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Criopreservação e banco de células: bancos de células
No full textdubladoVídeo integrante do Curso Princípios básicos do cultivo de células de mamíferos, que aborda o subtema Criopreservação e banco de células: Bancos de célulasFiocruzMP4Tópicos em cultura de células de mamíferosPrograma de Pós-Graduação em Biociências e Biotecnologi
COVID-19 in northeast Brazil: first year of the pandemic and uncertainties to come
No full textOBJECTIVE: To analyze the epidemic of COVID-19 in northeastern Brazil, one of the regions
most affected by the virus.
METHODS: The official data for COVID-19, from March 2020 to March 2021 in the states of
the Northeast Region (NE), were used. The analysis of capital cities and states for accumulated
weekly cases and confirmed deaths was made using the JoinPoint Trend Analysis application.
RESULTS: In one year, the Northeast region reported 22.9% of the cases and 21.5% of the deaths
in the country due to COVID-19. At the beginning of the pandemic, all states showed a growing
number of cases, first in the capitals and then in the interior. Following this wave, decreases
are observed in all states and their capitals, but with many still reporting a large number of
cases. In the middle of the 2nd semester of 2020 the number of cases begins to increase again
simultaneously in states and their capitals—some at explosive speed—especially in late 2020
and early 2021. A similar pattern is observed in deaths, which exceed or approach the peak seen
in the first wave. In the first wave, all capitals and northeastern states adopted intense isolation
measures. Fortaleza, Recife and Teresina reached the highest isolation index of all capitals, close
to 0.60. This index decreases, with a slight growth trend until the end of December. With the
exception of Fortaleza and Salvador, the other capitals fell to less than 0.40.
CONCLUSION: The Brazilian NE and the country are in increasingly complicated health,
social and economic situations. It is necessary to speed up vaccinations and maintain nonpharmacological
measures: face masks, social distancing measures and hygiene care, in addition
to policies to protect workers who have lost their incomes and to subsidize small business owners
Informativo nr. 148
No full textBoletim informativo desenvolvido pelo Nethis/Fiocruz Brasília com os principais eventos e notícias. Edição publicada no dia 21/06/2021
Posições da Mulher durante o Trabalho de Parto e Parto: benefícios da livre movimentação
No full textA mulher, que está vivenciando/sentindo em seu próprio corpo, precisa estar livre para mover-se e posicionar-se de forma que encontre conforto e alívio. Este é o caminho para um parto fisiológico e uma experiência positiva.FiocruzText
Gerromorpha (Insecta, Hemiptera, Heteroptera) from eastern Maranhão state, northeastern Brazil
No full textThe infraorder Gerromorpha (Insecta, Hemiptera, Heteroptera) comprises semiaquatic bugs, most of which live on
the water surface, among floating plants or at the margins of water bodies. We made collections of these insects in
the Itapecuru and Parnaíba hydrographical basins in eastern Maranhão state, northeastern Brazil, from January to
December 2019. Based on the material obtained, we present new records for 23 species, 20 of which are recorded for
the first time from Maranhão state
Alginate-chitosan microcapsules improve vaccine potential of gamma-irradiated Listeria monocytogenes against listeriosis in murine model
No full textListeria monocytogenes is a cause of infectious food-borne disease in humans, characterized by neurological manifestations, abortion, and neonatal septicemia. It is intracellular bacterium, which limits the development of protective inactivated vacines. Adjuvants capable of stimulating cellular immune response are important tools for developing novel vaccines against intracellular bacteria. The aim of this study was to evaluate the vaccine potential of L. monocytogenes inactivated by gamma irradiation (KLM-γ) encapsulated in alginate microcapsules associated or not with chitosan against listeriosis in the murine model. At the fourth day after challenge there was a reduction in bacterial recovery in mice vaccinated with KLM-γ encapsulated with alginate or alginate-chitosan, with lower bacterial loads in the spleen (10 fold) and liver (100 fold) when compared to non-vaccinated mice. In vitro stimulation of splenocytes from mice vaccinated with alginate-chitosan-encapsulated KLM-γ resulted in lymphocyte proliferation, increase of proportion of memory CD4+ and CD8+ T cell and production of IL-10 and IFN-γ. Interestingly, the group vaccinated with alginate-chitosan-encapsulated KLM-γ had increased survival to lethal infection with lower L. monocytogenes-induced hepatic inflammation and necrosis. Therefore, KLM-γ encapsulation with alginate-chitosan proved to have potential for development of novel and safe inactivated vaccine formulations against listeriosis
Plasma Metabolomics Reveals Dysregulated Metabolic Signatures in HIV-Associated Immune Reconstitution Inflammatory Syndrome
No full textIntramural Research Program of
the National Institute of Allergy and Infectious Diseases at the
National Institutes of Health (NIH).Immune reconstitution inflammatory syndrome (IRIS) is an inflammatory complication
associated with an underlying opportunistic infection that can be observed in HIV-infected
individuals shortly after the initiation of antiretroviral therapy, despite successful
suppression of HIV viral load and CD4+ T cell recovery. Better understanding of IRIS
pathogenesis would allow for targeted prevention and therapeutic approaches. In this
study, we sought to evaluate the metabolic perturbations in IRIS across longitudinal time
points using an unbiased plasma metabolomics approach as well as integrated analyses
to include plasma inflammatory biomarker profile and whole blood transcriptome. We
found that many lipid and amino acid metabolites differentiated IRIS from non-IRIS
conditions prior to antiretroviral therapy and during the IRIS event, implicating the
association between oxidative stress, tryptophan pathway, and lipid mediated signaling
and the development of IRIS. Lipid and amino acid metabolic pathways also significantly
correlated with inflammatory biomarkers such as IL-12p70 and IL-8 at the IRIS event,
indicating the role of cellular metabolism on cell type specific immune activation during the
IRIS episode and in turn the impact of immune activation on cellular metabolism. In
conclusion, we defined the metabolic profile of IRIS and revealed that perturbations in
metabolism may predispose HIV-infected individuals to IRIS development and contribute to the inflammatory manifestations during the IRIS event. Furthermore, our findings
expanded our current understanding IRIS pathogenesis and highlighted the significance
of lipid and amino acid metabolism in inflammatory complications
Salvar vidas urgentemente
No full textO Brasil cruzou a linha de 250 mil mortos e 10 milhões de casos confirmados pela covid-19 em 24 de
Fevereiro de 2021, com alta na média móvel de mortes que configura segunda onda justamente quando as medidas de
distanciamento social estão sendo afrouxadas. Diante do agravamento da crise sanitária, com colapso do sistema
de saúde em vários estados, a Associação Brasileira de Saúde Coletiva (Abrasco) divulgou nota (27/2/21) intitulada
“Salvar Vidas Urgentemente”, em que alerta para a negligência e a desorganização impostas pelo governo federal
ao enfrentamento do novo coronavírus. “Não é admissível que tantas vidas sejam perdidas”, destaca o texto
Informativo do Observatório Brasileiro de Hábitos Alimentares - nº 7
No full textInformativo elaborado pelo Observatório Brasileiro de Hábitos Alimentares (OBHA) com o propósito de compartilhar reflexões sobre este momento com o propósito de afirmar, construir e reconstruir ações de promoção a alimentação adequada e saudável para durante e após a pandemia do COVID-19