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Identification of Probiotic Bacteria Isolated from Domestic Chickens (Gallus domesticus) Using the 16S rRNA Gene Method
No full textThe intestines of domestic poultry (Gallus domesticus) are one of the potential sources of probiotic bacteria that can produce antibacterial agents. The objective of this study was to identify the types of probiotic bacteria obtained from the digestion of domestic poultry using the molecular analysis method of 16S rRNA gene sequencing. Observations were conducted on colony morphology, gram staining, biochemical tests, and antibacterial activity using the diffusion agar method. Molecular analysis of DNA extraction was carried out, followed by the amplification of samples using a 16S rRNA universal primer. Dielectrophoresis and sequencing were performed on the 16S rRNA gene. The identification of morphological observations, gram staining, and biochemical tests showed that probiotic bacteria isolates, including Gram-positive, rod-shaped, rounded colony form, flat elevation, entire nonmotile edge, and catalase-negative, could ferment all carbohydrate content in the TSIA medium. The antibacterial potential was also found in probiotic bacteria, as evidenced by the inhibition zone formed in the test. The results of the bacterial gene sequences of PaTa5 probiotic bacterial isolates had a similarity of 98.37 with Lactobacillus plantarum. These findings indicated the presence of some bacteria species that have antibacterial activity in the intestines of domestic chickens (Gallus domesticus). © 2023,Journal of World''s Poultry Research. All Rights Reserved
Suitability of Inguinal and Axillary Sites for Temperature Measurement Using Digital Thermometers: A Comparison with Rectal Thermometry in Broiler Chicken
No full textCore body temperature is one of the physiological parameters that must be assessed during the monitoring of the thermic and or health status of broiler chickens. In this regard, cloacal thermometry is a standard method used for temperature measurement although it has many drawbacks. This study was conducted to explore the suitability of other anatomical sites for temperature measurement using a digital thermometer. This was a single-factor experiment that considered the anatomical site as the main factor with three levels (treatments), including cloacal (DTtcloacal), axillary (DTtaxillary), and inguinal (DTtinguinal) sites. Out of 84 broiler chickens, a total of 28 chickens were randomly selected for temperature measurement. The temperature was measured for each anatomical site, and the readings were analyzed using appropriate statistics. The cloacal site had the highest mean temperature (41.40 ± 0.17°C), while the lowest mean value was observed for the axillary site (41.12 ± 0.19°C). There was no significant difference between the mean cloacal and inguinal temperatures. The cloacal and inguin.al temperature readings were significantly correlated. The results for the cloacal and inguinal temperature measurements revealed a non-significant bias. The agreement interval between these two methods was sufficiently lower than the maximum acceptable difference between the anatomical sites. Both cloacal and inguinal temperature measurements had similar median points. The results indicated an underestimation of the temperature readings for the axillary site compared to those of the other sites. In conclusion, this study has revealed that the application of a digital thermometer using the inguinal site gives temperature readings that are similar to those of the conventional cloacal method. © 2023, Journal of World''s Poultry Research. All Rights Reserved
Effects of Replacing Maize by Proso Millet on Performance of Broiler Chickens
No full textThe continual rise in the cost of poultry feed ingredients, the fluctuations in price and the comparatively insufficient maize supply have prompted a search for less expensive alternatives. This research study was carried out to investigate the impact of a partial or total replacement of maize with proso millet on performance parameters of broiler chickens, including live body weight, feed conversion ratio, mortality rate and carcass yield. An experiment was carried out using 160 one-day-old broiler chicks of a commercial breed. The chicks were randomly assigned to 5 groups of 32. They consumed different isoprotein and isocaloric diets in which maize was replaced by proso millet at 0, 25, 50, 75, or 100 inclusion rates as T1, T2, T3, T4 and T5. Results showed that all broiler chickens fed on diets containing different rates of millet instead of maize significantly improved live body weight, feed conversion ratio, and carcass yield for females and males compared to T1. Additionally, it was observed that there was a significant decrease in the relative weight of the liver for females and males compared to T1. The use of millet in diets did not negatively affect the broilers� health, and the mortality rate was low throughout the experiment. These results confirmed that maize could be replaced by proso millet in broiler chicken diets up to 100. © 2023, Journal of World''s Poultry Research. All Rights Reserved
Effects of Commercial and Homemade Extenders on Post-thaw Sperm Quality and Fertility of Semen from Ethiopian Indigenous Horro Chicken Breed
No full textCryopreservation of spermatozoa represents an important strategy for in vitro programs designed for the conservation of the genetic material of livestock populations. The objective of this study was to evaluate the effects of homemade tris-egg yolk-based and commercial poultry semen extenders on post-thaw sperm quality, fertility, and hatchability of semen from the Ethiopian Indigenous Horro chicken breed. A total of 30 roosters were used for semen collection, and 160 adult hens were inseminated artificially. The collected, qualified, and pooled semen samples were divided into three groups. Each semen sample was diluted with a homemade tris-egg yolk-based extender, Dimethyl-formamide commercial extender, and Commercial Beltsville Poultry Extender. Each extended semen was further divided into 20 sterile tubes as replicates. The extended semen samples were cryopreserved in liquid nitrogen using standard procedures. Changes in post-thaw spermatozoa mass and progressive motility, in vitro viability, morphological abnormality, fertility, and hatchability were evaluated. The post-thaw evaluation showed a decrease in the mass and progressive motility, morphologically normal spermatozoa, and an increase in dead spermatozoa and spermatozoa with bent necks, compared to fresh semen. There were significant differences in progressive sperm motility, motility, and in vitro viability between commercial and homemade cryoprotectants. However, no significant difference was observed in mass motility across the extenders. The commercial Dimethyl-formamide extender was found to be the most suitable regarding the proportion of morphologically normal sperm and in vitro viability rate of cryopreserved sperm samples. There were no significant differences across all treatments in terms of fertility and hatchability rate. However, there was a significant difference between the control treatment and commercial extenders in terms of fertility and hatchability. The findings indicated favorable outcomes for a tris-egg yolk-based extender that was prepared locally with regard to the cryopreservation of poultry semen. Additional investigations are recommended to enhance the fertility and hatchability of the semen. © 2023, World''s Veterinary Journal. All Rights Reserved
Protective Role of Rosa damascena Miller Hydroalcoholic Extract on Oxidative Stress Parameters and Testis Tissue in Rats Treated with Sodium Arsenite
No full textRegarding the strong antioxidant properties of Rosa damascene extract, this study aimed to investigate the protective role of Rosa damascene Miller hydroalcoholic petal extract on oxidative stress parameters and testis tissue in rats treated with sodium arsenite. To this end, 30 male rats were divided into five groups, including control, positive control (treated with arsenite), and three groups of patients affected by sodium arsenite with 150 mg/kg, 300 mg/kg, and 450 mg/kg Rosa damascene extract for 34 days by gavage. The animals were then anesthetized, and the blood samples were collected from the heart. The left testis was removed for histopathological studies. The findings revealed that Sodium arsenite in the positive group caused a significant reduction in TAC, testosterone, and serum Luteinizing hormone (LH) and a significant increase in serum Malondialdehyde. In addition, there was no statistically significant difference among the groups regarding the amount of Follicle-stimulating hormone (FSH). Moreover, the consumption of Rosa damascene extract with sodium arsenite caused a significant increase in testosterone, LH, and FSH compared to the positive control group. Histopathological results showed that in the experimental group receiving a dosage of 300 mg/kg b.w and the control group, the number of sperm tubes increased, and the germinal epithelium�s thickness was appropriate. Daily treatment with Rosa damascene extract with a dosage of 300 mg/kg b.w for 34 days could improve the changes caused by sodium arsenite and reduce Malondialdehyde levels. Thus, it seems that Rosa damascene hydroalcoholic extract can effectively improve the male reproductive system�s function. © 2023, World''s Veterinary Journal. All Rights Reserved
Toxic Effects of Nanographene Oxide on Testes of Rats
No full textThe current study aimed to examine the effects of nanographene oxide on the testes. A total of 48 male albino rats were randomly divided into 6 groups. The first, second, third, fourth, and sixth groups were treated with graphene oxide nanopowder at 20, 30, 40, 50, and 60 mg/kg concentrations, respectively. The sixth group was considered the control group. The results indicated a significant decrease in the average testis weight of rats treated with different nanographene oxide dosages, compared to the control group. There was also a significant decrease in the level of FSH and testosterone of treated rats with nanographene oxide, while there was no significant difference in the level of LH hormone when compared to the control group. The histological examination of the testes in the treated rats indicated hemorrhage, decreased sperm count, decreased thickness of the tubular epithelium, dissociation of connective tissue between the seminiferous tubules, in addition to hematological congestion, necrosis of the tubular epithelium, divergence of the seminal tubules, absence of sperm, shattering of the seminal tubule wall and degeneration sperm-forming cells and edema formation. Using the transmission electron microscope, the findings revealed a range of cellular changes, such as the presence of two-headed spermatids, the destruction of the nucleus membrane, spermatoblasts, the destruction of the cell membrane, and the denting of the nucleus membrane. It can be concluded that the nanographene oxide at 20-60 mg/kg concentrations can have harmful effects on spermatogenesis and normal function testis in rats © 2023, World's Veterinary Journal.All Rights Reserved
Antibiotic Resistance of Escherichia coli and Salmonella Species Isolated from Table Eggs in Morocco
No full textThe development of antimicrobial resistance has become a severe global public health emergency. Foods of animal origin are considered possible drivers of resistant bacteria, including Escherichia coli (E. coli) and Salmonella spp. It is associated with the indiscriminate use of antibiotics, resulting in the inability to treat patients infected with antibiotic-resistant pathogens and a high risk of transmission of these resistant pathogens. The current study aimed to determine the prevalence and antibiotic resistance of E. coli and Salmonella spp. in raw table eggs in Morocco. A total of 870 table eggs resulting from 290 samples (3 eggs = 1 sample), were purchased from ambulatory sellers, street vendors, kiosks, and neighborhood markets from different cities in Morocco and transferred to the laboratory in the Hassan II Agronomy and Veterinary Medicine Institute of Rabat, Morocco. The egg shells and contents were tested separately then the isolation and identification of bacterial pathogens were performed according to the Moroccan Standard Norms. The bacterial isolates were tested for susceptibility to six commonly used antibiotics, namely nalidixic acid (30 μg), kanamycin (30 μg), gentamycin (15 μg), ciprofloxacin (15 μg), tetracycline (30 μg), and amoxicillin (10 μg). The findings revealed that 38 samples (13) tested positive for E. coli of which 9 were on egg shells, and 4 were in egg content, while for Salmonella enteritidis (S. enteritidis), 5 samples (2) tested positive and only in the egg contents. Escherichia coli showed the highest resistance to amoxicillin, followed by tetracycline and nalidixic acid with 92.10, 84.21, and 50, respectively, and was sensitive to ciprofloxacin (84.21), kanamycin (65.79), and gentamicin (60.54). Salmonella enteritidis had the highest resistance against tetracycline (80), followed by ciprofloxacin and nalidixic acid with 40 each. The highest sensitivity rates of S. enteritidis were for gentamicin, amoxicillin, and kanamycin at 80, 80, and 40, respectively. Given that these resistant bacteria could potentially be transferred to humans through eggs or egg products, it is necessary to employ strict hygiene measures and provide a wise and legal use of antibiotics in animal breeding. © 2023, World''s Veterinary Journal. All Rights Reserved
A Retrospective Study on Dairy Cattle Mortality Patterns in Two Farms of South-eastern Botswana
No full textGenerally, high mortalities of dairy cattle due to infectious and non-infectious diseases cause huge economic losses, unprofitability, and low productivity in the dairy industry. The present study aimed at determining the mortality rates, their causes, and risk factors among 1779 cattle at two dairy farms belonging to the Department of Agricultural Research, Botswana. An 8-year retrospective study was conducted using farm records during 2005-2012. Monthly and annual records of the farms were examined regarding the total dairy cattle population, sex, breed, age, cattle deaths, and causes of death. Mortality was calculated from the total cattle population and expressed as a percentage, and it was analyzed with respect to farm, breed, age, sex, year, season, and mortality causes. The overall mortality rate was 8.5. The semi-intensively managed Farm II, as well as young stock (<12 months old), and males recorded significantly higher mortalities than their counterparts. Dairy crosses of pure exotic and indigenous Tswana cattle had higher mortalities than the Friesians and Jerseys, and the wet season accounted for over 70 of the total deaths. Only two years (2010 and 2012) out of the 8-year study period had a mortality rate < 5. Notably, 28.1 of mortalities with a known cause were due to heartwater disease (n = 57), but most deaths (62.3) were due to unknown causes. In conclusion, to improve farm herd health and husbandry practices, more efforts should be devoted to preventing heartwater and mortalities in young stock and male animals, particularly during the hot-wet season. © 2023, World''s Veterinary Journal. All Rights Reserved
Knowledge, Attitudes, and Practices of Red Meat Handlers in Moroccan Slaughterhouses
No full textMeat handlers are vectors of pathogens in slaughterhouses and can play a major role in the microbiological contamination of meat. The level of knowledge of meat handlers in slaughterhouses is a critical factor in food safety. Good hygienic practices in the slaughterhouse are required to reduce the risk of microbiological contamination while handling meat. This study evaluated workers� knowledge, attitudes, and practices in four municipal slaughterhouses in Morocco. A total of 267 employees were evaluated using a structured survey. The results showed that workers had acceptable knowledge and practices, and their attitudes were very satisfactory, averaging 52.87, 50.9, and 63.07, respectively. A positive correlation between the workers� level of knowledge and education was found in all studied slaughterhouses. Similarly, the results indicated a positive correlation between knowledge and attitudes at Meknes and Kenitra slaughterhouses. The impact of the studied sociodemographic characteristics may vary from one slaughterhouse to another. In conclusion, the study suggested that although the knowledge, attitudes, and level of practice of food handlers were very satisfactory, some aspects related to the control of the health status of the handlers and personal protective equipment had to be underlined. Ongoing food safety training should become mandatory to enhance food safety in the slaughterhouses of study locations © 2023, World's Veterinary Journal.All Rights Reserved
THE QUALITY OF FERMENTED RICE STRAW WITH Trichoderma viride INOCULUM
No full textRice straw has several nutritional weaknesses, namely its high silica and lignin content, and its low level of protein, minerals and vitamins, so the impact on digestibility is also low. Aim of present study was to evaluating nutritional efficacy of rice straw after fermentation with Trichoderma viride. The study was conducted by using complete random design. There were three different treatments with four replicates for each treatment. Fermented rice straws were treated with varying concentrations of Trichoderma viride inoculum as follows; 0.5 (T1), 1 (T2), and 1.5 (T3). Fermented rice straw's nutrients, including dry ingredients, organic material, crude fiber, crude protein, dry matter digestibility coefficients, and organic matter digestibility coefficients were measured in this study. T3 (1.5 of T. viride) treatment performed a proper nutrient, with 80.02 dry ingredients, 80.03 organic materials, 31.68 crude fiber, 5.72 protein, 38.46 dry matter digestibility coefficient, and 61.05 organic matter digestibility coefficient. In conclusion, using 1.5 Trichoderma viride to improve the quality of rice straw, as stimulator of fermentation process can be efficient in ruminant or non-ruminant nutrition. © (2023). All Rights Reserved