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The Effects of Different Concentrations of Vitamin D3 on Immunological Parameters of Immunosuppressed Rats Induced
No full textVitamin D3 receptor is expressed in several types of immune cells suggesting that Vitamin D3 could have immune regulatory roles. The current study was conducted to investigate the role of Vitamin D3 in reducing the toxicity of the cisplatin on some Immunological parameters in the rat model. The current experiment was conducted on 80 adult white male rats within the age range of 9-12 weeks. The animals were divided into eight groups (10 animals in each group). The control group was dosed with the physiological solution until the end of experiment (C). Rats in the second treatment were injected with cisplatin (2 mg/kg, T1). Rats in the third (T2), fourth (T3), and fifth (T4) groups were injected with cisplatin at a concentration (2 mg/kg) and received Vitamin D3 at levels of 5000 IU, 10,000 IU, and 15,000 IU, respectively. The rats in the sixth (T5), seventh (T6), and eighth (T7) groups were subjected to Vitamin D3 at concentrations of 5000 IU,10,000 IU, and 15,000 IU, respectively. At the end of the experiment, which lasted 21 days, the animals were anesthetized, their weights were recorded, and blood samples were collected. The findings revealed a significant elevation in the levels of interleukin-12, tumor necrosis factor-alpha, C-reactive protein, lymphocyte percentage, monocyte percentage, and eosinophil percentage within group T1 compared to the control and other treatment groups that received Vitamin D3. The average percentage of white blood cells and neutrophils in group T1 was significantly lesser than other groups. It can be concluded that supplementation of different Vitamin D3 levels (5000-10,000 IU) have positive influences on the immunological parameters of immunosuppressed rats. © (2024) Baishideng Publishing Group Inc. All rights reserved
The Role of Neutrophils and NETosis in Local Immunity of Feline Inflammatory Aural Polyps
No full textFeline inflammatory aural polyps are abnormal growths that can occur in the ear canals of cats, particularly in the middle ear. These polyps are frequently linked to persistent inflammation and can result in a range of ear-related complicated pathologies. The etiology is multifactorial. The purpose of the research was to study the cytology of an inflammatory polyp in a cat and to study the role of neutrophils and their mechanisms on the formation of extracellular protective traps by neutrophils (NETs). A 4-year-old, female spayed, Scottish fold cat, weighing 3.5 kg sent to a veterinary clinic (Mirra-Vet, Kyiv, Ukraine). Clinical, otoscopic methods, and laboratory methods of cytological diagnostics were used for the research. At the onset of the clinical investigation, exudate discharge from the ear and a painful response were observed. Upon detailed otoscopy, a polyp in the ear canal was diagnosed. An increase in the number of leukocytes (23.2×109/L), their absolute content, and an increase in the percentage of neutrophils (48.2 ) in the leukogram. Assessing the capacity of neutrophils to generate NETs (Neutrophil Extracellular Traps) was determined after samples were collected using a cytologic brush. Cytological analysis of samples from the inter-tragic incisure area highlighted a significant presence of neutrophils, forming extracellular protective traps. The results revealed free NETs in separate areas of the slides. The findings indicated the formation of cooperative groups among neutrophils, other phagocytes, and epithelial cells, along with slender nuclear streaks. During the treatment (Otoflox, 2 drops per ear), the inflammatory reaction disappears, polyp size decreases, exudative reactions decrease, and neutrophil activity decreases. After 3 days of treatment, the animal's condition improved. The ear was clean without sulfur and lesions. The complete treatment course spanned 7 days. During the treatment, the inflammatory reaction disappeared, polyp size decreased, exudative reactions decreased, and neutrophil activity decreased. Experimental studies have shown that during the inflammatory reaction in the ear, protective mechanisms of local immune defense are activated. Activated neutrophils perform their function through phagocytosis and the formation of NETs. These studies contribute to supplementing the data on the immunopathological mechanisms of feline inflammatory polyps. © The Author(s) 2024. All Rights Reserved
Antibody Response of Mice to Bali Isolate of Canine Parvovirus Propagated in Madin-Darby Canine Kidney Cell Culture
No full textCanine parvovirus (CPV) infection is still common among dogs, leading to severe disease with high mortality. The potential of a local isolate of CPV as an effective vaccine to prevent the disease warrants investigation. This study aimed to determine the antibody response in mice against a Bali isolate of CPV propagated in the Madin-Darby Canine Kidney (MDCK) cell culture. The virus was purified using polyethylene glycol (PEG)-6000 and mixed with an Aluminum hydroxide adjuvant. Fifteen 7-week female mice were divided into three treatment groups: treatment group 1 (PEG-purified virus and Adjuvant), treatment group 2 (crude unpurified virus and adjuvant), and treatment group 3 (adjuvant without virus), with five replicates per group. The Bali isolate of CPV was successfully replicated in MDCK cells, achieving a titer of 210-211 hemagglutination (HA) units after eight serial passages through the cell culture. The virus was confirmed as CPV by immunocytochemistry test using a monoclonal antibody and hemagglutination inhibition (HI) test using chicken anti-CPV polyclonal antibody. Following the first immunization, the antibody endpoint titer in mice immunized with PEG-purified CPV (5.6) was significantly higher than those immunized with crude unpurified CPV (4.2) and adjuvant without CPV (1.4). Similarly, after the second immunization, the antibody endpoint titer in mice immunized with PEG-purified CPV (7.6) also remained significantly higher than those immunized with crude unpurified CPV (6.4) and adjuvant without CPV (0.8). Significant increases in antibody endpoint titer were observed after the second immunization in mice immunized with PEG-purified CPV and crude unpurified CPV, but not in those given adjuvant without CPV. The Bali isolate of CPV propagated in MDCK cell culture induced a robust antibody response in mice, suggesting it’s a potential as an alternative vaccine candidate for preventing CPV infection in dogs
Moment Gradient Factor Verification for Selected Monosymmetric Beams under Linear Moment Gradients
No full textThe reuse of doubly symmetric beams by converting them into monosymmetric section beams offers some promising outcomes and has potential for mitigating carbon footprint of structures. However, due to their complexity monosymmetric sections must be used in a configuration that allows the monosymmetry effects to act beneficially. Although the South African design standard for hot-rolled steel does not provide any guidance on the design of monosymmetric beams, the Southern African steel construction handbook provides a formula for determining the critical elastic buckling moment for monosymmetric beams. This guidance implies that the moment gradient factor used for doubly symmetric sections can be used on monosymmetric sections as well. The aim of the study was to verify the validity of this approach of extending the moment gradient factor used for doubly symmetric beams to monosymmetric beams for two specific types of monosymmetric sections. It was found that although this approach appears to be justified for monosymmetric members in single curvature bending it may produce unconservative values of the critical buckling load in double curvature bending between restraint points. The level of un-conservatism also varies for different spans of the same member. This makes it difficult to specify a single moment modification factor value for these cases. The sensitivity in terms of load reduction observed for double curvature bending case was different for the two members examined with this attributed to differences in how the shear centre moves relative to the centroid. It is recommended that the critical buckling load for monosymmetric sections be determined on a case specific basis for members in double curvature from linear moment gradients. Under single curvature bending the moment gradient factor for doubly symmetric members appears to give acceptable predictions of the critical load
Epidemiological Investigation of Eimeria Species and Effectiveness of Togolese Medicinal Plants Used Against Chicken Coccidiosis
No full textEimeria species cause coccidiosis, a poultry disease that occurs worldwide. Infection is linked to decreased feed efficiency and body weight increase. The present study aimed to assess the prevalence of coccidian species in Togolese poultry farms and evaluate the anticoccidial efficacy of three local medicinal plants. From July to September 2023, two hundred and ninety-five fecal samples were randomly collected using a cross-sectional observational study in the maritime region of Togo, specifically in Vo, Lacs, Zio, and Grand-Lomé districts. Data on risk factors were collected through an interview with the poultry farmers. All fecal samples collected were subjected to Eimeria oocyst counting using the standard McMaster technique. The anticoccidial activity of the extract of Azadirachta indica leaves Carica papaya seeds, and Sarcocephalus latifolius roots in a completely randomized design was evaluated on 23-day-old male Isa brown chicks infected with 30.104 oocysts. Body weight gain, feed efficiency, lesion score, proportion of bloody droppings, anticoccidial index, and excretion of coccidia oocysts were assessed. The results revealed an overall prevalence of 39.66 (117/295) for coccidiosis, with 75 of positive samples having fewer than 10, 000 oocysts/g. The logistic regression test indicated that the interval between two anticoccidial prophylaxis applications, age, management, and breed were significant risk factors associated with coccidial infection, with young chicks (≤ 8 weeks) being 5.66 times more susceptible than those older ones (8 weeks) with 0.86 as an odd ratio. Six Eimeria species were identified, with E. maxima (54.17), E. brunetti (33.33), and E. tenella (25) being the most common. The anticoccidial efficacy of Azadirachta indica leaves, Carica papaya seeds Sarcocephalus latifolius roots extract, and amprolium was demonstrated by a reduction in lesion scores, bloody diarrhea, and oocysts per gram in feces (OPG) as well as an improvement in body weight, feed conversion ratio, and production efficiency factor when compared to infected and untreated groups. The anticoccidial index was marked in the chickens treated with Sarcocephalus latifolius roots extract (170) and amprolium (176). The findings of this large-scale epidemiological study and anticoccidial efficacy tests revealed that these Togolese medicinal plants can be sustainable and cost-effective strategies for coccidiosis control. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/. © The Author(s) 202
PROSPECTS FOR THE USE OF MEDICINAL PLANTS EXTRACTS (Mallotus oppositifolius AND Kalanchoe crenata) AS ANTIMICROBIALS AGAINST SALMONELLOSIS IN POULTRY
No full textThis study is a contribution to the search for alternatives to combat antibiotic resistance in Salmonella strains in poultry farming. The objective of this work is to highlight the main phytochemical compounds of 2 Ivorian medicinal plants (Mallotus oppositifolius and Kalanchoe crenata) and to evaluate their acute oral toxicity with a view to their use in the poultry sector, to fight against certain avian pathologies, including Salmonellosis. The phytochemical compounds of the extracts of the plants used in this study were highlighted by colouring and precipitation methods. Acute oral toxicity was adapted to broilers according to the guideline OECD 425, 2008. The phytochemical screening carried out showed that aqueous extract of Mallotus oppositifolius possesses polyphenols and catechical tannins. The ethanolic extract also has anthraquinones, saponosides, sterols, and terpenes. The ethanolic extract of Kalanchoe crenata only owns polyphenols and catechical tannins. At the end of the acute oral toxicity study, no mortality was observed in all batches of experimentation and the biochemical analysis of the subject’s blood showed creatinine values ≤ 10 mg/L.; aspartate aminotransferase (ASAT) ≤ 275 IU/L; alanine aminotransferase (ALT) ≤ 50 IU/L, urea=0.01g/L, CRP≤6 mg/L; and blood sugar was between 2 and 5 g/L in subjects from different batches. Ultimately, the aqueous and ethanolic extracts of Mallotus oppositifolius and the ethanolic extract of Kalanchoe crenata can be used as an antibacterial in broiler farming. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/. © The Author(s) 202
CHITOSAN OLIGOSACCHARIDES AS DIETARY ANTIOXIDANTS IN NUTRITION OF BROILER CHICKENS: A REVIEW
No full textChitosan oligosaccharides (COS) have attracted considerable attention in poultry research due to their diverse biological activities and possible effects on the welfare and productivity of broiler chickens. A thorough examination of many studies indicates that the influence of COS on indices such as antioxidative functions, growth performance, immunological responses, and metabolic implications in broilers is significant. For example, specific dosage levels of COS have significantly enhanced antioxidant activity, regulation of cholesterol levels, and improved growth performance. The research findings have provided evidence for COS's antioxidative and anti-inflammatory properties and its capacity to mitigate the effects caused by stress. Nevertheless, the effectiveness of reactions might be influenced by the dosage and may demonstrate variances. Broiler chickens are suggested to consume dietary COS levels between 350 mg/kg and 500 mg/kg feed to obtain antioxidant and immunological advantages. Nevertheless, the most favourable results regarding growth and the ability to absorb nutrients are typically found when the intake ranges from 0.5 g/kg to 1.0 g/kg feed. For yellow-feather broilers experiencing heat stress, it is advisable to administer a dosage of 200 mg/kg feed of COS. However, it is essential to closely observe dosages exceeding 2.5 g/kg since they may significantly impair growth performance. The diverse research on using COS in broiler management has provided valuable insights into its intricate nature. This review has highlighted the potential benefits of COS in enhancing chicken health and nutrition. However, it has also underscored the need for additional research to optimize its effectiveness entirely in broiler performance. It can be concluded that dietary COS in broiler chickens in doses ranging from 200 mg to 1000 mg/kg feed has a positive effect on growth performance, antioxidative properties, regulation of lipid metabolism, ability to mitigate stress, impact on meat quality, and carcass traits, but exceeding 2.5 g/kg feed may significantly impair in growth performance in the broiler chickens strain Cobb 500. © The Author(s) 202
THE HEALTH AND ECONOMIC DIMENSIONS OF HONEY PRODUCTION IN IMO STATE, NIGERIA
No full textA study was conducted on honey production in Imo state of Nigeria, with a focus on the health and economic dimensions of the industry. The research was carried out using a multi-stage sampling procedure, and a sample size of 80 honey-producer respondents was selected. Data was collected through a well-structured questionnaire and analyzed using descriptive and inferential statistics. The study found that honey producers in the area had a mean age of 51 years, 11 years of education, 21 years of farming/beekeeping experience, and a household size of 6 persons. The average annual household income was €709.10, with a farm size/number of hives kept of 72 hives per farmer and a quantity of honey produced per annum of 145 litres. The cost and returns analysis showed that the cost of production of honey per litre and profit per litre were €0.40 and €2.40, respectively. The study also determined the nutritional uses and health benefits of honey (e.g. healing wounds, treating ulcers, controlling sore throats and colds, boosting immunity, and as an antibacterial agent). Several factors, including uncontrolled bush burning, bee forage shortage, deforestation, theft of beehives, colony absconding, and poor agricultural practices which strongly constrain honey production has been observed. It is concluded that honey production is a very profitable venture with numerous uses and health benefits and venturing youths into honey production as a source of livelihood should be encouraged, and extension education should be tailored to technologies in beekeeping and the identification of genuine honey to minimize the success of adulteration, among others. © The Author(s) 202
IDENTIFICATION OF OF SOME GENETIC MARKERS AS PRODUCTIVE AND REPRODUCTIVE TRAITS IN UKRAINIAN DAIRY CATTLE BREEDING
No full textSelection based on DNA markers is a breeding technique grounded in the genomic selection value of animals. The aim of the research is to study the genotypic profile of cows of different breeds in Ukrainian breeding in relation to the genes LEP, CSN3, TG5, BLG, and Pit-1 and to identify the probability of using them as markers for reproductive traits in cows. During the study, the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method was used to analyze the genes and determine their polymorphic characteristics. The obtained data indicated that the same gene variants have varying effects in the studied breeds due to their diverse influences on the genomic background. Specifically, the homozygous state of leptin genes (LEPCC) and pituitary-specific transcription factor (PIT-1BB) was observed to impact the reproductive characteristics of cows in the evaluated breeds. Meanwhile, for the genes casein (CSN3AB), thyroglobulin (TG5CT), and beta-lactoglobulin (BLGAВ), the heterozygous state of alleles was found to influence the key reproductive traits of dairy cattle of Holstein origin. The polymorphism of the genes CSN3, βLG, TG, PIT-1, and LEP indicated the presence of genetic potential for the reproductive function of cows and can be utilized as molecular markers in selective breeding, providing significant progress in improving not only the traits of dairy cattle productivity but also reproductive function. Therefore, in the implementation of selective breeding work, it is advisable to consider genotyping for the genes CSN3, βLG, TG, PIT-1, and LEP as an additional criterion for the selection of animals to enhance both their milk and reproductive characteristics. © (2024) Scienceline Publication
Carcass Characteristics and Blood Biochemical Parameters of Cobb-500 and Hubbard Chicken Strains Fed on Commercial and Farm-Formulated Diets
No full textThe limits of commercial diets, their quality, and their rising costs are some of the major challenges to broiler production in Ethiopia. The purpose of this investigation was to evaluate carcass yield characteristics and blood biochemical parameters of Cobb-500 and Hubbard chicken strains fed on farm-formulated diets (T1) and three different commercial diets (T2, T3, and T4). A total of 384 mixed-sex day-old chicks (192 per strain) were randomly assigned to four dietary treatments with four replicates, each consisting of 12 broilers. The experiment was set up as a 2 × 4 factorial design, providing each strain with four diets in a completely randomized design. After 42 days of the experiment, one male and one female of each strain from each pen (eight birds per treatment) were slaughtered for carcass yield and hematological analysis. Although diets had a significant impact on live body weight, feed conversion ratio, and feed consumption among the study treatments, they had no significant effect on the mortality rate of the broilers as a whole. There was a significant effect of strains on the weight of eviscerate, dress, thigh, drumstick, breast, neck, back, and eviscerate yield percentage, with Cobb 500 showing higher values than Hubbard broilers. The farm-formulated diet (T1) significantly increased the weight of non-edible offal compared to the commercial diets, except for the weight of crops and lungs, which were similar to those in commercial diet group T4. The Hubbard strain showed a higher least square mean for packed cell volume than the Cobb-500 strain. Sex was found to have no significant impact on the hematological parameters. The farm-formulated diet (T1) also resulted in a higher marginal return rate than that of the commercial diet (T3) in the Cobb-500 strain. These findings suggest that locally sourced farm-formulated diets could be a viable alternative to commercial diets for broiler chickens in the study area. © The Author(s) 202