eKhSACIR інституційному репозитарії Харківської державної академії культури
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    Couche G ou couche tertiaire : quel nom pour la couche interne des fibres du bois de tension ?

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    Couche G ou couche tertiaire : quel nom pour la couche interne des fibres du bois de tension ?. 11es Journées du Réseau Français des Paroi

    Identification of genomic regions associated with caecal microbiota composition in a broiler line

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    Identification of genomic regions associated with caecal microbiota composition in a broiler line . 10th European Symposium on Poultry Genetic

    Synchrotron label free multimodal imaging to explore lipid droplets in living cells

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    National audienceImaging native intracellular compartments in living cells is still challenging. The use of tagged proteins often modifies the morphology and the smooth running of the organelles. Methods based on intrinsic fluorescence of molecules upon excitation by deep-UV illumination are emerging for living cell imaging. We used deep UV light from synchrotron SOLEIL DISCO beamline to settle up and perform protein absorption imaging on single living yeasts. This technique was combined with transmission fluorescence microscopy. The visualization of contrasted signals inside the cells revealed chemical heterogeneity at the subcellular level. Fluorescence microscopy showed organelles with low fluorescence after 270-nm excitation. We could discriminate two populations, with high or low absorbance after 270-nm excitation. The first population was identified as lipid droplet using TMA-DPH and DPH lipid stains. Surprisingly, the second population corresponds to vacuoles. Combining deep UV fluorescence and absorption, two emerging techniques, enabled the acquisition of information on both organelle organization and chemical composition

    LIDeOGraM : an interactive evolutionary modelling tool

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    Building complex models from available data is a challenge in many domains, and in particular in food science. Numerical data are often not enough structured, or simply not enough to elucidate complex structures : human choices have thus a major impact at various levels (data and model structuration, choice of representative scales, parameter ranges, uncertainty assessment and management, expert knowledge). LIDeOGraM is an interactive modelling framework adapted to cases where numerical data and expert knowledge have to be combined for building an ecient model. Exploiting both stand-alone evolutionary search and visual interaction with the user, the proposed methodology aims at obtaining an accurate global model for the system, balancing expert knowledge with information automatically extracted from available data. The presented framework is tested on a real-world case study from food science : the production and stabilisation of lactic acid bacteria, which has several important practical applications, ranging from assessing the ecacy of new industrial methods, to proposing alternative sustainable systems of food production

    Make or Buy: Political hazards and the choice of governance of municipal services

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    We study the link between the choice of governance and political hazards using municipal school canteens. We provide evidence that in politically contested cities, mayors are more likely to choose to “make” rather than “buy” to avoid challenge of the contract with a private partner. A higher probability of losing office – proxied by close victory margins and political concentration – increases the probability to “make”. We also provide evidence that in cities where the potential challenge on sustainable supply is high, mayors tend to “make” also to signal trustworthiness to the voter. A higher presence of ecologist’s citizens and alternative supply organizations – proxied by the presence of Agenda 21 and the presence of alternative and organic food shops – increases the probability to “make”

    How “fundamental knowledge” supports the cropping system redesign by farmers?

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    Re-designing cropping systems to move towards agroecology leads farmers to implement practices which involve biological processes, sometimes qualified as “knowledge-intensive”, as they involve the renewal of agronomic principles and numerous interactions between the systems’ components and their regulations. Agronomists have developed an abundance of models, which encapsulate partial knowledge on systems’ functioning, but these appear to be seldom used by farmers. By contrast, several studies recognize the value of exchanging specific and fundamental knowledge with farmers in relation to technical change processes. This paper discusses how fundamental and generic knowledge acquires an agronomic sense and is reinvested in the action of farmers through their technical changes. We performed an inductive case study of step-by-step cropping system re-design situations. We combined individual interviews with farmers re-designing their cropping-system, and facilitated farmers meeting about a shared technical problem. From full transcripts, we identified each new element of knowledge and its reformulation, its relation to action mentioned by farmers. The focus of our analysis concerns the knowledge which made possible to develop action strategies when farmers were facing hindrances in continuing their technical changes. Our findings concern the specific fundamental knowledge actually mobilized, and the processes of its linkage with action through contextualization. We conclude by suggesting that farmers alternate between systematic and systemic thinking about the biological processes at play in their own situation. This has practical implications for agronomists wishing to support such re-design processes, and provides an insight on how farmers’ experiments might be combined to fundamental scientific knowledge on agroecosystems components to enhance cropping system redesign

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    eKhSACIR інституційному репозитарії Харківської державної академії культури
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