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Transcriptomic and proteomic characterization of extracellular vesicles derived from Merkel cell carcinoma cells
No full textDeciphering the influence of schistosomiasis on the occurrence of dengue virus infections in Madagascar
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Concepts for recognition, evaluation and the classification of chemical structures and substructures in legal texts for processing in databases using selected examples
No full textChemikalien begegnen uns tagtäglich. Unser modernes Leben mit all seinen Annehmlichkeiten ist ohne viele dieser Chemikalien nicht denkbar. Jedoch sind für jeden Stoff und jede Substanz die Nutzungs- und Gefährdungspotenziale ganz unterschiedlich.
Wichtige Bausteine sind in diesem Zusammenhang Gesetze und Normen, die Chemikalien regulieren und ihre Kennzeichnung festlegen. Daher reicht ein Studium allein nicht aus, um alle Chemikalien richtig einschätzen zu können. Um das über Chemikalien vorhandene Wissen angemessen aktuell und verfügbar zu halten, werden sinnvoll Datenbanken eingesetzt.
Wie all dieses Wissen möglichst adäquat in solchen Datenbanken vernetzt abrufbar gemacht werden kann, um es umfassend und korrekt den Nutzern an die Hand zu geben, ist ebenso Thema dieser Arbeit wie die Modellierung der Entscheidungsprozesse zur konkreten Umsetzung an ausgewählten Beispielen.We encounter chemicals every day. Our modern life with all its conveniences would be impossible without many of these chemicals. However, the potential uses and hazards of each substance are very different. Important components in this context are laws that regulate chemicals and specify their labeling. Therefore, a degree in chemistry is not sufficient to be able to correctly assess all chemicals. In order to keep the knowledge available about chemicals adequately up to date and available, databases are reasonably used.
How all this knowledge can be made available as adequately as possible in such databases in a cross-linked way, in order to provide it comprehensively and correctly to the users, is the subject of this work, as well as the modeling for the concrete implementation on selected examples
Development of inhibitors against the ADPR-binding macrodomain of the non-structuralprotein 3 of SARS-CoV-2
No full textAdvancing Peer-To-Peer Fundraising Research: Conceptual and Empirical Insights from Multi-Actor Perspectives
No full textVoraussetzungen für die Assemblierung binärer Proteinstrukturen und das Design anisotroper Protein-Nanokäfige
No full textPrecise assembly of multiple biomacromolecules into well-defined structures and materials is of great importance for various biomedical and nanobiotechnological applications.
Biomaterials will be crucial for a sustainable bio-economy with uses in industry and healthcare alike. In the first part of this work, the assembly requirements for twocomponent materials using supercharged ferritin nanocages as building blocks were investigated. Several variants of ferritin nanocages were designed to determine the surface characteristics necessary for the formation of large-scale binary three-dimensional (3D) assemblies. The newly generated nanocage variants were employed in protein crystallization experiments and macromolecular crystallography analyses. Computational methods were used to complement the experimental findings. By screening of nanocage variant
combinations at various ionic strengths, three essential features for successful assembly could be identified: (1) the presence of a favored crystal contact region, (2) the presence of a charged patch that is not involved in crystal contacts, and (3) sufficiently distinct
surface characteristics between the nanocages. Surprisingly, the absence of non-crystalcontact-mediating patches had a detrimental effect on the assemblies, highlighting their unexpected importance. The formation of unitary, single building block, structures containing either negatively or positively charged nanocages under previously exclusively binary conditions was achieved. These findings will guide future design strategies by offering design principles and demonstrating how supercharging symmetric building blocks
can aid in the assembly of biomacromolecules into extensive binary 3D structures. The second part aimed to extend the palette of available building blocks towards defined cylindrical nanocages. Toward this goal, a design approach focused on hollow dihedral
architectures was conceptualized. These would allow the use of anisotropic nanoparticulate systems such as quantum rods as cargo previously inaccessible for the formation of biohybrid materials. Recently developed design tools were used to generate de novo in silico assemblies. Several existing protein structures were tested for their suitability as building blocks. Finally, assemblies were validated by physics-based simulations and deep-learning-based protein structure prediction tools
