to the past. Due to life expectancy, population growth, systemic disease in the elderly, as well as the lack of studies with a sufficient number of patients with atrophic mandibular fractures, treatment of edentulous atrophic mandible with severe atrophy is a challenging topic. Therefore, in this study, we decided to evaluate the results of treatment of mandibular atrophic fracture surgery with hard fixation in patients referred to the Ardabil Oral and Maxillofacial Surgery Department who have been treated. Methods: In this case series, the medical records of all patients with atrophic mandibular fractures who referred to Fatemi Hospital in Ardabil between 2013 to 2019 and were treated with rigid fixation by 2 mm and 2. 4 mm plates at inferior border of mandible were reviewed. Due to the low number of patients, all patients were evaluated as a population by the complete enumeration method. After re-visit and preparation of panoramic radiography, the results of radiographic examination along with data extracted from the file and re-visit of patients were entered in the pre-checklists. Then the data were analyzed in SPSS 19. software.
Results:
From 15 patients included in this study ، there were 8 males (53. 3%) and 7 females (46. 7%) with age range 44-84 years (median:65. 8 years). 53. 3% of patients had underlying diseases. Most fractures reason were caused by falls (40%). Out of a total of 28 fractures ، the mandibular body was the most common (57. 4%) localization of the fractures that 73. 3% were bilateral, And was often (85. 7%) reported as a simple fracture. In this study, the most common complication was paraesthesia (33. 3%) and just a patient presented with postoperative nonunion and plate fracture, so rigid fixation was considered satisfactory in 93. 3% with 33. 3% of complications.
Conclusion:
Rigid fixation at inferior border (ORIF), is a successful and predictable protocol for treating atrophic edentulous mandible fracture. so it can be used typically in all patients with atrophic mandibular fractures. Immediate bone grafting should not be considered mandatory
Synthesis and cytotoxicity evaluation of novel cyclic/non-cyclic N-aryl enamino amides against human cancer cell lines
Get PDFBackground and purpose: Considering the undesirable consequences of prevalent cancer diseases, design and development of potent and selective anticancer chemotherapeutics is a major concern. Several studies have unraveled the potential of dihydropyrimidinone (DHPM) scaffold toward generating anticancer agents. Experimental approach: In the present work, a series of new dihydropyrimidinethiones (DHPMTs) along with a few acyclic enamino amides were synthesized and evaluated for their cytotoxic activity against human gastric (AGS), liver (Hep-G2), and breast (MCF-7) cancer cell lines. Findings/Results: Among the assessed compounds, one of the DHPMT derivatives (compound 5: 4-(3fluorophenyl)-6-methyl-N-phenyl-2-thioxo-1,2,3,4-ttrahydropyrimidine-5-carboxamide) exhibited superior cytotoxicity in all of the target cell lines (AGS, IC50 9.9 µM; MCF-7, IC50 15.2 µM; and Hep-G2, IC50 40.5 µM). Cytotoxicity assessments showed that non-cyclic enamino amides exhibited weaker activities when compared to cyclic analogues (DHPMs). Conclusion and implications: DHPMTs were better cytotoxic agents than non-cyclic enamino amides. Structure activity relationship studies guided us toward the design of DHPMT derivatives with OH and NH groups particularly on meta position of 4-phenyl ring and hydrophobic bulky substituents on carboxamide side chain within the structure. Possible interaction with the hydrophobic site(s) of the cellular target was supposed. The results of this study emphasized the potential role of DHPMTs and their optimized derivatives as privileged medicinal scaffolds to inhibit the growth of gastric, breast, and liver cancer cells
Assessment of Ardabil Dental Students Satisfaction from Evaluating with Log book in Paediateric Department in 2019-2020
Get PDFIntroduction: One of the evaluation methods that has become increasingly popular among educational professionals is the use of logbook, which is known as a suitable tool for the evaluation of educational goals. The aim of this study was to investigate the satisfaction of Ardabil dental students with the evaluation of logbooks in the pediatric department.
Materials and Methods: In this cross-sectional study, the satisfaction of all dental students in Ardabil in the first semester of the academic year 1398-99 in the pediatric department was evaluated by a Logbook using a 16-item questionnaire in 9 scopes (fairness, compliance with learning objectives, appropriateness, Time, feasibility, skills development, objectivity, stress, and interest in the evaluation method). Data were edited with SPSS software version 22. Significance level was also considered 0.05.
Results: The results of this study showed that students in all 9 scopes have a relatively high level of satisfaction. As the age of the participants increased, the satisfaction with the improvement of the evaluation skills, the evaluation method in reducing the students' stress and the evaluation method in the interest in employing the students in other sections also increased. There was a significant relationship between the adequacy of evaluation time and the average score of the participants and with increasing the average of score, the level of satisfaction increased. No significant relationship was found between other components.
Conclusion: According to the results of the present study, students of Ardabil Dental School had good satisfaction with the evaluation of the log book in the pediatric department
The effects of Vaccinium arctostaphylos extract attached to zinc oxide nanoparticles on expressions of interleukin-1 beta, Matrix metalloproteinases 2 and 9 in gastric cancer cell lines
No full textBackground: Gastric cancer is the third leading cause of cancer deaths worldwide. One of the leading causes of death in patients with gastric cancer is the very high ability of gastric cancer cells to invade other tissues and metastasize. MMP-2, MMP-9 and IL-1β are the major factors that play a key role in the invasion of cancer cells. Using metal nanoparticles and nano biomolecules production in medical research is a new method that increases the effectiveness and permeability of natural compounds. In this study, we used biosynthesis of ZnO nanoparticles (ZnO NPs) using Vaccinium arctostaphylos (VA) extract. It has been shown that blueberries to be effective against cancer due to polyphenolic, flavonoid and anthocyanin compounds. There is limited research on the anti-cancer effects of VA and its anti-cancer mechanisms are not yet fully understood. Our study focused on the effect of this plant extract on the factors affecting metastasis and invasion of cancer cells.
Aim: Evaluation the effect of VA-extract attached to ZnO NPs on the expression of MMP-2, MMP-9 and IL-1β involved in the invasion of gastric cancer cells
Materials and Methods: In this experiment, 14 groups including a control group, two groups treated with different doses of VA-extract, two groups treated with different doses of ZnO NPs and two groups treated with different doses of VA-extract attached to ZnO NPs were evaluated in AGS and MKN-45 cell lines. MTT assay was used to determine cell viability in both cell lines. Green synthesis method was used to biosynthesis of ZnO NPs using VA-extract. The results of nanoparticle biosynthesis were confirmed by EDX, XRD and SEM methods. Real-time PCR was used to evaluate the expression of MMP-2, MMP-9 and IL-1β genes. ELISA method was used to measure the values of MMP-2 and MMP-9.
Results: The results of MTT test showed that all three compounds of VA-extract, ZnO NPs and VA-extract attached to ZnO NPs caused cell death in both cell lines. The results of our study based on one-way analysis of variance (ANOVA) showed that in all three groups including VA-extract, ZnO NPs and VA-extract attached to ZnO NPs, the expression of MMP-2, MMP-9 and IL-1β genes in AGS cell line reduced compared to the control group (p<0.001). In AGS cell line, 30 µg/ml dose of VA-extract attached to ZnO NPs was more effective compared to other groups (p<0.001). In MKN-45 cell line, the expression of MMP-2, MMP-9 and IL-1β genes reduced in the VA-extract and VA-extract attached to ZnO NPs groups compared to the control group (p<0.001), while the expression of MMP-2, MMP-9 and IL-1β genes increased in different doses of ZnO NPs compared to the control group (p<0.001).
Conclusion: Our results showed that VA-extract and VA-extract attached to ZnO NPs reduced cell viability and expression of MMP-2, MMP-9 and IL-1β genes in both AGS and MKN-45 cell lines. However, VA-extract attached to ZnO NPs significantly reduced expression of all three gene
Rosuvastatin suppresses cytokine production and lung inflammation in asthmatic, hyperlipidemic and asthmatic-hyperlipidemic rat models
No full textBackground: Given the role that T lymphocytes play on the pathogenesis of allergic asthma, drugs targeting Th2 and Th17 cells may be a hopeful therapeutic strategy. This study aimed to evaluate the effect of rosuvastatin treatment on cytokine production and lung inflammation in allergic asthma.
Methods: The animals were assigned into control (C), asthmatic (A), hyperlipidemic (H), asthmatic-hyperlipidemic (AH), rosuvastatin (40 mg/kg/day intraperitoneally, for 3 weeks)-treated asthmatic (AR), rosuvastatintreated hyperlipidemic (HR) and rosuvastatin-treated asthmatic- yperlipidemic (AHR) groups (n = 6 in each group). The levels of IL-4, IFN-γ and IL-17, total and differential WBC counts in bronchoalveolar lavage fluid (BALF), Th1/Th2 balance, and pathological changes were evaluated.
Results: The BALF level of IL-4 in A, H and AH groups, and IL-17A in A and AH groups were significantly higher than that in C group (p < 0.05 to p < 0.001). IFN-γ level and Th1/Th2 balance (IFN‑γ/IL-4 ratio) in A and AH groups were significantly decreased (p < 0.05 to p < 0.01). Inflammatory cells infiltration, muscle hypertrophy and emphysema were also observed in A and AH groups. The BALF levels of IL-4 in AR, HR and AHR groups, IFN-γ level in HR group, and IL-17A level in AR and AHR groups showed a significant improvement
compared to that of A, H and AH groups (p < 0.05 to p < 0.001). Rosuvastatin treatment increased Th1/Th2 balance in all treated groups (p < 0.05 to p < 0.01), decreased total WBC counts, neutrophilia, eosinophilia and lung inflammation in AR and AHR groups, and improved muscle hypertrophy and emphysema in AHR group.
Conclusions: Rosuvastatin treatment improved lung pathological changes by suppression of Th2 and Th17- mediated cytokines which was unrelated to its lipid-lowering activity. Therefore, rosuvastatin might be a candidate immunomodulatory drug for treatment of patients with allergic asthm
The Relationship between Quality of Life and Physical Activity, Worry, Depression, and Insomnia in Pregnant Women
Get PDFObjective: Physical activity (PA), insomnia, depression, and worry were the key factors affecting pregnant women’s
quality of life (QoL). The present study aimed to determine quality of life and its relationship with physical activity,
insomnia, depression, and worry in pregnant women.
Method: This was an observational cross sectional study, conducted among 256 healthy pregnant women using 5
questionnaires: WHOQOL-brief (WHO Quality of Life Questionnaire, brief version, ISI (Insomnia Severity Index), PSWQ
(Penn State Worry Questionnaire), ZSRDS (Zung Self-Rating depression Scale), and Pregnancy Physical Activity
Questionnaire (PPAQ).
Results: There was a significant relationship between general QoL, insomnia, and worry with educational background,
number of children, and occupation (P < 0.05). Depression had a significant relationship with occupation (P < 0.05). PA
did not have a significant relationship with demographic information. However, insomnia had a significantly negative
relationship with general QoL, general health, and psychological health (P < 0.05). The worry variable had also a
significant negative relationship with general QoL, general health, and physiological health (P < 0.05). Depression had a
significant negative relationship with general health, physical health, and psychological health (P < 0.05). There was no
significant relationship between physical activities in pregnancy with QoL, depression, worry, and insomnia.
Conclusion: Women need to be informed about the necessity of controlling and reducing insomnia, worry, and
depression to have a higher QoL. PA declined during the second and third trimester of pregnancy. However, PA in
pregnancy can positively impact general QoL
The Relationship Between Obesity, Overweight, and the Human Development Index in World Health Organization Eastern Mediterranean Region Countries
Get PDFObjectives: Obesity is regarded as one of the most prominent health threats worldwide and a serious risk factor for non-communicable diseases, such as diabetes mellitus type 2, high blood pressure, cardiovascular diseases, and some types of cancer. Given the role that societal development—as reflected by the Human Development Index (HDI)—may play in the prevalence of obesity and overweight, this study aimed to investigate the degree to which the prevalence of obesity and overweight is affected by HDI and its components.
Methods: In this ecological study, the required data on HDI and its components were gathered from the latest report of the United Nations Development Program, and data on obesity and overweight were acquired from the latest reports published on the World Health Organization website. Statistical analyses were conducted using SPSS version 24.0.
Results: The prevalence of obesity was determined to be significantly higher among females than males, and the gross national index per capita was found to be significantly higher for males than females (p<0.05). Significant positive correlations were found between HDI and its components and sex, as well as indices of obesity and overweight.
Conclusions: A significant positive correlation exists between HDI and obesity. As policy-makers attempt to improve the general welfare of the people, they should be aware of potential unwanted effects of development on the risk of obesity and overweight among the population