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    Wiedza pielęgniarek na temat sepsy: opracowanie i testowanie psychometryczne nowego narzędzia

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    NURSES’ KNOWLEDGE ABOUT SEPSIS: DEVELOPMENT AND PSYCHOMETRIC TESTING OF A NEW INSTRUMENT Aim. The aim of the study was to develop and psychometrically test nurses’ knowledge about Sepsis Scale. Material and methods. A 7-step approach, including item generation, content adequacy assessment, questionnaire administration, factor analysis, internal consistency assessment, construct validity, and replication was used to identify and validate the scale. Validation was carried out among a convenience sample of 265 participants over a period of three years. Results. Exploratory factor analysis showed that a three-factor structure best fit the data. The reliability of the two factors based on the Kuder–Richardson Formula 20 was for the knowledge factor 0.702, for the proceedings factor was a score of 0.631. For the attitude factor Cronbach’s Alpha was 0.884.  Conclusions. Having an appropriate tool to test knowledge levels is crucial for determining the number of cases and deaths due to sepsis; thus, creating a tool consisting of knowledge research is the best way to assess nurses’ general knowledge about sepsis. The instrument will be useful for nurses and healthcare managers for assessing their knowledge of sepsis, monitoring changes in knowledge throughout professional development, and evaluating both the need for and effectiveness  of training programs.WIEDZA PIELĘGNIAREK NA TEMAT SEPSY: OPRACOWANIE I TESTOWANIE PSYCHOMETRYCZNE NOWEGO NARZĘDZIA Cel pracy. Celem badania było opracowanie oraz psychometryczna ocena skali mierzącej poziom wiedzy pielęgniarek na temat sepsy.  Materiał i metody. W celu opracowania i walidacji skali zastosowano siedmioetapowe podejście obejmujące: generowanie pozycji, ocenę adekwatności treści, administrację kwestionariusza, analizę czynnikową, ocenę spójności wewnętrznej, ocenę trafności konstruktu oraz replikację. Walidacja została przeprowadzona na dogodnie dobranej próbie 265 uczestników w okresie trzech lat. Wyniki. Eksploracyjna analiza czynnikowa wykazała, że najlepszym dopasowaniem do danych była struktura trójczynnikowa. Rzetelność poszczególnych czynników, oceniona za pomocą wzoru Kuder-Richardson 20, wyniosła 0,702 dla czynnika wiedzy oraz 0,631 dla czynnika dotyczącego postępowania. Dla czynnika postawy współczynnik alfa Cronbacha wyniósł 0,884. Wnioski. Dysponowanie odpowiednim narzędziem do oceny poziomu wiedzy jest kluczowe dla określenia liczby przypadków sepsy oraz związanej z nią śmiertelności. Opracowanie narzędzia badającego poziom wiedzy pielęgniarek na temat sepsy pozwala na rzetelną ocenę ich kompetencji w tym zakresie. Prezentowana skala będzie przydatna zarówno dla pielęgniarek, jak i menedżerów ochrony zdrowia w ocenie poziomu wiedzy o sepsie, monitorowaniu jej zmian w trakcie rozwoju zawodowego oraz weryfikacji potrzeby i skuteczności szkoleń

    Perspektywy studentów pielęgniarstwa dotyczące skuteczności i zastosowania coachingu zdrowotnego

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    NURSING STUDENTS’ PERSPECTIVES ON THE EFFECTIVENESS AND APPLICABILITY OF HEALTH COACHING Introduction. Health coaching is an important approach to empowering patients for better self-care. The aim of the study was to investigate  the experiences of nursing students with providing health coaching in terms of its effectiveness and usefulness. Methods. Data were collected from 47 reflection forms completed by 3rd-year nursing students after health coaching practice. Results. Students observed improvements in coachees’ health, behaviour, emotional and psychosocial wellbeing, and organization of daily life. Health coaching was seen as useful, broadly applicable approach with a positive impact on coachees’ proactive role. Discussion. Students considered health coaching as an effective approach to improve quality of care and to reduce healthcare burden. They also recognized the clinical effectiveness of the approach. The findings are consistent with other studies on the effectiveness of health coaching. Conclusions. Integrating of health coaching into nursing curricula have potential to enhance professional development and improve the quality of nursing practice. Further research is needed to evaluate  effectiveness of integration health coaching into nursing education.PERSPEKTYWY STUDENTÓW PIELĘGNIARSTWA DOTYCZĄCE SKUTECZNOŚCI I ZASTOSOWANIA COACHINGU ZDROWOTNEGO Wprowadzenie. Coaching zdrowotny jest ważnym podejściem do wzmacniania pozycji pacjentów w celu lepszej samoopieki. Celem badania było zbadanie doświadczeń studentów pielęgniarstwa w prowadzeniu coachingu zdrowotnego pod kątem jego skuteczności i przydatności. Metody. Dane zebrano z 47 formularzy refleksji wypełnionych przez studentów trzeciego roku pielęgniarstwa po praktyce coachingu zdrowotnego. Wyniki. Studenci zaobserwowali poprawę stanu zdrowia, zachowania, dobrostanu emocjonalnego i psychospołecznego oraz organizacji życia codziennego osób korzystających z coachingu. Coaching zdrowotny był postrzegany jako użyteczne podejście o szerokim zastosowaniu, mające pozytywny wpływ na proaktywną rolę coachees. Dyskusja. Studenci uznali coaching zdrowotny za skuteczne podejście do poprawy jakości opieki i zmniejszenia obciążeń związanych z opieką zdrowotną. Uznali również skuteczność kliniczną tego podejścia. Wyniki są spójne z innymi badaniami dotyczącymi skuteczności coachingu zdrowotnego. Wnioski. Włączenie coachingu zdrowotnego do programów nauczania pielęgniarstwa może potencjalnie zwiększyć rozwój zawodowy i poprawić jakość praktyki pielęgniarskiej. Potrzebne są dalsze badania w celu oceny skuteczności integracji coachingu zdrowotnego z edukacją pielęgniarską

    In vitro propagated Mentha rotundifolia (L.) Huds and antioxidant activity of its essential oil

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    Mentha rotundifolia (L.) Huds is an aromatic plant used for its medicinal values. This study aims to select appropriate conditions forin vitropropagation ofM. rotundifolia(L.) Huds and to evaluate yield and antioxidants activity of its essential oils (EOs).The explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators 6-benzylaminopurine (BAP) and Gibberellin (GA3). Hydrodistillated EOs obtained from acclimatized and mother plant, were evaluated for their antioxidant activity. Tests were performed on DPPH free radical-scavenging, ABTS and CUPRAC assays.Shoot induction and multiplication were successfully carried out on MS medium supplemented with the following hormones combinations: 1 mg/l BAP, 1 mg/l GA3and 0.5 mg/l BAP, 0.5 mg/l GA3, respectively. Stem length, nodes and leaves number measured from developmentvitroplantwere 6.89 cm, 5.22 nodes and 11.92 leaves pervitroplant, respectively.In vitro rooted plants were successfully acclimatized at a temperature of 23 ± 2°C and a long day photoperiod with a total survival rate exceeding 95%.EO yield of acclimated plant varied between (0.88-1.49 ml/100 g dry matter) compared to wild plant (0.73 ml/100 g dry matter). The antioxidant potential of EOs from acclimated plant showed on DPPH free radical-scavenging, ABTS and CUPRAC assays values of (IC50: 4.18-24.93 mg/ml), (IC50: 0.51-1.56 mg/ml) and (A0.50: 0.34-2.71 mg/ml), respectively. In contrast, the wild plant exhibited on the same tests the values of (IC50: 10.35 mg/ml), (IC50: 0.12 mg/ml) and (A0.50: 0.99 mg/ml), respectively.The results suggest that micropropagation of M. rotundifolia (L.) Huds can be an interesting alternative for producing important plant material with the possibility to modulate EO yield and its antioxidant potential for future commercial purpose

    Pathological lesions in the oral cavity in the course of connective tissue diseases

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    Dentistry, is one of the intensively and rapidly growing branches of medicine. This prompts dentists to take an interdisciplinary approach to their patients. Thus, the dentist, being a general practitioner, can make significant contributions to the early diagnosis of systemic disease and the faster implementation of appropriate treatment. In view of the aforementioned, we undertook research on the relationship of pathological changes observed in the oral cavity with diseases of the connective tissue system. Collagenosis is a chronic autoimmune disease initiated by many factors, among which the genetic factor and viral infections are mentioned. The changes observed in the oral cavity may be a picture of the disease, a complication of the disease or a side effect of the treatment. The aim of the study is, thus, too present the pathological changes in the oral cavity which often accompany collagenosis, and to discuss the risk factors of connective tissue system diseases and methods of dental treatment

    Comparison of two molecular docking programs: the accuracy of ligand pose prediction

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    The study was perform to compare the output of two different docking programs (Molegro Vritual Docker and AutoDock) in simulation of ligand-receptor interactions for β1 and β2 adrenergic receptors. The exactness of the predicted ligand positions was estimated on the basis of the thirteen known crystallographic structures of the ligand-receptor complexes taken from the PDB database. Significant differences in docking results obtained by using both tested programs were observed. The overall RMSD-based scoring suggests that the procedures and algorithms implemented in AutoDock lead to slightly better results

    Foci of enterobiasis and ascariasis in the Świętokrzyskie Province in 2005-2009

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    Cosmopolitical pinworm (Enterobius vermicularis) and human ascaris (Ascaris lumbricoides) are the most often nematode parasitising in human gastrointestinal tract all over the world. Pinworm are more common in temperate climatic zones of developed countries of Europe and the United States, whereas human ascaris is the most often in developing countries of Central and South America, Africa and Asia. Taking into account the widespread occurrence of enterobiasis and ascariasis and their impact on human health, it is necessary to monitor the incidence of these infestations all over the world. The aim of our work was to present the incidence of parasitic diseases caused by Enterobius vermicularis and Ascaris lumbricoides in Central Poland in the years 2005-2009. The research was conducted in Świętokrzyskie Region, which is an agricultural region of Central Poland. The data concerning the incidence of enterobiasis and ascariasis were collected from Provincial Sanitary-Epidemiological Station in Kielce. Foci of parasitic invasion covered only these cases, when at least two people were affected at the same time and the same place. Taking into account the number and localization of foci of enterobiasis and ascariasis as well as socioeconomic data from Central Statistical Office in Kielce, epidemiologic analysis of parasitic infections in Świętokrzyskie Region was done. In the years 2005-2009 in Świętokrzyskie Region 21 foci of enterobiasis and 16 foci of ascariasis were registered. The most cases of enterobiasis were registered in the year 2005 (12 foci). In examined period, the highest number of ascariasis was noted in 2008 (4 house foci and 2 school foci). The high incidence of parasitic infections of digestive tract in Świętokrzyskie Region is a consequence of several fac tors – most of inhabitants are professionally connected with agricultural and forest environment, what makes higher the risk of soil-transmitted helminthiasis. Moreover, it can be also connected with low sanitary conditions and the lack of appropriate environmental security against soil and water contamination with feces

    A comprehensive analysis of stillbirths influencing factors: Insights from Eastern Algeria’s Maternal Health Study

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    As defined by the World Health Organization, stillbirth refers to the delivery of a baby without signs of life at or after 28 weeks of gestation. This study aims to predict stillbirth occurrences by identifying influencing factors. Interviews were conducted with 786 pregnant women from Eastern Algeria between 2011 and 2015, resulting in a 9.03% stillbirth rate. Utilizing statistical techniques, a predictive model with a 95% confidence interval was developed. Findings indicated a significantly higher likelihood of stillbirths among pregnant women aged over 34 (OR 8.77; 1.70-161.28, p=0.039) and those with a high BMI of (25-30) (OR 1.73; 0.83-3.78, p=0.153) and (30-35) (OR 4.16; 2.04-8.93, p<0.001). Additionally, associations were observed with stress (OR 2.19; 1.15-4.49, p=0.023) and diabetes (OR 2.68; 1.15-5.90, p=0.017). Moderate correlations were noted with meat consumption (OR 0.53; 0.21-1.19, p=0.144), high fish intake (OR 3.02; 1.04-8.62, p=0.039), dairy products (OR 1.81; 0.94-3.67, p=0.085), and sweets (OR 0.47; 0.24-0.88, p=0.021). This study underscores that identifying these risk factors aids in preventing stillbirths, thereby safeguarding the well-being of both mothers and infants. Future research should explore the broader implications of these findings and potential avenues for further investigation

    Cytogenetic analysis of 132 products of conception after spontaneous abortions and fetal death in utero

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    Spontaneous abortion is the most common pregnancy related complication, and more than half of these pregnancy losses are attributed to chromosomal abnormalities. Cytogenetic analysis of spontaneous abortion products provides valuable information on the nature and frequency of chromosomal abnormalities in various populations, their origin and the risk of recurrence in a population. Characterization of chromosomal abnormalities in spontaneous miscarriages or fetal deaths using both classical and molecular cytogenetics to discover the incidence and types of chromosomal aberrations. One hundred and thirty-two samples derived from cases of spontaneous abortion and fetal death in utero were sent for analysis to the medical cytogenetics department of Clermont-Ferrand (France) between January 2011 and December 2015. The various specimens were carefully dissected and cultured to produce karyotypes. In case of culture failure or microbial contamination, a Bac en Beads analysis (BoBs™) has been performed. All abnormalities detected by caryotype or BoBs™ were confirmed by Fluorescent In Situ Hybridization Cytogenetic analysis was successfully performed in 89% of cases (117/132). For normal karyotypes, male-female ratio was 0.81. Twenty-five karyotypes involved chromosomal rearrangement, which represents 21.4% of all cases (25/117). Karyotype failed in 41% of all issues (54/132). A BoBs™ analysis was performed when cultures failed; it was found to be uninformative in 15 cases and revealed 2 chromosomal abnormalities. The average gestational age in aberrant karyotypes was 17.12±8.89 SA, while the average age of mothers was 31.55±6.67 years. The presence of a chromosomal abnormality may explain the cause of the miscarriage, hence, will improve genetic counseling and reproductive planning

    Różnicowanie przy użyciu RAPD-PCR szczepów Candida albicans izolowanych z górnych dróg oddechowych osób w wieku podeszłym przebywających w zakładzie opiekuńczym

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    Elderly people, aged 65 years old or older, are predisposed to fungal infections caused especially by Candida spp., mainly Candida albicans. Among them the most common are respiratory tract candidiasis. The aim of this study was to estimate the genetic diversity by RAPD-PCR of C. albicans isolated from the upper respiratory tract in elderly people from a  care center in order to assess spreading of these strains among people from close population. Among 75 strains, 46 genotypes were defined. 11 clusters comprising 40 (53.33%) isolates at the value of similarity coefficient ≥ 80% were formed. The remaining 35 (46.67%) isolates represented individual genotypes.    Osoby w wieku podeszłym, powyżej 65 roku życia, są szczególnie predysponowane do infekcji grzybiczych wywoływanych przez Candida spp., głównie Candida albicans. Należą do nich najczęściej kandydozy dróg oddechowych. Celem badań była ocena genetycznego zróżnicowania szczepów C. albicans izolowanych z górnych dróg oddechowych osób starszych przebywających w domu opieki społecznej w celu oceny rozprzestrzeniania się tych szczepów wśród osób przebywających w zamkniętej populacji. Uzyskane wyniki wskazują na znaczne zróżnicowanie genetyczne izolatów C. albicans. Wśród 75 szczepów C. albicans stwierdzono 46 genotypów. Wyróżniono 11 typów genetycznych obejmujących 40 (53,33%) izolatów o współczynniku pokrewieństwa ≥ 80% oraz 35 (46,67%) szczepów unikatowych.  

    Wydalanie siarczanu heparanu z moczem zależnie od mikroalbuminurii u pacjentów z cukrzycą

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    The HS is a component of the glomerular filtration barrier, mainly in the form of perlecan and agrin. It has been reported that changes in the amount and concentration of sulfated units of HS are associated with the development of diabetic nephropathy. The lack of or impaired synthesis of HS in GBM leads to the development of microalbuminuria and further excessive urinary protein excretion. To determine the  early stages of diabetic complications we used the quantitative assessment of urinary HS excretion. The aim of the study was searching for differences in urinary HS excretion in patients with diabetes in comparison to the control group, evaluation of the influence of microalbuminuria on urinary HS excretion and evaluation of possible usefulness of urinary HS excretion as a laboratory test for diabetic nephropathy. Results of the conducted research revealed that in patients with diabetes urinary HS excretion in 24-hour urine collection is lower in comparison with the control group. Significant differences were observed in regards to all methods of expression of urinary HS excretion: as a concentration, 24-hour excretion and also as calculation per urine creatinine. Decreased urinary HS excretion in patients with diabetes may result from impaired production or increased degradation of GAG in glomeruli in the course of hyperglycemia. In our research we observed a significant negative correlation between urinary HS excretion and microalbuminuria and positive association with eGFR values. The presented data indicate that determining HS can be a valuable marker in the diagnosis of diabetic nephropathy; however, details of the preanalytic and analytic phase need to be processed. In the clinical aspect further investigations are required on a greater number of cases considering many factors such as the type of diabetes, administered treatment and the duration of the disease.Siarczan heparanu (HS) stanowi składnik bariery filtracyjnej kłębuszków nerkowych, głównie w postaci  perlekanu i agryny. Dowiedziono, że zmiany w koncentracji i ilości grup siarczanowych HS są powiązane z wystąpieniem nefropatii cukrzycowej. Brak lub upośledzona synteza HS błony podstawnej prowadzi do rozwoju mikroalbuminurii i dalej do nadmiernego wydalania białek z moczem. Do określenia powikłań cukrzycy posłużono się więc oceną ilościową wydalania HS z moczem. Celem pracy było: poszukiwanie różnic w wydalaniu HS z moczem u pacjentów z cukrzycą w porównaniu z grupą kontrolną,  ocena zależności pomiędzy mikroalbuminurią  a wydalaniem HS z moczem oraz ocena ewentualnej przydatności oznaczania wydalania HS z moczem jako testu laboratoryjnego w  nefropatii cukrzycowej. Wyniki przeprowadzonych badań wykazały, że u osób cierpiących na cukrzycę wydalanie HS z moczem w zbiórce 24-godzinnej jest niższe niż w podobnej pod względem płci i wieku grupie osób zdrowych. Istotność statystyczną uzyskano dla wszystkich sposobów wyrażania wydalania HS z moczem tj. jako stężenia, wydalania 24-h i po przeliczeniu na kreatyninę. Obniżone wydalanie HS z moczem u pacjentów z cukrzycą sugeruje, że prawdopodobnym powodem tych zmian może być spadek produkcji lub przyspieszona degradacja GAG w strukturach błony kłębuszka, obserwowana podczas hiperglikemii. W naszych badaniach uzyskaliśmy istotną statystycznie ujemną korelację między wydalaniem HS a mikroalbuminurią, widoczną w zakresie wszystkich sposobów wyrażania wydalania HS z moczem, oraz dodatnią korelację z wartościami eGFR. Przedstawione dane wskazują, że oznaczanie HS może być przydatnym markerem w diagnostyce nefropatii cukrzycowej, wymaga jednak opracowania szczegółów fazy przedanalitycznej i analitycznej. W aspekcie klinicznym konieczne są obserwacje na większej liczbie pacjentów z uwzględnieniem szeregu czynników, takich jak: typ cukrzycy, stosowane leczenie oraz czas trwania choroby

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