Ardabil University of Medical Sciences
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Study of the effective factors on HER2 receptor expression and its prognosis in patients with gastric adenocarcinoma in Ardabil province.
No full textBackground and Objective: Gastric cancer is the fourth most common cancer, and is the second leading to death cancer in the world. It seems that the incidence of gastric cancer is significantly different depending on the geographical situation, race, and gender.Common treatments for gastric cancer include surgery, chemotherapy, radiation therapy, or a combination of these. In this research, we try to investigate the presence of HER2 receptor and its role in prognosis of gastric cancer in a population of patients with the help of some laboratory methods. According to the results of studies in several research groups on the excessive expression of the HER2 gene expression, and the increased protein-induced expression of the gene in several cancers, including gastric cancer, as well as the positive effects of the compounds inhibiting the activity of this receptor, Including Trastuzumab - In order to increase the survival rate of the infected, it is important to consider this factor as a selective basis for treatment.
Methods:Seventy patients who referred to Ardabil hospitals who have been diagnosed with gastric cancer by a pathological laboratory have been examined by using the IHC method in terms of HER2 receptor status. Also, information about the age, sex, weight and height of the stage and tumor grade patients were extracted and based on the type of cancer, degree and stage, age, other diseases and other factors, the prognosis of the patient was determined.
Results:The mean age of the participants in this study was 62.13 ± 11.49 years old. Also, in terms of sexual composition, 46 (65.7%) of the patients were men and the rest were women.41 patients(58.57%) were rural and the rest were urban.The history of alcohol and tobacco usage were also studied in subjects with 51 (72.9%) and 5 (7.1%) positive cases respectively. Results showed that 48 (68.6%) patients had lower BMI than normal. HER2 was detected in IHC patients. Results indicated that 11 (15.7%) of subjects were HER2 positive and had a significant correlation between BMI Sex, history of alcohol and smoking, and HER2 expression, and there was also significant relationship between HER2 incidence and age and grade of tumor.
Conclusion : There was a significant relationship between HER2 incidence and age and grade of tumor in patients. These findings can indicate the diagnostic value of this marker in the prognosis of people with gastric acid congestion
Evaluation of the Diagnostic Value of Abdominal Sonographic Measurement of Lower Uterine Segment Thickness in Uterine Rupture during Caesarean Section and Determine its Associated Factors
Get PDFIntroduction:The measurement of the lower uterine segment (LUS) thickness before labor predicts hazardous complications of vaginal birth after cesarean section (VBAC). Vaginal birth after cesarean is one of the most effective methods used to reduce the incidence of cesarean section. Therefore, this study was carried out to evaluate the diagnostic value of sonographic measurement of lower uterine segment (LUS) thickness in uterine rupture during cesarean section and its associated factors in women with and without previous cesarean section.
Methods: This prospective cohort study was conducted among 168 pregnant women in two groups of with and without the history of cesarean section (84 individuals per group) referred to Ardabil Alavi Hospital, Ardabil, Iran, in 2016. The thickness of the LUS was determined through transabdominal ultrasonography between the gestational ages of 36 to 40 weeks. At the time of cesarean section, the thickness of the women’s LUS and newborns’ weight and Apgar score were measured and recorded. Additionally, uterine rupture was recorded in patients with the past history of cesarean section. Moreover, the relationship between the LUS thickness and maternal age, gestational age, the indication for previous cesarean section, interdelivery interval, embryo weight, and abnormalities of erythrocyte were determined. Data analysis was performed using descriptive statistics and t-test in SPSS software, version 16.
Results: According to the pre-operative assessments, the LUS thickness in the women with and without the history of cesarean section were 4.26±1.99 mm and 6.11±1.6 mm, respectively (P˂0.001). According to the findings obtained from sonography before and during the operation, there was a significant difference between the groups in terms of LUS thickness (P0.05). Based on the results, the LUS thickness of 1.8 mm was considered as the critical cutoff value for predicting uterine rupture.
Conclusion: The measurement ofLUS thickness is a non-invasive technique to predict the uterine rupture, which leads to increased rate of VBAC
Awareness assessment of nurses in operating room, ICU, CCU and PICU about radiation protection principles of portable radiography in hospitals of Bandar Abbas, Iran
No full textUtilization of ionizing radiation as portable radiography sometimes is necessary in surgery operations, intensive care units, coronary care units, and pediatric intensive care units that may cause radiation exposure to personnel. The aim of this study was the evaluation of nurses' awareness level about radiation protection principles of the portable radiography in emergency care units. The study was performed on nurses working at two hospitals in Bandar Abbas. For data collection, a researcher-made questionnaire including two sections was used. The first section was related to demographic information of the nurses. The second section included questions related to the nurses' awareness in three fields, including radiation physics, radiation protection, and radiation hazards. Finally, the data were analyzed using the SPSS, version 11.5 software (SPSS Inc., Chicago, IL). The result showed that there was no significant difference between the level of awareness in male and female nurses (p = .565). Also, there was not a significant difference between the nurses' awareness about radiation protection principles with work experience and workplace (p = .393 and .337, respectively). However, there was a significant difference between the nurses' awareness about radiation protection and education level (p = .016). According to the results of this study, nurses' awareness about radiation protection principles is not good, especially among nurses with an associate degree. It seems that their educational level and awareness of potential hazards associated with the use of radiographic examinations need to be increased. To achieve this goal, implementation of periodic training courses for these personnel could be an effective method
Insights into the structural/conformational requirements of cytotoxic oxadiazoles as potential chemotherapeutic target binding agents
Get PDFA few novel previously synthesized 2,5-disubstituted 1,3,4-oxadiazoles with cytotoxic activity (1–17) were subjected to combined docking/quantum mechanical studies against chemotherapeutic targets. Selected macromolecular targets were those that were previously known to be inhibited by 1,3,4-oxadiazoles. Within this work, favorable binding modes/affinities of the oxadiazoles toward validated cancer targets were elucidated. Some oxadiazole structures exhibited ΔGbs comparable to or stronger than crystallographic ligands that were previously demonstrated to inhibit such targets. On the basis of obtained results, a general structure activity/binding relationship (SAR/SBR) was developed and a few 2,5-disubstituted 1,3,4-oxadiazole structures were proposed and virtually validated as potential cytotoxic candidates. To get more insight into structure binding relationship of candidate molecules within best correlated targets, docked conformation of the best in silico in vitro correlated oxadiazole structure was analyzed in terms of intermolecular binding energy components by functional B3LYP in association with split valence basis set using polarization functions (Def2-SVP). We believe that such modeling studies may be complementary to our previous results on the synthesis and cytotoxicity assessment of novel 1,3,4-oxadiazole derivatives through extending the scope of privileged structures toward designing new potential anti-tumor compounds
Effect of Zinc Oxide Nanoparticles on Blood Lipid Profile in Wistar Male Rats
Get PDFIntroduction: Due to the widespread use of zinc oxide nanoparticles (ZnO-NPs) and their possible unwanted effects in biological environments, we have tried to investigate the effect of ZnO-NPs on the blood lipid profile.
Materials and Methods: In this study, 24 male Wistar rats were used. The rats were randomly divided into three groups: control group, the group receiving ZnO-NPs at a dose of 25 mg/kg (ZnO-NPs 25 mg/kg) and the group receiving ZnO-NPs at a dose of 50 mg/kg (ZnO-NPs 50 mg/kg). The duration of the test was two weeks, then blood was taken and the serum was separated. Serum levels of triglyceride, cholesterol, VLDL, LDL and HDL were determined using standard kits and methods.
Results: Based on the findings of this study serum levels of triglyceride and VLDL in ZnO-NPs 25 and 50 mg/kg groups were significantly increased in comparison with the control group (p 0.05).
Conclusion: Our results showed that doses of 25 and 50 mg/kg of ZnO-NPs cause both harmful (increasing of triglyceride and VLDL and HDL reduction) and beneficial (LDL and LDL/HDL ratio reductions) effects in the blood lipid profile
Gender inequality of employed women caused to health complications
Get PDFGender equality means the enjoyment to rights, opportunities, and life chances has not been governed or limited by the gender. One of the issues highlighted in international human rights is the elimination of gender-based discrimination and the need for equality between men and women in the human society. Gender inequality comes from biological differences and social context [1]. Women need different treatment than men to prosperous the same rights. For example, in order to benefit from the right to work, women may need help and support from families and the societyin taking care of the child or doing their homework as a traditional duty
Selective Cytotoxicity of Luteolin and Kaempferol on Cancerous Hepatocytes Obtained from Rat Model of Hepatocellular Carcinoma: Involvement of ROS-Mediated Mitochondrial Targeting
No full textTo evaluate the cytotoxicity effects of luteolin (LUT) and kaempferol (KAE) via reactive oxygen species (ROS) mediated mitochondrial targeting on hepatocytes obtained from the liver of hepatocellular carcinoma (HCC) rats. In this study, HCC induced by diethylnitrosamine (DEN) and 2-acetylaminofluorene (2-AAF). In the following, rat liver hepatocytes and mitochondria were isolated and tested for every eventual apoptotic and anti-HCC effects of LUT and KAE. The results of MTT assay showed that LUT and KAE were able to induce selective cytotoxicity in hepatocytes of HCC group in a dose- and time-dependent manner. Treatment of mitochondria from hepatocytes of HCC group with LUT and KAE were accompanied by loss of mitochondrial membrane potential (MMP) and mitochondrial swelling and release of cytochrome c ( P < 0.001) via reactive oxygen species (ROS) generation before cytotoxicity ensued. LUT and KAE also increased activation of caspase-3 (P < 0.001 andP < 0.01, respectively). Flow-cytometry analysis indicated that the mode of cell death induced by these fl avonoids were mostly apoptosis. Importantly, LUT and KAE were nontoxic for healthy hepatocytes and mitochondria. Therefore, we suggest that LUT and KAE are a good candidate for the complementary therapeutic agent against HCC
Enhancement of methylene blue adsorption onto activated carbon prepared from Date Press Cake by low frequency ultrasound
No full textThis study was aimed to evaluate the adsorption of methylene blue onto activated carbon prepared from Date Press Cake (AC-DPC) in the absence (stirring) and presence of low frequency ultrasound (no stirring). Date Press Cake (DPC) was converted into activated carbon (BET surface area of 2632.5 m2 g−1) using solid KOH. Batch adsorption experiments illustrated that the adsorption of MB onto AC-DPC was more favored at alkaline conditions. Kinetics analysis showed that the adsorption of MB onto AC-DPC was controlled by film diffusion in both agitation methods. The values of adsorption rate constants in kinetic models were increased by applying low frequency ultrasound to the adsorption system. Such acceleration effect was reflected in equilibrium contact times, which were 180 and 20 min in the absence and presence of ultrasound, respectively. The experimental equilibrium data for both agitation methods were best fitted with Redlich-Peterson isotherm model, indicating chemisorption nature of MB adsorption onto AC-DPC. The maximum monolayer adsorption capacity of MB on AC-DPC was found to be increased from 546.8 mg g−1 (stirring) to 613.8 mg g−1 in the presence of low frequency ultrasound. Therefore, low frequency ultrasound increased both the rate and amount of MB adsorption onto AC-DPC. Overall, KOH-activated carbon prepared from Date Press Cake efficiently adsorbed MB from aqueous solutions. Moreover, the equilibrium and kinetics of MB adsorption onto AC-DPC could be improved in the presence of low frequency ultrasound
Protective effects of the hydroalcoholic extract of Fumaria parviflora on testicular injury induced by torsion/detorsion in adult rats
No full textThis study was designed to determine the effects of daily oral administration (250 mg/kg) of the hydroalcoholic extract of Fumaria parviflora (FP) for 14 days on the sperm parameters, oxidative stress parameters, serum testosterone levels, expression of Bax and Bcl‐2 genes, and apoptosis index of germ cells after testicular torsion–detorsion (ischaemia–reperfusion, IR) injury model in rats. Twenty‐eight adult male Wistar rats were divided randomly into four groups of seven each: sham operation, torsion–detorsion (TD), TD plus the hydroalcoholic extract FP (TDFP) and only FP without TD application (FP). Testicular torsion was created by rotating the left testis 720° in a counterclockwise direction; then, after 4 hr, detorsion was performed. The Johnson's score, mean seminiferous tubule diameter (MSTD) and height (thickness) of seminiferous tubule epithelium (HST) were significantly increased in TDFP and FP groups as compared to TD group. The gene expression of Bcl‐2, level of serum testosterone hormone and antioxidant parameters—GPx and SOD—were significantly higher in TDFP and FP groups than TD group. The index of apoptosis, the gene expression of Bax and the level of MDA were significantly higher in TD group than TDFP and FP groups. Therefore, F. parviflora could decrease oxidative stress induced by testicular torsion–detorsion
Measurement of Mitochondrial Toxicity Parameters in Embryonic Hippocampus
Get PDFRecent discoveries have focused on mitochondria functions in the neuroscience research for approaches to study mitochondria dysfunction in neurodegenerative diseases. Mitochondrion is one of the organelles that is possibly worst affected in cognitive impairments. These are known as “powerhouse” of the cell as they are the main source of generation of ATP through aerobic respiration. They have role in oxidative phosphorylation and metabolism, they play central role in cell differentiation, apoptosis, oxygen sensing and detoxification of reactive oxygen species, innate immunity, mitochondrial matrix calcium, and maintenance of cell quality and regulation of cytoplasmic. There is a relationship between mitochondrial dysfunction and cognitive disorder that may be related to certain neurotoxins or mutations in mitochondrial DNA as well as the nuclear. Evaluating compounds for mitochondrial toxicity is an important capability for evaluation of cognitive effects by drugs. Studying mitochondria isolated from individual mouse brain regions is a challenge because of small amount of the available brain tissue. There are conventional techniques for isolation and purification of mitochondria from ventral midbrain, hippocampus, or striatum.
The utilization of alcohol within pregnancy impairs the development of the unborn offspring and can lead to a plethora of anatomical, behavioral, and cognitive abnormalities. In here, a method for isolation of brain mitochondria from mouse is described. The method utilizes a refrigerated tabletop microtube centrifuge, and produces research grade quality mitochondria in amounts sufficient for performing multiple enzymatic and functional assays, thereby eliminating the necessity for pooling mouse brain tissue. A method for measuring ROS measurement, mitochondrial membrane potential, mitochondrial swelling, cytochrome c release, and mtDNA alterations after exposure to drugs is also
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