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Portfólio de pesquisas da Rede PMA sobre a Atenção Primária à Saúde: 2020-2023
No full textConcepção: Isabela Soares Santos, Roberta Argento Goldstein, Rosane Marques de Souza, Isabella Koster, Beatriz Soares da Costa. Coordenadores das Pesquisas: Adelyne Maria Mendes Pereira, Alda Maria Lacerda da Costa, Ana Claudia Santiago de Vasconcellos, Ana Patrícia Pereira Morais, Annibal Coelho de Amorim, Anya Pimentel Gomes Fernandes Vieira Meyer, Beatriz Fátima Alves de Oliveira, Camila Pimentel Lopes de Melo, Dalia Elena Romero Montilla, Débora Dupas Gonçalves do Nascimento, Elainne Christine de Souza Gomes, Fernando Ferreira Carneiro, Grácia Maria de Miranda Gondim, Islândia Maria Carvalho de Sousa, Júlio Cesar Schweickardt, Kerla Joeline Lima Monteiro, Laís Silveira Costa, Liane Maria Braga da Silveira, Ligia Giovanella, Luciana Dias de Lima, Márcia Cristina Rodrigues Fausto, Márcia Teixeira, Márcia Valéria Guimarães Cardoso Morosini, Maria das Graças Rojas Soto, Mariana Nogueira, Marly Marques da Cruz, Mauricio Monken, Nathan Aratani, Noemia Urruth Leão Tavares, Paulo Cesar Basta, Paulo Roberto de Abreu Bruno, Raquel Vasconcellos C. de O, Oliveira, Renata de Saldanha da Gama Gracie Carrijo, Renata Palópoli Pícoli Nardoni, Roberta Gondim de Oliveira, Santuzza Arreguy Silva Vitorino, Simone Gonçalves Assis, Sonia Regina da Cunha Barreto Gertner, Tatiana Chama Borges Luz, Vanira Matos Pessoa, Yara Hokerberg
Ética: relato de caso - HeLa
No full textdubladoVídeo integrante do Curso Princípios básicos do cultivo de células de mamíferos, que aborda a subtema Ética: Relato de caso - HeLaFiocruzMP4Tópicos em cultura de células de mamíferosPrograma de Pós-Graduação em Biociências e Biotecnologi
Unveiling six potent and highly selective antileishmanial agents via the open source compound collection ‘Pathogen Box’ Against antimony-sensitive and -resistant Leishmania braziliensis
No full textDespite all efforts to provide new chemical entities to tackle leishmaniases, we are still dependent on a the limited drug arsenal, together with drawbacks like toxicity and drug-resistant parasites. Collaborative drug discovery emerged as an option to speed up the way to find alternative antileishmanial agents. This is the case of Medicines for Malaria Ventures - MMV, that promotes an open source drug discovery initiative to fight diseases worldwide. Here, we screened 400 compounds from ‘Pathogen Box’ (PBox) collection against Leishmania braziliensis, the main etiological agent of cutaneous leishmaniasis in Brazil. Twenty-three compounds were able to inhibit ≥ 80 % L. braziliensis growth at 5 μM. Six out of the PBox selected 23 compounds were found to be highly selective against L. braziliensis intracellular amastigotes with selectivity index varying from > 104 to > 746 and IC50s ranging from 47 to 480 nM. The compounds were also active against antimony-resistant L. braziliensis isolated from the field or laboratory selected mutants, revealing the potential on treating patients infected with drug resistant parasites. Most of the selected compounds were known to be active against kinetoplastids, however, two compounds (MMV688703 and MMV676477) were part of toxoplasmosis and tuberculosis ‘PBox’ disease set, reinforcing the potential of phenotyping screening to unveil drug repurposing. Here we applied a computational prediction of pharmacokinetic properties using the ADMET predictor pkCSM (http://biosig.un imelb.edu.au/pkcsm/). The tool offered clues on potential drug development needs and can support further in vivo studies. Molecular docking analysis identified CRK3 (LbrM.35.0660), CYP450 (LbrM.30.3580) and PKA (LbrM.18.1180) as L. braziliensis targets for MMV676604, MMV688372 and MMV688703, respectively. Compounds from ‘Pathogen Box’ thus represents a new hope for novel (or repurposed) small molecules source to tackle leishmaniase
Optimization of expression and purifcation of Schistosoma mansoni antigens in fusion with Rhizavidin
No full textFAPESP Grant
2017/24632-6 and Fundação Butantan. MMFB received a CAPES fellowship.
VP was registered in the EMJMD LIVE (Erasmus+Mundus
Joint Master Degree Leading International Vaccinology Education), cofunded
by the EACEA (Education, Audiovisual and Culture Executive
Agency, award 2015–2023) of the European commission and received
a scholarship from the EACEA.Schistosomiasis causes signifcant morbidity and mortality. Vaccine eforts to date indicate the need to increase the immunogenicity of Schistosoma antigens. The multiple antigen-presenting system, whereby proteins are genetically fused to rhizavidin and afnity linked to biotinylated templates, enables the generation of robust immune responses. The objective of this work was to express and purify the S. mansoni antigens, SmTSP-2 and SmCD59.2, in fusion with rhizavidin. The fusion with rhizavidin greatly decreased the expression level of rSmTSP-2, but not rSmCD59.2, and both were expressed in the insoluble fraction, requiring optimization of culture conditions. Evaluation of diferent E. coli strains and media showed that BL21-DE3 cultured in Terrifc Broth provided the highest expression levels of both proteins. Investigation of a range of time and temperature of induction showed that E. coli strains expressing rRzv:SmTSP-2 and rRzv:SmCD59.2 showed the highest protein production at 23 °C for 15 h. Recombinant proteins were purifed by a single step of afnity chromatography allowing isolation of these proteins in high concentration and purity. The optimization process increased fnal soluble protein yield of rRzv:SmTSP-2 by fourfold and rRzv:SmCD59.2 by tenfold, providing~20 mg/L of each protein. Optimized fusion protein production will allow antigen use in biotin–rhizavidin afnity platforms
CoronaFatos: Entrevista - HIV e Covid - parte 2
No full textFicha técnica: Apresentação: Ana Cristina Figueira / Produção e Direção: Valéria Mauro / Edição e Finalização: Marcelo Louro / Arte: Marcelo Vianna / Idealização: Canal Saúde - Fundação Oswaldo Cruz.Conforme prometemos, segue a segunda parte da conversa com a médica infectologista, Márcia Rachid, sobre HIV e Covid-19
The relationship of oleic acid/albumin molar ratio and clinical outcomes in leptospirosis
No full textHuman leptospirosis is an acute infectious zoonosis presenting specific lipid disorders. Previous in vitro studies
showed both leptospira glycolipoprotein endotoxin, and high oleic acid levels were associated with Na/K-ATPase
inhibition that is amplified by the reduction of circulating albumin levels. In this study, we aimed to investigate
the relationship of oleic acid/albumin (OA/A) molar ratio and clinical outcomes in Leptospirosis. Through a
prospective observational cohort study employing high-performance liquid chromatography (HPLC) we sequen tially determined serum concentrations of nonesterified fatty acids (NEFA) and albumin in twenty-eight patients
with severe leptospirosis since their hospital admission. Twenty patients recovered, and eight died. Data was
distributed in two groups according to clinical outcomes. Oleic acid/albumin molar ratios (OA/A), initial samples,
were higher than those in healthy donors. The ratio OA/A, however, persisted high in dying patients, whereas
patients who survived had a reduction matching to healthy donors. Biochemical alterations suggest that cure is
correlated to the reestablishment of the OA/A molar ratio, while fatal outcomes related to persisting OA/A im balances. Analysis by receiver operating characteristic (ROC) showed the area under the curve of 0.864 and the
cutoff value of 0.715 being associated with a high odds ratio. Lipid analysis from patients with leptospirosis had
an acute high serum OA/A molar ratio, and sustained imbalance has a high odds ratio and strong correlation with
mortality
Meio de Cultura: componentes dos meios de cultura
No full textdubladoFiocruzMP4Tópicos em cultura de células de mamíferosPrograma de Pós-Graduação em Biociências e Biotecnologi
Human Immunodeficiency Virus Type 2: The Neglected Threat
No full textWest Africa has the highest prevalence of human immunodeficiency virus (HIV)-2 infection
in the world, but a high number of cases has been recognized in Europe, India, and the United States.
The virus is less transmissible than HIV-1, with sexual contacts being the most frequent route of
acquisition. In the absence of specific antiretroviral therapy, most HIV-2 carriers will develop AIDS.
Although, it requires more time than HIV-1 infection, CD4+ T cell decline occurs more slowly in HIV-2
than in HIV-1 patients. HIV-2 is resistant to non-nucleoside reverse transcriptase inhibitors (NNRTIs)
and some protease inhibitors. Misdiagnosis of HIV-2 in patients mistakenly considered HIV-1-
positive or in those with dual infections can cause treatment failures with undetectable HIV-1 RNA.
In this era of global integration, clinicians must be aware of when to consider the diagnosis of HIV-2
infection and how to test for this virus. Although there is debate regarding when therapy should be
initiated and which regimen should be chosen, recent trials have provided important information on
treatment options for HIV-2 infection. In this review, we focus mainly on data available and on the
insight they offer about molecular epidemiology, clinical presentation, antiretroviral therapy, and
diagnostic tests of HIV-2 infection
SARS-CoV-2 S1, S2, M & N antigens expressed in Pichia pastoris: Affordable, safe & effective vaccine for developing countries
No full textOs autores externos submeteram sua publicação para apresentação de trabalho no evento “International Symposium on Immunobiologicals”, que foi coordenado e organizado pelo Instituto de Tecnologia em Imunobiológicos (Bio-Manguinhos), da Fundação Oswaldo Cruz
Relações de trabalho e qualificação profissional dos trabalhadores técnicos em enfermagem que atuam no Centro Cirúrgico: o caso de um hospital federal do município do Rio
No full textEsta pesquisa propõe analisar as implicações da reestruturação produtiva sobre as relações de trabalho e a qualificação para o trabalho dos técnicos de enfermagem que atuam em um centro cirúrgico de um hospital público da cidade do Rio de Janeiro. A aceleração do desenvolvimento tecnológico, sobretudo da microeletrônica e das tecnologias de comunicação e informação, inaugurou o chamado capitalismo flexível que têm como uma de suas características a flexibilização dos vínculos trabalhistas e precarização do trabalho e da vida. As empresas almejavam a otimização do trabalho com consequente redução de custos de produção e aumento da extração de mais valia. Obedecendo a lógica capitalista, a reestruturação produtiva alcança todos os campos produtivos, incluindo a saúde e a formação de seus profissionais de forma a “educa-los” em conformidade as necessidades do capital. O trabalho em saúde se dá nas relações entre profissional e indivíduo ou no coletivo, porém, hoje quase sempre intermediado por tecnologias, transformando as relações de cuidado e do fazer em saúde. A Reforma do Estado que inaugurou o gerencialismo na saúde, acabou por produzir políticas sociais focalizadas, comprometendo o acesso ao atendimento em saúde e transformando as relações de trabalho no setor se tornam cada vez mais precarizadas. Nesse quadro, a possibilidade de concretização das políticas públicas de saúde e os princípios do SUS ficam comprometidos, na medida em que os atendimentos e intervenções sofrem com a diminuição dos investimentos e a precarização do trabalho. Atualmente a necessidade de produzir cada vez mais em saúde com um quantitativo de profissionais atuantes cada vez menor. O trabalho do auxiliar/técnico de enfermagem não é alheio a esse processo. Ele vivencia em sua prática profissional os reflexos de uma crescente privatização da saúde, da flexibilização de seu trabalho e da inserção de novas tecnologias em saúde, como alternativas de tornar o SUS mais “eficiente”.This research proposes to analyze the implications of the productive restructuring on the labor relations and the qualification for the work of the nursing technicians who work in a surgical center of a public hospital in the city of Rio de Janeiro. The acceleration of technological development, especially in microelectronics and communication and information technologies, inaugurated the so-called flexible capitalism which has as one of its characteristics the easing of labor bonds and the precariousness of work and life. Productive restructuring occurs, then, way of redefining work and production chain relationships, based on a growing access to technical and scientific knowledge that took place after the decline of the Fordist model. The companies aimed at optimizing work with a consequent reduction in production costs and increased extraction of added value. Obeying the capitalist logic, the productive restructuring reaches all the productive fields, including the health and training of its professionals in order to “educate” them in accordance with the needs of capital. Health work takes place in the relationships between professional and individual or in the collective, however, today almost always intermediated by technologies, transforming the relationships of care and doing in health. The aconsequence, the political transformations that Brazil has been going through directly affect the health area and have an impact on its workers. The State Reform that inaugurated managerialism in health ended up producing focused social policies, compromising access to health care and transforming working relationships in the health sector that are becoming increasingly precarious. In this context, the possibility of implementing public health policies and the principles of SUS are compromised, as health care and interventions suffer from reduced investments and precarious work. Currently, there is a need to produce more and more in health with an ever smaller number of professionals working. The nursing assistant / technician's work is not alien to this process. He experiences in his professional practice the reflexes of an increasing privatization of health, the flexibility of his work and the insertion of new health technologies, as alternatives to make SUS more "efficient"