The Graduate University for Advanced Studies, SOKENDAI
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Gradual Domain Adaptation with Multifidelity Learning and Generative Model
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Probing the Magnetic Fields of Distant Galaxies to Unravel the Evolution of Galactic Magnetic Fields
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Study on the transition of anisotropy in polygonal crack patterns of paste and its application to Martian terrain
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Identification of physiological substrates for depalmitoylating enzyme ABHD17 in neurons
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Room-Temperature Solution Fluorescence Excitation Correlation Spectroscopy
Get PDFapplication/pdfSingle-molecule fluorescence spectroscopy is a powerful tool to investigate the physical properties of individual molecules. Yet, elucidating the fast fluctuation dynamics of freely diffusing single molecules in solution at room temperature, where a variety of chemical and biological processes occur, remains challenging. In this study, we report on fluorescence excitation correlation spectroscopy (FECS) of room-temperature solutions, which enables the study of spontaneous fluctuation of the excitation spectrum with microsecond time resolution. By employing Fourier transform spectroscopy with broadband femtosecond pulses and time-correlated single-photon counting, we achieved fluorescence excitation spectroscopy of a roomtemperature
solution at the single-molecule level. Building upon this single-molecule measurement, we obtained an excitation wavelength-resolved fluorescence autocorrelation function in the microsecond to millisecond range, demonstrating the potential of this method to elucidate fast, spontaneous, timedependent changes of excitation spectra in statistically equilibrium systems. With further development, this method will allow the study of spectral exchange associated with transitions between sub-ensembles of solution-phase molecules with unprecedented time resolution.journal articl
Characterization of planarian adult pluripotent stem cells by single-cell transcriptome analysis
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タイ東北部における土器づくりと職人をめぐる人類学的研究 ――土器生産地ダーン・クウィアンの形成
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Quantum Beam Analysis for Development of Polymer Electrolyte Fuel Cells
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Functional analysis of ABCB14 in Physcomitrium patens reveals a regulatory mechanism for anisotropic cell expansion through cuticle deposition
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Spatial and temporal variations in density, microstructure, and chemical properties of near-surface firn in Dronning Maud Land, East Antarctica
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