Ardabil University of Medical Sciences
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Evaluation of diagnostic value of serum level of Hepcidin, total Bilirubin and Neutrophil to Lymphocyte ratio in diagnosis of Pediatric complicated acute appendicitis
Get PDFBackground: In children, abdominal pain is one of the most important and significant issues. One of the most common causes of abdominal pain in children is acute appendicitis. The course of acute appendicitis is faster in children and has a higher chance of perforation and peritonitis than in adults.
Aim: Determining the diagnostic value of serum hepcidin and total bilirubin and neutrophil to lymphocyte ratio in the diagnosis of acute complicated appendicitis in children
Methods and materials: The present study was a cross-sectional study. The statistical population of the present study includes patients aged 6 to 16 years who underwent emergency appendectomy with a diagnosis of appendicitis. The sampling method was census in a period of 2 months from all patients between 6 and 16 years who underwent appendectomy. Laboratory parameters including hepcidin, total bilirubin and blood leukocyte levels were recorded in researcher-made checklists at the time of admission of these patients and after collection were analyzed by SPSSv22 statistical analysis software.
Results: Eighty seven 6-16 YO patients who underwent emergent appendectomy were enrolled in this study. Among the patients, 70 patients had inflamed appendix and 17 had not. From 70 appendicitis patients, 47 (67.1%) were male. According to our findings, average levels of hepcidin, total bilirubin and neutrophil to lymphocyte ratio was statistically higher in patients with inflamed appendix than the other group. Sensitivity and specificity of hepcidin was 53.3 & 90.9, bilirubin was 46.7 & 89.1 and neutrophil to lymphocyte ratio was 100 & 83.6 in differentiating complicated appendicitis from simple appendicitis.
Conclusion: Finally, it can be concluded from the findings of the present study that the combination of laboratory indicators of total bilirubin, hepcidin and neutrophil to lymphocyte ratio can be used as an indicator in the diagnosis of complicated appendicitis in childre
Study the frequency of bronchial anthracosis and its influencing factors in patients undergoing bronchoscopy in Ardabil city hospital, 2013-2015
Get PDFBackground: Bronchial anthracosis is defined as appearance of multiple dark anthracotic pigmentations on large airway mucosa with or without airway narrowing or obliteration. This study aims to investigate the frequency of bronchial anthracosis and its influencing factors in patients undergoing bronchoscopy.
Methods: In this cross-sectional study, data was obtained from reviewing the files of 900 patients who underwent bronchoscopy in Ardabil city Hospital. Those with dark pigmentation on their airways mucosa were considered as having bronchial anthracosis. During bronchoscopy, samples were collected from the airways in the form of bronchoalveolar lavage and then the smear and culture of these samples were examined for acid fast bacilli. Type of fuel used for cooking in the kitchen and for heating in their house, history of smoking, the patient’s geographical location and occupation were recorded by a checklist and then evaluated.
Results: Of 900 cases, 42 (4.6%) had bronchial anthracosis, out of which 23 (55%) were male and most were in urban areas. Majority of patients were in the age range of 60 to 70 years. Of 11 farmers with anthracosis, 7(6.63%) patients used fossil fuels for heating. Among the anthracosis patients, 11 (21.2%) subjects had Tuberculosis. There was a significant difference between age, habitant of patients, pulmonary tuberculosis and bronchial anthracosis.
Conclusions: There was a significant correlation between age of patients, pulmonary tuberculosis and bronchial anthracosis, therefore, performing necessary tests and follow-ups for pulmonary tuberculosis is necessary in cases that undergo bronchoscopy for any reason and those with bronchial anthracosis
Efficacy of Metformin and Chemotherapeutic Agents on the Inhibition of Colony Formation and Shh/Gli1 Pathway: Metformin/Docetaxel Versus Metformin/5-Fluorouracil
No full textBackground Gastric cancer is a common gastrointestinal cancer characterized by poor prognosis and chemoresistance. Docetaxel and 5-fluorouracil (5-FU) are frequently used for the treatment of gastric cancer. Despite their potent anti-cancer effects, chemoresistance occurs in metastatic gastric cancer. Metformin, a popular anti-diabetic drug, has been proven to have potent anticancer effects on gastrointestinal cancers. Here, we aim to improve this chemotherapy agents’ efficacy by pretreatment with metformin.
Methods The AGS gastric cancer cell line were pretreated with three different sub-toxic concentration of metformin and then treated with various concentrations of 5-FU and docetaxel.
The anticancer effects of the combination of metformin with the chemotherapy agents were determined using clonogenic assay and DAPi staining. We used real-time PCR to evaluate Gli1, Gli2, and TWIST1 mRNA expression levels in the gastric cancer cells. Also, the expression of the Shh protein was assessed using immunocytochemistry.
Results Here, we found that metformin sensitized the gastric cancer cells to chemotherapy. The combination treatments were more effective in reducing the number of cancer colonies compared to 5-FU or docetaxel alone. The combination of metformin with 5-FU or docetaxel significantly reduced the number of cells expressing the Shh protein compared to the 5-FU alone or docetaxel alone. Interestingly, we found that the combination of metformin with docetaxel significantly down-regulated the mRNA levels of Gli1, Gli2, and TWIST1 in the AGS gastric cancer cell line compared to docetaxel alone.
Conclusion Overall, our data strongly support an important role for metformin as an enhancer of the efficacy of chemotherapeutic agents against gastric cancer
Prevalence of anti-Toxoplasma gondii antibodies in HTLV-infected individuals
No full textObjective: Human T-cell lymphotropic virus (HTLV) infection is highly prevalent in Mashhad (2.5%) and can cause various diseases related to the immune system. Since Toxoplasma gondii can remain latent in the body and can be reactivated in people with immune system defects, this study was performed to determine the frequency of IgG anti-Toxoplasma gondii antibody in HTLV-infected individuals and control group.
Methods & Materials:
This study was performed on serum samples of 236 people infected with HTLV virus. Serum samples of HTLV-infected individuals were evaluated for the presence of anti-Toxoplasma gondii IgG antibodies using ELISA method. Serum samples from 291 non-HTLV-infected individuals were also tested for the presence of anti-Toxoplasma gondii IgG antibodies.
Results: The mean age of the subjects was 50.03 years. In the case group 66.8% were female and 33.2% were male and in the control group 55.2% were female and 44.8% were male. Anti-Toxoplasma gondii IgG was 62.3% positive in HTLV group and 50.2% positive in control group. The frequency of anti-Toxoplasma gondii antibody in the two groups showed statistically significant difference. In both groups, the prevalence of anti-Toxoplasma gondii antibody was higher in women than men, but the difference between the two groups was not statistically significant.
Conclusion: The frequency of anti-Toxoplasma gondii IgG was higher in the HTLV positive group compared to the control group, so it is important to pay special attention to the reactivation of this parasite in people infected with HTLV, especially if they are women of childbearing age
The Relationship between Nesfatin-1 Serum Levels with Epicardial Fat Thickness in Patients with Acute Myocardial Infarction (AMI) and Comparison with Patients with Stable Angina
No full textBackground: Nesfatin-1 is a peptide with metabolic effects that has recently been revealed to be closely related to with a variety of chronic inflammatory diseases.
Aim: The aim of the present study was to investigate the association between nesfatin-1 levels and angiographic, biochemical, and echocardiographic findings, especially with the epicardial fat thickness (EFT) in patients with coronary artery disease.
Methods and Materials: In a cross sectional study, three groups were selected from 90 patients candidate for angiography, including 30 subjects as a control group with normal angiography but a history of chest pain, 30 patients with a diagnosis of stable angina pectoris (stable-AP), and 30 patients with a diagnosis of acute myocardial infarction (acute-MI). Parameters measured in all subjects included demographic, biochemical, echocardiographic, and angiographic.
Results: The results showed that serum levels of nesfatin-1 in acute-MI and stable-AP groups were significantly reduced compared to the control group (P < 0.001 and P < 0.01, respectively), but there was no significant difference between acute-MI and stable-AP groups. In addition, the findings demonstrated that there was a negative association between nesfatin-1 and LDL-C, Gensini score, white blood cell, troponin T, EFT, and CK-MB, but there was a positive association with left ventricle ejection fraction (%).
Conclusion: Decreased levels of nesfatin-1 in acute-MI and stable-AP groups as well as its association with several parameters such as EFT, CK-MB, and Gensini score, may indicate the potential role of nesfatin-1 in the process of atherosclerosis, which requires further study
Identifying the content and form challenges of Ashura and Arbaeen literature in the field of praise
No full textDevelopment of CYFRA 21-1 sensor based on molecularly imprinted polymer
No full textIntroduction:
Early detection of lung cancer can play a vital role in the successful treatment of patients. Cancer biomarkers are valuable tools in the early diagnosis, evaluation of chemotherapy and demonstration if recurrence following chemotherapy. CYFRA 21-1 is an important biomarker for diagnosis of lung cancer. To reach rapid and affordable detection of CYFRA 21-1, biosensors are robust tools for easy detection of cancer biomarker. To develop biosensors, molecularly imprinted polymers (MIPs) as emerging recognition elements can be used for selective detection of analyte molecules. In this work, we demonstrated the development of selective MIP-based biosensor for ultrasensitive detection CYFRA 21-1 antigen.
Materials and Methods:
First, a screen-printed electrode (Au-SPE) as a working electrode was cleaned using acetone and de-ionized water three times and was dried by N2 stream. Then, the Au-SPE was modified with a thin layer of graphene. Carboxyl groups on graphene sheets were activated by EDC-NHS, and monomers of acrylamide were introduced to the activated graphene sheets, and in presence of CYFRA 21-1, acrylamide, bis-acrylamide and TEMED polymerization of MIP was carried out by applying potentials ranging from -1.3 to -0.1 V. Finally, the analyte molecules were removed from the polymer matrix by incubating in the trypsin solution. To characterize the prepared biosensor, atomic force microscopy, cyclic voltammetry and electrochemical impedance spectroscopy were used.
Results and discussion:
The results of EIS analyses showed the acceptable stability, reproducibility and selectivity for CYFRA 21-1 biosensor. Limit of detection (LOD) and linear detection range (LDR) for this biosensor were found to be 6.7 pg/mL and in the range of 0.00001 - 100 µg/mL, respectively. This study provides a promising strategy to fabricate sensor devices based on MIPs with highly selective recognition ability, simplicity of operation, small size and low cost
Subgrouping University Students Based on Substance Use Pattern: A Latent Class Analysis
Get PDFPurpose: High-risk behaviors are the main causes of death and disability among youth and adults. Entering university might cause students to go through their first-hand experience of using substances.
Aim: This study aimed to detect the subgroups of students based on substance use and assess the effects of religiosity and parental support as well as other related factors on the membership of students in each latent class.
Methods: Using a multistage sampling method, this cross-sectional study was conducted in 2016 in Hormozgan University of Medical Sciences (n=524). All students completed a self-report questionnaire. This questionnaire contained questions about substance use, religious beliefs and familial support. The questions of substance use were prepared using the World Health Organization Alcohol, Smoking and Substance Involvement Screening Test (WHO ASSIST). To analyze the data, PROC LCA statistical method was run in SAS9.2.
Results: Three latent classes were identified: 1) nonuser (87.5%), 2) tobacco and illicit drug user (8.7%) and 3) polydrug user (3.8%). Having extramarital sex in the last month (OR=28.29, 95% CI; 8.45– 94.76), living alone (OR=4.29, 95% CI; 1.01– 18.35) and having a higher score of familial support (OR=0.94, 95% CI; 0.89– 0.98) were associated with the polydrug user class. Hookah smoking had the highest (11.1%) and non-medical methylphenidate use had the lowest (2.3%) prevalence among the participants of the study.
Conclusion: This study revealed that 12.5% of the students were either tobacco and illicit drug users or polydrug users. Thus, focusing on the religiosity and familial support may help design some preventive programs for this stratum of young adults
Study demographic and clinical characteristics of children with acute lymphoblastic leukemia in Ardabil province 2010-2018
Get PDFBackground: Leukemia is one of the most common malignancies in children which has an incident of 40 in every million. Leukemia accounts for 30% of all malignancies in children under 15 year old.
Aim: to determine demographic and clinical characteristics of children acute lymphoblastic leukemia in Ardabil province
Methods and meterials: The current study is a cross-sectional and descriptive one. 107 children who were diagnosed with ALL during 1389-1397 in Bou_Ali pediatric hospital of Ardabil city, were enrolled in the study. Their information including demographic, clinical manifestations and immunomorphology were gathered using a checklist after obtaining required permissions and been analyzed using SPSSv22.
Results: The results showed that most of the children suffering from ALL was in the age group of 1-10 YO. 61 children (57%) were male. 58 patients were from average economical level. In about 31% of the children, the season of disease onset was summer. In 75.8% of cases, the final diagnosis was made before one month. The most common manifestations were fatigue, organomegaly, fever, pallor and lymphadenopathy respectively. From the total 107, 69 cases were
Pre-B ALL type. Further evaluations revealed that WBC more than 50000 was occurred in only 18.7% of cases. Also, Hb less than 7 was shown in 31.8% of the patients. Plt count less than 100000 was seen in 79.4% of cases.
Conclusion: Finally, it can be concluded from the current study that ALL related manifestations in Ardabil province is similar to what is described globally. One of the most important conclusions from this study is the early diagnosis of the disease before development of blastic crisis which can positively affect the survival of the child so that more that 80% of cases, the WBC was less than 100000
The habitat suitability model for the potential distribution of Ornithodoros tholozani (Laboulbène et Mégnin, 1882) and Ornithodoros lahorensis (Neumann, 1908) (Acari: Argasidae): the main vectors of tick-borne relapsing fever in Iran
No full textEndemic relapsing fever (RF) is one of the most important arthropod-borne diseases caused by various
types of Borrelia and transmitted by soft tick species. The investigation of the distribution of vectors in a region can
help control and prevent the disease. This study aimed to investigate the distribution of Ornithodoros tholozani and
Ornithodoros lahorensis in Iran and to identify the most influential climatic variables affecting their distribution. The
ecological niche model was used in Maxent to predict the environmental suitability of the studied species. A review was
conducted on the earlier studies carried out in Iran (1977–2018), and the coordinates of collection sites for these two
ticks were recorded. Nineteen bioclimatic variables were used for the modelling. The main vectors of RF were reported
from 13 provinces, 43 counties and more than 160 villages in Iran. The rate of Borrelia spp. infection was higher in O.
tholozani (36%) than in other soft ticks. The annual mean temperature and precipitation seasonality were the most
important factors affecting the distribution of RF vectors. The north-western regions of Iran were found to provide the
best environmental needs for these vectors. Therefore, special attention should be paid to control the disease by
managing contact with soft ticks in these areas