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Subspace-located catalase prevents membrane degradation and dyssynchronization of local calcium release in adult cardiomyocytes
No full textCatalase, a canonical antioxidant enzyme, has been shown to prevent maladaptive oxidative damage and protect against aging-associated deterioration of cellular functions. In cardiac tissue, catalase protects cardiac myocytes against a variety of pathological changes by maintaining normal contractile function. Here we elucidated how catalase can protect cardiomyocyte excitation-contraction (EC) coupling we focused on exploring the subcellular location and specific effects of catalase in cardiomyocytes. To determine the effects of catalase on cardiomyocyte EC-coupling, we used adenoviral overexpression and pharmacological inhibition of catalase with 3AT in cultured adult mouse ventricular myocytes combined with detailed characterization of Ca2+ signaling. We found that endogenous catalase co-localizes with RyRs in the subspace between the junctional sarcoplasmic reticulum (SR) and t-tubules. When virally overexpressed, catalase increases the ROS scavenging capacity of myocytes and enhances Ca2+-release by increasing synchronization of local Ca2+-release. To improve Ca2+-release, catalase acts to prevent membrane peroxidation and prevents degradation of t-tubule structures. Both the functional and structural effects of catalase were reversed by catalase inhibition with 3AT. The effect of catalase on Ca2+-release was more pronounced in cardiomyocytes isolated from transverse aortic constriction-operated, hypertrophied mouse hearts. Overall, our data suggest that catalase located to the subspace has a specific role in stabilizing and protecting Ca2+-release structures from degradation in conditions associated with increased oxidative stress
GRO-seq profiles for nascent transcriptome analysis in mouse microglia (KLA treatment)
No full textThis dataset contains processed GRO-seq data (tagDirectories and UCSC genome browser format bedgraph files created with software Homer) that representaligned counts to the mm9 mouse genome. Murine microglial BV2 cells were maintained with DMEM supplemented with 10% FBS (Hyclone) and penicillin/streptomycin. Cells were collected 1 h after Kdo2 Lipid A (KLA) treatment
Pakotesääntelyn vaikutus oikeushenkilön rangaistusvastuun ja rahanpesulain mukaisten hallinnollisten seuraamusten väliseen suhteeseen
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Dopamiinisäätelyn häiriöön liittyvä lääkityksen liikakäyttö Parkinsonin taudissa
No full textDopamiinisäätelyn häiriö (dopamine dysregulation syndrome, DDS) on harvinainen mutta kliinisesti merkittävä Parkinsonin taudin dopaminergiseen lääkitykseen liittyvä komplikaatio. Se ilmenee riippuvuutena dopamiinijärjestelmään vaikuttaviin lääkkeisiin, erityisesti levodopaan, ja näiden lääkkeiden pakonomaisena käyttönä selvästi motoristen oireiden hoitotarpeen ylittävinä annoksina. DDS on alidiagnosoitu ja vaikeahoitoinen, ja sen varhainen tunnistaminen ja hoidon aloitus on ennusteen kannalta tärkeää. Hoidossa keskeisintä on nopeavaikutteisten dopamiinilääkitysten lopettaminen sekä kokonaisannoksen hallittu pienentäminen potilaan kliinistä tilaa seuraten
Lukiolaisten eritasoisen ilmastovaikuttajuuden tukeminen ilmastokriisiytyneessä maailmassa
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Evaluation of visual ergonomics in microsurgery: a real-time video processing solution
No full textPurpose
Surgeons’ visual and ergonomical challenges are long-standing concerns since the use of microscopes in surgical procedures. Although devices have been improved in the last few decades, the problem of narrow visual fields in high magnification surgeries persists. This study aims to identify the visual ergonomics challenges among microsurgeons and assess the need for novel assistive surgical solutions.
Methods
The study consisted of two parts: a survey and a hands-on experiment. Sixteen surgeons from neurosurgery and otolaryngology (ENT) specialties were invited to semi-structured interviews on challenges encountered when using surgical microscopes and evaluation of the two proposed technological solutions: CPM (context-preserving magnification) and IT (instrument transparency). Following a demonstration of these software solutions, a survey utilizing a Likert scale was administered. The second part of the study involved twelve practicing neurosurgeons who performed a task using a novel solution and compared it to a standard operative microscope setting.
Results
The most common challenges reported were visual obstruction of field by instruments (93.75%), blurring of structures and light reflection (81.25%), and loss of context (68.75%). 50% of surgeons agreed that adjusting zoom and focus takes a considerable amount of time from surgery and 56% stated that they had at least one episode of difficulty seeing depth. Notably, 69% of respondents expressed interest in testing prototypes of both proposed solutions in real surgery, with a particular preference for instrument transparency. Furthermore, the context-preserving magnification solution hands-on trial demonstrated a 40% reduction in task completion time for 60% of participants. However, one participant found no advantage, and others took longer to complete tasks with the solution compared to standard settings.
Conclusion
Our solution addresses the top visual challenges and instrument obstruction remains a top challenge in high magnification microsurgery. We showed that surgeons are highly likely to use novel assistive technologies that provide wider visual field and transparent instruments
