Repo Dosen ULM (Universitas Lambung Mangkurat)
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    Moderate Concentrations of TNF- α Induce BMP-2 Expression in Endothelial Cells

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    Tumor necrosis factor-alpha (TNF-α) is best-known as a potent pro-inflammatory cytokine involved in many cardiovascular diseases. During vascular calcification, TNF-α has been reported topromote osteogenic differentiation of human vascular smooth muscle cells (VSMC) and mesenchymal stem cells (MSCs). In contrast, there is alack of data reporting the osteoinductive effect of TNF-α in endothelial cell. In this study, experiments were performed to investigate and determine theoptimum dose of TNF-α that induces expression of the osteoinductive factor bone morphogenetic protein (BMP-2) in endothelial cells. Human vein endothelial cells were treated with TNF-α at doses of 0, 2, 5, 10, or 20 ng/mL for2, 8, or 24 h time intervals. BMP-2 cell expression was evaluated using immunocytochemistry staining by calculating the percentage of BMP-2 positive cells. Apoptosiswas determined by counting the number ofpyknoticcells. In this study, we found that the optimum dose of TNF-α thatinduces BMP-2 expression in endothelial cells was 5 ng/mL at the 8 h time interval. Lower (2 ng/mL) orhigher (10 and 20 ng/mL) concentrations of TNF-α had minimal effects on BMP-2 expression. Moreover, higher concentrations of TNF-α treatment (10 and 20 ng/ml) at8 h and 24 h increased the presence of pyknotic endothelial cells, which representthefinal stage of apoptosis

    Is stabilization of organic matter in the raised-bed soils of tidal swamplands influenced by “peliburan” – mixing muddy silt-clay minerals with organic matter?

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    Stabilization of organic matter in raised-bed soils of tidal swamplands reduce greenhouse gas emissions and influence organic matter contents. Farmers in tidal swamplands annually conduct "peliburan" - deposed organic matter mixed with muddy silt-clay minerals from sunken-beds and then piled onto the raised beds. This experiment aimed to study the influence of "peliburan" on the stabilization of organic matter in the raised-bed soils. Results of this study showed that organic carbon content did not change for the raised-bed aged 2-15 years, but decreased significantly for the raised bed aged 26 years. Organic carbon contents in the raised-beds were controlled by soil surface properties such as clay content, exchangeable Ca and Al-Fe oxides. This study showed that “peliburan” could maintain organic matter contents of the raised-bed soils for ≤ 15 years

    Payudara & Kelainannya

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    Peer Review: Keragaan Biofisik Agroekosistem Kalimantan Selatan

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    Repo Dosen ULM (Universitas Lambung Mangkurat)
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