eKhSACIR інституційному репозитарії Харківської державної академії культури
Not a member yet
7410 research outputs found
Sort by
Méta-analyse de données publique transcriptomique pour comprendre et phenotyper la composition corporelle des bovins
No full textSession 4: Nutrition to improve milk and meat qualityNational audienc
Biochem-Env: a platform of biochemistry for your research in functional ecology
No full textNational audienc
Effets de l’exposition maternelle aux nanoparticules d’or par ingestion pendant la gestation, sur le développement foeto-placentaire et la fonction placentaire, dans un modèle lapin
No full textNational audienc
Structural study of lipid droplet using synchrotron label-free multimodal imaging
No full textNational audienceImaging intracellular compartments, their dynamics and interactions in living cells, remains challenging. Using various lipid droplet (LD) protein markers, we revealed inter LD heterogeneity at single cell level, due to LD specific geolocalisation and enzymatic equipment. Even so, using tagged proteins or vital probes could modify the morphology and the smooth running of the organelles. Methods based on intrinsic fluorescence of molecules upon excitation by deep ultra violet (DUV) illumination are thus emerging for living cell imaging. We used DUV from synchrotron radiation to perform autofluorescence and transmittance imaging on single living yeast. The contrasted signals inside the cells revealed chemical heterogeneity at the subcellular level. Microscopy showed organelles with low auto-fluorescence after DUV illumination. We distinguished two populations, with high or low transmittance. The first population corresponded to vacuoles and the second to LDs. LDs appeared as heterogeneous well-organized structures with a low transmittance zone on the surface and a high transmittance core. We propose that the low transmittance ring and the high transmittance core correspond to ergosterol and triacylglycerol-containing structures, respectively. The conclusions we drawn using DUV imaging were confirmed by experiments performed using soft X ray imaging on cryofixed cells. Synchrotron label free imaging paves the way for efficient structural and dynamic studies of LDs and other organelles
A porphyromonas-Pseudomonas aeruginosa "pas de deux" in the airways microbiota of cystic fibrosis patients
No full textInternational audienc
