Ardabil University of Medical Sciences
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Role of cerebrospinal fluid in differentiation of human dental pulp stem cells into neuron-like cells
Get PDFHuman dental pulp stem cells (hDPSCs) could be differentiated into neuron like-cells under particular microenvironments. It has been reported that a wide range of factors, presented in cerebrospinal fluid (CSF), playing part in neuronal differentiation during embryonic stages, we herein introduce a novel culture media complex to differentiate hDPSCs into neuron-like cells. The hDPSCs were initially isolated and characterized. The CSF was prepared from the Cisterna magna of 19-day-old Wistar rat embryos, embryonic cerebrospinal fluid (E-CSF). The hDPSCs were treated by 5% E-CSF for 2 days, then neurospheres were cultured in DMEM/F12 supplemented with 10-6 μm retinoic acid (RA), glial-derived neurotrophic factor and brain-derived neurotrophic factor for 6 days. The cells which were cultured in basic culture medium were considered as control group. Morphology of differentiated cells as well as process elongation were examined by an inverted microscope. In addition, the neural differentiation markers (Nestin and MAP2) were studied employing immunocytochemistry. Neuronal-like processes appeared 8 days after treatment. Neural progenitor marker (Nestin) and a mature neural marker (MAP2) were expressed in treated group. Moreover Nissl bodies were found in the cytoplasm of treated group. Taking these together, we have designed a simple protocol for generating neuron-like cells using CSF from the hDPSCs, applicable for cell therapy in several neurodegenerative disorders including Alzheimer’s disease
Study of determining serum anti mullerian hormone and its affecting factors in infertile women undergoing IVF referring to infertility center of Ardabil University of medical sciences
Get PDFBackground: Infertility means inability of pregnancy after one year of natural continuous intercourse, not using any prevention which is one of the most important problems in every human societies. The average rate of infertility in different societies is been estimated to be 8-12%.
Aim:The aim of this study is to determine the level of AMH in IVF using infertile patients referring to the infertility center of Ardabil University of medical sciences and effective factors. We hope that the results of this study makes a positive step in diagnosis, treatment and prevention in these patients.
Methods and meterials: The current study was a cross-sectional one which were done using library and field methods. A number of 170 women with the diagnosis of infertility referring to the infertility clinic of Ardabil Alavi hospital during 1398 were enrolled randomly in the study and their clinical data including demographic, anthropometric and hormone levels were gathered and analyzed using SPSSv22 software.
Results: Mean age of studied women were 31.7±4.68 years. the age was compatible with normal distribution curve. Mean AMH level in the patients was 3.35±2 ng/ml. Analysis of the data using pearson correlation showed a strong association between two indices of age and AMH level (r=0.68, p<0.001). Analysis by pearson correlation showed that although it seems that with BMI increase, the level of AMH declines but this association was not statistically significant (r=-0.102, p=0.185). in women with the complaint of secondary infertility, prevalence of AMH deficiency was significantly lower than other women (p=0.045). There was no difference in AMH levels between regular and irregular monthly menses (p=0.275).
Conclusion: Finally it can be concluded from our study that increase of age is one of the main factors in AMH levels. Also, the deficiency of this hormone can be a cause in secondary infertility
Post-synthetic functionalization of the metal-organic framework: Clean synthesis, pollutant removal, and antibacterial activity
No full textHerein, a metal-organic framework (ZIF-8) was synthesized and functionalized with 3-aminopropyl trimethoxy silane (APTMS). The synthesized materials (ZIF-8 and ZIF-8@APTMS) were characterized using various techniques, including SEM, TEM, FTIR, Raman, XRD, BET, and TGA. Pollutant (Direct Red 23(DR23)) removal ability of the materials from aqueous media was investigated. In addition, the antibacterial activity of the synthesized materials was investigated with Staphylococcus aureus, and Escherichia coli by three methods, including diffusion agar, minimum inhibitory concentration (MIC)/ minimum bactericidal concentration (MBC), and viable cell count. The effect of possible interactions with key parameters on pollutant removal was studied. The results showed that the DR23 adsorption obeyed the pseudo-second-order kinetic and the Freundlich isotherm with the experimental adsorption data. The maximum adsorption capacity and removal efficiency of DR23 with ZIF-8@APTMS adsorbent was 2159 mg/g and 93.59 %, respectively. The data from this study concluded that ZIF-8@APTMS was significantly capable of dye adsorbing from aqueous media. The antibacterial activity of the materials is almost similar and is slightly higher in the ZIF-8 sample. The MBC was obtained for both bacteria with the same MIC numbers, indicating the toxicity of similar materials as an inhibition zone. The growth inhibition halo was more visible on S. aureus than in E. coli. The percentage of bacterial decrease in the viable cell count method was 92 % on S. aureus and 20 % on E. coli
culture of patient safety in nurses who working in corona wards of Imam Khomeini Hospital in Ardabil
No full textPrevalence of Abuse in Elders and Its Sociodemographic Association
No full textBackground
Elder abuse is a universal phenomenon. Elder abuse is one of the consequences of the increase in the number of seniors in today's families and the communities, and a great impact on the health and safety of the elderly
Objectives
Considering the importance of this issue and not uniform in various the factors affecting the abuse the current study aimed to evaluate the prevalence and associated factors of abuse among the elderly in Ardabil, Iran in 2020.
Methods
This cross sectional study was conducted on people over 60 years old in Ardabil, Iran. Data were collected using elder abuse questionnaire. Data were analyzed by SPSS version 22, Descriptive statistics, chi-square test and logistic regression. The significance level was P<0.05
Results
Out of 500 subjects participating in the current study, 258 (51.6%) were men and 242 (48.2%) were women. The mean age of the population under study was 69.15±7.27 years. Among 500 participants, 377(75.4%) reported that they had experienced at least one type of abuse in the past year. The highest rate of elder abuse was observed regarding Emotional abuse (47.2%) and psychological abuse (40.8%), and the lowest rate was measured for ostracizing (15.4%) and physical abuse (12.4%). Result showed that elder abuse was significantly associated with Having a chronic illness and Number of children 1-4.
Conclusion
Considering at least eight out of ten elderly people experienced on type of abuse. And factors such as number of children, home situation, illness background are involved recommended by an interdisciplinary approach and adopt appropriate strategies for a team with the discovery and identification of the victims, the creation of a coherent social and economic services, strengthening social support networks, formulate laws protecting seniors, great strides in order to solve this important health and social problem is remove
Evaluation of the Relationship between oral Cinacalcet in Inflammation (CRP) and Anemia in Hemodialysis Patients with Secondary Hyperparathyroidism
No full textBackground: Cinacalcet is one of the newest calcium mimetic drugs in the treatment of patients with parathyroid cancer, secondary hyperparathyroidism in adults with chronic kidney disease. Due to the possible association between the effect of Cinacalcet on CRP (C Reactive Protein) and the pathophysiology of anemia in ESRD (End Stage Renal Diseae)patients, we evaluate the results of oral Cinacalcet on CRP and anemia in hemodialysis patients with secondary hyperparathyroidism.
Aim: To determine the results of oral Cinacalcet therapy on CRP and anemia in hemodialysis patients with secondary hyperparathyroidism
Methods and material: In this study, all hemodialysis patients over 18 years old who underwent hemodialysis for at least 6 months that despite maximal phosphorus-reducing and rocaltrol treatment still had serum phosphorus greater than 5.5 mg / dl , serum calcium greater than 8 mg / dl , serum PTH were above 600 pg / dl ,and with secondary hyperparathyroidism diagnosis treated with Cinacalcet,were entered in study. The dose of receiving Eprex wasn’t changed at the beginning and end of the study. The number of patients was 40. All records of these patients were reviewed , the levels of hemoglobin ,Ca , P (every month), PTH, ferritin, TIBC, iron, CRP (every three months) before the start of treatment , according to the routine dialysis ward were repeated and were recorded in the files.In the demographic information checklist , Age, sex, underlying disease, duration of dialysis , duration of use and dose of cinacalcet were entered. The results of these experiments were reviewed and analyzed before and after treatment with cinacalcet.
Results: The findings of the current study reveal that the treatment with oral cinacalcet induced a significant increase in serum hemoglobin(P<0.05).Although the treatment reduced the level of serum CRP,this reduction wasn’t statistically significant.
Conclusion: Treatment with Cinacalcet in hemodialysis patients with secondary hyperparathyroidism, at least in this study, increased serum hemoglobin and decreased the level of serum CRP. It could be a hypothesis for large analytical studies
The Study of Oral Health Status and Its Related Quality of Life in Elderly People Supported by Welfare Organization in Ardabil City
No full textBackground & aim: Considering the increasing trend of elderly population in recent years
and the fact that oral health is an integral part of general health, this study aimed to assess the
oral health status and its related quality of life among elderly people living in hospices under
support of Ardabil’s welfare organization.
Methods: In this descriptive-correlational study, 144 elderly people over 60 years old, living
in hospices, were included in the study. A questionnaire was used for data collection with
three parts: Demographic form, World Health Organization (WHO) Oral Health Assessment
Form, and Oral Health Impact Profile (OHIP-14Per). Data were analyzed using descriptive
statistics, one sample T-test, ANOVA and linear regression in SPSS version 24.
Results: The results of one-sample T-test showed that the elderly in Ardabil have an
inappropriate status in all aspects of oral health-related quality of life (p<0.05). In addition,
regression analysis found a significant relationship between oral health status and oral healthrelated
quality of life in elderly people (p<0.05).
Conclusion: The results of this study showed that the elderly people living in hospices under
the supervision of Ardabil’s welfare organization are in a poor status regarding oral health and
oral health-related quality of life. Therefore, it is necessary for healthcare providers to pay
more attention to this group regarding their oral health and quality of life.
Keywords: Oral Health Status, Oral Health-related Quality of Life, Elde
Evaluation of Sleep Quality in Patients with Multiple Sclerosis: A Case-Control Study
No full textBackground and Objective: Studies indicate that people with multiple sclerosis (MS) are at increased risk of sleep disorders and the resulting outcomes. The aim of this study is to evaluate sleep quality in patients with MS.
Materials and Methods: In this case-control study, 110 patients with all of the confirmed types of MS as a case group and 110 healthy subjects as control group were evaluated in terms of sleep quality using Pittsburgh Sleep Quality Index (PSQI). The data collected was analyzed using t-test and chi-square tests.
Results: The total score of PSQI was significantly higher in patients with MS than that of the control group (7.32 ± 2.78 vs. 5.30 ± 2.00, P = 0.001). Furthermore, PSQI scores of patients with MS was significantly higher than that of the con-trol group in subscales including sleep latency (1.14 ± 0.99 vs 0.85 ± 0.91, P = 0.029), sleep duration (1.09 ± 0.80 vs. 0.83 ± 0.88, P = 0.048), habitual sleep efficiency (1.15 ± 0.92 vs. 0.85 ± 0.83, P = 0.017), sleep disturbance (??? ± 0.96 vs 0.51 ± 0.88, P = 0.013), use of sleep medication (0.69 ± 0.94 vs. 0.33 ± 0.65, P = 0.001), and daytime dysfunction (0.88 ± 0.93 vs. 32.2 ± 0.69, P = 0.001).
Conclusion: Patients with MS had a significantly more improper sleep quality compared to the control group. So, poor sleep quality has a negative effect on daily activities and social communication of these patients and generally reduce their quality of life (QOL)
Microbiological Profile and Antibiotic Resistance Pattern of Uropathogens in Diabetic and Non-Diabetic Patients
No full textBackground & Aims: Urinary tract infection (UTI) is one of the most common human infections which is more prevalent especially in patients with diabetes mellitus. The aim of this study was to compare the uropathogens isolated from the urine culture of diabetic and non-diabetic patients and their antibiotic resistance pattern in patients admitted to Imam Khomeini Hospital in Ardabil from 2012 to 2013.
Materials & Methods: In this descriptive cross-sectional study, the medical records of all patients admitted to Imam Khomeini
University Hospital in Ardabil province from the beginning of 2012 to the end of 2014 were reviewed and the required information including age, sex, or the absence of diabetes was recorded.
Results: E. coli was the most common uropathogens isolated from both diabetic (58.1%) and non-diabetic (53.6%) patients followed by yeast (19.4%) in both groups. Other common organisms in diabetic and non-diabetic patients were Staphylococcus aureus (8.4%) and coagulase negative Staphylococcus (7.1%), respectively. Among diabetic patients, E. coli had the highest sensitivity to polymyxin
(100%), tetracycline (100%), and amikacin (88.9%). In non-diabetic patients, E. coli had the highest sensitivity to amikacin (90.4%), nitrofurantoin (86%), cefoxitin (85.3%), and gentamicin (82.1%).
Conclusion: Our findings indicated that susceptibility profiles of uropathogens are different in diabetic and non-diabetic individuals, therefore, empirical treatment for diabetic and non-diabetic patients will be different