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Difference in Moods between Former Athletes and Current Athletes when Exposed to Sports Content
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John Gill and the Covenant of Grace
Full text linkThis study compares John Gill\u27s views on the temporal aspect of the covenant of grace to that of previous Particular Baptists, also known as 1689 Federalism. The central question asked is: Can John Gill be considered a 1689 Federalist? Gill is compared to covenant theologians, both Baptist and paedobaptist, in the 17th century
The Qualification and Standardization of Viral Particle Detection
No full textMicroRNAs are biomarkers for various clinical uses such as diagnosis, prognosis, treatment plans, disease risks, and progression. To better understand the implication and application of microRNAs, bacteriophage RNA was amplified and quantified to obtain quantitative plasmid data rather than on a virion basis. While plasmid isolation may be an extensive process, phage plasmid quantification produces reliable results and, therefore, can be used to create a standard of comparison. By standardizing this process, future phage analysis will have more statistical relevance, less quantification variance due to lysate factors, and the quality of the experiment can be assessed against the universal standard. All of these qualities can later be taken and applied to modern medicine to more efficiently identify, treat, and personalize the medical attention needed for each patient. Experimental techniques include plasmid amplification through the growth and isolation of competent E. coli cells, plasmid purification, and quantitative PCR procedure and analysis
The Quantification and Standardization of Viral Particle Detection
No full textMicroRNAs are biomarkers for various clinical uses such as diagnosis, prognosis, treatment plans, disease risks, and progression. To better understand the implication and application of microRNAs, bacteriophage RNA was amplified and quantified to obtain quantitative plasmid data rather than a virion basis. While plasmid isolation may be an extensive process, phage plasmid quantification produces reliable results and, therefore, can be used to create a standard of comparison. By standardizing this process, future phage analysis will have more statistical relevance, less quantification variance due to lysate factors, and the quality of the experiment can be assessed against the universal standard. Experimental techniques include plasmid amplification through the growth and isolation of competent E. coli cells, plasmid purification, and quantitative PCR procedure and analysis. The standardization results of the experiment were inconclusive due to various factors, including negative control amplification, technical error regarding annealing temperatures, and lack of stability testing
The Development of a New Water-Soluble Zinc Porphyrin, ZnTPP-2MP, as a Photodynamic Therapy Agent
No full textPhotodynamic therapy is a new technique used in cancer treatment involving the use of a photosensitizer and light exposure to kill malignant cells. This research tested the use of ZnTPP-2MP as a photosensitizer using white light as a source of possible treatment of the A549 cancer cell line, with preliminary testing on MYC-22 and other G-quadruplex DNA samples. ZnTPP-2MP was synthesized by the reaction of the porphyrin, ZnTPPC, with 2-amino-2-methyl-1-propanol. Following synthesis, the new porphyrin was purified by column chromatography using Sephadex LH-20 and G-50. To confirm the identity and structure of the product, ZnTPP-2MP was analyzed through nuclear magnetic resonance (NMR), infrared (IR), ultraviolet-visible (UV-vis) and fluorescence spectroscopies. Purity of the final compound was determined using HPLC. A reactive oxygen species (ROS) assay was performed using the novel porphyrin, as well as an MTT assay for cell viability
The Development of a New Water-Soluble Porphyrin, ZnTPP-2MP, as a Photodynamic Therapy Agent
No full textPhotodynamic therapy is a new technique used in cancer treatment involving the use of a photosensitizer and light exposure to kill malignant cells. This research tested the use of ZnTPP-2MP as a photosensitizer using white light as a source of possible treatment of the A549 cancer cell line, with preliminary testing on MYC-22 and other G-quadruplex DNA samples. ZnTPP-2MP was synthesized by the reaction of the porphyrin, ZnTPPC, with 2-amino-2-methyl-1-propanol. Following synthesis, the new porphyrin was purified by column chromatography using Sephadex LH-20 and G-50. To confirm the identity and structure of the product, ZnTPP-2MP was analyzed through nuclear magnetic resonance (NMR), infrared (IR), ultraviolet-visible (UV-vis) and fluorescence spectroscopies. Purity of the final compound was determined using HPLC. A reactive oxygen species (ROS) assay was performed using the novel porphyrin, as well as an MTT assay for cell viability
Preparation and Analysis of Imine-Conjugate Amoxicillin Chitosan Hydrogels for Wound Care Applications
No full textIn the past decade, research has shown the effectiveness of biopolymers for medical applications such as wound dressing, suturing, promoting cell proliferation, and controlled drug administration (Baranwal et al., 2022). Chitosan is a natural biopolymer of high functionality that is comprised of repeating β-(1,4)-2-amino-D-glucose and β-(1,4)-2-acetamido-D-glucose units that are linked by 1,4-β-glycosidic bonds. Chitosan is a highly biocompatible, cost effective, and versatile biopolymer that has merit in several medical applications, including hydrogels (Nicolle et al.,) Due to Chitosan’s large number of functional groups, it is possible to functionalize the polymer with antibiotic and therapeutic compounds through a reversible mechanism. This study explores the effectiveness of chitosan functionalization for drug delivery for wound care. It is possible to functionalize the chitosan at primary alcohol groups with primary amine containing drugs, which includes many antibiotics like amoxicillin, so that they can later be released at the wound site for therapeutic effects. The functionalized chitosan would retain its cross-linking capabilities and allow for comparison of effective drug delivery between an antibiotic functionalized chitosan hydrogel and a traditional antibiotic loaded chitosan hydrogel