Ardabil University of Medical Sciences
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Effect of Conjugated Blackcurrant Extract and Zinc Oxide Nanoparticles on Histopathologic Damage of Kidney Tissue in Diabetic Rats
Get PDFBackground & objectives: One of the most important diabetes complications is chronic renal failure. This disorder is one of the most important progressive renal problems that is closely associated with high mortality rate in diabetic patients. The purpose of this study was to evaluate the effect of conjugated blackcurrant extract and zinc oxide nanoparticles on histopathologic damages of kidney tissue in diabetic rats.
Methods: A total of thirty adult Wistar rats were used and they were randomly divided into the following six groups (n=5): control group; diabetic group (170 mg/kg alloxan); diabetic group treated with insulin (170 mg/kg alloxan & 10 U/kg insulin); diabetic group treated with ZnO nanoparticle (170 mg/kg alloxan & 8 mg/kg ZnO nanoparticle); diabetic group treated with extract (170 mg/kg alloxan & 150 mg/kg blackcurrant extract) and diabetic group treated with extract conjugated with ZnO nanoparticles (170 mg/kg alloxan & 8 mg/kg blackcurrant extract conjugated with ZnO nanoparticle). Diabetes mellitus was induced by intraperitoneal injection of alloxan monohydrate. Conjugated blackcurrant extract and ZnO nanoparticles were produced using green synthesis method. The tissues were stained with hematoxylin and eosin stain. Image J software was used for evaluation of histopathological changes.Results: The comparison between groups treated with blackcurrant extract, ZnO nanoparticles and blackcurrant extract conjugated with ZnO nanoparticle showed that these compounds somewhat protect kidney tissue from diabetes damage . The highest protective effect was observed in diabetic group treated with ZnO nanoparticles conjugated with blackcurrant extract.Conclusion: Our study, for the first time, showed that the conjugation of blackcurrant extract to ZnO nanoparticles can be effective in preventing Renal- tissue damage in diabetic patien
Predictors of intention to quit smoking among hospital male personnel
Get PDFSmoking is the most preventable cause of premature death in the world and its disadvantages impose heavy financial burden on the health system. Among the behavioral models of health education, the most applicable one in smoking cessation is Transtheoretical Model (TTM). This study aimed to detect the predictors of smoking cessation among hospital male personnel. The participants were 200 current and former smokers Personnel working at four hospitals. The instruments included decisional balance scale, self-efficacy scale, processes of change scale (behavioral and experiential), and stages of change which were completed by self-report. Descriptive statistics analysis showed that 66.5% of participants were located in the earlier stages of change. Bivariate spearman correlations showed that self-efficacy, processes of change and decisional balance were significantly correlated with the stage of change. Besides, ordinal regression revealed that experiential processes of change, self-efficacy and behavioral processes were the strongest predictors for the change stages of smoke cessation. The current study revealed that the readiness to quit smoking is significantly predicted by the mechanisms of self-efficacy and the process of change such as environment reevaluation, stimulus control and counter conditioning, which can lead to positive progress in the change stages of smoke cessation. The findings of this study showed that using TTM for smoking cessation would be very effective in improving readiness to quit smoking among hospitals personnel
Comparative Study of Ketofol and Thiopental Sodium in Patients undergoing Dilation and Curettage by General Anesthesia
Get PDFconsidering that the combined drug, Keto fol has been used and its effcacy observed in patients, we have aimed to compare the effects of ketofol and thiopental sodium on patients who are undergoing dilatation and curettage. This study was conducted as a randomized controlled clinical trial. In this study, 150 patients undergoing dilatation and curettage after classifcation were randomly selected and enrolled. The frst group received ketofol, while thiopental sodium was administered to the second group. Any change in he- modynamics or respiratory distress, including the occur- rence of apnea or hypotension, desaturation and the need for respiratory support were recorded in both groups. Fi- nally, after the completion of the surgery and transfer to the recovery room, the recovery rate of each group was recorded, and once they were conscious and alert, were questioned about their satisfaction. All of the patient in- formation was entered into the SPSS v18 software, and the data were analyzed. This study was conducted on 150 patients undergoing curettage; the mean ages in the frst and second groups were 31.82 ± 9.18 and 33.64 ± 11.39 years, respectively. Ninety two percent of patients in the ketofol group and 100% of the patients in the thiopental sodium group experienced apnea and needed respiratory support. About 25.3% of patients in the ketofol group and 44% of patients in the thiopental group required a re-dose of the drug for the anesthetic process. The dura-
tion of recovery in the ketofol group was lower than the
other group. About 2.7% of the patients in the ketofol
group and 12% of the patients in the thiopental group
had hallucinations and delusions at the end of the opera-
tion Six patients in the thiopental group also had hiccups.
The results of the present study showed that ketofol is a
better drug than thiopental because of the duration of an-
esthesia, reducing the need for medication, the incidence
of apnea, and the complications of anesthesi
Scrophularia Atropatana Extract Reverses TP53 Gene Promoter Hypermethylation and Decreases Survivin Antiapoptotic Gene Expression in Breast Cancer Cells
Get PDFBackground: In many cases of breast cancer, the aberrant methylation of TP53 gene leads to uncontrolled cell
proliferation and apoptosis inhibition. Moreover, expression of oncogenes which are under the control of P53 protein
could be altered. Survivin as a conspicuous example of this category plays important roles in tumorigenesis, drug
resistance and apoptosis inhibition. The present study was done to reveal the effects of Scrophularia atropatana extract
on epigenetic situation of TP53 gene promoter and the expression levels of anti-apoptotic gene, survivin and its potential for production of cancer epi-drugs. Methods: Cytotoxic effect of dichloromethane extracts of Scrophularia plant on MCF-7 cell line was assessed in our previous study. Cell death ELISA (enzyme-linked immunosorbent assay) and
TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) tests were used to investigate the occurrence of
apoptosis in the treated cells. Methylation Specific PCR (MSP) was employed to assess the changes in methylation
status of the TP53 gene promoter. Furthermore, quantitative real time PCR was utilized to evaluate the resulting changes
in TP53 and survivin genes expression. Results: Cell death ELISA and TUNEL assays confirmed the occurrence of
apoptosis. MSP test revealed a significant change in the methylation status of TP53 promoter. QRT-PCR showed
an increased TP53 gene expression in the treated cells while a significant decrease in survivin mRNA was evident.
Conclusions: According to the outcomes, dichloromethane extract of S. atropatana returned the TP53 gene promoter
hypermethylation to normal state. This plant could be a promising source for production of epi-drugs due to its apoptotic effects and reversal of TP53 epigenetic alterations
Correlation between keratinized tissue width and periodontal indices around implant-supported fixed partial dentures
Get PDFBackground. The effect of keratinized tissue width on the peri-implant health has not been well elucidated. The results of previous studies on this topic are controversial and the role of keratinized tissue width in the long-term success of dental implants has not been confirmed. This cross-sectional study aimed to assess the correlation of keratinized tissue width with periodontal indices around implant-supported fixed partial dentures (FPDs). Methods. This cross-sectional study evaluated 73 implants. Patients underwent periodontal examinations, including measurement of plaque index (PI), gingival index (GI), clinical probing depth (PD), bleeding on probing (BOP), marginal gingival recession, keratinized mucosa width and radiographic marginal bone level. Data were analyzed using SPSS. Results. The mean GI, PI and marginal gingival recession around implants with 0.05). No significant differences were noted in PD and radiographic marginal bone level between the two implant groups with keratinized tissue width <2 mm and ≥2 mm. Thus, no correlation was found between the keratinized tissue width and the measured indices. Conclusion. Although this study did not show a significant correlation between the keratinized tissue width and peri-implant tissue health and consequently the implant success rate, long-term interventional studies are required to make a final judgment in this respect
Survey of Relationship Between Corruption Perception and Sense of Organizational Justice Among Nurses and Employees of Ardabil Educational Centers In 2017
Get PDFBackground and Aim: Corruption as a phenomenon of economic, political, and cultural misunderstandings is one of the most important and most critical social problems that results in unfair distribution of resources as well as undermining justice and, consequently, the disappearance of justice among citizens. The purpose of this study was to examine the perception of corruption and organizational justice in Between nurses and staff.
Method: In this descriptive cross-sectional study, 426 hospital personnel including nurses and staff in educational hospitals affiliated to Ardabil University of Medical Sciences, were selected randomly and randomly. Data were collected using the organizational justice questionnaire of Niehoff and Moorman and a researcher-made questionnaire on perceived corruption. Data was analyzed by Spss-23 software and correlation, T-test, Kruskal-Wallis and Anova tests and Bonferrini's post hoc test.
The findings of the research showed that the perception of corruption among middle and nursing staff was lower than the average for organizational justice and There was a negative correlation between the perception of corruption with organizational justice, and the distribution of procedural and interactive justice components. (P <0.05). In examining the relationship between demographic characteristics and corruption perception, The components of type of employment, place of service and education were significant with the perception of corruption in nurses and staff (P <0.05). Also, in examining the relationship between demographic characteristics and organizational justice, the relationship between the type of recruitment and place of service and the sense of organizational justice in nurses and staff was significant (P <0.05).
Conclusion: Given the relatively high perceptions of corruption in educational and medical centers, hospital managers need to take necessary and practical measures to reduce perceptions of corruption and increase organizational justice in their respective organizations
Effect of High-fat Diet on Tracheal Responsiveness to Methacholine and Insulin Resistance Index in Ovalbumin-sensitized Male and Female Rats
Get PDFEpidemiological and clinical studies have demonstrated a close association between obesity and asthma. The current study investigated the effect of high-fat diet on tracheal responsiveness to methacholine and insulin resistance in ovalbumin (OVA) sensitized male and female rats. The rats were divided into eight groups (n=6 per group): female with the normal diet (F+ND), male with the normal diet (M+ND), female OVA-sensitized with the normal diet (F+SND), male OVA-sensitized with the normal diet (M+SND), female with high-fat diet (F+HFD), male with high-fat diet (M+HFD), female OVA-sensitized with high-fat diet (F+SHFD), and male OVA-sensitized with high-fat diet (M+SHFD). All rats were fed for 8 weeks with high-fat diet or standard pelts, and for another 4 weeks, they were sensitized with OVA or saline. At the end of the study, the tracheal responsiveness to methacholine, serum insulin, and blood glucose levels was measured. Also, insulin resistance indexes were determined. OVA-sensitization and diet-induced obesity caused the curve of methacholine concentration response to shifting to the left. In addition, results indicated that the EC50 (the effective concentration of methacholine generating 50% of peak response) in F+SHFD rats was statistically lower than M+SHFD group (p<0.05). Moreover, insulin resistance was higher in the F+SHFD than the M+SHFD group (p<0.001). These results suggest that insulin resistance and metabolic syndrome may be involved in the pathogenesis of obesity associated with OVA-sensitized rats condition, especially in female animal
ANALYSIS RISK FACTORS FOR HUMAN CYSTIC ECHINOCOCCOSIS IN MOGHAN PLAIN, AN ENDEMIC REGION OF ARDABIL
Get PDFBackground and Aim:Cystic Hydatidosis (CE) is a Zoonosis and caused by larval stages of Echinococcus granulosus (metacestode) in the intermediate host, eggs of echinococcus released through the feces from infected dogs and eggs ingesting infects humans.Methods:So far no survey was conducted to determine analysis risk factors for the human hydatidosis in Ardabil province, so, using ELISA test and for the first HCF-Ag then Ag-5 and Ag-B and questionnaires forms, the prevalence of this disease was detected in the moghan area in this province. Hospital records defined that cystic echinococcosis is frequent in Moghan Area of Ardabil province. The present study designed to determine the seropositive rate and to analyze risk factors of disease for people living in this region. In this survey, 2680 serum samples were randomly collected from the normal population the everywhere of six shingles of Moghan plain. Sera was storage in -70°C in Ardabil medicine. In the first stage of test for screening the sera was tested using enzyme-linked immunosorbent-assay protocol and HCF-Ag.Results:The serology results + were analyzed by Logistic regression using SPSS 18. Of 2680 serum samples 162 sera (6.9%) and (0.4%), respectively for CE and AE were positive. Women were more than men (21 vs. 11.2%) for CE. The age group of 4-19 showed the lowest rate and the 20-39 and 40-59 showed the highest rate of infection.Conclusion: The rate of prevalence in this province shows somehow a resemblance with the other cities in Iran. Considering the lifestyle in this province a complementary study is suggested in all related cities
Evaluation of moral skills of midwives in delivery room
Get PDFIntroduction: The clinical environment has always faced with ethical challenges. In the delivery room, it is expected midwives to use moral knowledge to solve moral problems. The aim of this study was to investigate the ethical skills of midwifes in delivery rooms in hospitals affiliated to Ardabil University of Medical Sciences.
Methods: This study conducted on 78 midwives in delivery rooms of Alavi and Sabalan hospitals in Ardebil by simple sampling method. Data were collected using demographic questionnaire and Moral Skill Inventory. This questionnaire contains 40 questions in 4 domains (sensitivity, reasoning, integrity and moral courage). The maximum score in this questionnaire is 80 and the minimum score is zero. Data were analyzed by the SPSS18 software using descriptive and analytical statistics methods. The significance level was less than 0.05.
Findings: The mean age of midwives was 31/45 years. Mean score of moral skills among midwives was 58.53±14.05. The highest mean score was for the sensitivity domain 16.08±10.28 and the least mean score was for the reasoning domain 8.82±11.65.
Conclusion: In this study, although midwives' moral skills score is higher than average, midwives score from ethical reasoning is far from ideal level, and further research is needed to improve the ethical reasoning of midwives
Effects of Diet-Induced Obesity on Tracheal Responsiveness to Methacholine, Tracheal Visfatin Level, and Lung Histological Changes in Ovalbumin-Sensitized Female Wistar Rats
Get PDFMany studies have shown a close relationship between obesity and asthma severity. In the present study, the effects of diet-induced obesity were examined on airway responsiveness to methacholine in addition to visfatin level in female Wistar rats’ tracheae after sensitization with ovalbumin. The rats were divided into four groups: control with normal diet (ND), ovalbumin (OVA)-sensitized with normal diet (S + ND), high-fat diet (HFD), and OVA-sensitized with a high-fat diet (S + HFD). The animals were fed for 8 weeks with standard pelts or high-fat diet and then sensitized and challenged with OVA or saline for another 4 weeks. At the end of the study, the tracheae were isolated and assessed for airway responsiveness and visfatin protein levels. Diet-induced obesity groups developed increased weight and obesity indices (p < 0.001). After sensitization with OVA and diet-induced obesity, there were marked leftward shifts in methacholine concentration-response curves in S + HFD group compared to other groups. Also, maximum response was the highest (p < 0.05 to p < 0.001), EC50 was the lowest (p < 0.05 to p < 0.001), and visfatin protein level was the highest (p < 0.05 to p < 0.01) in S + HFD. According to results, diet-induced obesity caused airway hyperresponsiveness to methacholine and enhanced visfatin protein levels in the tracheae of ovalbumin-sensitized female rats. Our results suggested that, in obese ovalbumin-sensitized conditions in female rats, the local production of adipocytokines, such as visfatin, may be increased, resulting in the deterioration of inflammation in lungs. This finding shows a possible mechanism for the altered phenotype in obesity-ovalbumin sensitization conditions in female rats