Southeast Asian Fisheries Development Center
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SEAFDEC/AQD highlights 2018
SEAFDEC/AQD highlights 2018 is SEAFDEC/AQD's annual report updating on its accomplishments and progress for the year 2018
Fire + water + bombs: Disaster management among academic libraries in Marawi City, Lanao del Sur, Philippines
The academic libraries in Marawi City, Lanao del Sur, Philippines are vulnerable to disasters. In the last ten years, the majority of the 13 respondent libraries have sustained at least one disaster that may have been caused by civil unrest, war or terrorism, flood, earthquake, or fire. The majority were unprepared to face such disasters, may it be small-scale or catastrophic, and only one library has a disaster management plan. The lack of significant holdings of rare books, shortage of financial resources, no perceived risk, and the unavailability of staff to write a disaster management plan, are the reasons why most libraries do not have a plan. Moreover, the majority lacked staff that has undergone training in disaster preparedness and management. On the other hand, all of the libraries have disaster management practices that although not enough, will still enable them to lessen the effects of the disaster and save their library and parts of their collection when necessary. The respondents identified management support as an essential factor in their success in disaster management. The results of the study provide valuable information on the current state of the libraries in the Philippines with regards to disaster preparedness and management. Therefore, it is an essential addition to the literature on disaster management in the Philippines, which is very scarce as of the moment
Draft genome sequence of Vibrio parahaemolyticus strain PH1339, which causes acute hepatopancreatic necrosis disease in shrimp in the Philippines
We report the first draft genome sequence of an acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus strain isolated from a Penaeus vannamei sample from the Philippines. The strain carries the genes encoding the Pir-like toxin pair PirAvp and PirBvp.This research is funded by the Department of Science and Technology of the Philippine Council for Agriculture, Aquatic Natural Resources Research and Development
Draft genome sequence of multidrug-resistant Vibrio parahaemolyticus strain PH698, infecting penaeid shrimp in the Philippines
The emergence of multidrug-resistant bacterial strains in diverse settings has been reported globally. In the Philippine shrimp aquaculture industry, antibiotics are used for the treatment of bacterial diseases during the production cycle. We report the draft genome of Vibrio parahaemolyticus PH698, a multidrug-resistant strain isolated from a Philippine shrimp farm
Aquatic emergency preparedness and response system in Thailand
In Thailand, Department of Fisheries (DoF) is the competent authority for various aspects of aquatic animals including aquatic animal health. There are two principal legislation giving DoF power to apply for aquatic animal disease prevention and control measures in the country as well as import-export control; namely, Royal Ordinance on Fisheries and Animal Epidemic Act.
DoF has two national reference laboratories for aquatic animal health, one is Aquatic Animal Health Research and Development Division (AAHRDD) for freshwater aquatic animal disease diagnosis and another is Songkhla Aquatic Animal Health Research Center (SAAHRC) for brackish water aquatic animal disease diagnosis. Both are ISO/IEC 17025 accredited laboratories. Besides, there are 19 regional laboratories of DoF located in different areas of the country. All of 21 laboratories are responsible for performing disease diagnosis service for fish farmers as well as for disease surveillance. There are a number of surveillance and control programs for aquatic animal diseases in Thailand. Passive surveillance: information can be collected from disease reporting and other sources such as scientific research, news, publications, social network, or rumor. Active surveillance: to provide assurance of disease status for trade purposes, DoF has setup nationally active targeted surveillance program for demonstrating a number of diseases free status of country or farm establishment in accordance with OIE Aquatic Animal Health Code.
There are several farm standards in Thailand such as Good Aquaculture Practices for Hatchery of Disease Free Pacific White Shrimp, Good Aquaculture Practices for Marine Shrimp Farm, and Aquaculture Establishment for Export of Aquatic Animals. Each standard includes necessary biosecurity practices in order to prevent the introduction of pathogenic agent into or spread within or release from the farm. To control domestic movement of live aquatic animals, in normal situation, Aquatic Animal Movement Document should be gained when purchasing as it is beneficial to traceability. However if the disease free zone or disease zone is announced according to Animal Epidemic Act, all transportation of target aquatic animals or carcasses in or out of the defined zone is prohibited, unless a written permission is obtained.
DoF had developed contingency plan for dealing with aquatic animal disease emergencies. In contingency plan, the Provincial Fisheries Officer in responsible area will act as Director of Emergency Aquatic Animal Disease Control Center while Inland Aquaculture Research and Development Center/Coastal Aquaculture Research and Development Center/AAHRDD/SAAHRC will act as disease investigator and laboratory testing. DoF maintains early warning system by supporting staff to attend the meeting and workshop where there is occurrence of new disease, regularly checking local/regional/international disease report database, communicate with competent authority of trade partner, and regularly reporting disease situation to regional and international system. For early detection system, DoF has not only supported expertise and facilities required for laboratories to diagnose different diseases but also conducted training courses for fish farmers, traders and DoF staffs to recognize signs of the listed disease and emerging disease and encouraged them for rapid reporting of the event to the nearest DoF agency for the purpose of immediate investigation. For early response system, when there is serious disease outbreak, aquatic animals in that epidemiological unit should be contained in safety area. Waste water also should not be released from that area without disinfection. Meanwhile, the DoF staffs should investigate the outbreak urgently in order to define disease zone and find out what factors associated with the outbreak so that the disease management or control measure can be applied properly
Components and implementation strategies for effective hazard monitoring and early warning
Effective monitoring of hazardous incidents for timely dissemination of notifications and warnings involves a thoughtful mixture and application of information, technology and intuitional processes. It starts with the identification of the right data - data to be used in decision making processes - from the right sources - authoritative sources that can be trusted and relied upon. Processes must then be developed to routinely and swiftly acquire, process, and ingest these data into an early warning system (EWS). Decision criteria - sometime referred to as business rules - must be established to transform these data into actionable information, including for the dissemination of warning messages. Finally, the warning messages must be quickly and securely transmitted to the intended recipients, often via redundant mechanisms to insure receipt. Of course, warning messages themselves, even if timely, accurate, and actionable, are not sufficient without an overall context in which to assess them as well as pre-established processes for taking actions, sometimes referred to as Standard Operating Procedures (SOPs). However, even the best SOPs will be ineffective if their users are not adequately skilled and knowledgeable. This generally means that a training and exercise program must be a key component of any successful monitoring and warning system. These elements of effective monitoring - and strategies for their implementation - are described and illustrated via the Pacific Disaster Center s DisasterAWARE all-hazards monitoring, early warning and decision support system
Application of poly-β-hydroxybutyrate (PHB)-based biodegradable plastic as artificial substratum in Litopenaeus vannamei culture
The use of artificial substratum made out of poly-β-hydroxybutyrate (PHB) based biodegradable plastic for penaeid shrimp culture was investigated for the first time. Provision of PHB substratum consisting of PHB type P209 (Biomer, Krailling, Germany) to postlarval whiteleg shrimp Litopenaeus vannamei (106 ± 52 mg) resulted in significantly higher survival (P ≤ 0.05) of 83.6 ± 3.4% as compared to 73.9 ± 3.0% for postlarvae provided with conventional substratum consisting of polyvinylchloride (PVC) pipes. Results on final weight showed significantly higher weight (P ≤ 0.05) of 1008.2 ± 4.5 mg in postlarval whiteleg shrimp provided PHB substratum as compared to 893.4 ± 30.0 mg for postlarvae provided with PVC substratum. The trends of higher visit and preference by postlarval whiteleg shrimp on 10-day and 70-day aged PHB substrata over 10-day and 70-day aged PVC substrata suggest the importance of PHB substratum as grazing area rather than as shelter. Test conducted on water quality also showed a trend towards higher total ammonia-nitrogen (TAN) conversion from an initial concentration of 1.75 ± 0.0 mg L−1 to 0.35 mg ± 0.04 mg L−1 after 72 h with PHB substratum while using no substratum and PVC substratum lead to TAN concentrations of 1.28 ± 0.06 mg L−1 and 1.23 ± 0.10 mg L−1, respectively. Overall, this study indicates that artificial substratum consisting of PHB-based biodegradable plastic increases the quality of postlarval whiteleg shrimp and that it contributes to maintaining good water quality
Aquatic emergency preparedness and response systems for effective management of transboundary disease outbreaks in Southeast Asia: Proceedings of ASEAN Regional Technical Consultation, 20-22 August 2018, Centara Grand Central Ladprao, Bangkok, Thailand
Gonad development and size‐at‐maturity of silver therapon Leiopotherapon plumbeus (Kner 1864; Teleostei:Terapontidae) in tropical volcanic lakes in south Luzon, Philippines
Gonad development of the silver therapon Leiopotherapon plumbeus in two volcanic crater lake habitats (Sampaloc Lake, Taal Lake) in south Luzon, Philippines was examined during the annual reproductive cycle. The minimum body size‐at‐maturity of fish in these two lake habitats was also compared. Four gonad development stages were characterized as basis for the classification of ovarian (immature, maturing, mature, spawned) and testicular maturation (immature, maturing, mature) phases. The occurrence of all development stages in individual gonads suggest an asynchronous development whereby advanced stages are recruited continuously from a pool of younger stage germ cells to result in elevated female and male GSI throughout the annual cycle due to active gonadogenesis. Together with the increasing occurrence of advanced stage oocytes and spermatozoa from March until October, the elevated GSI of fish may indicate peak gonadal growth during the onset of the dry season (December–January) for eventual spawning from the beginning (May–June) until the end of the wet season (October–November). In both lake habitats, male fish were smaller than females but, regardless of sex, the minimum size‐at‐maturity of fish in Sampaloc Lake was significantly smaller than fish in Taal Lake. Overall, asynchronous development during oogenesis and spermatogenesis allows for year‐round reproduction of silver therapon, with elevated gonad growth in the dry season in preparation for spawning during the wet season. Compared with fish in Taal Lake, a smaller size‐at‐maturity of fish in Sampaloc Lake may be a response of the wild fishery stock to long‐term high fishing mortality and degradation of the lake habitat
Insights on the seasonal variations of reproductive features in the eastern Atlantic bluefin tuna
The Atlantic Bluefin Tuna (ABFT, Thunnus thynnus) is one of the most intensely exploited fisheries resources in the world. In spite of the years of studies on ABFT, basic aspects of its reproductive biology remain uncertain. To gain insight regarding the seasonal changes of the reproductive characteristics of the eastern stock of ABFT, blood and tissue samples were collected from mature specimens caught in the Mediterranean basin during the reproductive (May-June) and non-reproductive season (Oct-Nov). Histological analysis of the gonads of May-June samples indicated that there were females which were actively spawning (contained post-ovulatory follicles) and females that were not actively spawning that had previtellogenic and fully vitellogenic oocytes. In males, testis were at early or late stage of spermatogenesis during the reproductive season. In Oct-Nov, ovaries contained mostly previtellogenic oocytes as well as β and α atretic follicles while the testis predominantly contained spermatogonia and few cysts with spermatocytes and spermatozoa. Gonadosomatic index (GSI) in females was highest among the actively spawning individuals while in males GSI was higher in early and late spermatogenic individuals compared to those that were spent. Plasma sex steroids levels varied with the reproductive season. In females, estradiol (E2), was higher in May-June while testosterone (T) and progesterone (P) did not vary. In males, E2 and T were higher in May-June while P levels were similar at the two sampling points. Circulating follicle stimulating hormone (FSH) was higher in Oct-Nov than in May-June both in males and females. Vitellogenin (VTG) was detected in plasma from both males and females during the reproductive season with levels in females significantly higher than in males. VTG was undetected in Oct-Nov samples. Since choriogenesis is an important event during follicle growth, the expression of three genes involved in vitelline envelope formation and hardening was measured and results showed significantly higher levels in ovaries in fish caught in May-June with respect to those sampled in Oct-Nov. In addition, a set of genes encoding for ion channels that are responsible for oocyte hydration and buoyancy, as well as sperm viability, were characterized at the two time points, and these were found to be more highly expressed in females during the reproductive season. Finally, the expression level of three mRNAs encoding for different lipid-binding proteins was analyzed with significantly higher levels detected in males, suggesting sex-specific expression. Our findings provide additional information on the reproductive biology of ABFT, particularly on biomarkers for the assessment of the state of maturation of the gonad, highlighting gender-specific signals and seasonal differences