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Altered Frequency and Phenotype of HLA-G-Expressing DC-10 in Type 1 Diabetes Patients at Onset and in Subjects at Risk to Develop the Disease.
No full textIn the manuscript "Altered Frequency and Phenotype of HLA-G-Expressing DC-10 in Type 1 Diabetes Patients at Onset and in Subjects at Risk to Develop the Disease" (doi: 10.3389/fimmu.2021.750162.), we reported, for the first time, the evaluation of a population of HLA-G-expressing tolerogenic dendritic cells in the peripheral blood of T1D patients and first-degree relatives demonstrating the impairment in the number and phenotype of DC-10 is associated with the risk to T1D.
The three dataset here loaded (Raw_data_1, 2, and 3) contain all the clinical and immunological data, supporting our conclusions, that have been used to build the manuscript.
An additional file (raw_data_description) contains the main guidelines to correctly associate the raw data with the corresponding figure/table in the manuscript
Factors associated with the risk of patients and healthcare workers to develop COVID-19 during digestive endoscopy in a high-incidence area
No full textThis study is a description of factors associated with patients and healthcare workers risk to develop COVID-19 during digestive endoscopy in a high-incidence area. Data reported that COVID-19 development after gastrointestinal (GI) endoscopy was uncommon both for patients and healthcare workers, probably mainly due to environmental factors. Only e combined EUS + ERCP resulted independent risk factors for probable or confirmed COVID-19 development after logistic regression analysis. Whether combined EUS + ERCP convey an increased risk deserves further investigation.
doi: 10.1016/j.gie.2020.07.01
Hepcidin levels predict COVID-19 severity and mortality in a cohort of hospitalized italian patients
No full textSeveral risk factors for SARS-COV2 infection are well established, including advanced age, male gender, strong inflammatory response, neutropenia and lymphopenia, and more recently hypoferremia. Hepcidin, the liver peptide hormone whose role is to regulate iron concentration in the blood stream, is at the crossroad between iron metabolism and inflammation, being positively regulated by iron itself and proinflammatory cytokines. However, its role as potential biomarker of COVID-19 severity has only partially been explored. In this paper, we analyzed plasma hepcidin levels in a well-characterized cohort of 111 Italian COVID-19 patients admitted at San Raffaele University Hospital, in Milan. In this cohort, all patients are hypoferremic and hepcidin levels do not correlate with iron, instead hepcidin expression is mainly driven by inflammation and correlates with markers of lung function. Although plasma iron levels remain equally low in all groups examined, both in hypoxemic and in non-surviving patients hepcidin levels are higher than in the corresponding control groups likely due to the strong inflammation. More interestingly, in critical patients in intensive care unit hepcidin is an independent predictor of mortality, at difference with parameters of inflammation. Overall our data add hepcidin as a marker of morbidity and outcome, especially in severely compromised patients.
doi: 10.1002/ajh.2602
miR-21 sustains CD28 signalling and low-affinity T-cell responses at the expense of self-tolerance
No full textDOI: 10.1002/cti2.1321
Objective
miR-21 is highly expressed in iNKT and activated T cells, but its T-cell autonomous functions are poorly defined. We sought to investigate the role of miR-21 in the development and functions of T and iNKT cells, representing adaptive and innate-like populations, respectively.
Methods
We studied mice with a conditional deletion of miR-21 in all mature T lymphocytes.
Results
Thymic and peripheral T and iNKT compartments were normal in miR-21 KO mice. Upon activation in vitro, miR-21 depletion reduced T-cell survival, TH17 polarization and, remarkably, T- and iNKT-cell ability to respond to a low-affinity antigens, without altering their response to high-affinity ones. Mechanistically, miR-21 sustained CD28-dependent costimulation pathways required to lower the T-cell activation threshold, inhibiting its repressors in a positive feedback circuit, in turn increasing T cell sensitivity to antigenic stimulation and survival. Upon immunization with the low-affinity self-epitope MOG35–55, miR-21 KO mice were indeed less susceptible than WT animals to the induction of EAE, whereas they mounted normal T-cell responses against high-affinity viral epitopes generated upon LCMV infection.
Conclusion
The induction of T cell responses to weak antigens (signal 1) depends on CD28 costimulation (signal 2). miR-21 sustains CD28 costimulation, decreasing the T-cell activation threshold and increasing their sensitivity to antigenic stimulation and survival, broadening the immune surveillance range. This occurs at the cost of unleashing autoimmunity, resulting from the recognition of weak self-antigens by autoreactive immune responses. Thus, miR-21 fine-tunes T-cell response and self/non-self discrimination
Serum IgG1 and IgG4 could contribute to partial control of viral rebound in chronically HIV-1 infected patients
No full textDOI for this work is: 10.1097/QAD.0000000000002944
Published in 2021 by Lucia Lopalco et al.
All the enrolled patients studied in this work were previously described in the APACHE study. The study was conducted on HIV chronically infected patients, with HIV-RNA 500 cells/μL and HIV-DNA 50 copies/mL). Raw data of clinical data of all described patients are reported in table I of the manuscript. Collection of sera and analysis of both binding antibodies (BAbs) and neutralizing antibodies (NAbs) was performed at three different time points: ATI, CVR and time of viral re-suppression after antiretroviral treatment (ART) resumption. IgG subclasses (IgG1, IgG2, IgG3 and IgG4) from the 4 patients with highest levels of neutralization were found to block viral infection (raw data are reported here in fig 2 Fig3 IgG HIV). All subjects had CVR after ATI at a median time of 21 days (14-56). After ART resumption, all the enrolled subjects achieved HIV-RNA<50 copies/mL in 42 days (21-98) (as shown in fig 1 of the manuscript). We observed a strong increase of either BAbs and NAbs titers from ATI to viral re-suppression in one patient, who showed the longest period of virus undetectability during ATI. In this patient, BAbs and NAbs specifically belonged to both IgG1 and IgG4 subclasses, directed to env antigen. Raw data (fig4 HIV) here reported reports the characterization of antibodies in this patients. Further details are shown in fig 4 of the manuscript
Diagnostic accuracy of EUS-FNA in the evaluation of pancreatic neuroendocrine neoplasms grading: Possible clinical impact of misclassification
No full textA dataset regarding patients undergone surgery and EUS-FNA for pancreatic neuroendocrine tumors. DOI: 10.4103/EUS-D-20-0026
Patients younger than 70 undergoing transcatheter aortic valve implantation: Procedural outcomes and mid-term survival
No full textIntroduction: Based on recent data, the indication for transcatheter aortic valve implantation (TAVI) is expanding to individuals at lower surgical risk, who are generally younger than subjects historically treated for severe aortic stenosis. Indeed, younger patients have traditionally been under-represented in current TAVI literature. The aim of the present study is to report about clinical features, procedural outcomes and mid-term outcomes of patients younger than 70 who underwent TAVI in a single high-volume center.
Materials and methods: Consecutive patients younger than 70 years of age who underwent TAVI for severe, symptomatic aortic stenosis between 2007 and 2019 at a single, tertiary referral center have been included in this retrospective study. Procedural and mid-term outcomes were analyzed, comparing 1st generation with 2nd generation devices.
Results: Between 2007 and 2019, 1740 TAVI procedures were performed in our center. Among these, one hundred twenty-nine (7.4%) patients were younger than 70 years at the time of the intervention and were included in the present analysis. Fifty-eight patients (45%) were implanted with a 1st generation prosthesis while seventy-one patients (55%) were implanted with a 2nd generation device. Reasons which lead to a transcatheter approach in this population were: previous CABG (27.9%); porcelain aorta (24%); severe left ventricular systolic dysfunction (21.7%); prior chest radiation (19.4%); severe lung disease (8.5%); hemodynamic instability (7.0%); advanced liver disease (4.6%) and active cancer (3.9%). Overall device success rate was 89%, with no differences among 1st and 2nd generation devices. Threeyears all-cause mortality was 34%, with no difference among the two groups. Low incidence of aortic-valve re-intervention was observed at mid-term follow-up (late valve re-intervention = 2.3%).
Conclusions: TAVI in young patient with appropriate indication for intervention is a safe procedure, associated with low rate of in hospital mortality and low rate of severe complications both with 1st and with 2nd generation devices. When considering long term durability, more data are needed; in our case series long-term follow up shows a good survival and also an extremely low rate of valve re-intervention.
doi: 10.1016/j.ijcha.2021.100817
Lessons from the Pandemic: Reshaping the Echocardiography Laboratory and Clues for Restarting
No full text10.1016/j.echo.2021.08.001
We report the reshaping process of a high-volume echocardiography laboratory in Lombardy, the hardest hit region in Italy, by analyzing echocardiographic examinations performed throughout the different phases of the Pandemic
Efficient and safe correction of hemophilia A by lentiviral vector-transduced BOECs in an implantable device
No full textData for the paper of Olgasi et al 2021 (PMID: 34853801 , PMCID: PMC8606349 , DOI: 10.1016/j.omtm.2021.10.015
Enhancement of doxorubicin anti-cancer activity by vascular targeting using IsoDGR/cytokine-coated nanogold
No full textRaw data and original images for the article entitled "Enhancement of doxorubicin anti-cancer activity by vascular targeting using IsoDGR/cytokine-coated nanogold" by Angelo Corti et al, J Nanobiotechnology. 2021 May 5;19(1):128. (DOI: 10.1186/s12951-021-00871-