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Torus Knot (2,3) Sycamore wood, Figure 3
No full textA (2,3) torus knots, trefoil knot carved on a torus, from a 2 x4 x4 block of Sycamore wood. It is 3.5 in diameter, 1.75 deep.https://orb.binghamton.edu/mathematical_sculptures/1650/thumbnail.jp
Ceramic Studio Practice: Stoneware Bisque Bowls for Alberto Zalma Art, Santa Fe, NM, 2022 (iii)
No full textStoneware produced for Alberto Zalma Art while in residence at Paseo Pottery, Santa Fe, NM, 2022
All rights reserved, Christopher Southward @ Southward Ceramics LLC (2022
Land Drill
Get PDFDylan Brown is a writer and actor based in Brooklyn, NY. She received her BFA in Drama from NYU’s Tisch School of the Arts and her MFA in Creative Writing from Drexel University. Her short fiction has been published by Free Spirit and by Short Édition. For more information, visit www.dylanbrown.ne
Fake Nails and a Gun
Get PDFAngela Miyuki Mackintosh is a writer and illustrator living in the Sequoia National Forest. Her writing has been published in Writer’s Digest, Under the Sun, The Nervous Breakdown, Eastern Iowa Review, X-R-A-Y Literary Magazine, Exposition Review, and Permafrost, among others. Her essay, “Super Bloom” was nominated for a 2023 Pushcart Prize. She’s the founding editor of WOW! Women on Writing. When she’s not writing or editing, she enjoys oil painting, trail running, and cat rescue
Self-Introductions
Get PDFAnika Somaia is an Indian-American student and writer. She is currently co-director at EmpowerHer*Voice, where she runs the annual creative writing contest. In her free time, she enjoys working on public art projects in urban spaces
the universe next door
Get PDFSera Gamble’s writing has appeared in The Washington Square Review, Tinderbox, Nine Mile, Sky Island Journal, thimble, The Wall Street Journal, Nerve, and in various anthologies. She also writes film and television. Sera is a first-generation American living in Los Angeles with her husband and dog. She’s spent a lot of time lately wondering if we’re living in a simulation
Pool Toys
Get PDFAndie Klarin is a queer Jewish poet. Their most recent work has been published in Chaotic Merge: The Identity Zine and Revolute vol.3. You can find them on Twitter @Andie_Klarin, @andiekkarin on Instagram, or in real life somewhere in the woods
“Take an Ounce of Suffolk Cheese”: Home Repair of Eighteenth Century Ceramics at Ferry Farm, George Washington’s Boyhood Home
Get PDFThe archaeological discovery at Ferry Farm of eighteenth century glue residues on tea and tablewares belonging to George Washington’s mother, Mary, raised a number of questions. Although recent research in the archaeological and decorative arts community on repaired ceramic and glasswares was helpful to some extent it primarily focused on professional repairs. At-home mending remained a mystery. Archaeologists at Ferry Farm responded by conducting extensive experimental archaeology on historic glues, replicating period glue recipes to determine the properties of these historic adhesives. Additionally, residue samples of suspected glue were analyzed by chemists from Eastern Michigan and Lourdes Universities utilizing Direct Analysis in Real Time (DART) Mass Spectrometry. The resulting data have shed light on what these sociotechnic artifacts say about a woman in Mary Washington’s social and economic position while highlighting an extremely common yet archaeologically ephemeral activity
The synthesis of 3-formyltyrosine for the purpose of in virto fluorescent visualization of microtubules
Get PDFMicrotubules and the desire to visualize them has been of utmost importance in understanding the behavior of the cytoskeleton. Even further, scientists are using this knowledge to elucidate cancerous activities as they occur within cells. In order to acquire this knowledge, the following methods have been established: the expression of tubulin after it has been fused with a fluorescent protein, the insertion of tubulin that has been exogenously labeled, and the use of fluorescent-probe conjugated microtubule ligands, such as paclitaxel. These methods, however, tend to distort the protein’s native function, thereby preventing an accurate analysis. This concern drives the need for an innovative approach that does not involve bulky attachments or genetic manipulation. One proposed approach is through the exploitation of specific, innate cellular processes, such as the post-translational modification responsible for the tyrosination of α-tubulin. By labeling α-tubulin with a tyrosine derivative capable of reacting with a fluorescent probe, the cellular activities pertaining to cancer can effectively be monitored.
In order to test this approach, 3-formyltyrosine (3FY) was synthesized and ligated in vitro to the α-tubulin subunit of microtubules in prostate cancer cells (PC3s) via the post-translational tyrosination facilitated by tubulin tyrosine ligase (TTL). A functionalized fluorophore, 3-trifluoromethyl-7-hydrazinyl coumarin (TFCH), was chosen as a means to optimize this technique which had previously been done with coumarin hydrazine. Coumarin hydrazine, although effective in labeling, mostly absorbs in the UV range and photo-decomposes easily. TFCH proves a better fluorophore for this process since it both absorbs at 405 nm and exhibits greater photostability.
Although there was no definitive conclusion regarding TFCH’s effectiveness in 3 microtubule visualization, the conditions and experimental procedure were further elucidated in such a way that should hopefully eliminate possible pitfalls in the future. Thus, this study serves as a foundation for the following studies to explore TFCH, as well as other fluorophores, both in vitro and in living cells