Ardabil University of Medical Sciences
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Management of celiac trunk and superior mesenteric artery synchronous aneurysms as an extremely rare manifestation of Wegener granulomatosis
No full textLarge-vessel aneurysm is an extremely rare complication of Wegener granulomatosis. We report a case of Wegener
granulomatosis in a 49-year-old woman with large synchronous aneurysms of the celiac trunk (54 mm) and superior
mesenteric artery (42 mm) who presented with abdominal pain. Because of the large diameter of the aneurysms and
their proximity to each other, a combination of endovascular and hybrid repair was used for management. After surgical
debranching and endovascular repair, the patient was discharged in good general condition. We concluded that
abdominal pain in Wegener granulomatosis can be a rare manifestation of a large visceral aneurys
Azelaic acid stimulates catalase activation and promotes hair growth through upregulation of Gli1 and Gli2 mRNA and Shh protein
Get PDFObjective: Although azelaic acid is effective for treatment of acne and rosacea, the biological activity of azelaic acid and the effect of its combination therapy with minoxidil were not elucidated with regard to hair growth.
Materials and Methods: In this study, mouse vibrissae follicles were dissected on day 10 after depilation. Then, the bulb and bulge cells of the hair follicle were treated with minoxidil and azelaic acid for 10 days to evaluate Sonic hedgehog (Shh) protein expression. Moreover, bulge and bulb cells of the hair follicles were cultivated and the expression of Gli1, Gli2, and Axin2 mRNA levels was evaluated using real-time polymerase chain reaction (PCR) analysis. We further investigated the protective effects of azelaic acid against ultraviolet B (UVB) irradiation in cultured bulb and bulge cells by determining catalase activity. An irradiation dose of 20 mJ/cm2 UVB for 4 sec was chosen.
Results: The results showed that catalase activity significantly (p<0.05) increased in the bulge cells after exposure to 2.5 mM and 25 mM azelaic acid. Meanwhile, treatment of the bulb cells with azelaic acid (2.5 and 25 mM) did not cause significant changes in catalase activity. We also found that azelaic acid (25 mM) alone upregulated Gli1 and Gli2 expression in the bulge cells and 100 µ minoxidil caused Gli1 and Axin2 overexpression in the bulb region of the hair follicle. Moreover, minoxidil (100 µM) alone and in combination with azelaic acid (25 mM) led to Shh protein overexpression in the hair follicles in vitro and in organ culture.
Conclusion: Our results indicated a potential role for azelaic acid in the protection of bulge cells from UVB damage and its combination with minoxidil may activate hair growth through overexpression of Shh protein
Synthesis of Asparaginase-conjugated Trastuzumab monoclonal antibody and study of its cytotoxic effect on gastric cancer cell line (AGS)
No full textIntroduction
According to global statistics, cancer is one of the most important causes of death in the world. Among the known cancers, gastric cancer is one of the most deadly. Due to the high population, Asia has the highest incidence of gastric cancer and the highest mortality rate. Iran is also one of the areas where the risk of gastric cancer is high, and the World Health Organization report points to these. According to the Iranian Ministry of Health and Medical Education, due to the high cost and fatality of gastric cancer, this cancer has particular importance in Ardabil province. Existing methods and current chemotherapy drugs have not achieved satisfactory success in the treatment of gastric cancer patients and finding new drugs with high efficacy and low side effects seems unavoidable. To achieve higher efficacy and lower side effects, we conjugated the drug aspartaginase with the monoclonal antibody trastuzumab and examined its effects on gastric cancer cells
materials and methods
In the first step, the trastuzumab monoclonal antibody was activated by the 3,3′-dihydro-propionic acid (N-hydroxysuccinimide ester) (DSP) disulfide linker to link the end of lysine amino acid to the linker. The asparaginase enzyme was then added to it with a molar ratio of antibody. The mixture was kept at refrigerator temperature for 48 hours and then dialysed to purify the unreacted material. Electrophoresis and dynamic light scattering were used to prove correct binding. To investigate the effects of cellular toxicity and apoptosis, acridine orange and ethidium bromide staining were used.
Results
Using polyacrylamide electrophoresis in the presence of sodium dodecyl sulfate, antibody conjugation with asparaginase was demonstrated, and dynamic light scattering showed increase in size and surface charge change after monoclonal antibody conjugation with asparaginase. The results of Acridine Orange and Ethidium Bromide staining with fluorescent microscope for apoptosis studies showed that those cells treated with trastuzumab-asparaginase conjugate showed higher apoptosis than the asparaginase alone group,trastuzumab alone group And control group.
Conclusion
Our studies showed that binding of monoclonal trastuzumab antibody to the asparaginase enzyme using disulfide linker enhances the apoptotic effect. However, these studies were performed on cultured cells and qualitively evaluated the amount of apoptosis induced by the conjugate. Subsequent studies will quantitatively evaluate the cell death caused by the conjugate in animal studies
Study of toxicity of bevacizumab in mitochondria isolated from rat heart and study of curcumin's role in inhibiting its toxicity
No full textBevacizumab, the first inhibitor of the vascular endothelial growth factor (VEGF), has become one of the best-selling drugs in the world. Heart failure has been seen in 2–4% of patients on bevacizumab. Mitochondria play an important role in myocardial tissue homeostasis and deterioration in mitochondrial function will eventually lead to cardiomyocyte cell death and consequently cardiovascular dysfunction.
Materials and Method :
The aim of our study is to search the effects of bevacizumab on isolated rat heart mitochondria and survey the effect of curcumin as a mitochondrial protective agent and cardioprotective. Rat heart mitochondria were isolated with mechanical lysis and differential centrifugation. Using by biochemical and flowcytometry evaluations, the parameters of mitochondrial toxicity including: succinate dehydrogenase (SDH) activity, mitochondrial swelling, mitochondrial membrane potential (MMP) collapse, reactive oxygen species (ROS) formation and lipid peroxidation (LP) was evaluated.
Results :
Results revealed that bevacizumab (up to 50 μg/ml) induced a concentration- and time-dependent rise in mitochondrial ROS formation, MMP collapse, mitochondrial swelling, lipid peroxidation and inhibition of succinate dehydrogenase in rat heart mitochondria. Our results showed that curcumin (10-100 μM) significantly ameliorated bevacizumab-induced mitochondrial toxicities.
Discussion :
These results indicate that the cardiotoxic effects of bevacizumab are associated with mitochondrial dysfunction and ROS formation, which finally ends in MMP collapse and mitochondrial swelling as the “point of no return” in the cascade of events leading to apoptosis. Also, results of this study suggest that the combination therapy of bevacizumab with curcumin could decrease mitochondrial effects of this drug
Evaluation of the clinical education experiences from medical students of view in Ardabil University of Medical Sciences: a qualitative study
No full textBackground and Objective: Ensuring the quality of education is one of the responsibilities of medical instructors. Understanding the educational issues related to courses to improve the quality of medical education. The purpose of this study was to evaluation the clinical education experiences from medical students of view in Ardabil University of medical sciences.
Methods: A qualitative design using a conventional content analysis approach was used to collect and analyze data. Semi structured interviews were conducted with 12 medical students chosen using purposeful sampling.
Results: In this study, 7 cases (58.3%) were male and the 5 cases (41.7%) were female. The mean age of students was 27.75±1.83 years with a range of 33- 26 years. During data analysis, categories were developed: “ineffective instructor”, “non-dynamic curriculum”, “problems regard to external environment of education”, and “factors related to the individual student”.
Conclusion: It was shown that education issues are caused by different factors. It is suggested that educational administrators should provide educational facilities and help with the improvement of instructors' teaching and communication skills in order to take effective steps in removing educational issues, and enhance students' motivation and confidence
Evaluation Of Factors Related To Adherence To Drug Therapy In Patients With Bipolar I Disorder Referred To Psychiatric Clinic Of Fatemi Hospital In Ardabil In 1398
Get PDFBackground and Objective: Non-adherence with drug therapy is an important obstacle to treatment effectiveness. Understanding the causes of non-adherence to medication has play a major role in solve this problem. The results of this study can help psychiatrists in managing their patients and choosing better educational methods for better adherence of patients.
Methods:. The present study was a cross-sectional observational study conducted in the first and second half of 1398 in the psychiatric clinic of Dr. Fatemi Hospital of Ardabil University of Medical Sciences. In this study, 93 patients with type 1 bipolar disorder referred to Fatemi Hospital psychiatric clinic were studied. . Sampling in this study is available sampling. patients After explaining and obtaining written informed consent to participate in the study, the inclusion and exclusion criteria were included in the study. Data were collected through a Morriskey and demographic questionnaire and interviewed individually.
Results: Our findings showed no significant relationship between age, treatment (number of medications, years of drug use), education, forgetting, drug side effects, drug efficacy, and trust in physician (p <0.05). . There was a significant relationship between gender of patients, belief in medication treatment of patients' place of residence and access to medication and income of patients with adherence to medication. Studies have shown that believing in and accepting the illness and taking the illness seriously, as well as the family's involvement in the treatment, increase patients' adherence to medication. (P <0.05) Our findings showed that marital status play an important role in adherence to treatment, so adherence in married patients is significantly higher than single patients. (P<0.05)
Conclusion: Identifying the factors affecting adherence to drug therapy can pave the way for greater adherence to patients so that additional costs due to non-adherence to drug treatment are not imposed on patients and their patients' families and society, and better patient outcomes are achieved.
The prevalence of familial multiple sclerosis (FMS) in Ardabil province, Ardabil, Iran
Get PDFIntroduction: Multiple sclerosis is one of the most common demyelinating diseases of the central nerves system that occurs frequently in families. The aim of this study was to investigate the prevalence of familial multiple sclerosis in Ardabil province, Ardabil, Iran.
Methods: This cross-sectional study was performed on 611 patients who registered in Ardabil MS registry system in Alavi hospital. The necessary data such as demographic data and having a relative with MS, degree of relatives, residence place, number of hospitalization, signs and symptoms of disease collected by a checklist and then analyzed by statistical methods in SPSS version 19. The p<0.05 was considered as significant.
Results: Of all patients, 440 (72%) were female and rest of them were male with ratio 2.57. Of all patients, 75.5% were married, 66.8% were from Ardabil city, 30.8% employee and 35.4% had education in level Diploma and low. The most symptoms in patients were sensory impairment with 14.2%. In this study 85 (14%) of MS patients had familial history of MS in their family that of them in 38.8% was in first degree relative.
Conclusion: Results showed that the FMS in Ardabil was lower than other provinces in country Iran
Calcitriol protects the Blood-Brain Barrier integrity against ischemic stroke and reduces vasogenic brain edema via antioxidant and antiapoptotic actions in rats
No full textBackground
Vasogenic brain edema is the most important complication of ischemic stroke that aggravates primary brain injury. Ischemia-Reperfusion (IR)-induced Blood-Brain Barrier (BBB) impairment limits the use of recombinant tissue plasminogen activator (r-tPA) by increasing the possibility of hemorrhagic transformation and contributing to vasogenic edema and neuroinflammation. This study examined the effects of post-ischemic treatment with calcitriol on cerebral infarction, vasogenic edema formation and BBB disruption in a rat model of ischemic stroke.
Methods
Male Sprague-Dawley rats were divided into three main groups, including the sham, IR + vehicle and IR + calcitriol groups. Transient focal cerebral ischemia was induced by a 60-min-long occlusion of the left middle cerebral artery. The infarct volume, brain edema, BBB permeability and antioxidant enzyme activities were evaluated 24 h after ischemia. Immunohistochemical analysis was conducted to investigate cell apoptosis and Brain-Derived Neurotrophic Factor (BDNF) protein expression five days after ischemia.
Results
Compared to the IR + vehicle group, the IR + calcitriol group showed a reduced brain infarction volume, attenuated brain edema formation and improved BBB function. These protective effects were followed by the upregulation of antioxidant enzyme activities in the brain tissue. Additionally, a diminished cell apoptosis and an increased BDNF immunoreactivity were obtained in the IR + calcitriol group.
Conclusion
Calcitriol may reduce brain injury and attenuate vasogenic edema by upregulating antioxidant enzymes activities, reducing cell apoptosis and increasing BDNF protein in the brain tissue in a rat model of ischemic stroke
Analgesic effect of methanolic extracts of Truffle (Terfezia boudieri) in the Cuprizone-Induced Experimental MS in female rats
Get PDFBackground and Objective: Multiple Sclerosis (MS) is one of the inflammatory diseases that cause CNS invasion and loss of neurological function. It is the most common neurological disease in the world that affects people from a young age. Pain is one of the most common symptoms of MS. The prevalence of pain among MS patients is estimated to between 30 to 86%. The aim of this study was to investigate the hydroalcoholic effects of Terfezia boudieri on pain relief in MS patients by developing a MS model in female mouses.
Methods: In this study, 35 adult Wistar rats by weight 30 g were enrolled. The rats were divided into 5 groups of 7 groups each. Normal control group received no extract. The MS group consisted of rats that received 40 days of oral coprizon (water soluble) toxin for 40 days at 0.1gr / kg. The 250 mg / kg extract group received MS and 250 mg / kg extract. The 500 mg / kg MS extract group received 500 mg / kg extract. The 750 mg / kg MS extract group received MS. And 750 mg / kg extract. Cuprizone was administered orally (water soluble) daily for 40 days at 0.1 mg / kg as a toxin to form the MS model. Pain tolerance test in groups was performed by hot-plate and tail-flick apparatus.
Results: Coprizone toxin caused MS symptoms in mice and significantly decreased weight in mice. Terfezia boudieri extract at 500 and 750 mg doses was effective in restoring the weight of the rats. In the delayed time to pain response to assess the antinociceptive effect of the extract, the MS group was significantly reduced and the time difference The MS group with the control group and the extract group with 750 mg had a significant difference with the MS group. Use of Danbolan fungus extract at 750 mg dose significantly prevented superoxide dismutase activity
Conclusion: It seems that Terfezia boudieri extract has beneficial effects in the control of MS