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Revitalization of English Teacher Education: An Effort To Improve Ther Quality of Students' Learning In English
Moderate Concentrations of TNF- α Induce BMP-2 Expression in Endothelial Cells
Tumor necrosis factor-alpha (TNF-α) is best-known as a potent pro-inflammatory cytokine involved in many
cardiovascular diseases. During vascular calcification, TNF-α has been reported topromote osteogenic differentiation of
human vascular smooth muscle cells (VSMC) and mesenchymal stem cells (MSCs). In contrast, there is alack of data
reporting the osteoinductive effect of TNF-α in endothelial cell. In this study, experiments were performed to investigate
and determine theoptimum dose of TNF-α that induces expression of the osteoinductive factor bone morphogenetic protein
(BMP-2) in endothelial cells. Human vein endothelial cells were treated with TNF-α at doses of 0, 2, 5, 10, or 20 ng/mL
for2, 8, or 24 h time intervals. BMP-2 cell expression was evaluated using immunocytochemistry staining by calculating
the percentage of BMP-2 positive cells. Apoptosiswas determined by counting the number ofpyknoticcells. In this study,
we found that the optimum dose of TNF-α thatinduces BMP-2 expression in endothelial cells was 5 ng/mL at the 8 h time
interval. Lower (2 ng/mL) orhigher (10 and 20 ng/mL) concentrations of TNF-α had minimal effects on BMP-2 expression.
Moreover, higher concentrations of TNF-α treatment (10 and 20 ng/ml) at8 h and 24 h increased the presence of pyknotic
endothelial cells, which representthefinal stage of apoptosis
PEER REVIEW (KARYA ILMIAH): Community Participation on Flood Management in Hulu Sungai Tengah District South Kalimantan Province
Is stabilization of organic matter in the raised-bed soils of tidal swamplands influenced by “peliburan” – mixing muddy silt-clay minerals with organic matter?
Stabilization of organic matter in raised-bed soils of tidal swamplands reduce greenhouse gas emissions and influence organic matter contents. Farmers in tidal swamplands annually conduct "peliburan" - deposed organic matter mixed with muddy silt-clay minerals from sunken-beds and then piled onto the raised beds. This experiment aimed to study the influence of "peliburan" on the stabilization of organic matter in the raised-bed soils. Results of this study showed that organic carbon content did not change for the raised-bed aged 2-15 years, but decreased significantly for the raised bed aged 26 years. Organic carbon contents in the raised-beds were controlled by soil surface properties such as clay content, exchangeable Ca and Al-Fe oxides. This study showed that “peliburan” could maintain organic matter contents of the raised-bed soils for ≤ 15 years