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Dysregulation of ERAD and Stress Response Proteins by V493F FTO Over-expression A Proteomic Perspective
No full textBackground/Aim: Demethylase fat mass and obesity-related protein (FTO), which belongs to the AlkB homologous (ABH) family, is associated with various neurological diseases, cancer, and obesity. This protein, which contains many structurally and functionally different regions, contains a COOH-terminal domain whose function, unlike other ABH members, is not fully understood. This study aimed to investigate the effects of the exonic V493F mutation in this region of FTO on the soluble proteome.Materials and Methods: SH-SY5Y cells stably over-expressing wild-type (WT-FTO) or mutant FTO (V493F-FTO) proteins under the control of the Tet promoter were created and used. Comparative proteomic analysis using two-dimensional gel electrophoresis (2DE) identified over 500 protein spots, with 10 showing significant (≥2-fold) differential expression. These proteins were identified by MALDI-TOF/TOF mass spectrometry and validated by western blotting.Results: WT-FTO over-expression primarily affected proteins related to DNA replication and repair, including PCNA, whereas V493F-FTO over-expression altered the expression of stress response and endoplasmic reticulum-associated degradation (ERAD) pathway proteins, such as HSPA4, ARHGDIA, and VCP. Although the mutation did not alter the nuclear localization or predicted 3D structure of FTO, it distinctly modulated pathways associated with protein homeostasis and cellular stress.Conclusion: FTO participates in the regulation of the cellular stress response and the ubiquitin-dependent ERAD pathway, functions potentially independent of its demethylase activity. Importantly, dysregulation of these pathways has been implicated in cancer initiation, progression, and therapeutic resistance. Therefore, our findings provide new insights into how FTO mutations might influence oncogenic processes, highlighting FTO as a potential biomarker and therapeutic target in cancer biology.</div
Parameter-Efficient Hybrid Architectures for Multimodal Phishing Detection: Insights from the MTLP Dataset
No full textFrom jujube seeds to gas storage: Carbon-enhanced MCM-41 composites for CO2, CH4 and H2 adsorption
No full textIn this study, a series of novel carbon/MCM-41 composites were synthesized using various carbon sources, including jujube seed-derived activated carbon, commercial activated carbon, graphene, and multiwalled carbon nanotubes. The aim was to design advanced adsorbents with enhanced CO2, CH4, and H2 storage capabilities. The combination of microporous activated carbon with mesoporous MCM-41 resulted in hybrid structures offering improved gas diffusion and surface accessibility. Among all prepared composites, the JAC/MCM-41 material demonstrated superior adsorption performance across all three gases. The results highlight the synergistic effect of hierarchical pore structures and the potential of biomass-derived composites for multi-gas storage applications. Notably, the jujube seed-based composite achieved a CO2 adsorption capacity of 29.2 % at 273 K, exceeding that of the other tested materials
Aquaculture for food security and climate resilience in Somalia: Opportunities, challenges, adaptive pathways and policy recommendations
No full textSomalia faces chronic food insecurity driven by drought, climate shocks, conflict, and weak institutions. Aquaculture offers a climate-resilient option to improve food availability, support livelihoods, and diversify diets. This case study reviews peer-reviewed literature (2000–2025) and major institutional reports to assess aquaculture feasibility using the FAO Food Security Framework and Social-Ecological Systems resilience theory. Findings highlight opportunities for climate-smart systems, including recirculating aquaculture (RAS), integrated models, and hardy species such as Nile tilapia and African catfish. Key barriers include limited data, technical capacity, infrastructure, and cultural acceptance. Recent policy developments, such as the 2023 Law of Fisheries Management and Development, signal growing institutional commitment, but regulatory and value-chain gaps persist. Recommended pathways include small pilot projects, diaspora investment, cold-chain improvements, and a national aquaculture strategy. With targeted innovation and policy support, aquaculture could become a cornerstone of Somalia's climate-resilient food system
A rare primary mediastinal mesenchymal tumor diagnosed by endobronchial ultrasound (EBUS).
No full textGastronomide Kinoa İlaveli Tariflerin Geliştirilmesi ve Duyusal Analiz ile Karşılaştırılması
No full textA Small Indole Derivative Isolated From Caper (Capparis ovata) as an Inducer of P53-Mediated Apoptosis in Prostate Cancer: Comprehensive In Vitro and In Silico Studies.
No full textGlial Fibrillary Acidic Protein as a Biomarker in Acute Ischemic Stroke: Diagnostic Value and Prognostic Implications
No full textStroke is one of the leading causes of mortality and long-term disability worldwide, with ischemic stroke representing the most common subtype. Despite significant progress in neuroimaging and reperfusion therapies, rapid and accurate diagnosis remains a major challenge in clinical practice. Glial fibrillary acidic protein, an intermediate filament protein specific to astrocytes, has recently gained attention as a promising blood-based biomarker. Following astrocytic damage and disruption of the blood-brain barrier, glial fibrillary acidic protein is released into the extracellular space and enters the circulation. Although its levels rise rapidly in hemorrhagic stroke, the increase in ischemic stroke occurs more gradually, typically becoming measurable within the first 24 to 48 hours after the onset of symptoms. Clinical investigations have demonstrated that blood concentrations of glial fibrillary acidic protein are significantly higher in patients with ischemic stroke compared to healthy controls and that these elevations correlate with neurological severity, infarct volume, and functional outcomes. Thus, glial fibrillary acidic protein provides valuable prognostic insights and may support patient stratification. However, variability in cut-off values, differences in detection methods, and delayed kinetics remain important limitations. Future developments include the integration of glial fibrillary acidic protein into multimarker panels and the use of ultrasensitive point-of-care assays that may enable rapid decision-making in emergency settings. Overall, glial fibrillary acidic protein has the potential to serve as a complementary biomarker for diagnosis, prognosis, and monitoring in ischemic stroke, thereby contributing to improved patient care and individualized therapeutic strategies.</p
New Refinements of Hermite-Hadamard Inequalities for Quantum Integrals
No full textIn this study, we first introduce two functions including quantum integrals. Then, we prove some properties of these mappings such as convexity and monotony. Moreover, by using the newly defined mappings, we prove some refinements of the left hand side of Hermite-Hadamard inequality for left and right quantum integrals
Negative Viral Load Follow-Up in an Untreated HIV-2-Infected Patient: A Case Report
No full textHIV-2 is endemic in certain regions, particularly West Africa. A 64-year-old Ghanaian male, who had previously lived in Canada and had been residing in Türkiye for the past 10 years, was diagnosed with HIV-2 while living in Canada but had not received antiretroviral therapy. HIV-2 infection was confirmed by fourth-generation ELISA and Geenius™ assay, which demonstrated gp36 and gp140 positivity. The ‘in-house’ HIV-2 proviral DNA test was also positive. Over a three-month follow-up period, HIV-2 RNA remained negative, and CD4+ T lymphocyte levels showed no decline. To the best of our knowledge, this patient is the third reported HIV-2 case in Türkiye