5646 research outputs found
Sort by
Comparison of S100β Expression in SK-MEL-25 and A-375
No full textTraumatic brain injury (TBI) affects more than 2 million Americans each year. S100β protein acts as a biomarker for TBI– studies have shown that micromolar levels of S100β are dangerous to neuronal health. Our plan for the summer was to experiment with whether miRNA-4705 could inhibit the production of S100β or not. To get started on the transfection, we had two hurdles to overcome. One of them was to find an efficient s100B primary antibody and the second was to ensure our s100B siRNA worked. By analyzing various research papers and their western blot, we identified and selected two s100B primary antibodies. Our study investigated the ability of two S100β siRNA - used to confirm successful transfection of RNA into the selected cell lines - to silence the S100β genes in two cell lines: A-375 and SK-MEL-28. These cells were cultured and plated in 24-well plates. Each plate had 6 conditions: mock (transfection agent RNAiMAX and media), untreated (just media), negative control siRNA (not expected to reduce S100B levels), beta-actin siRNA (A positive control for transfection), S100β-1 siRNA, and S100β-2 siRNA. After 72 hours of incubation, the cells were lysed for the purpose of running a gel electrophoresis and Western blot. Our Western blots showed varying results, though we determined that A-375 cells do not produce enough endogenous S100β protein to be detectable, while SK-MEL-25 does produce S100β protein above levels of detection. Furthermore, we found that S100β siRNA-1 can be used to effectively silence the S100β genes. This will allow us to use S100β siRNA as a positive control for future research with miR-3705. Further research is required to determine whether miR-3705 effectively suppresses the S100β protein. We plan to continue work with the SK-MEL-25 cells and culture a new cell line called C6.https://scholarship.depauw.edu/srfposters/1159/thumbnail.jp
December 2024 Table of Contents Newsletter - Science Librarian / Google Lens
Get PDFAfter many years of animal cams, unwavering support of all the science departments, and the best laugh on campus, the DePauw Libraries are saying merry retirement to Caroline Gilson! Caroline made an indelible mark on students and faculty through her work in science librarianship at DePauw. You can write a thank you to Caroline by stopping by TheDesk at Roy O. West. Join the celebration for Caroline on Thursday, December 5, 1:30-3pm in Roy O. West’s Laptop Lab (119)
Honor and Political Imagination
No full textHonor is often considered to be obsolete--tethered to a distant past of jousting knights and profound inequality. Yet the idea of honor remains a fixture in both political discourse and popular culture, generating an almost mythic source of meaning for those who draw inspiration from it for heroic (and often reckless) acts. Honor can inspire one to act with courage and conviction, but at the same time, it is profoundly destructive and anesthetizes one to the terrible consequences of its pursuit. What accounts for the enduring appeal of honor today?In Honor and Political Imagination, Smita A. Rahman reckons with the power of honor in contemporary political and popular culture and the desire for heroism that accompanies it, while still being attentive to the dangers that such a desire brings. Rahman argues that while there may be a place for honor in the political imagination, it remains a contested and complicated one. Throughout the book, Rahman includes close readings of several visual and literary texts in popular culture--including Game of Thrones, Wolf Hall, and the Marvel Cinematic Universe\u27s Captain America, among others--and puts them in conversation with a range of texts on honor in political theory, including Aristotle, Machiavelli, Hegel, Nietzsche, Appiah, and more. Drawing on the way honor is used in political and popular culture, Honor and Political Imagination shows not only its follies, but also its appeal and ability to inspire heroic political action that embraces life and seeks to overcome resentment
Alpine Jubilance
Get PDFJustine Vandenberg is an Environmental Fellow student at DePauw University majoring in Sustainability Science and Environmental Studies. She is also a writer, poet, and photographer who is passionate about using artistic forms to advocate for the health of ecosystems and human communities. She finds beauty in hiking, gardening, and mountain life
Mass Deconstruction
Get PDFOliver Spencer is a senior writing major from Fort Wayne, Indiana. He loves writing in many genres, but especially creative nonfiction, magical realism, and horror. He is planning on going to graduate school for a degree in library science. Oliver loves birds, theater, and researching
Sunday Morning
Get PDFLeo Hamilton is an Economics major and a Management Fellow at DePauw University. He is from Golden, Colorado and loves the landscapes of the west
BS (a condition)
Get PDFTrista Keeton has always been a writer, ever since she held a pen she’s been writing little stories. The popular kids on the bus would pay her to write a survival plot line that included slight nudity. Now she’s writing at DePauw with no nudity :/ not as fun
Our Blood Feeds The Earth
Get PDFAnne Gregg is a junior English writing and studio art double major from Terre Haute, Indiana. She enjoys using horror and fantasy to create narratives that explore themes of grief and the uncanny
Avoidance behavior of Oppia nitens (oribatid mites) to herbicide: Glyphosate compared to microplastics in standard artificial soil.
Get PDFDespite growing public interest in environmental issues, awareness of contaminants affecting soil organisms remains broadly lacking, despite decades of research. This study investigated the impacts of a common herbicide; glyphosate, (Roundup), on the avoidance behavior of Oppia nitens - a soil mite species used as an indicator of soil health, and soil moisture content. The study involved exposing O.nitens to standard artificial soil treated with glyphosate concentrations ranging from 47.8 to 501.6 mg/kg. Avoidance behavior was assessed by observing mite distribution between treated and untreated soil over a 48-hour period. Results demonstrated clear avoidance behavior at higher glyphosate concentrations (501.6 mg/kg), with EC50 of 4,630 mg/kg suggesting that glyphosate contamination can negatively impact soil ecosystems by affecting key organisms. The testing of water loss between glyphosate and other microplastics such as Polyethylene (PE) and Polystyrene (PS) showed that glyphosate enhances water loss as a desiccant and this is an inverse trend compared with PS and PE. Moreover, when juxtaposing their EC50 with other bioplastics consisting of Poly Vinyl Alcohol (PVA) and Sodium Polyacrylate (NaPa), glyphosate is only less toxic than PS, but significantly more toxic than PE, PVA and NaPa. Loss of soil moisture content can contribute to the overall avoidance behavior in the exposure of Oppia nitens with glyphosate. The observed avoidance of glyphosate-treated soils by O. nitens and possibly other soil fauna such as earthworms or springtails could have significant cascading effects on soil ecosystems. The consequent alteration in mite distribution could potentially impact soil quality and obstruct degradation processes, highlighting the need for further investigation into the long-term impacts of glyphosate on soil biodiversity and health.https://scholarship.depauw.edu/srfposters/1164/thumbnail.jp