İstanbul Sağlık ve Teknoloji Üniversitesi Kurumsal Akademik Arşivi
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Eosin-5′-maleimide (EMA)-binding assay as a diagnostic method of hereditary spherocytosis
No full textObjectives Erythrocyte membrane disorders are caused by a deficiency of structural proteins in the erythrocyte membrane. Accurate differential diagnosis within this group of disorders (is essential for appropriate management. The eosin-5′-maleimide (EMA) binding assay is a novel test that is used for the differential diagnosis of erythrocyte membrane disorders. In this study, we have examined and reported blood counts, reticulocyte indices, and the EMA binding assay results with clinical findings of cases admitted to our laboratory for suspected red blood cell (RBC) membrane disorder. Methods We performed the EMA binding assay on the blood samples of 103 patients who were screened for hereditary erythrocyte membrane disorders at the Flow Cytometry Laboratory of Ankara Numune Training and Research Hospital. The total cohort was grouped as patients with hereditary spherocytosis (HS) (n=36) and control group (patients without erythrocyte membrane disorders (n=60), and non-HS patients with a preliminary diagnosis of hemolytic anemia (n=7). The control group included during data collection, the results of the EMA binding assay were recorded along with demographic features, clinical information, morphologic features, blood count parameters, RBC and reticulocyte indices, and a conventional osmotic fragility (OF) test. Receiver Operating Characteristics (ROC) analyses were performed to evaluate the diagnostic accuracy of the EMA binding assay and reticulocyte parameters. Results Both EMA testing and flow cytometric (FC) OF test were significantly lower in overall (n=36), ≤10-year-old (n=12), and >10-year-old (n=24) patients with HS than in healthy controls (p<0.001). The EMA binding assay had 100 % sensitivity and specificity in screening HS. Conclusions Combined with conventional blood tests, clinical findings, and medical history, the EMA binding assay is a reliable and convenient tool for screening for HS and differentiating hereditary erythrocyte membrane disorders
Evaluation of the prevalence and location of second mesiobuccal canals in 2100 upper first and second molar teeth: A cone beam computed tomography study
No full textIt is essential to undertake thorough preparation, debridement and filling of all root canals in order to achieve a clinically successful treatment outcome. A total of 525 CBCT images of both maxillary first and second molars were analyzed. The observations and measurements were positioned 1 millimeter (mm) apically from the pulp base to standardize the methodology for the detection of the second mesiobuccal canal (MB2). To assess the distances between the canals and the relationship between the presence of MB2 and mesiobuccal (MB) root length, MB root length was quantified in millimeters. IBM SPSS Version 21.0 was employed for statistical analyses, with a type I error level of 5% accepted. MB2 was identified in 36.5% of the first maxillary molar (1MM) and second maxillary molar (2MM) teeth of the patients included in the study. When the frequency of MB2 was evaluated according to gender and right-left side, no statistically significant difference was found (p>0.05). When examining the relationship between the presence of MB2 and the length of the MB root, it was observed that only in the left second molars with MB2 present, the root length was slightly shorter. It is our contention that this study will furnish dentists with crucial data that will enhance the efficacy of root canal therapy for these teeth
Enerji metabolizması ve diyet
No full textBeslenme sağlıklı ve dengeli bir yaşam sürdürülebilmesi için en elzem fizyolojik gereksinimlerimizden biridir. Tüketilen gıdalardaki kimyasal bağ enerjisi hücresel solunum olarak da bilinen çeşitli biyokimyasal reaksiyonlar yoluyla vücudun kullanabileceği enerjiye çevrilir. Bu süreç organizmanın değişen koşullara adapte olabilmesi, büyümesi, gelişmesi ve fiziksel aktivitelerinin devamlılığı için oldukça önemlidir (1). Ancak; enerji metabolizması sadece gıdalardaki karbonhidrat, yağ ve proteinlerin oksidasyonu sonucu hücresel enerji üretimi ile sınırlı değildir. Bazal metabolizma hızı, fiziksel aktivite ve besinlerin termik etkisi yoluyla enerji harcanması, serbest enerjinin fazla olması durumunda depolanması ve eksikliğinde ilgili birime transfer edilmesi de bu sürece dahildir
LC-MS/MS methods for the determination of paclitaxel in biological fluids: A review
No full textOncology stands out as a dynamic field of science, driven by contin uous development and innovative research leading to advances in treat ment methods. In this context, paclitaxel is a chemotherapy drug that is effectively used in the treatment of a wide variety of cancer types. The drug’s areas of application include breast, ovarian, bladder, lung, prostate, melanoma, oesophageal cancers, Kaposi’s sarcoma, and various other solid tumours. This review details paclitaxel’s therapeutic indications, its mechanism of action at the cellular level, its role in suppressing tumour cell proliferation, as well as dosage adjustments, infusion protocols, routes of administration, and possible contraindications; it also emphasises the clinical importance of monitoring patients undergoing treatment. Pacli taxel, initially known as “taxol” when it was first discovered, is a natural diterpenoid compound derived from the Pacific yew tree (Taxus brevifo lia). The drug’s discovery was the result of natural product screening pro grammes conducted by the National Cancer Institute (NCI) in the United States in the late 1960s and early 1970s. During this process, research ers examined various plant extracts in an effort to identify compounds with anticancer potential, and paclitaxel’s pronounced cytotoxic effects attracted attention. In particular, its capacity to halt the cell cycle and induce apoptosis by inhibiting microtubule depolymerisation has made paclitaxel a priority agent in anticancer research (1)
Fluorimetric derivatization-based HPLC-FL method for the prototype pharmacokinetic analysis of selexipag in human plasma
No full textA simple and cost-effective HPLC-FL method has been developed for measuring selexipag in human plasma, showcasing its suitability for pharmacokinetic research. Selexipag was precolumn derivatized with 7-chloro-4-nitrobenzofurazan (NBD-Cl) and the fluorescent derivative was separated on a C18 (150 mm × 4.6 mm × 2.6 μm) analytical column at 30 ºC using a mobile phase composed of acetonitrile – 0.1% o-phosphoric acid in water (70:30, v/v) by isocratic elution with flow rate of 1.0 mL min-1. The method was based on measuring the derivative using fluorescence detection (λex = 380 nm, λem = 420 nm). The retention time of selexipag is 6.40 ± 0.01 min. This currently developed method was validated according to EMA criteria by evaluating the specificity, linearity, precision, accuracy, and robustness. The method was determined to be linear in a concentration range of 0.01-20 ng mL-1 with a correlation coefficient of 0.9998. LOD and LOQ were found to be 0.003 and 0.01 ng mL-1, respectively. Intraday and interday RSD values were less than 1.75%. The plasma concentration-time profile and pharmacokinetic parameters such as AUC0–t, AUC0–∞, Cmax, tmax, t1/2, were calculated according to the assays. The presented method can be effectively used for bioequivalence and bioavailability investigations, as well as for routine analysis of the drug in plasma
Farmasötik ve takviye edici yumuşak kapsüllerdeki α-tokoferol ve α-tokoferil asetat’ın yüksek performanslı sıvı kromatografisi ile kantitatif tayini
No full textAim: This study aims to determine the amounts of α-tocopherol (α-toc) and α-tocopheryl acetate (α-tocAc) in soft pharmaceutical capsules and supplements using a chromatographic method. Additionally, method validation parameters for both analytes were evaluated. Method: Analyses were performed using a chromatographic method. As part of the method validation, recovery (% recovery), repeatability (% RSD), linear regression coefficient (R²), limit of detection (LOD), and limit of quantification (LOQ) values were determined. Results: The recovery values were found to be above 95%. Repeatability (%RSD) was calculated as <3%. The linearity (R²) values were obtained as 0.999. For α-toc: LOD: 0.131 µg/mL and 2.228 µg/mL, LOQ: 0.396 µg/mL and 6.752 µg/mL. For α-tocAc: LOD: 0.260 µg/mL, LOQ: 0.787 µg/mL. Conclusion: The chromatographic method used in this study enabled the determination of α-toc and α tocAc with high accuracy and repeatability. This method can be considered a reliable alternative for the analysis of these compounds in pharmaceutical products.Amaç: Bu çalışmada, yumuşak farmasötik kapsüller ve takviyelerde bulunan α-tokoferol (α-tok) ve α tokoferil asetat (α-tokAs) miktarlarının kromatografik yöntem kullanılarak belirlenmesi amaçlanmıştır. Ayrıca, her iki analit için yöntem doğrulama (validasyon) parametreleri değerlendirilmiştir. Yöntem: Analizler kromatografik bir yöntemle gerçekleştirilmiştir. Yöntem validasyonu kapsamında geri kazanım, tekrarlanabilirlik, doğrusal regresyon katsayısı, LOD ve LOQ değerleri belirlenmiştir. Bulgular: Geri kazanım değerleri %95’in üzerinde bulunmuştur. Tekrarlanabilirlik (%RSD) <%3 olarak hesaplanmıştır. Doğrusallık (R²) değerleri 0.999 olarak elde edilmiştir. α-tok için: LOD: 0.131 µg/mL ve 2.228 µg/mL, LOQ: 0.396 µg/mL ve 6.752 µg/mL α-tokAs için: LOD: 0.260 µg/mL, LOQ: 0.787 µg/mL. Sonuç: Çalışmada kullanılan kromatografik yöntem, α-tok ve α-tokAs miktarlarının yüksek doğruluk ve tekrarlanabilirlik ile belirlenmesini sağlamıştır. Yöntem, farmasötik ürünlerdeki bu bileşiklerin analizi için güvenilir bir alternatif olarak değerlendirilebilir
In vitro evaluation of the effect of children's toothpastes with different ingredients on the surface microhardness and surface roughness of artifical caries lesion in primary tooth enamel
No full textBu çalışmanın amacı; son yıllarda florürlü diş macunlarına alternatif olarak üretilmiş hidroksiapatit, propolis, teobromin içeren diş macunlarının ve florürlü diş macununun yapay başlangıç lezyonlarının remineralizasyonunda etkinliğinin; yüzey mikrosertlik analizi ve yüzey pürüzlülüğü analizi ile kantitatif olarak ve taramalı elektron mikroskobu xix (SEM) ile kalitatif olarak in vitro koşullarda karşılaştırmalı olarak değerlendirilmesidir. Süt azı dişlerinden hazırlanan 80 adet mine örneği 40’ı yüzey mikrosertlik, 40’ı yüzey pürüzlülüğü analizi yapılmak üzere rastgele ayrıldı. Mine örnekleri dört çalışma grubu (Zubio® Kids, Glimo® Alfa, B-Good Care®, Oral-B® Junior çocuk diş macunları) ve bir kontrol grubu olan distile su olmak üzere beş gruba ayrıldı (n=8). Başlangıç yüzey mikrosertliği ve yüzey pürüzlülüğü değeri ölçülen örnekler 72 saat, 37°C’de, demineralizasyon solüsyonunda bekletilerek mine yüzeyinde yapay çürük lezyonları oluşturuldu. Demineralizasyon sonrası ölçümleri yapılan mine örnekleri 7 günlük pH siklusu boyunca çalışma gruplarındaki ajanlar ile fırçalandı. pH siklusu sonrası yüzey mikrosertliği ve yüzey pürüzlülüğü ölçümü tekrarlandı. Her gruptan 1 örnek seçilerek başlangıç, demineralizasyon sonrası ve pH siklusu sonrası taramalı elektron mikroskobu (SEM) analizi yapıldı (n=1). İstatistiksel analizde değişkenlerin normal dağılıma uygunluğu histogram grafikleri ve Kolmogorov-Smirnov testi ile incelendi. Ölçülen değerler gruplar arasında karşılaştırılırken ANOVA testi, posthoc analizde ise Tukey testi; grup içinde karşılaştırılırken Bağımlı-Örneklem T Testi kullanıldı. p<0,05 olduğu sonuçlar istatistiksel olarak anlamlı olarak değerlendirildi. Çalışma sonucunda tüm gruplarda fırçalama sonrası yüzey mikrosertlik değerinin demineralizasyon sonrası değerlerinden yüksek ancak başlangıç değerlerinden düşük olduğu belirlenmiştir. Tüm macunlar mine yüzey pürüzlülüğünü azaltırken yalnızca distile su ile fırçalama yüzey pürüzlülüğünü anlamlı olmayan seviyede arttırmıştır. 1450 ppm florürlü diş macunu yüzey pürüzlülüğünü azaltma ve mikrosertlik geri kazanımı sağlamada diğer gruplara göre anlamlı seviyede daha üstün bulunmuştur. Bununla birlikte teobrominli ve propolisli diş macunlarının remineralizasyon etkileri arasında anlamlı bir fark görülmemiştir, hidroksiapatitli diş macunu diğer diş macunu gruplarına göre anlamlı seviyede daha az yüzey mikrosertlik geri kazanımı sağlamıştır. SEM analizi ile elde edilen görüntüler mikrosertlik ve yüzey pürüzlülüğü bulgularını desteklemiştir. Bu çalışmanın bulgularına göre en yüksek remineralizasyon potansiyelini Oral-B® Junior çocuk diş macunu göstermiştir. Sonuç olarak, çocuklarda diş çürüğünü önlemede en iyi seçeneğin halen florürlü çocuk diş macunu olduğu; hidroksiapatit, propolis ve teobrominli çocuk diş macunlarının ise florürlü diş macunlarına alternatif olabileceği düşünülmektedir.The aim of this study is to evaluate the effectiveness of hydroxyapatite, propolis, theobromine containing toothpastes and fluoride toothpaste, which have been produced as alternatives to fluoride toothpastes in recent years, in the remineralization of artificial xxi incipient lesions, quantitatively by surface microhardness analysis and surface roughness analysis, and qualitatively by scanning electron microscopy (SEM) under in vitro conditions. Eighty enamel samples prepared from primary molars were randomly divided into 40 for surface microhardness and 40 for surface roughness analysis. Enamel samples were divided into five groups (n=8) as four study groups (Zubio® Kids, Glimo® Alfa, B Good Care®, Oral-B® Junior toothpastes) and a control group of distilled water. The samples with initial surface microhardness and surface roughness values were kept in demineralization solution for 72 hours at 37°C to create artificial caries lesions on the enamel surface. Enamel samples measured after demineralization were brushed with the agents in the study groups during a 7day pH cycle. Surface microhardness and surface roughness measurements were repeated after the pH cycle. Scanning electron microscope (SEM) analysis was performed at the beginning, after demineralization and after pH cycling by selecting 1 sample from each group (n=1). In statistical analysis, the conformity of variables to normal distribution was examined with histogram graphics and Kolmogorov-Smirnov test. ANOVA test was used when comparing measured values between groups, Tukey test was used in posthoc analysis; Dependent-Sample T Test was used when comparing within groups. The results were evaluated as statistically significant when p<0.05. In all groups, surface microhardness values after brushing were higher than the values after demineralization and lower than the initial values. All pastes reduced enamel surface roughness, only brushing with distilled water increased surface roughness at a non-significant level. After pH cycle, 1450 ppm fluoride toothpaste was the group with the least surface roughness and the highest microhardness value. No significant difference was observed between the remineralization potentials of theobromine and propolis toothpastes. The microhardness value of hydroxyapatite toothpaste was the lowest compared to the other toothpaste groups. The images obtained with SEM analysis supported the findings of microhardness and surface roughness analysis. According to the findings of this study, Oral-B Junior® 1450 ppm fluoride toothpaste showed the highest remineralization potential. As a result, fluoride toothpaste remains the most effective option for preventing dental caries in children, while toothpastes containing hydroxyapatite, propolis, and theobromine may be considered as potential alternatives
Anatomy based computer assisted evaluation for aesthetic new navel formation
No full textBackground This study aimed to determine the typical position and shape of the umbilicus among young individuals to guide the repositioning of the new umbilicus during abdominoplasty. Methods Standardized photographs of 63 young volunteers were analyzed to evaluate parameters such as the vertical and horizontal positions, length, ratio, and shape of the umbilicus. The study also established the mathematical relationships between the umbilicus and nearby anatomical structures like the xiphoid process, pubic symphysis, anterior superior iliac spine, iliac crests, and body mass index (BMI) to identify the ideal geometric shapes and positions. In two patients with abdominal skin sagging, abdominoplasty was performed with a new umbilicus designed according to these anatomical measurements. Results The umbilici were categorized by shape: vertical oval with superior hooding (29%), round (27%), vertical lozenge (21%), and protruded (11%). Ratios between anatomical points were measured, such as the ratio of the distance between the xiphoid process and the upper edge of the umbilicus to the distance between the lower edge of the umbilicus and the sym physis pubis, which was 1.64±0.40. Post-operative evaluation emphasized achieving a xiphoid-umbilicus/pubic symphysis ratio of 1.6:1 for the new umbilicus, with an aesthetically ideal ratio considered to be 1:1. A strong correlation was found between BMI and all umbilical ratios. Conclusions The most aesthetically pleasing navel position aligns with the golden ratio between xiphoid and umbilicus compared to the umbilicus and abdominal crease. Weight control is also crucial in achieving optimal belly aesthetics
Organisational commitment among nurses: A qualitative study
No full textObjective: To describe the perceptions and experiences related to the organisational commitment of nurses working in healthcare institutions across different regions of Brazil. Design: Descriptive qualitative study. Setting: Registered nurses working in Brazilian healthcare institutions. Methods: An online data collection tool was used. A total of 55 registered nurses participated in the study. Data were collected from July 15, 2023, to September 20, 2023. A thematic analysis was conducted. Results: Data were organised into four themes: reasons to stay in the organisation, feelings towards the organisation, factors that promote organisational commitment, and factors that hinder organisational commitment. A total of 14 subthemes were identified. Conclusions: The study sheds light on the indispensable significance of leadership styles and strategic formulations in improving working conditions, thus driving organisational commitment among nurses. Healthcare institutions could implement pilot projects to strengthen the leadership skills of nurse managers, systematically assessing their influence on workplace satisfaction and commitment
DeepMatch: A BERT-powered talent matchmaking approach
No full textConsultancy companies aim to match their employees to customer projects based on their employee’s talents. Traditional matchmaking methodologies are founded on manual processes that rely on rules of thumb or algorithms that are based on handcrafted heuristics, which cause the matchings to be not only sub-optimal, but also time-consuming, subjective, and prone to human errors. In this paper, we propose a novel consultancy matching algorithm that utilizes BERT to semantically find the most optimal consultant-project matchings for a given set of consultants and projects, pairing relevant project specifications with consultant specifications using the JVSAP algorithm. In doing so, our proposed talent matchmaking system may be utilized to improve the accuracy and efficiency of consultancy matching, thereby facilitating more effective consultancy engagements. Our findings suggest that the pairings demonstrate a discernible alignment with human intuition, as evidenced by the consistent correlation between consultants possessing domain-specific expertise and projects characterized by corresponding thematic descriptions. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2025