Naresuan University Journal
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    Development of Flood Drain Direction using Mobile GIS and Spatial Interpolation Techniques in Municipality of Khon Kaen, Thailand

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           In this paper, techniques for interpolation of spatial elevation from elevation data by using mobile GIS field survey for flood drain direction mapping have been developed which can be served as a guideline for remote sensing and GIS operations to improve the efficiency of flood disaster monitoring and management in the municipality of Khon Kaen, Thailand. In order to estimate the interpolation of spatial elevation for flood direction, five GIS data were used such as satellite imagery, hydrology, road, boundary and elevation. The paper presents the flood drain direction mapping by using DEM data from IDW interpolation technique. The result was shown the flood water direction from Nong Khot lake and flow to Bueng Kaen Nakhon lake and the last flow to Bueng Thung Sang lake, respectively. Keywords: Mobile GIS, Inverse distance weight, Ordinary Kriging, Flood drain direction, Municipality of Khon Kae

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    Identification of Five Sibling Species of the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand by a Polymerase Chain Reaction Assay

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    Anopheles barbirostris is a complex of six sibling species that are almost identical in adult morphology.  The lack of accurate identification method, diagnosis based on only adult morphological characters has led to misidentification.  This complex has been incriminated, as malaria and filarial vector in Indonesia and Thailand but knowledge of the epidemiology and vectorial roles of this species group are poorly known.  For this purpose, a diagnostic PCR system to differentiate among five sibling species of the An. barbirostris species complex in Thailand was developed by using two PCR reactions.  Based on nucleotide differences in the ITS2 sequences, novel degenerate primer was constructed for amplification in combination with a universal reverse primer to generate amplification products of specific length for each of species.  The first PCR reaction can identify An. barbirostris species complex in four species, i.e., An. dissidens, An. saeungae, An. barbirostris species A3, and An. barbirostris s.s. by using universal primer pairs and the second PCR reaction can distinguish species An. barbirostris s.s. from An. wejchoochotei by using new primer and universal reverse primer.  Additionally, An. saeungae can be clearly differentiated on PCR product from An. barbirostris s.s. and An. wejchoochotei by using new primer

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