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The effect of nigella sativa on the murine melanoma cell line, B16-BL6
Get PDFNigella sativa, commonly known as black seed, belongs to the botanical family of
Ranunculaceae. Over recent years, there has been a growing interest in natural products
including N. sativa due to their promising as anti-cancer effects. Several in vitro studies
to determine the effect of N. sativa on the growth of the malignant melanoma cell line,
B16-BL6, and non-malignant cell lines were performed. We have shown that treatment of
cells with a 70% ethanol extract of N. sativa can significantly inhibit proliferation of both
malignant and non-malignant cells. Treatment with an aqueous extract of N. sativa can
reduce the growth of malignant cell proliferation while having a lesser effect on non-
malignant cell proliferation. Ethanol extracts of N. sativa can induce apoptosis in treated
B16-BL6 cells as confirmed using the Acridine Orange/Ethidium Bromide staining assay,
Tunel assay, and Caspase activity assay. Some studies revealed that Thymoquinone is an
important bioactive component of N. sativa and has anti-cancer effect. However, we aim
to further investigate the active components of N. sativa using High-Performance Liquid
Chromatography (HPLC) to identify the components that may be more effective in
inhibiting cancer cell proliferation