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HUBUNGAN ANTARA PERSEPSI DAN TINGKAH LAKU PENGGUNAAN PRODUK AYAM BAKAR DALAM KALANGAN BELIA MALAYSIA KE ARAH KUALITI HIDUP YANG LEBIH BAIK, DI KUALA LUMPUR, MALAYSIA
No full textTranscriptomics in advancing portunid aquaculture: A systematic review
No full textThe rapid advancement of next-generation sequencing technologies has promoted
the use of transcriptomics in non-model organisms, including species in the aquaculture
sector. Compared to that of other crustacean species, portunid crab aquaculture
is impeded by the insufficient knowledge of their biological and physiological processes.
This systematic review summarised the advances in transcriptomics in cultured
portunid crabs using a systematic literature review methodology. The filtered
transcriptome dataset comprised 66 articles from four genera: Scylla, Portunus, Callinectes
and Charybdis. At the species level, Scylla paramamosain and Portunus trituberculatus
were the two most studied species, highlighting their importance in the
aquaculture sector. The affordable cost of RNA sequencing (RNA-Seq) led to an
increase in transcriptome-related research in portunid crabs and made available a
huge repertoire of differentially expressed genes (DEGs) and single nucleotide polymorphisms
(SNPs). We further discussed the transcriptomic advances based on six
main functional categories, that is, ?growth and moulting?, ?gonadal development and
reproduction?, ?nutrition metabolism?, ?disease and immunity?, ?toxicology and stress?
and ?general transcriptomic profiling?. In general, transcriptomic studies of portunid
crabs, specifically the DEGs and pathways allow an in-depth understanding of the
biological and physiological processes involved at different growth stages or under
various conditions based on the difference in gene transcriptional activity. SNPs
obtained from the transcriptome data are useful in many genetic improvementrelated
downstream applications, including the construction of genetic linkage maps
and as population genetic markers. Future directions, such as hybrid approaches of
long-read and common RNA-Seq, and the incorporation of other omics were also
discussed
Quorum sensing signals: Aquaculture risk factor
No full textBacteria produce several virulence factors and cause massive mortality in fish and
crustaceans. Abundant quorum sensing (QS) signals and high cell density are essentially
required for the production of such virulence factors. Although several strategies
have been developed to control aquatic pathogens through antibiotics and QS
inhibition, the impact of pre-existing QS signals in the aquatic environment has been
overlooked. QS signals cause detrimental effects on mammalian cells and induce cell
death by interfering with multiple cellular pathways. Moreover, QS signals not only
function as a messenger, but also annihilate the functions of the host immune system
which implies that QS signals should be designated as a major virulence factor.
Despite QS signals' role has been well documented in mammalian cells, their impact
on aquatic organisms is still at the budding stage. However, many aquatic organisms
produce enzymes that degrade and detoxify such QS signals. In addition, physical and
chemical factors also determine the stability of the QS signals in the aqueous environment.
The balance between QS signals and existing QS signals degrading factors
essentially determines the disease progression in aquatic organisms. In this review,
we highlight the impact of QS signals on aquatic organisms and further discussed
potential alternative strategies to control disease progression
After the collapse: Evaluating undone science in the wake of a global environmental crisis
No full textThis study deepens our understanding of environmental knowledge production under varying political-economic regimes. While the extant scholarship on undone science, that is the systemic non-production of knowledge, is largely based in liberal democracies in the US and Europe, it elides important differences in the making and unmaking of science elsewhere. Using the case of Peru?s left-leaning military dictatorship (1968?1980), I show how undone science contributed to the 1973 collapse of the world?s largest fisheryand one of the most studied fisherieson the planet: the anchoveta (Engraulis ringens). In an effort to reconstruct the fisheryand resolve un- done science, I also demonstrate how the state collapsed established boundaries by brokering research between previously antagonistic scientificfieldsand enabling South-South coalitions across Latin America. The evidence presented here illustrates how, during times of crisis, states can nationalize fisheriesscience, meaning they promote novel scholarship from domestic data sources, among local scientists, and between endemic research institutions. Thus, the main drivers of epistemic change, in this case, are state actors exercising ?conventional? practices, undermining the assumption that only bottom-up and ?contentious? pressure can do so. This research offers important lessons for future research on state-science relations in the Global South
