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A gut reaction: effects of an e. coli-produced toxin on gut hormone regulation and gut physiology
No full textEnterotoxigenic Escherichia coli (ETEC) produces the heat-stable enterotoxin b (STb), which is
responsible for secretory diarrhea in humans and animals. STb disrupts intestinal fluid
homeostasis, epithelial barrier function, and promotes apoptosis. Glucagon-like peptide-2 (GLP2) is an intestinotrophic growth hormone secreted by enteroendocrine L-cells which enhances
cellular proliferation, epithelial barrier function, and inhibits apoptosis. As many similarities are
clear between GLP-2 production and secretion pathways and STb cell signaling mechanisms,
this study aims to investigate the effects STb has on the physiology of in-vitro L-cells and the
secretion of GLP-2. Enteroendocrine cells which secreted the highest amounts of growth
hormone were found to be more resistant to STb intoxication. STb was found to enhance the
secretion of GLP-2, potentially due to similar cellular mechanisms of hormone production and
secretion and STb cell signaling. GLP-2 was found to inhibit the toxigenic effects of STb. Future
work will elucidate the mechanism of this interaction. This work will support the use of GLP-2
therapies in enterotoxigenic related diseases.Master of Science (MSc) in Biolog
A system of statistics-based rules for extending surface fracture models
No full textA fracture network model represents interconnected discontinuities within a solid medium.
These can be used for simulating hydraulic flow through fractured rock, which is a critical
concern in selecting nuclear waste repository sites. In the early phases of site selection,
only an incomplete model of surface features is available, often insufficient for a meaningful
hydraulic analysis. In cases such as these, extending the known data into larger plausible
stochastic models can satisfy the needs of hydraulic simulation to provide meaningful insight.
This thesis outlines a procedure that uses analysis of an incomplete dataset of known surface features, and uses its statistical clues as conditioning constraints for producing surface
fracture models that are larger, and more detailed. The models are evaluated in how well
they honour the geostatistics-inspired constraints and characteristics derived from the source
data, and the results are successful in reflecting many of these statistical inputs
Reading ideological silence in late eighteenth-century English fiction: Pierre Macherey's Theory of Literary Production and the novel of Mary Wollstonecraft
No full textEnglish novels by women of the late eighteenth century rarely if ever reflect the improvements
for women’s lives that contemporary radical ideas promised. Using Pierre Macherey’s A Theory
of Literary Production (1966) as a research lens, author and feminist thinker Mary
Wollstonecraft’s political ideas in her famously bold works of non-fiction are read against her
novels Mary, A Fiction (1788) and The Wrongs of Woman, or Maria (1798). Macherey’s critique
is used to trace the gap between philosophical and fictional imaginations of the late eighteenth
century where it becomes clear that ruling modes of ideology are determining the representation
and fate of Wollstonecraft’s heroines. Wollstonecraft’s use of sensibility and the gothic shows us
that there is not language to delineate a successful feminist heroine. The findings of this study
question imagined authorial freedom in the process of literary production and challenge readers
to produce new knowledge through literary criticis
Pour une promotion de la santé mentale au moyen de la spiritualité : un groupe de promotion du bien-être en milieu scolaire
No full textDans le champ du service social, le travailleur social dispose d’un ensemble
d’approches qui lui permettent d’élaborer des hypothèses en vue d’apporter des
solutions aux besoins de la clientèle. Mais, la spiritualité est peu intégrée au sein de
ces diverses approches. Consciente de la faible utilisation de cette approche en
intervention, j’ai profité d’un stage spécialisé de 450 heures qui boucle mes études
de maîtrise en service social pour promouvoir son application en promotion de la
santé mentale auprès des adolescents en milieu scolaire L’intervention et les
résultats auprès d’une cohorte d’adolescents constituent l’objet de cet essai.Maîtrise en service social (M.S.S.
Working models for human capital planning in Northern Ontairo: a model for the primary healthcare workforce - Technical reports
No full textOntario Ministry of Labour, Training and Skills Developmen
The evaluation and application of tourmaline crystal chemistry as an indicator of porphyry-style Cu-Mo-Au mineralization
Get PDFAs the exploration for porphyry Cu-Mo-Au deposits becomes increasingly challenging, there
exists a need for exploration techniques that can detect buried deposits. Tourmaline supergroup
minerals (TSM), a chemically and physically robust phase in the surficial environment, is
investigated for its indicator mineral potential to identify porphyry Cu-Mo-Au systems. Samples
of TSM from mineralized porphyry systems (Cu, ±Au, ±Mo) including Casino (Yukon Territory,
Canada), Coxheath (Nova Scotia, Canada), Donoso Breccia-Los Bronces (Chile), Highland
Valley Copper (British Columbia, Canada), New Afton (British Columbia, Canada), Schaft
Creek (British Columbia, Canada), and Woodjam (British Columbia, Canada) were examined.
Results show that TSM are generally an early hydrothermal mineral predating both
mineralization and alteration assemblages. They form sub- to euhedral crystals that are
exclusively black (in hand sample) and can be found throughout a porphyry system across all
zones of alteration. Major-element chemistry of the TSM is unremarkable, with most having NaMg-Al (dravite) compositions (~80 % of grains) with lesser amounts of schorl (Fe). They exhibit
consistent concentrations of ~2.0 apfu Mg with most plotting along the oxy-dravite–povondraite trend reflecting the dominance of Al3+ ↔ Fe3+ substitution. An investigation of TSM
trace-elements (LA-ICP-MS) revealed key elements (Sr, Pb, Zn, Cu, and Ga) and select element
ratios (Sr/Pb and Zn/Cu) that can be used as discriminators of its environment of formation.
Utilizing an understanding of both intrinsic and extrinsic controls on TSM chemistry is an
effective approach to distinguish TSM forming in porphyry bedrock samples. A decision tree
was developed and applied to TSM grains recovered surrounding two known porphyry deposits
including Casino-YT and Woodjam-BC to assess its effectiveness. Using both textural and
chemical features of TSM from mineralized porphyry systems highlighted above, porphyry- related grains were identified down ice and downstream of both deposits respectively,
highlighting the potential for this method to be used in exploration. The crystal chemistry of
TSM has been demonstrated to be sensitive to its environment of formation allowing for the
discrimination and identification of TSM forming in porphyries from that forming in other
geologic environments and ore systems.Doctor of Philosophy (PhD) in Mineral Deposits and Precambrian Geolog
Pain assessment and management for people with dementia in long-term care in Ontario
Get PDFProviding nursing care for people with dementia and pain has many challenges. The studies
completed for this thesis explore the depths of these challenges by analyzing the concept of
nurses’ clinical judgment in caring for this population, as well as through the analysis of resident
assessments conducted in long-term care facilities in Ontario. By understanding clinical
judgment as well as patterns from resident assessment data, nurses can better understand the
steps required to provide adequate pain management for people with dementia. The findings
show that long term care residents with dementia who have untreated pain exhibit several
behavioral indicators of pain. This supports the creation of policies and best practice guidelines
that include a tailored assessment of pain using specialized knowledge and tools. These studies
strive to improve the quality of life of residents living with dementia and pain by improving pain
management through proper assessment and intervention
The reported use of PE teachers in the Republic of Congo regarding the teaching styles of Mosston and Ashworth.
No full textLe but de cette recherche était d’identifier l’utilisation déclarée des styles d’enseignement de Mosston et Ashworth (2002) et la perception de leur importance par les enseignants d’Éducation Physique et Sportive (EPS) de la République du Congo. Par la suite, les facteurs qui influencent le choix d’utilisation et leur perception des styles d’enseignement avaient été identifiés et évalués. Pour ce faire, un questionnaire américain traduit et validé culturellement a été utilisé pour recueillir les informations. 120 enquêtés exerçant la fonction d’enseignement d’EPS en République du Congo ont été mis à contribution. Les
résultats obtenus démontrent que les styles d’enseignement les plus utilisé sont respectivement le style d’enseignement par commandement, le style d’enseignement par évaluation réciproque, le style d’enseignement autoprogressif et le style d’enseignement auto-évaluatif. Le style d’enseignement
par commandement et le style autoprogressif sont les styles les plus valorisés. Par ailleurs, les différentes caractéristiques telles que le sexe, la catégorie de l’enseignement, le niveau enseigné, l’expérience professionnelle et l’effectif des classes n’influencent pas le choix d’utilisation et la perception évaluative des
styles d’enseignement de Mosston et Ashworth
A study on the Canadian mining industry and the potential for the “Duty to Consult” as a pathway towards reconciliation with Indigenous Peoples: lessons for Brazil
No full textUsing a reconciliation framework, this research examines select Canadian case law and the
evolution of the “duty to consult” to explore the potential lessons for resource extraction in
Brazil. It conducts an analysis of the legal precedents that exist in Canadian common law as they
relate to Indigenous communities and apply the framework drawn to the resource extraction
process with the hope of determining best practices. In Canada, ongoing discussions and
challenges about the importance of land to Indigenous Communities, their culture and traditions
are commonplace, but in Brazil, legal precedents and policies do not appear as well developed.
Indigenous land claims and resource extraction decisions at the countries’ top courts are of
critical importance to both nations given that mining development impacts upon the social,
economic, and cultural aspects of Indigenous Peoples in both countries. This research targets
legal policies, commentary and Court decisions in Canada relating to Indigenous land and the
mining industry, with an emphasis on constitutional law, to compare aspects of land claims and
constitutional rulings and how they have influenced social policy on Indigenous lives in regards
to reconciliation. There are constructive lessons for the Brazilian government emerging from the
comparison regarding how Canadian constitutional law has framed Indigenous rights with regard
to resource development. In this research, I have found in my analysis that the “duty to consult”
it’s an important key element in the path for reconciliation with the Indigenous communities.
This study revealed the influence of colonial social structures that still persevere and directly
affect Indigenous communities in contemporary society
RNA disruption is a widespread phenomenon that is associated with cell death in tumor cells
No full textA recent clinical trial found that chemotherapy-induced tumor rRNA degradation
(RNA disruption) was associated with both pathological complete response (complete tumor
destruction) and extended disease-free survival (DFS) in breast cancer patients treated with
neoadjuvant chemotherapy. The RNA Disruption Assay (RDA) is a useful tool to quantify the
magnitude of RNA disruption occurring within cells or tumors, expressed as the RNA
Disruption Index (RDI), and is currently the subject of an international clinical trial. The
purpose of this study was to investigate the phenomenon of RNA disruption in response to
both chemotherapy agents and cellular stressors in the ovarian carcinoma A2780 cell line and
the breast adenocarcinoma MDA-MB-231 cell line. Also, we assessed the relationship
between RNA disruption and both cell death and RNase L expression using multiple cell
viability assays and CRISPR-Cas9-mediated RNase L knockout clones, respectively.
RNA disruption was induced in response to multiple chemotherapy agents and several
cellular stressors, including oxidative stress, ER stress, protein translation inhibition and
starvation. However, the induction and magnitude of RNA disruption was found to be both
dose- and stressor-specific, and dependent on the cell line studied. Mycoplasma
contaminations of host cell lines were also found to potentiate a markedly different RNA
disruption banding pattern in response to chemotherapy. RNA disruption was also found to be
associated with cell death, as significant increases in RDI values were observed for
chemotherapy doses that induce partial or complete cell death, as measured by cell counting,
and both cellular recovery and flow cytometry DNA content analysis. Finally, knockout of
RNase L expression in the A2780 cell line did not affect RNA disruption, demonstrating that
RNase L in not involved in the RNA disruption mechanism(s). Overall, these findings support
the novel role of RDA as a tool for quantifying tumor cell death in vitro and in vivo