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The Effects of Temperature and Phasins on Polyhydroxyalkanoic Acid (PHA) Accumulation in Heterologous Hosts Containing a PHA Synthase from Vibrio species B-18
Full text linkPolyhydroxyalkanoic acids (PHA) are bacterial carbon reserves with application as biodegradable biocompatible plastics. PHA synthase, encoded by phaC, creates the PHA polymer. Evidence presented by many researchers has emphasized the role of phasin proteins in PHA accumulation. Phasins have been implicated in PHA granule localization, PHA synthase activation, and possibly chaperone and nucleoid binding functions. The phaC gene from Vibrio species B-18 with and without an upstream gene showing homology to phasin genes (orf2) were placed into runaway replication vectors, which use temperature to control gene expression. The aim of this thesis is to explore earlier observations that lower temperatures and the presence of orf2 enhance PHA accumulation in enteric bacteria, specifically Escherichia coli and Klebsiella aerogenes. This study also sought to determine the correct transcriptional start site for PhaCB18, which has two potential start sites in close proximity. Cells containing the wildtype genes or one of three phaC mutations were subjected to SDS-PAGE analysis for gene expression and the ability to accumulate PHA via microscopy. The findings support the conclusion that the first ATG within phaC is the transcriptional start for PHA synthase. Furthermore, optimal PHA accumulation occurred only in the presence of orf2. However, this enhancing effect was only observed in cells grown at the lower temperatures that do not promote gene overexpression. Vibrio species B-18 was shown to have a lower growth optimum than either of the enteric hosts and this could explain why PHA accumulation occurred more readily in uninduced cells
Visions in Euphoria
Full text linkVisions in Euphoria captures the lens that Tommy Orange portrays in the duality of the self identity and the images portrayed in the native community. I chose to pick the art of poetry to emphasize each stanza in the symbols of the Great tribes of the land. The symbols that I have grown to respect deeply, as well as my Wampanoag family, all have a uniting factor of power and are the harmonious flows that follow the poem from beginning to end
Free, Flexible and Engaging
Full text linkOER does more than saving students money. This workshop will explore and share ideas for engaging students with OER-Enabled Pedagogy, including: finding OER and openly licensed materials, assignments, engaging students in the licensing process and best practices for sharing student work
The Impact of Rapid Response Team Activation on Unplanned Transfers to the ICU
Full text linkRapid Response Teams (RRTs) were addressed by the Institute for Healthcare Improvement (IHI) as a means for improving inpatient hospital morbidity and mortality. There implementation was encouraged nationwide with the goal to decrease inpatient cardiopulmonary arrests, mortality rates and unplanned admissions to the Intensive Care Unit (ICU). The purpose of this systematic review was to evaluate the impact of RRTs on unplanned transfers to the ICU. A comprehensive literature review was performed using the PubMed database focusing on RRTs and unplanned ICU transfers. The Donabedian model was used as the theory for this review in conjunction with the PRISMA framework. Study specific data and data outcomes were extracted from individual studies and recorded in tables. Critical appraisal of the included studies was performed utilizing the CASP Checklist for cohort studies. Cross study analysis was then performed to compare outcomes of individual studies against one another in the form of a table. The findings of this systematic review addressed the impact of RRT on ICU admissions with varying outcomes in regards to number of patients admitted to the ICU after RRT review, APACHE scores, length of stay, and mortality. Results of this study address limitations of the identified research and recommendations and implications for the role of the advanced practice nurse
Resilience: Semi-Structured Interviews with Oncology Nurses
Full text linkThe purpose of this project is to explore the relationship between resilience and oncology nurses through semi-structured interviews and identify key themes discussed by participants
Reflections on the 2020-2021 School Year
Full text linkhttps://digitalcommons.ric.edu/hbs_time_cap/1001/thumbnail.jp
Cognitive Defusion for Insomnia
Full text linkCognitive defusion interventions were used to in an attempt to help client cope with nighttime anxiety that was interfering with sleep