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PHYSICOCHEMICAL COMPOSITION OF CRIOLLO AND CRIOLLO X SAANEN GOAT MILK ACCORDING TO AGE AND PARITY IN THE CENTRAL HIGHLANDS OF PERU
No full textPresent study aimed to analyze the physicochemical composition of goat milk based on the type of animal, age, and parity in Chupuro, Junin region in the country of Peru. A total of 24 goats were randomly selected, and divided into two groups: 12 native goats and 12 native goats crossed with Saanen. These groups (G1, G2, and G3) included three age categories (1.8, 2.5, and 3.2 years) and three parity levels (first, second, and third parity). The animals were monitored and fed considering their conversion and nutritional requirements. Before the morning milking, 100 ml of milk was extracted in sterile bottles, followed by agitation for 3 to 5 minutes, and the samples were transported using conservation and cooling techniques in a thermal box with ice cubes. These samples were analyzed in the special laboratory. Measurements of pH, acidity, density, lactose, total solids, fat, and protein were conducted using milk analyzer. The findings for the Criollo breed revealed a pH of 6.35 ± 0.31, a lactose concentration of 4.35, total solids of 11.62 ± 1.31, protein content of 4.12 ± 0.35, and fat content of 3.40 ± 0.91. In comparison, the Criollo x Saanen crossbreed exhibited a pH of 6.43 ± 0.13, a lactose concentration of 4.45, total solids of 12.63 ± 0.92, protein content of 4.26 ± 0.28, and fat content of 3.95 ± 0.69. The results indicated that there were no significant differences in the types of milk from native goats and native goats crossed with Saanen. However, significant differences (P<0.05) were observed in density, lactose, total solids, fat, and protein between groups of different ages and parity levels. Crossbreeding with the Saanen breed is well received in the region, as it serves to improve milk production, with favorable percentages of fat, protein, lactose, and total solids. © The Author(s) 202
USING TANNERY WASTES IN POULTRY FEED: A MATTER OF CONCERN FOR SAFE POULTRY PRODUCTION IN BANGLADESH
No full textNowadays tannery waste is a matter of concern because if it used as livestock feed, it could cause health hazards to humans. Therefore, this study was conducted to know the generation rates, utilization, disposal method of tannery solid wastes (TSWs), and inclusion level of it into the poultry feed. Moreover, this study determined the physical and chemical parameters of interest including moisture content, crude protein (CP) levels, and the presence of heavy metals such as chromium (Cr) and lead (Pb) in the poultry feeds that were sold in the studied area. For these purposes, a field survey was conducted with twenty tannery industries. Broiler feed samples were procured from multiple farmers situated in the Savar area of Dhaka. The feed source was classified into two categories, namely registered and unregistered feed mills. According to the study, wet blue trimmings was generated in 3.84 that was used as an ingredient of poultry feed. In addition, 55 of tanneries landfilled their waste, while 30 of them sold it for poultry feed. The utilization of TSWs in poultry feed production in this country was limited to a maximum of 1.314 of the total annual production. Besides, the CP was determined in the range of 24.24 - 13.32 and 18.15 - 11.01 for broiler starters and growers, respectively, where lower CP content was found only in unregistered feed mills. Trace amounts of Cr and Pb were identified in each of the feed samples at very low concentrations. In conclusion, it can be stated that the percentage of tannery solid wastes mixed poultry feed was generated in negligible amounts and the registered companies’ feed was found normal in all aspects of the quality tested in the study. © The Author(s) 202
EFFECTS OF PROBIOTIC Enterococcus faecium AND RAW, SPROUTED AND FERMENTED PEARL MILLET BASED DIETS ON PERFORMANCES, CARCASS TRAITS, HEMATOLOGICAL AND BIOCHEMICAL INDICES OF BROILER CHICKENS
No full textProbiotics, recognized as a safe substitute for antibiotics in the animal industry, have been acknowledged for their growth-enhancing properties. This study assessed the impact of Enterococcus faecium strain NCIMB 11181 and diets incorporating Raw, Sprouted, and Fermented pearl millet on the performance, carcass traits, organ weights, and blood parameters of broiler chickens. In a randomized design, 120 one-day-old Arbor Acre broiler chickens were assigned to five groups: 1) No supplement, negative control (N-con); 2) Control + antibiotics, positive control (P-con); 3) Raw pearl millet + probiotics in drinking water (RPM + PRO); 4) Sprouted pearl millet + probiotics in drinking water (SPM+PRO); 5) Fermented pearl millet + probiotics in drinking water (FPM + PRO). Probiotic supplementation did not significantly impact body weight gain (BWG) but influenced feed intake (FI) (P0.05) was observed on hematological indices. Overall, pearl millet diets supplemented with probiotics demonstrated no adverse effects on the health status of broiler chickens, suggesting their potential as viable alternatives to antibiotics. © The Author(s) 202
Efficacy and Safety of an Inactivated Novel Variant Infectious Bursal Disease Virus in Broiler Chickens
No full textThe infectious bursal disease virus (IBDV) is severe and highly contagious, causing high mortality and immunosuppression in chickens worldwide. A new novel variant, IBDV (nVarIBDV), has recently emerged in Asian countries, including Malaysia, highlighting the need to develop a new vaccine against this strain due to the inadequacy of existing commercial vaccines in protecting chickens from nVarIBDV infection. Therefore, the current study aimed to evaluate the efficacy and safety of inactivated nVarIBDV as a potential vaccine candidate in broiler chickens. A total of 65 one-day-old Arbo Acres broiler chickens were randomly divided into three groups (five animals in each group with four replications) before the challenge, namely A, B, and C. Groups A and B were immunized subcutaneously at day old with inactivated nVarIBDV (107 EID50/0.2 ml), and Group B was boosted at day 14. Group C was an unimmunized control. The experimental animals were divided into three subgroups and were challenged with pathogenic nVarIBDV (105 EID50/1.0ml) on day 28 post-inoculation through ocular and oral routes. The challenge sub-groups were named ACH, BCH, and CCH, respectively. The live body weight, bursa weight, and blood samples of the chickens were recorded. Gross lesions were examined, and samples of the bursa of Fabricius were collected from all the groups for histological evaluation. All the chickens appeared healthy and normal throughout the trial. Body weight increased in all groups without significant differences. The bursa weight and the bursa-to-body weight ratio of the booster group (Group B) were significantly higher than the non-booster and control groups. Gross lesions were not observed in the investigated groups. The challenged control group had higher bursa lesion scoring than the vaccinated groups. The IBDV antibody titer of challenged chickens in ACH, BCH, and CCH groups was higher than those of unchallenged groups A, B, and C at 35 days post-inoculation. The IBDV antibody titer of challenged chickens in group B was higher than challenged chickens in groups A and C (ACH and CCH). In conclusion, the inactivated nVarIBDV demonstrated safety and efficacy, with the booster Group (B) showing elevated humoral immune responses compared to the non-booster group. © The Author(s) 202
How to Design a Biomonitoring Study – A Practical Guide for Veterinary Professionals under a One Health Approach
No full textCurrently, veterinarians can see their daily practice and medical tasks as constant opportunities for passive surveillance of One Health threats, such as infectious zoonotic diseases and chemical pollution effects on living beings. The present study aimed to provide a practical guide to designing a biomonitoring study during veterinary clinical practice without time-consuming procedures or significant costs. The constant access to several species’ specimens provides the necessary samples to perform a biomonitoring study of environmental pollutants at the regional or national level. Generally, most health professionals know what to do (or where to find information) to report a disease outbreak. However, a summarized background to perform a biomonitoring study of a chemical hazard is missing. The authors of the current study provided a flow chart with the main steps to conduct a biomonitoring study in different fields of veterinary medicine. Thus, a biomonitoring study might give veterinarians (as other health professionals) a positive contribution to the clinical cases’ resolution, while improving the general knowledge about the impact of environmental contamination on animals and human health. © The Author(s) 202
A Holistic Approach to Bovine Brucellosis: Serological Tests, Knowledge, Attitudes, and Practices Evaluation, and Risk Factor Identification on Dairy Farms of Ethiopia
No full textBrucellosis, a contagious bacterial disease affecting animals globally presents a substantial zoonotic risk that is frequently underestimated, hinders animal trade, and endangers livestock and human health. The present study was conducted from November 2023 to June 2024 in Central and North Gondar Zone, Ethiopia. The current cross-sectional study aimed to evaluate the prevalence of brucellosis and to explore the related knowledge, attitudes, and practices within the specified region. A total of 384 serum samples were collected via random sampling from 20 dairy farms located in Ethiopia. Both local and cross-breed samples screened using the Rose Bengal Plate test and confirmed through an enzyme-linked immunosorbent assay. The seroprevalence of bovine brucellosis for both tests was 17.71 overall, with 9.62 for Central Gondar and 27.27 for North Gondar. Extensive farms exhibited notably higher odds of brucellosis compared to intensive farms, with unadjusted crude odds ratios of 3.01 and adjusted odds ratios of 2.37, respectively. Medium-sized herds also demonstrated increased odds in the multivariate analysis compared with small herds. Young respondents displayed the highest awareness levels, followed by adults and older individuals, with statistically significant differences observed across all categories. Regarding a semi-structured survey from 150 farmers on the association between sociodemographic data and knowledge, females exhibited higher awareness levels, with 117 (80.14) responding positively. Young respondents showed a higher positive response rate of 58 compared to adults (52.7) and the elderly (36.54). In conclusion, these results emphasize the need for comprehensive strategies to address the factors influencing bovine brucellosis prevalence and respondent awareness. © The Author(s) 202
Effects of Butylated Hydroxytoluene and Sorbitol as Diluent Components on Structural and Surface Ultrastructural Changes of Gaga Chicken Sperm During Cryopreservation
No full textThe Gaga chicken is an indigenous Indonesian breed that is important to preserve using semen cryopreservation technology. The study was conducted to determine the effect of adding sorbitol and butylated hydroxytoluene (BHT) in the diluent on the structural and surface ultrastructure of cryopreserved Gaga chicken sperm during cryopreservation/frozen storage. The study aimed to assess how adding sorbitol and butylated hydroxytoluene (BHT) to the diluent affects the structure and surface ultrastructure of cryopreserved Gaga chicken sperm. A completely randomized design was employed with four treatments and 10 replications including egg yolk-lactate ringer diluent (EYLR) as the control group, EYLR diluent with 3 mM BHT, EYLR diluent with 2 sorbitol, and EYLR diluent with both 3 mM BHT and 2 sorbitol. Semen was collected using a massage technique from 4 male chickens aged approximately 10 months, pooled semen was diluted, packaged in 0.25 mL straws, equilibrated for 2 hours at 5 °C, pre-freeze for 10 minutes, frozen for 24 hours, and thawed for 30 seconds at 37 °C. The parameters evaluated were sperm plasma membrane integrity, acrosome integrity, DNA damage, mitochondrial functionality, and surface ultrastructure. The results showed that the treatment had a significant effect on plasma membrane integrity and post-thawing mitochondrial functionality compared to the control, but no effect was observed on acrosome integrity or DNA damage. The results showed that the combination treatment of BHT with sorbitol had a significant effect on plasma membrane integrity and post-thawing mitochondrial function, but did not affect acrosome integrity or DNA damage when compared to the control group. Ultrastructural observations indicated that cryopreservation caused damage to the head, middle, and tail of the sperm in the control groups. However, these changes were prevented by the diluent containing a combination of BHT and sorbitol. The addition of both components (BHT 3 mM + sorbitol 2) effectively maintained plasma membrane integrity, mitochondrial functionality, and surface ultrastructure of Gaga chicken sperm during cryopreservation. © The Author(s) 202
Pathogenic and Antibiotic-Resistance Genes of Pasteurella multocida Isolated from Goats in the Mekong Delta, Vietnam
No full textPasteurella multocida (P. multocida) is one of the predominant pathogens that mostly cause respiratory diseases in domestic animals, such as goats. To determine P. multocida serotypes and the prevalence of pathogenic and antibiotic-resistance genes the PCR method was used. A total of 143 isolated P. multocida strains were collected from 289 healthy hybrid Boer-Saanen goats’ nasal samples in the Mekong Delta, Vietnam, from March to June 2023. A total of 143 P. multocida strains, serotype B accounted for the highest proportion (51.05), followed by serotype A (14.69), and the lowest was serotype E (0.70) while (39.86) of strains could not be determined serotypes. Among the six virulence genes surveyed, the sodA gene (56.64) had the highest presence, while the ompH gene (4.20) had the lowest presence. Pathogenic genes were present mainly in serotypes A and B; tbpA was frequently detected in serotype A (66.67), and sodA was commonly detected in serotype B (56.16). There were 14 virulence gene combinations in 59/109 (54.13) serotyped P. multocida strains, and the pattern of sodA + toxA + tbpA was prevalent at the highest rate (12.84). Moreover, among the eight investigated antibiotic resistance genes, the sulII gene had the highest presence rate (74.13), compared to the tetA gene with the lowest presence rate (13.29). Gene sulII was mainly detected on strains belonging to serotypes A (80.95), B (83.56), and F (77.78). A total of (77.98) of serotyped P. multocida strains indicated multi-harbor from two to six antibiotic-resistance genes, and the most common pattern was aadB + sulII (10.09). The prevalence of five pathogenic P. multocida serotypes harboring diverse antibiotic-resistance genes isolated from nasal samples could be a critical issue in treating and preventing the respiratory diseases caused by P. multocida in goats in the Mekong Delta. © The Author(s) 202
The Effects of Weather Conditions on Hematological and Biochemical Parameters in Dogs: A Field Study
No full textAlthough animals have adaptation abilities to different environmental conditions, various physiological changes may occur. The present research aimed to evaluate the effects of severe winter conditions on hematological and biochemical parameters in dogs kept outside all year. The research was carried out in the province of Kars, which is known for its severe cold conditions in Türkiye. Vital signs, hematological, and biochemical parameters of 12 adult dogs aged 1–8 years old (mixed breed, 8 males and 4 females) included in the study were compared in winter and summer seasons. The results indicated a significant effect of the winter season on the body temperature, respiration, and pulse rate of the dogs. In addition, it was observed that some hematological, including White blood cell (WBC), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) Hemoglobin (HB), and biochemical parameters (urea, TP, albumin, cholesterol, glucose, creatinine) of dogs differed in winter from those in summer. It should be considered that the vital values of dogs living outdoors in intense winter differed from those in mild winter or summer. It is also concluded that veterinarians should consider these differences in routine clinical examinations of these animals. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/. © The Author(s) 202
Effects of Different Dosages and Methods of Saponin Preparation from Mucuna pruriens Leaves on In Vitro Feed Digestibility
No full textThe Mucuna pruriens is commonly used in traditional medicine for anti-inflammatory, antibacterial, neuroprotector, antidiabetic, and anti-cancer purposes. The bioactive compounds, such as flavonoid, tannin, and saponin, could improve feed digestion efficiency in ruminants’ rumen. The current study aimed to evaluate the effects of different dosages and the two methods of saponin preparation from Mucuna pruriens leaves on in vitro feed digestibility parameters. A randomized block design with nested arrangements (2×5×3) was used in this study. Two methods of obtaining saponins from Mucuna pruriens leaves, including meal (MPLM) and extract (MPLE) of Mucuna pruriens leaves, were compared. The nested treatments of the preparation methods were the dosages of the saponin as feed additives in feed samples, involving 0, 0.025, 0.050, 0.075, and 0.10. There were 15 samples in each group (five-level dosage and three repetitions). The feed contained 40 forage and 60 concentrate. The obtained results indicated that saponin preparation from Mucuna pruriens leaves (MPLM and MPLE) significantly affected dry matter, organic matter, and crude fiber rumen degradability (r-DMD, r-OMD, r-CFD, respectively), as well as NH3, volatile fatty acid, propionate, butyrate concentrations, acetate-to-propionate (A/P) ratio, acetate, and propionate percentage. However, there was no significant impact on protozoa population, acetate concentration, butyrate percentage, in vitro dry matter digestibility (IVDMD), and in vitro organic matter digestibility (IVOMD). The MPLM saponin revealed significantly higher values on digestibility parameters except for protozoa, A/P ratio, and acetate percentage. The MPLM saponin dosage of 0.05 showed the highest values for r-DMD (56.48), r-OMD (56.51), and r-CFD (54.64), total Volatile fatty acid (77.71 mM), propionate (21.57 mM), propionate percentage (27.76), IVDMD (65.95), and IVOMD (65.86), but lowest in A/P ratio (2.04). In conclusion, the findings of the present study suggest that the MPLM saponin at a dosage of 0.05 holds promising potential for enhancing the fermentation profile in ruminants. © The Author(s) 2024. All Rights Reserved