Ardabil University of Medical Sciences
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Germ cell differentiation of bone marrow mesenchymal stem cells.
No full textBone marrow mesenchymal stem cells (BM‐MSCs) were first cultured under induction of retinoic acid (RA), Sertoli cells conditioned medium and RA + con (conditioned medium) as treatment groups. The presence of Sertoli cells was confirmed by immunocytochemistry of follicle‐stimulating hormone receptor in Sertoli cells and flow cytometry by anti‐Gata4 antibody. Cell viability and morphology of nucleus and cytoplasm of BM‐MSCs were evaluated by MTT test and DAPI staining respectively. The expression of Oct4, Plzf, Scp3, Caspases 8, 9 and 3 genes was evaluated by RT‐PCR. For increasing the accuracy of experiment, the expression of Vasa and SCP3 genes was investigated quantitatively by real‐time PCR after 0, 5, 10, 15 days of culture. The results showed that the number of apoptotic cells increased in RA group. The expression of apoptosis genes (Caspases 3, 8 and 9) was also observed in this group all days of culture. Measurement of Vasa and Scp3 genes by RT‐PCR confirmed the positive effects of retinoic acid on increasing of genes expression. So, in this study, a group with maximum expression of differentiation genes and minimum expression of apoptotic genes was RA + conditioned medium group. DNA fragmentation was not observed in all groups
Anti Nuclear Anti Body (Ana) in Visceral Leishmaniasis
Get PDFBackground: The ANA test is a blood test that is commonly used for evaluating of autoimmune or connective tissue disorders. Visceral leishmaniasis is a chronic infectious diseases wich the cellular Immune mechanisms responsible for the control of leishmania infections.This study attempted to investigate the ANA in leishmaniasis, since there was a probability of observing highly positive ANA in this disease. methods: In this study, we collected samples of blood of children who were infected by visceral leishmaniasis .The number of samples was 26 and was collected from three hygienic centers in Ardebil, Meshkin and Germi within 1.5 years. In one sample the DAT of serum was negative, but in the biopsy of bone marrow the leishman's body was reported. The participants were 9 (34.6%) females and 17 (65.4%) males and their ages varied from 5 months to 5 years (mean 21 months). Results: in the 11 cases (42.3%) the ANA test was positive and in 15 cases (57.7%) was negative. The most frequent of positive ANA titers in 2 cases (7.7%) was 14 and the highest titer was 50. ANA in 5 cases of females (55.6%) and in 6 cases of males (35.6%) was positive. Conclusion study indicates that visceral leishmaniasis is an infectious disease
which in the antinuclear antibody (ANA) test can be positive. Presence of positive ANA test in visceral leishmaniasis probably be due to disease pathogenesis and changes in immune system and it's disorders
Evaluating diagnostic value of clinical symptoms and signs for chronic sinusitis by CT-scan in patients admitted to Ardabil city hospital, Iran
Get PDFBackground: Chronic rhinosinusitis (CRS) refers to inflammatory nasal and para nasal sinuses which last at least 12 weeks. Despite its high prevalence, it is not easy to diagnose, and clinical criteria and simple radiographs are not so sensitive. The aim of this study was to investigate the diagnostic value of clinical symptoms and signs for chronic sinusitis by Ct-scan in patients admitted to Ardabil city hospital, Iran.
Methods: This descriptive cross-sectional study has been done on 83 patients with CRS symptoms who referred to ENT of Ardabil city hospital. The necessary checklist included information such as age, gender, clinical symptoms and CT scan findings were completed and analyzed by statistical methods in SPSS version 21.
Results: Of all 83 patients, 55.4% were female and most of patients were in age group 30-39 years with 30.1%. Of all patients, 29 (35%) had positive findings in CT scan of which 72.4% were female. The highest rate of sinus involvement was in the ethmoid sinus. The highest positive predicted value was for nose congestion with OR=2.26 and the lowest was for facial pain and toothache each with OR=0.1.
Conclusions: In general, clinical symptoms have a small predictive value in the diagnosis of sinusitis and should be evaluated with CT scan findings (15.7%)
Kawasaki disease and familial mediterranean fever gene mutations, is there any link
Get PDFBackground and aim: Kawasaki disease (KD) is an acute febrile, self-limiting, and systemic vasculitis of unknown etiology. MEFV gene has a major role in autoinflammatory disorders and innate immune reactions. Several reports revealed that MEFV gene mutations are associated with systemic vasculitis. The aim of this study was to determine the association between KD and MEFV gene mutations.
Methods: The peripheral blood of 30 patients who were diagnosed with KD based on ACC criteria and 224 healthy people as a control group (113 male and 111 female), were collected and the samples screened for the 12 common pathogenic variants according to manufacturer’s instructions.
Results: The mean age of patients (13 females and 17 males) was 7.7 years. Ten percent of patients showed a mutation, that was meaningfully (p<0.05%) lower than that of healthy controls (25%). E148Q was shown in two patients and compound heterozygous (E148Q-M680I) was detected in one of them with lack of FMF presentations. No significant and meaningful associations were detected between the MEFV gene variant alleles and KD.
Conclusion: Unlike in other types of pediatric vasculitis, this study did not reveal any significant association between the MEFV gene mutations and KD, moreover, because of the lower frequency of mutations in these patients, it seems that this gene has a modifier and/or protective role in KD
Evaluation of dimensional changes and detail reproduction of three type IV dental gypsums
Get PDFBackground and Aims: Gypsum products are used for several different purposes. Adaptation and success of
casting restorations are dependent on dimensional accuracy, strength, and reproduction of details of dental gypsums.
This study was designed to evaluate the dimensional changes and accurate details reproduction of three type IV
dental gypsums.
Materials and Methods: 60 samples in 3 groups of dental gypsums of G30,Yeti Rock and FujirockEP were
evaluated. The detail reproduction of 50 micron scratched line was analyzed by a microscope at 12× magnification.
The samples were assayed for dimensional changes at 2 and 24 hours and 1 week after pouring. Photograph images
were taken from samples in fixed distance by a camera (Canon D600) and analyzed in corel draw software. Data
were analyzed using ANOVA and chi-square (P<0.05).
Results: There were significant differences in the details reproduction between three gypsums (P≤0.05) and the
highest values was for Fujirock EP, Yeti Rock. The G30 did not reconstruct the details. There was no significant
difference in the dimensional changes of the evaluated gypsums based on time (P≥0.05).
Conclusion: All three different gypsums evaluated in this study showed the dimension
Study of marital adjustment in patients with laminectomy referred to Fatemi hospital on Ardabil in 1397
No full textBackground and Objective:. Chronic low back pain can be one of the reasons for reducing the amount of marital adjustment. In this study, the degree of marital adjustment before and after laminectomy surgery, and the rate of increase or decrease of its possible causes were investigated.
Methods: The sample size was 72 patients who were candidated for laminectomy. Patients completed the International Standard Marital Adjustment (DAS) questionnaire two times; once before surgery and once 3 months after surgery. Datas were entered into SPSS software and analyzed using Pearson correlation and T-test.
Results: The results of our study showed that marital adjustment after laminectomy surgery in all its dimensions includes marital satisfaction, correlation, affection and mutual trust increased. This increase also showed a statistically significant relationship (P <0.05). It was also concluded that in terms of educational level, the lowest levels of education showed a higher degree of marital adjustment, and the lowest level of adjustment was observed in retired patients.
Conclusion: Laminectomy surgery in patients with chronic low back pain and surgical candidates can increase marital adjustment in all its dimensions
In vitro comparison of antimicrobial effects of varnish fluoride's with or without CPP_ACP
Get PDFIntroduction: Tooth decay, multifactorial and infectious oral disease are infectious that is primarily caused by the complex interaction of biofilm (decaying oral flora) with dietary fermentable carbohydrates on the tooth surface over the time. This thesis aimed to investigate the in vitro comparison of the antimicrobial property of fluoride varnishes containing CPP_ACP or without CPP_ACP.
Materials and Methods: In this in vitro study, we compared the sensitivity effect of Streptococcus mutans to the varnish of MI Varnish, Centrix Varnish, Voco Varnish and V Varnish. For this purpose, Streptococcus mutans was prepared as lyophilized vials from Iranian Scientific and Industrial Research Center; disk diffusion test and bacterial killing curve were performed to evaluate the antibacterial activity of the materials; at least 3 observations (lethality, halo diameter) were made for each varnish and based on each output and 24 observations (12 varnish based on lethality 2and 12 halo diameter observations) were performed, and comparisons were made between observations within each varnish as well as between varnishes. Also, to study the bacterial lethality of the varnishes, optical absorption at 570 nm was read by ELISA reader (BioTek, Winooski, VS) and was analyzed by intergroup ANOVA and repeated measurement analysis tests with 5% error in Spss software version 18.
Results: The results of this study showed that there was no significant difference in diameter of bacterial growth halo and light absorption between varnish MI and Centrix (P> 0.05). Also, no significant difference was observed in diameter of bacterial growth halo and optical absorption between V varnish and Voco varnish (P> 0.05), but significant difference in diameter of bacterial growth halo and optical absorption was observed individually in MI varnish and Centrix varnish with V varnish and Voco varnish (P <0.05).
Conclusion: According to the results of this study and previous studies, it seems that fluoride varnish containing CPP-ACP (MI Varnish) and Centrix varnish have stronger antimicrobial activity than CPP-ACP free (V and Voco varnish) fluoride varnis
An investigation of Factors Related to Aggression in Clients Referring to Ardebil Forensic Medicine Center in 1398
No full textBackground and objective : Given the increasing violence in Iranian society, and especially Ardabil, which ranks second in terms of the prevalence of street fighting in the country and the personal and social harms of violence, the high cost to the individual and society, the increase in family damages and divorce, Increasing the number of inmates, homicide and crime in the community, this study aimed to investigate the factors associated with aggressive behavior that were identified and documented in the Ardabil Forensic Center.
Methods : In this study, the existing data were obtained from the files registered in Ardabil Forensic Medical Center in 1398. In the first step, based on the objectives of the study and specialists opinion, the data collection table was formulated as a checklist, and in the second step, the information required for each individual, including age, sex, level of socioeconomic, education, psychological and family problems were obtained from existing files in Ardabil Forensic Center.
Results: In this study, 68 aggression cases referred to forensic medicine in Ardabil city were studied. Of these, 70.6% (48 cases) were males and 29.4% (20 cases) females. People ranged in age from 17 to 68 years. The mean age of the participants was 35.18 ± 13.14 years. The majority (30.9%) had a diploma degree, the majority(61.8%) were married and the rest (38.2) were single. 55.9% (38 cases) of the employed and the rest (44.1%) were unemployed. Most (76.5%) were urban and the rest were rural. It was also found that poor economic status with 60.3% (41 cases) had the highest frequency, followed by moderate (29.5%) and good (7.4%) with 5.9% (4 cases), respectively. 5.9% (4 cases) had a history of mental illness. 13.2% (9 cases) reported financial problems and 14.7% (10 cases) reported family problems, 4.4% (3 cases) reported death of parents in the last year. 4 cases (5.9%) had a history of anger and violence, and 1 case (1.5%) reported history of suicide. On the subject of cause of aggression 11 cases were unknown. The most common cause of aggression was insulting with 14 cases (24.6%), followed by financial problems with 13 cases (22.8%) and honor issues with 12 cases (21.1%).
Conclusion : Considering that in the this study most of the people referring to forensic medicine were young, male and city resident, in the event of serious physical injuries it can cause socioeconomic costs as a result of the loss of active labor force in the community. Given that most of the violence has taken place in urban areas with poor economic status, planning for job creation for young individuals and life skills training for families can reduce stress, reduce aggression, and then reduce violence in the society
A comparison of biofiltration performance based on bacteria and fungi for treating toluene vapors from airflow
Get PDFWith increasing concerns about industrial gas contaminants and the growing demand for durable and sustainable
technologies, attentions have been gradually shifted to biological air pollution controls. The ability of Pseudomonas
putida PTCC 1694 (bacteria) and Pleurotus ostreatus IRAN 1781C (fungus) to treat contaminated gas stream with
toluene and its biological degradation was compared under similar operating conditions. For this purpose, a biofilter
on the laboratory scale was designed and constructed and the tests were carried out in two stages. The first stage,
bacterial testing, lasted 20 days and the second stage, fungal testing, lasted 16 days. Inlet loading rates (IL) for bacterial
and fungal biofilters were 21.62 ± 6.04 and 26.24 ± 7.35 g/m3 h respectively. In general, fungal biofilter showed a
higher elimination capacity (EC) than bacterial biofilter (18.1 ± 6.98 vs 13.7 ± 4.7 g/m3 h). However, the pressure drop
in the fungal biofilter was higher than the bacterial biofilter (1.26 ± 0.3 vs 1 ± 0.3 mm water), which was probably due
to the growth of the mycelium. Fungal biofiltration showed a better performance in the removal of toluene from the
air stream.
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