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CELLvo Matrix Manufacturing Improvement
Get PDFStemBioSys seeks to streamline their CELLvo matrix manufacturing process in order to decrease the cost of production. The main objectives of this project are to design, build, and test a system that StemBioSys can utilize to complete the decellularization process more quickly, with greater efficiency, and with lower production costs. The following report covers the features of our complete design and its three subsystems that combine to accomplish the individual tasks of the decellularization process. The primary functions of the three subsystems are as follows: the flask organizer holds 6 flasks together throughout the process, the guide positions the flask organizer at the desired angle for aspiration steps, and the liquid network dispenses reagents into the flasks for the washing steps. Following the design overview is an evaluation of the final design through the scope of project requirements and constraints, as well as the associated tests used in assessing the overall system\u27s performance.
The design constraints of sterility, maintaining the integrity of the CELLvo Matrix, remaining within the provided budget, and the available space for the final process were all satisfied through our design solution. The sterility constraint is satisfied with the use of one of the recommended materials in Section 2.6. The integrity of the CELLvo Matrix was confirmed by checking that the matrix was not scratched or damaged during the aspiration or dispensing steps. Our final design was able to complete multiple runs of the decellularization process in a biosafety cabinet (BSC) without any damage to the subsystems or flasks, thus meeting the design constraint for the available space. Our budget was to remain under 223 to satisfy this constraint.
Our final design is held to many project requirements that will be detailed in the following sections. The requirement that our solution is to be optimized for Tl50 cell culture flasks is met through the three subsystems\u27 abilities to specifically fit T150 flasks. The liquid network subsystem is able to satisfy the requirement of effectively washing away all detergent without damaging the matrices, which contributes to the production of a uniform project. Additionally, the liquid network has multiple frames and bases that allow for the device to be versatile and portable. Moreover, the flask organizer and guide subsystems are designed so they can easily be reproduced and sterilized, and the quantity produced can be scaled to accommodate the various sizes of manufacturing runs. Since our final solution did not alter the foundation of the decellularization process, we maintain the assumption that our subsystems are capable of producing a uniform product, can be integrated into the current process, can be easily adopted by staff and are compatible with OSHA codes and prevailing industry standards. In addition, the process must be performed in a biosafety cabinet and should be able to accommodate any other processes conducted simultaneously, which were both satisfied during prototype testing. Lastly, the implementation of our three subsystems decreases the total time required to complete the decellularization process, satisfying this requirement. Our overall project requirement to reduce Full-Time Equivalents (FTEs) was also satisfied, as a reduction in time decreases the necessary time for one person to work.
Overall, the AvENGRs have created a successful, working prototype to streamline the· CELLvo matrix manufacturing process. Since all of our requirements and constraints were met, there are no changes needed to achieve remaining goals, but there is always room for the improvement of our working prototype. As a result of the COVID-19 pandemic, the project remains unfinished in terms of producing several polished copies of each subsystem. Therefore, the AvENGRs have provided manufacturing recommendations should StemBioSys choose to further the development of our solution (Section 2.6 and Appendix 5.4)
Fault Zone Processes and Fluid History in Austin Chalk, Southwest Texas
No full textFaults are commonly the largest fractures in a fracture network, forming the backbone of the permeability system in low-permeability rocks. Faults in outcrops of the Austin Chalk - an important oil and gas reservoir in south Texas, Louisiana, and westernmost Mississippi - exemplify deformation and fluid histories of subseismic-scale faults in mechanically layered chalk-dominated strata. Exposures of near-horizontal Austin Chalk in southwest Texas contain northwest- and southeast-dipping normal faults with exposed heights of meters to tens of meters and displacements of centimeters to decimeters. Local fault dips are steep to vertical through chalk and limestone beds and moderate through marl, mud rock, and clay-rich ash beds, producing refracted fault profiles. Failure surface characteristics indicate various failure modes, including tensile, hybrid, shear, and (locally) compactive shear behavior depending on the host lithology. Dilational zones associated with steep fault segments host calcite veins. Crack-seal textures in fault zone veins record repeated reactivation of refracted fault profiles. Fluid inclusion and stable isotope geochemistry analyses of fault veins indicate formation at depths of 1.4 to 2.9 km (4593 to 9514 ft) and possibly of 4.2 km (13,780 ft). Fluids include locally sourced saline waters and externally sourced waters and oil in rocks that did not reach oil maturation conditions. Inclusions of 30° API to greater than 50° API oil are found in veins 30 km (19 mi) updip from the Eagle Ford Formation oil zone. These results illustrate the migration of oil and other fluids within subseismic-scale Austin Chalk faults and fundamental control of lithology on fault zone architecture
Drama at Dunder Mifflin: Workplace Bullying Discourses on \u3cem\u3eThe Office\u3c/em\u3e
No full textThis study examines the portrayal and affective framing of workplace bullying behaviors on the popular American television show The Office. Quantitative and qualitative content analyses were conducted on 54 episodes spanning the show\u27s nine seasons. Results revealed 331 instances of workplace bullying, for an average of 6.13 bullying behaviors per episode. Workplace bullying behavior on The Office was grouped into five categories: sexual jokes, public humiliation, practical jokes, belittlement, and misuse of authority. In general, instances of workplace bully were scripted as humorous and lacking significant consequences, which could further contribute to social discourses that perpetuate the problem of bullying in real-life workplaces
The Cambridge Guide to Homer
No full textFrom its ancient incarnation as a song to recent translations in modern languages, Homeric epic remains an abiding source of inspiration for both scholars and artists that transcends temporal and linguistic boundaries. The Cambridge Guide to Homer examines the influence and meaning of Homeric poetry from its earliest form as ancient Greek song to its current status in world literature, presenting the information in a synthetic manner that allows the reader to gain an understanding of the different strands of Homeric studies. The volume is structured around three main themes: Homeric Song and Text; the Homeric World, and Homer in the World. Each section starts with a series of \u27macropedia\u27 essays arranged thematically that are accompanied by shorter complementary \u27micropedia\u27 articles. The Cambridge Guide to Homer thus traces the many routes taken by Homeric epic in the ancient world and its continuing relevance in different periods and cultures.https://digitalcommons.trinity.edu/mono/1144/thumbnail.jp
The Glass Transition in a Dense 2D Colloidal Fluid
Get PDFIn this paper a new experimental setup for analyzing the glass phenomenon in two dimensions is presented. Each component of the setup is discussed, as well as some relevant background on glasses, lipid bilayers and vesicles, resolution, and total internal reflection fluorescence microscopy. The experimental protocol is then discussed with specific focus on vesicle formation and filtering, bilayer formation, and glass cleaning. Each prepared sample of small unilamelar vesicles adhered to a supported lipid bilayer was captured for two thousand frames and these frames were analyzed with the Trackpy particle tracking package for Python in order to find the ensemble mean squared displacement of each sample. This was used to determine the viscosity, with a measured viscosity of 4.04 Pa s for a 0.2% biotin surface, and 7.18 Pa s for a 0.5% biotin surface. Finally, possible directions for future work are given
MeXicana Fashions: Politics, Self-Adornment, and Identity Construction
No full textCollecting the perspectives of scholars who reflect on their own relationships to particular garments, analyze the politics of dress, and examine the role of consumerism and entrepreneurialism in the production of creating and selling a style, meXicana Fashions examines and searches for meaning in these visible, performative aspects of identity.
Focusing primarily on Chicanas but also considering trends connected to other Latin American communities, the authors highlight specific constituencies that are defined by region (“Tejana style,” “L.A. style”), age group (“homie,” “chola”), and social class (marked by haute couture labels such as Carolina Herrera and Oscar de la Renta). The essays acknowledge the complex layers of these styles, which are not mutually exclusive but instead reflect a range of intersections in occupation, origin, personality, sexuality, and fads. Other elements include urban indigenous fashion shows, the shifting quinceañera market, “walking altars” on the Days of the Dead, plus-size clothing, huipiles in the workplace, and dressing in drag. Together, these chapters illuminate the full array of messages woven into a vibrant social fabric.https://digitalcommons.trinity.edu/mono/1162/thumbnail.jp
\u27An Unrivaled Brass Lectorium\u27: The Cloisters Lectern and the Gothic Revival in England
No full textThe Cloisters, the Metropolitan Museum of Art’s outpost for medieval art, houses an elaborate brass eagle lectern made in the Netherlands in c. 1500. Purchased in 1968 from Oscott College, it had previously been in St Chad’s Cathedral, Birmingham, a key site of the Gothic Revival in England. This article examines the Cloisters lectern’s sojourn in Victorian Britain, beginning with its arrival on the London art market and publication in Specimens of Ancient Furniture in the 1830s. The main focus is its subsequent association with the architect, designer and theorist A. W. N. Pugin, who designed and furnished St Chad’s as Britain’s first Catholic cathedral since the Reformation. The lectern helped to define the Catholic identity of Pugin’s vision of the revived Gothic style. It also influenced his own designs for metalwork, reflecting his keen interest in Netherlandish brass sculpture
Cloud Sculpture Final Design Report
Get PDFThe design team has improved the functionality of the paper cloud sculpture, created originally by Bridge LLC, to include a colored response to weather or user-based input. In mid March of this year, the scope of our project shifted in order to comply with the distance-learning protocols put in place by the university. Without access to our materials or prototype, additional work shifted to addressing the weaknesses of our most recent full prototype.
Due to the pandemic and lack of access to the labs, the team was not able to implement the final prototype for testing. However, each individual component, namely response to weather conditions in real time, reliable connection to a weather database and fire safety was thoroughly tested and verified. The material used for the cloud is the one suggested by Paper Cloud to make the final product aesthetically pleasing. The facets of the prototype that fall short of our original project plan include ease of maintenance, installation, and ability to transport. While the wire connections in this prototype have little improvement to maintenance ability compared to the original sculpture, we have provided suggestions in the manufacture and structural layout of the weather-adaptive cloud sculpture to address these issues.
Since our team produced a prototype with demonstrable basic functionality, and addressed each weak point of the prototype with specific suggestions, we consider this prototype to be working. For thoroughness and safety, we have included detailed instructions for creating the electronic setup in Appendix A, along with the suggestion of welding wire connections for added stability
Implications for Controls on Upper Cambrian Microbial Build-Ups Across Multiple-Scales, Mason County, Central Texas, USA
No full textThe morphological architecture and distribution of modern and ancient carbonate systems has been shown to follow spatial-self-organization, however, limited studies describe the morphometrics of microbial carbonates. Upper Cambrian microbial-build-ups outcropping in Central Texas, are exposed laterally (plan view), enabling a study of their morphological architecture and spatial distribution. Drone imagery was acquired to capture the outcrop features and develop a digital terrain model (cm scale resolution) for a bedding plane outcrop (600 × 200 m in size). Four scales of microbial growth (S1- few dm, S2- few m, S3- few tens of m, and S4- few hundreds of m) were identified and mapped. A series of morphometric analysis including Ripley\u27s k, univariate, multivariate, and grouping were conducted and results demonstrate that, the scales S1, S2, and S3 display clustering and the spatial organization of microbial-buildups is naturally organized and not random. Further, as the size of the build-ups increases (from S1–S4), the anisotropy (length/width) increases, their shape becomes oblong, and they become aligned (S2–S4) with the inferred regional winds and tide-associated currents (NE-SW according to the present geography). The S1 scale does not align itself with the regional currents; instead, the build-ups behaved as a baffle during growth, and modified the currents locally, leading to preferential alignment at the edges within S2. As the scales increases in sizes (S2, S3, S4), there is competition for space, and due to regional currents, the larger scales preferentially align parallel to high-energy currents. The trends and spatial relationships identified in this study are particularly relevant and provide a scenario for sub-seismic scale heterogeneities for subsurface microbial hydrocarbon reservoirs