San Raffaele Open Research Data Repository
Not a member yet
162 research outputs found
Sort by
Choice of Template Delivery Mitigates the Genotoxic Risk and Adverse Impact of Editing in Human Hematopoietic Stem Cells.
No full textRaw data from the main figures of the article by Ferrari, Jacob, Cesana et al, Cell Stem Cell (https://doi.org/10.1016/j.stem.2022.09.001)
InsB9-23 Gene Transfer To Hepatocytes-Based Combined Therapy Abrogates Recurrence Type-1 Diabetes After Islet Transplantation.
No full textdoi: 10.2337/db19-1249
tabular data related to figure reported (.csv)
descriptive document of tabular data (.rtf
Antihypertensive treatment guided by genetics: PEARL-HT, the randomized proof-of-concept trial comparing rostafuroxin with losartan
No full textWe compared a standard antihypertensive losartan treatment with a pharmacogenomics-guided rostafuroxin treatment in never treated
Caucasian and Chinese patients with primary hypertension. Rostafuroxin is a digitoxigenin derivative that selectively
disrupts the binding to the cSrc-SH2 domain of mutant α-adducin and of the ouabain-activated Na-K pump at 10–11 M. Of 902
patients screened, 172 were enrolled in Italy and 107 in Taiwan. After stratification for country and genetic background,
patients were randomized to rostafuroxin or losartan, being the difference in the fall in office systolic blood pressure (OSBP)
after 2-month treatment the primary endpoint. Three pharmacogenomic profiles (P) were examined, considering: P1, adding to
the gene variants included in the subsequent P2, the variants detected by post-hoc analysis of a previous trial; P2, variants of
genes encoding enzymes for endogenous ouabain (EO) synthesis (LSS and HSD3B1), EO transport (MDR1/ABCB1), adducin
(ADD1 and ADD3); P3, variants of the LSS gene only. In Caucasians, the group differences (rostafuroxin 50 μg minus losartan
50 mg in OSBP mmHg) were significant both in P2 adjusted for genetic heterogeneity (P2a) and P3 LSS rs2254524 AA [9.8
(0.6–19.0), P=0.038 and 13.4 (25.4–2.5), P =0.031, respectively]. In human H295R cells transfected with LSS A and LSS C
variants, the EO production was greater in the former (P=0.038); this difference was abolished by rostafuroxin at 10–11 M.
Chinese patients had a similar drop in OSBP to Caucasians with losartan but no change in OSBP with rostafuroxin.
DOI 10.1038/s41397-021-00214-
Suture-Mediated Percutaneous Patent Foramen Ovale Closure: A Systematic Transesophageal Echocardiography Study
No full textThe present study was conducted to evaluate the efficacy and safety of suture-mediated Percutaneous patent foramen ovale (PFO) closure by transoesophageal echocardiography (TEE), which has been shown to have higher sensitivity than TTE for detection of residual shunt and characterisation of device-related complications.
doi: 10.4244/EIJ-D-21-00242
Early diagnosis of bladder cancer by photoacoustic imaging of tumor-targeted gold nanorods
No full textDetection and removal of bladder cancer lesions at an early stage is crucial for preventing tumor relapse and progression. Photoacoustic imaging of targeted gold nanorods bound to tumor cells allowed for the detection of neoplastic lesions smaller than 0.5 mm that were undetectable by ultrasound imaging and bioluminescence. We here provide raw data that were used to generate information reported in the article with DOI: 10.1016/j.pacs.2022.100400
- Photoshop files can be opened with ImageJ, which is free
- Prism files can only be opened with Prism, and those who do not have it can use the demo version which is free for 30 days (and then transfer the data in excel)
- TIFF images that can be opened with any image program, including Image
Higher Interleukin 13 differentiates patients with a positive history of suicide attempts in major depressive disorder
No full textEvery 40 seconds one person dies from suicide worldwide, half of which are related to depression. Neuroinflammation has been reported in neuropsychiatricconditions, including major depressive disorder (MDD). Preliminary findings suggested a link between inflammation and suicidal behavior. Nevertheless,to the best of our knowledge, no previous study explored how a wide range of cytokines may differentiate lifetime suicidal behaviors (i.e. positive history of suicide attempts) in patients affected by MDD. In 28 healthy controls (HC), 28 MDD patients with a positive history of attempting suicide (SA), and 28 MDD patients with a negative history (nSA), we
determined concentrations of immune analytes using an ultra-sensitive Pro Human Cytokine Group 27-Plex Panel and Chemokine immunoassay panels (Bioplex). To deal with the 27 highly correlated cytokines we applied two different statistical approaches that reduce the dimensionality of data: Principal Component Analysis, exploring if suicidality may be related to a comprehensive derived inflammatory state, and elastic-net penalized regression that allows considering as predictor every single cytokine.
Biological data are not uploaded due to ethical restrictions.
https://doi.org/10.1016/j.jadr.2021.10025
Intrinsic factor autoantibodies by luminescent immuno-precipitation system in patients with corpus atrophic gastritis
No full textJ Transl Autoimmun . 2021 Nov 1;4:100131. doi: 10.1016/j.jtauto.2021.100131. eCollection 2021.
Intrinsic factor autoantibodies by luminescent immuno-precipitation system in patients with corpus atrophic gastritis
Ilaria Marzinotto et a
Data from The outcome of fluocinolone acetonide intravitreal implant is predicted by the response to dexamethasone implant in diabetic macular oedema.
No full textThe outcome of fluocinolone acetonide intravitreal implant is predicted by the response to dexamethasone implant in diabetic macular oedema.
DOI: 10.1038/s41433-020-01373-
Long-term consequences of COVID-19 on cognitive functioning up to 6 months after discharge: role of depression and impact on quality of life
No full textNeurologic and psychiatric symptoms have been reported in the months following the infection with COVID-19. A low-grade inflammation has been associated both with depression and cognitive symptoms, suggesting a link between these disorders. The aim of the study is to investigate cognitive functioning 6 months following hospital discharge for COVID-19, the impact of depression, and the consequences on quality of life. Ninety-two COVID-19 survivors evaluated at 1-month follow-up, 122 evaluated at 3 months and 98 evaluated at 6 months performed neuropsychological and psychiatric evaluations and were compared with a healthy comparison group (HC) of 165 subjects and 165 patients with major depression (MDD). Cognitive performances were adjusted for age, sex, and education. Seventy-nine percent of COVID-19 survivors at 1 month and 75% at 3- and 6-month follow-up showed cognitive impairment in at least one cognitive function. No significant difference in cognitive performances was observed between 1-, 3-, and 6-months follow-up. COVID-19 patients performed worse than HC but better than MDD in psychomotor coordination and speed of information processing. No difference between COVID-19 survivors and MDD was observed for verbal fluency, and executive functions, which were lower than in HC. Finally, COVID-19 survivors performed the same as HC in working memory and verbal memory. The factor that most affected cognitive performance was depressive psychopathology which, in turn, interact with cognitive functions in determining quality of life. Our results confirm that COVID-19 sequelae include signs of cognitive impairment which persist up to 6 months after hospital discharge and affect quality of life.
DOI: 10.1007/s00406-021-01346-
Native CGRP Neuropeptide and Its Stable Analogue SAX, But Not CGRP Peptide Fragments, Inhibit Mucosal HIV-1 Transmission
No full textDOI for this manuscript is: 10.3389/fimmu.2021.785072
Published in 2021 by Lucia Lopalco et al.
We tested the capacity of CGRP to inhibit HIV-1 infection in-vivo in humanized mice. We further compared the anti-HIV-1 activities of full-length native CGRP, its metabolically stable analogue SAX, and several CGRP peptide fragments containing its binding C-terminal and activating N-terminal regions. These agonists were evaluated for their capacity to inhibit LCs-mediated HIV-1 trans-infection in-vitro and mucosal HIV-1 transmission in human mucosal tissues ex-vivo.
A single CGRP intravaginal topical treatment of humanized mice, followed by HIV-1 vaginal challenge, transiently restricts the increase in HIV-1 plasma viral loads but maintains long-lasting higher CD4+ T-cell counts. Similarly to CGRP, SAX inhibits LCsmediated HIV-1 trans-infection in-vitro, but with lower potency. This inhibition is mediated via CGRP receptor activation, leading to increased expression of both langerin and STAT4 in LCs. In contrast, several N-terminal and N+C-terminal bivalent CGRP peptide fragments fail to increase langerin and STAT4, and accordingly lack anti-HIV-1 activities. Finally, like CGRP, treatment of human inner foreskin tissue explants with SAX, followed by polarized inoculation with cell-associated HIV-1, completely blocks formation of LC-T-cell conjugates and HIV-1 infection of T-cells. Raw data corresponding to: Kinetics of CD4 and FACS, ex vivo experiments SAX and LCT, FACS for langerin and WB analysis are attached here