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A Novel Application for Multiple Form Protein Separation by the Conjugation of Disc Electrophoresis and Isoelectric Focusing: Re-evaluating Cylindrical Disc-PAGE Tube System for Isoelectric Focusing and Potential Outcomes for Future Usage of this Approach
No full textAbstractAim: There are various forms of isoelectric focusing. But involving a single glass column and stabilized by the presence of a sucrose gradient, they are preferably used and are the most common pre-application step in 2D electrophoresis. The cylindrical disc-PAGE tube system is possibly not evaluated for isoelectric purposes. The goal here was to try a modified application for the separation of isoform proteins via the disc gel system. And to establish isoform separation and evaluate the implications of this approach for future use.Methods: Essentially, basic features of disc and isoelectric focusing were integrated. Although conventional disc electrophoresis requires three-layers of gel, but here only one form of gel layer was configured. As a second attempt, the homogenate and ampholite, were deliberately included in the unpolymerised gel medium as if they were gel preparation factors. Then, the separation of the isoform proteins, which were thought to be homogenously distributed in the presence of ampholite, was achieved.Results: The observation of isoforms of proteins revealed the presence of a distinct pattern of protein bands in their respective isoionic areas. From an analytical perspective, this application exhibited very clear band patterns. The results obtained regarding the isoforms of hemoglobin proteins were looking as informative and descriptive.Conclusions: The band pattern observed in the present approach clearly showed multiple isoform characters and simply demonstrated the differences of this novel application. It can be used to analysis and separate multiple of proteins in the first dimension of conventional two-dimensional electrophoresis.Keywords:IEF, IPG-Dalt, ampholite, isoform proteins, hemoglobins multiplicity, disc-PAGE System</p
Pursuit of entertainment or self-expression? Research on adventure tourism
No full textThis study explores the behavioral patterns of tourists in adventure tourism, focusing on the roles of push factors, self-image congruity, word-of-mouth (WOM), and intentions to revisit while considering the variations attributed to the rafting experience (first-time vs. repeated) and gender. Employing multi-group structural equation modeling to analyze 296 collected questionnaires, significant interrelations among these variables were revealed. Notably, joy emerged as a crucial sub-factor within push factors, particularly influencing inexperienced tourists’ intentions to revisit, whereas self-image congruity predominantly impacted experienced tourists. The study further found gender-specific differences, with males showing higher explained variance rates in self-image congruity and WOM. Experienced and male tourists gravitated more towards experiencing nature, whereas joy was more influential among inexperienced and female tourists. This research contributes to adventure tourism literature by elucidating the effect of push factors on self-image congruity and delineating the nuanced variations across different tourist experiences and genders
Technological Applications in Pharmaceutical Sciences
No full textOur book aims to bridge the gap between foundational research and the future of pharmaceutical innovation. The chapters curated here open new horizons across several high-impact domains. From the biochemical sophistication of Organ-on-a-Chip models described by Burcin Gungor to the precision of nanoparticle-mediated oncology addressed by Sumeyye Idil Celikkaya, the diversity of these topics reflects the complexity of modern drug discovery. The analytical core of the book is strengthened by the contributions of Bekir Caglar Celikkaya, and John A. Parkinson on NMR Spectroscopy, and Bekir Caglar Celikkaya on the modernization of DNA gel electrophoresis. Concluding with a forward-looking perspective, Pakize Canturk reviews the trajectory of Biotechnological Therapeutics.I would like to extend my deepest gratitude to all the contributing authors for their scholarly rigor and dedication. Their expertise has been instrumental in shaping this volume into a comprehensive resource that reflects the multi-faceted nature of modern pharmaceutical research.We respectfully present this work as a testament to the ongoing evolution and excitement within the pharmaceutical sciences.</div
Effects of plant powders on the technological and nutritional characteristics of gluten-free breads produced with black chickpea flour
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Al2O3-SU NANOAKIŞKANLI KARIŞIK TAŞINIM AKIMLARIYLA ÜST ÜSTE SIRALANMIŞ TAÇ DESENLİ ELEMAN DİZİSİNİN SOĞUTULMASI
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Ternary composite of polyacrylamide-chitosan-montmorillonite: Characterization and adsorptive features for thorium and BSA
No full textThe aim of this study was to prepare a hydrogel composite of chitosan and montmorillonite entrapped in PAAm (PAAmChMt) and to investigate its adsorption properties for Th4+ and bovine serum albumin (BSA). The structural characteristics of PAAmChMt were determined by a number of methods including FT-IR, XRD, SEM-EDX, TGA, BET-porosity analysis. The parameters defining adsorptive properties were consolidated by inspection of adsorption dependence on pH, concentration, kinetics, thermodynamics, and ionic strength. The structural evaluation tests affirmed the formation of the composite in a ternary combination. The isotherms acquired from the adsorption dependency on concentration of Th4+ and BSA were Type-I of IUPAC classification pointing out the high affinity of Th4+ and BSA to PAAmChMt. The concordance of experimentally obtained isotherms with the considered adsorption models was significant for both species. The magnitude of adsorption energy strengthened that the physicochemical nature of the sorption of Th4+ or BSA was Coulomb electrostatic interactions. The enthalpy and entropy changes were ΔH>0 and ΔS>0 for Th4+ and BSA sorption on PAAmChMt. This indicated that the Gibbs free enthalpy change was ΔG<0, i.e. the adsorption process was spontaneous. The coherence of the sorption kinetics with the first-order model was established by the Langmuir kinetics of Liu and Shen, after finding its interesting coherence with both pseudo-first/second-order models
