Czasopisma Uniwersytetu Przyrodniczy w Lublinie
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The selected agrotechnical factors affecting the occurrence of black grass Alopecurus myosuroides in winter wheat crops in north-western Poland
Get PDFAlopecurus myosuroides of the family Poaceae is a highly competitive species for winter cereal crops, particularly for barley and wheat, less frequently for root vegetables or rapeseed. This study aimed to determine the predominant factor or group of factors affecting the cover of A. myosuroides in winter wheat field depending on the tillage systems, time, and amounts of herbicides used. The following variables differentiating the quantitative contribution of A. myosuroides in the analyzed fields were assumed: plough or no-plough tillage, date of biocidal application, and the total amount of herbicides (active substances) used. When spring-only herbicide was applied, the lowest A. myosuroides cover, not exceeding 25%, was observed in the margins of winter wheat fields. The coverage of A. myosuroides, reaching 25–100% of the winter wheat area, regardless of the tillage system, was found when the herbicides from the group of sulfonylurea derivatives (acetolactate synthase ALS inhibitors) were frequently used. Winter wheat infestation with A. myosuroides was significantly lower when ploughing was applied. The highest cover of A. myosuroides, reaching 50–100% of the area, was observed only in the central part of the analyzed winter wheat fields, following the application of herbicides in autumn and spring or in autumn only.Alopecurus myosuroides of the family Poaceae is a highly competitive species for winter cereal crops, particularly for barley and wheat, less frequently for root vegetables or rapeseed. This study aimed to determine the predominant factor or group of factors affecting the cover of A. myosuroides in winter wheat field depending on the tillage systems, time, and amounts of herbicides used. The following variables differentiating the quantitative contribution of A. myosuroides in the analyzed fields were assumed: plough or no-plough tillage, date of biocidal application, and the total amount of herbicides (active substances) used. When spring-only herbicide was applied, the lowest A. myosuroides cover, not exceeding 25%, was observed in the margins of winter wheat fields. The coverage of A. myosuroides, reaching 25–100% of the winter wheat area, regardless of the tillage system, was found when the herbicides from the group of sulfonylurea derivatives (acetolactate synthase ALS inhibitors) were frequently used. Winter wheat infestation with A. myosuroides was significantly lower when ploughing was applied. The highest cover of A. myosuroides, reaching 50–100% of the area, was observed only in the central part of the analyzed winter wheat fields, following the application of herbicides in autumn and spring or in autumn only
Produkcyjność soi uprawianej metodą proekologiczną i konwencjonalną w zależności od rozstawy rzędów
No full textSoybean (Glycine max (L.) Merr.) is a legume valued, among other things, for the high nutritional value of its seeds. It plays an important role in crop rotation, improving soil fertility and structure. Therefore, research aimed at determining the optimal agrotechnical conditions for obtaining high yields of this plant is crucial. The purpose of this research was to evaluate the yield of two soybean cultivars sown at varying row spacing under pro-ecological and conventional methods. A four-year field study proved that conventional cultivation had a more favorable effect than pro-ecological cultivation on seed yield, plant density after emergence, number of pods per plant and number and weight of seeds per plant. In pro-ecological cultivation, soybeans set pods significantly higher. The Merlin cultivar turned out to be a better yielder than Aldana (by 43.4%), in which the elements of crop and yield components were also more favorable. Significantly higher soybean seed yield was obtained at a row spacing of 22.5 cm than 35 cm.Soja (Glycine max (L.) Merr.) jest rośliną bobowatą cenioną m.in. za dużą wartość odżywczą nasion. Odgrywa ważną rolę w płodozmianie, poprawiając żyzność i strukturę gleby. Dlatego kluczowe znaczenie mają badania ukierunkowane na wyznaczenie optymalnych warunków agrotechnicznych dla uzyskania wysokich plonów tej rośliny. Celem przeprowadzonych badań była ocena plonowania dwóch odmian soi wysiewanych w zróżnicowanej rozstawie rzędów w warunkach uprawy metodą proekologiczną i konwencjonalną. Czteroletnie badania polowe udowodniły, że uprawa metodą konwencjonalną oddziaływała korzystniej niż proekologiczną na wielkość plonu nasion, obsadę roślin po wschodach, liczbę strąków z rośliny oraz liczbę i masę nasion z rośliny. W uprawie proekologicznej soja znacznie wyżej zawiązywała strąki. Lepiej plonującą okazała się odmiana Merlin niż Aldana (o 43,4%), u której korzystniej kształtowały się również elementy struktury plonu i łanu, z wyjątkiem masy 1000 nasion. Istotnie większy plon nasion soi uzyskano w rozstawie rzędów 22,5 cm niż 35 cm
Wielkość i jakość plonu niełupek słonecznika w zależności od typu odmiany
Get PDFThe field experiment was carried out in 2021 and 2022 on an individual farm in Szówsko, Podkarpackie Voivodeship. The aim of the study was to compare the size and quality of yields of selected sunflower cultivars: classic, resistant to tribenuron-methyl, resistant to imazamox. It was shown that the highest plants were developed by the MAS 83.SU cultivar and the lowest by the ES Agora cultivar. The largest number of achenes contained the heads of Elsasun IR and Jonasun IR cultivars, and the highest MTN was found in the cultivars ES Agora, MAS 83.SU, ES Boston SU, MAS 920.CP. Among the tested cultivars, Elsasun IR (3.36 t‧ha–1), MAS 83.SU (3.54 t‧ha–1) and Jonasun IR (3.52 t‧ha–1) had the highest yield, while Helesun SU (2.57 t‧ha–1) had the lowest yield. The high fat content in the achenes was characterized by the cultivars Sulfonom, Helesun SU, Florasun and Jonasun IR. In turn, the highest protein content was determined in the achenes of the Helesun SU, ES Boston SU and MAS 920.CP cultivars. The highest fat yield was given by the Jonasun IR, Elsasun IR and MAS 83.SU cultivars and protein yield by the ES Boston SU cultivar. Drought from May to August in 2022 limited plant growth and resulted in a lower yield and lower fat content and higher protein content.Doświadczenie polowe przeprowadzono w latach 2021 i 2022 w gospodarstwie indywidualnym w miejscowości Szówsko, województwo podkarpackie. Celem badań było porównanie wielkości i jakości plonów wybranych odmian słonecznika: klasyczne, odporne na tribenuron metylu, odporne na imazamoks. Wykazano, że najwyższe rośliny wykształciła odmiana MAS 83.SU, a najniższe odmiana ES Agora. Najwięcej niełupek zawierały koszyczki odmiany Elsasun IR i Jonasun IR, a najwyższą MTN odznaczyły się odmiany ES Agora, MAS 83.SU, ES Boston SU, MAS 920.CP. Spośród badanych odmian najwyżej plonowały Elsasun IR (3,36 t‧ha–1), MAS 83.SU (3,54 t‧ha–1) i Jonasun IR (3,52 t‧ha–1), natomiast najniżej plonowała odmiana Helesun SU (2,57 t‧ha–1). Wysoką zawartością tłuszczu w niełupkach odznaczyły się odmiany Sulfonor, Helesun SU, Florasun i Jonasun IR. Z kolei najwyższą zawartość białka oznaczono w niełupkach odmiany Helesun SU, ES Boston SU i MAS 920.CP. Najwyższy plon tłuszczu wydały odmiany Jonasun IR, Elsasun IR i MAS 83.SU, a plon białka odmiana ES Boston SU. Susza od maja do sierpnia w 2022 r. ograniczała wzrost roślin i skutkowała niższym plonem oraz mniejszą zawartością tłuszczu, a większą zawartością białka w niełupkach
Effect of increasing doses of zinc in combination with organic materials on the occurrence of entomopathogenic fungi in the soil
Get PDFThe aim of the study was to determine the effect of zinc application in different doses with organic fertilization on the genera composition and the number of CFU (colony-forming units) of entomopathogenic fungi (EPF) in the soil. The experiment was carried out in greenhouse condi-tions. Soil samples for testing were collected in the third year of the experiment (spring, autumn), where the research objects were I – dose of zinc: control – lack (0) and 200, 400 and 600 mg Zn kg–1 of soil; II – no fertilization – 0 (CO), spent mushroom substrate (SMS), chicken (ChM) and cattle manure (CM). Entomopathogenic fungi were isolated using the method of isolation on a selective medium. In the course of the research, EPF of the genus: Beauveria, Metarhizium, Cordyceps and Lecanicillium were determined. The conducted research showed that entomopatho-genic fungi of the Metarhizium genera formed the most CFU in spring. In the autumn there were three times less of them. Statistical analysis showed that the number of CFUs of the identified genus of fungi (on average) in soil samples significantly depended on the dose of zinc applied, organic fertilization and the genus of fungus, but only for Metarhizium spp.The aim of the study was to determine the effect of zinc application in different doses with organic fertilization on the genera composition and the number of CFU (colony-forming units) of entomopathogenic fungi (EPF) in the soil. The experiment was carried out in greenhouse condi-tions. Soil samples for testing were collected in the third year of the experiment (spring, autumn), where the research objects were I – dose of zinc: control – lack (0) and 200, 400 and 600 mg Zn kg–1 of soil; II – no fertilization – 0 (CO), spent mushroom substrate (SMS), chicken (ChM) and cattle manure (CM). Entomopathogenic fungi were isolated using the method of isolation on a selective medium. In the course of the research, EPF of the genus: Beauveria, Metarhizium, Cordyceps and Lecanicillium were determined. The conducted research showed that entomopatho-genic fungi of the Metarhizium genera formed the most CFU in spring. In the autumn there were three times less of them. Statistical analysis showed that the number of CFUs of the identified genus of fungi (on average) in soil samples significantly depended on the dose of zinc applied, organic fertilization and the genus of fungus, but only for Metarhizium spp
Multidimensional assessment of yield and quality of starchy potato cultivars
Get PDFTubers destined for starch production in potato industry need to meet certain commercial quality standards. The objective of the work was to conduct a multivariate assessment of yield potential and tuber quality of starchy potato cultivars. An analysis was performed of data describing potato cultivars listed in the National List of Potato Varieties (KRO) in Poland published by the Plant Breeding and Acclimatization Institute – National Research Institute at Radzików. Very early and early as well as medium late and late starchy potato cultivars were analysed in terms of 13 quantitative characteristic using principal component analysis and cluster analysis. It was demonstrated that, regardless of earliness group, the following traits had the greatest share in the total variation of starchy potato cultivars: tuber yield, starch content, starch yield and bioethanol yield. Cluster analysis allowed the division of very early and early cultivars into 3 groups. The cultivars Szyper, Zuzanna, Kuba and Jubilat, included in the first group, produced superior yields, the highest bioethanol yield and were the most resistant to potato blight. Medium late and late cultivars were divided into two groups. The first group (Pokusa, Gandawa, Rudawa, Hinga, Inwestor, Pasja, Pomorska and Ikar) gave poorer yields and lower bioethanol yields but were more resistant to potato blight in comparison with the second group (Sonda, Kuras, Jasia, Bzura, Skawa and Danuta). The multidimensional methods applied allowed a simultaneous assessment of starchy potato cultivars in terms of many characteristics, and grouping into clusters sharing similar traits.Tubers destined for starch production in potato industry need to meet certain commercial quality standards. The objective of the work was to conduct a multivariate assessment of yield potential and tuber quality of starchy potato cultivars. An analysis was performed of data describing potato cultivars listed in the National List of Potato Varieties (KRO) in Poland published by the Plant Breeding and Acclimatization Institute – National Research Institute at Radzików. Very early and early as well as medium late and late starchy potato cultivars were analysed in terms of 13 quantitative characteristic using principal component analysis and cluster analysis. It was demonstrated that, regardless of earliness group, the following traits had the greatest share in the total variation of starchy potato cultivars: tuber yield, starch content, starch yield and bioethanol yield. Cluster analysis allowed the division of very early and early cultivars into 3 groups. The cultivars Szyper, Zuzanna, Kuba and Jubilat, included in the first group, produced superior yields, the highest bioethanol yield and were the most resistant to potato blight. Medium late and late cultivars were divided into two groups. The first group (Pokusa, Gandawa, Rudawa, Hinga, Inwestor, Pasja, Pomorska and Ikar) gave poorer yields and lower bioethanol yields but were more resistant to potato blight in comparison with the second group (Sonda, Kuras, Jasia, Bzura, Skawa and Danuta). The multidimensional methods applied allowed a simultaneous assessment of starchy potato cultivars in terms of many characteristics, and grouping into clusters sharing similar traits
Wpływ zróżnicowanej rozstawy rzędów i ilości wysiewu na rozwój i plonowanie soi (Glycine max (L.) Merrill). Cz. I. Rozwój i cechy morfologiczne soi
Get PDFIn 2015–2017, in the proving grounds of the Institute of Agroecology and Plant Production of Wrocław University of Environmental and Life Sciences, field studies were carried out on the different row spacing and sowing amount on the development and yielding of soybean. The test included the assessment of the impact of the varied spacing of rows (15 cm, 30 cm) and the number of sown seeds (50, 75, 90) per unit area. In both of test, the “split-plot” method was used, four repetitions, with two variable factors. The length of the growing seasons of soybean was influenced by the variable thermal and humidity conditions in individual years of the research. In the studies, the factor of differentiated row spacing (15 cm, 30 cm) significantly determined: the number of pods, the number and mass of seeds per plant, the mass of 1000 seeds. The increasing number of sown soybean seeds from 50 to 90 pieces per 1 m2 resulted in a significant increase in the height of the first pod placement, while causing a gradual decrease in the number of 1st order branches, the number of pods per plant, the number and weight of seeds per plant and the weight of 1000 seeds.W latach 2015–2017 na polach doświadczalnych Instytutu Agroekologii i Produkcji Roślinnej Uniwersytetu Przyrodniczego we Wrocławiu przeprowadzone zostały badania dotyczące wpływu zróżnicowanej rozstawy rzędów i ilości wysiewu na rozwój i plonowanie soi. W badaniach polowych zastosowano metodę split-plot, w czterech powtórzeniach, z dwoma czynnikami: I czynnik – zróżnicowana rozstawa rzędów (15 cm, 30 cm), II czynnik – zróżnicowana liczba wysiewanych nasion (50, 75, 90 nasion na 1 m2). Długość okresów wegetacyjnych soi uprawnej była kształtowana pod wpływem zmiennych warunków wilgotnościowo-termicznych. W badaniach czynnik zróżnicowanej rozstawy rzędów (15 cm, 30 cm) istotnie determinował: liczbę strąków płodnych, liczbę i masę nasion z rośliny, masę 1000 nasion. Wzrastająca liczba wysiewanych nasion soi z 50 do 90 sztuk na 1 m2 powodowała istotny wzrost wysokości osadzenia pierwszego strąka, powodując jednocześnie stopniowe zmniejszanie liczby rozgałęzień pierwszego rzędu, liczby strąków na roślinie, liczby i masy nasion z rośliny oraz masy 1000 nasion
Analysis of the multi-annual effect of tillage systems and forecrops on texture and physical properties of soil
Get PDFThe research topic was the analysis of the effect of multi-annual application of varied tillage systems and forecrops on soil texture and physical properties. The field experiments were carried out in Lipnik at the Agricultural Experimental Station belonging to the West Pomeranian University of Technology in Szczecin. Two factors were compared in the course of the experiment: factor I – 3 tillage systems: ploughing (A), ploughless (B), direct sowing (C); factor 2 – forecrop: 1 – Faba bean, 2 – sugar beet. The effect of forecrop was stronger with respect to chemical composition of soil, whereas tillage system was found to have a stronger effect on the physicochemical properties of soil. Field water capacity of the soil in the experiment was 10%. The use of reduced tillage systems did not cause changes in soil moisture, in comparison with the conventional tillage system. With direct sowing, there was a significant increase in soil stability, as compared with ploughing (lower RDC values = 0.320 g 100 g-1). This relationship was found only in the deeper layer of soil (0.05-0.20 m). The effect of reduced tillage was manifested in an increase of the value of S(f) indicator and, consequently, structural degradation of soil. The use of Faba bean as forecrop was found to result in higher values of S(f). Direct sowing caused a significant increase in soil stability in comparison with ploughing. However, this relationship was identified only with respect to the deeper layer of soil.Tematem badań była analiza wpływu wieloletniego stosowania zróżnicowanych systemów uprawy gleby i przedplonów na uziarnienie i właściwości fizyczne gleby. Doświadczenia polowe przeprowadzono w Lipniku w Rolniczym Zakładzie Doświadczalnym należącym do Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie. W doświadczeniu porównano dwa czynniki: czynnik I – 3 systemy uprawy: orkowy (A), bezorkowy (B), siew bezpośredni (C); czynnik 2 – przedplon: 1 – bobik, 2 – burak cukrowy. Wpływ przedplonu był silniejszy na skład chemiczny gleby, natomiast system uprawy gleby miał większy wpływ na właściwości fizykochemiczne gleby. Polowa pojemność wodna gleby w doświadczeniu wynosiła 10%. Stosowanie systemów uprawy uproszczonej nie powodowało zmian wilgotności gleby w porównaniu z uprawą konwencjonalną. Przy siewie bezpośrednim nastąpił istotny wzrost stabilności gleby w porównaniu z orką (niższe wartości RDC = 0,320 g 100 g-1). Zależność tę stwierdzono jedynie w głębszej warstwie gleby (0,05-0,20 m). Efekt uprawy uproszczonej przejawiał się wzrostem wartości wskaźnika S(f), a w konsekwencji degradacją strukturalną gleby. Stwierdzono, że zastosowanie bobiku, jako przedplonu skutkowało wyższymi wartościami S(f). Siew bezpośredni spowodował istotny wzrost stabilności gleby w porównaniu z orką. Zależność ta została jednak zidentyfikowana tylko w odniesieniu do głębszej warstwy gleby.
 
Morphological and agronomic features of potato cv. Gardena highly resistant to Phytophthora infestans (Mont.) de Bary depending on the nitrogen dose
Get PDFIn a 2-year field study, the impact of mineral nitrogen fertilization on the productivity of a new potato cultivar, promising due to the highest resistance to potato late blight among the registered ones, was compared to the proven, widely cultivated Denar cultivar. The study determined morphological features (size and weight of organs), physiological indicators (cover of soil by leaves – LAI. leaf greenness – SPAD) of potato plants during the growing season, yield and quality characteristics of tubers and optimal level of nitrogen fertilization. Tuber quality was assessed based on the share of tuber size and external defects in the yield structure. Optimal mineral nitrogen fertilization was determined based on the relationship between the increase in tuber yield and the increasing dose of this ingredient. The research took into account two factors: nitrogen dose (0, 50 kg‧ha–1, 100 kg‧ha–1, 150 kg‧ha–1) and cultivar (Gardena and Denar). The increase in the dose of mineral nitrogen fertilization to 150 kg‧ha–1 resulted in a significant increase in plant height, the weight of the root system, stems, leaves and the share of large tubers in the yield. It was shown that the Gardena cultivar was characterized by greater requirements for mineral nitrogen fertilization, low effectiveness of its use, a higher share of large tubers (diameter above 60 mm) and lower tuber yield than the Denar cultivar. In a year characterized by excess rainfall, plants produced a greater mass of the root system and the mass of the above-ground part, and in a year with an amount of rainfall close to optimal the final yield of tubers and the share of large tubers in the yield were higher.In a 2-year field study, the impact of mineral nitrogen fertilization on the productivity of a new potato cultivar, promising due to the highest resistance to potato late blight among the registered ones, was compared to the proven, widely cultivated Denar cultivar. The study determined morphological features (size and weight of organs), physiological indicators (cover of soil by leaves – LAI. leaf greenness – SPAD) of potato plants during the growing season, yield and quality characteristics of tubers and optimal level of nitrogen fertilization. Tuber quality was assessed based on the share of tuber size and external defects in the yield structure. Optimal mineral nitrogen fertilization was determined based on the relationship between the increase in tuber yield and the increasing dose of this ingredient. The research took into account two factors: nitrogen dose (0, 50 kg‧ha–1, 100 kg‧ha–1, 150 kg‧ha–1) and cultivar (Gardena and Denar). The increase in the dose of mineral nitrogen fertilization to 150 kg‧ha–1 resulted in a significant increase in plant height, the weight of the root system, stems, leaves and the share of large tubers in the yield. It was shown that the Gardena cultivar was characterized by greater requirements for mineral nitrogen fertilization, low effectiveness of its use, a higher share of large tubers (diameter above 60 mm) and lower tuber yield than the Denar cultivar. In a year characterized by excess rainfall, plants produced a greater mass of the root system and the mass of the above-ground part, and in a year with an amount of rainfall close to optimal the final yield of tubers and the share of large tubers in the yield were higher
Oddziaływanie gnojowicy i kondycjonerów glebowych na wybrane parametry wartości pokarmowej traw w uprawie polowej
Get PDFIn a two-year field experiment, the interaction of slurry with Humus Active and UGmax soil conditioners on selected quality characteristics of two species of fodder grass was assessed. The experiment used cattle slurry, UGmax and Humus Active soil conditioners, as well as mineral NPK. The test plants were the grasses Lolium multiflorum cultivar Dukat and Festulolium braunii (K Richt.) A. Camus cultivar Sulino. During two years of full use, three regrowths of cultivated grass were collected from each facility. The content of neutral-detergent fiber (NDF), acid-detergent fiber (ADF) and acid-detergent lignin (ADL) was determined in the plant material using near-infrared reflectance spectroscopy (NIRS). Based on the NDF and ADF content, the suitability of the feed raw material for animal production was determined, determining the relative nutritional value of RFV. The interaction of Humus Active and UGmax soil conditioners with slurry increased the NDF content in the biomass, but decreased the intake of dry matter DMI, in relation to biomass fertilized with slurry only.
Supplementing slurry with Humus Active and UGmax soil conditioners resulted in comparable NDF and ADF contents in the obtained biomass and its uptake (DMI) and digestibility (DDM) as supplementing slurry with NPK mineral fertilizers.W dwuletnim doświadczeniu polowym oceniono współdziałanie gnojowicy z kondycjonerami glebowymi Humus Active i UGmax na wybrane cechy jakościowe dwóch gatunków traw pastewnych. W eksperymencie zastosowano gnojowicę bydlęcą, kondycjonery glebowe UGmax i Humus Active, jak również nawożenie mineralne NPK. Roślinami testowymi były dwa gatunki traw Lolium multiflorum odmiany Dukat oraz Festulolium braunii (K Richt.) A. Camus odmiany Sulino. W ciągu dwóch lat pełnego użytkowania z każdego obiektu zebrano po trzy odrosty uprawianych traw. W materiale roślinnym oznaczono zawartość włókna neutralno-detergentowego (NDF), kwaśno-detergentowego (ADF) i lignin kwaśno-detergentowych (ADL) metodą spektroskopii odbiciowej w bliskiej podczerwieni (NIRS). Na podstawie zawartości NDF i DF określono przydatność surowca paszowego do produkcji zwierzęcej, wyznaczając względną wartość pokarmową RFV. Współdziałanie kondycjonerów glebowych Humus Active i UGmax z gnojowicą zwiększyło zawartości NDF w biomasie, zmniejszyło natomiast pobranie suchej masy DMI, w odniesieniu do biomasy nawożonej samą gnojowicą.
Gnojowica uzupełniona kondycjonerami glebowymi Humus Active i UGmax wpłynęła porównywalnie na zawartości NDF i ADF w otrzymanej biomasie oraz pobranie (DMI) i strawność (DDM) suchej masy, jak gnojowica zastosowana łącznie z nawozami mineralnymi NPK.
In vitro propagation, cold preservation and cryopreservation of Taxus baccata L., an endangered medicinal and ornamental shrub
Get PDFIn vitro propagation, cold preservation, and cryopreservation are three essential approaches to preserve the genetic resources of red-listed plants, including English yew (Taxus baccata L.). Different concentrations of plant growth regulators (PGRs) and different pre-treatments of cold preservation and cryopreservation are the prerequisites of these three approaches. Apical bud as explant and Murashige and Skoog (MS) as the culture medium for all three sections of the research, kinetin (Kin) and indole-3-butyric acid (IBA) as PGRs for the micropropagation section, and encapsulation-dehydration as pre-treatment for the sections of cold preservation and cryopreservation were used. The results of the micropropagation section indicated that the highest number of shoots (5.40 per explant) and roots (5.98 per explant) were obtained from the culture of the explants on the media containing 1 mg L–1 IBA together with 1 and 2 mg L–1 Kin, respectively. The results of the cold preservation section revealed that the highest percentage of survival of germplasms (100%) after storage in the refrigerator was observed in the apical buds pre-treated by dehydration of encapsulated explants with 0.75 M sucrose for two hours, followed by dehydration under a laminar airflow cabinet for two hours. The results of the cryopreservation section demonstrated that the highest percentage of survival of germplasms (100%) after storage in liquid nitrogen was obtained in the apical buds pre-treated by encapsulation-dehydration under a laminar airflow cabinet for two hours. At the acclimatization stage, 100% of the plantlets acclimatized suitably with ex vitro conditions.In vitro propagation, cold preservation, and cryopreservation are three essential approaches to preserve the genetic resources of red-listed plants, including English yew (Taxus baccata L.). Different concentrations of plant growth regulators (PGRs) and different pre-treatments of cold preservation and cryopreservation are the prerequisites of these three approaches. Apical bud as explant and Murashige and Skoog (MS) as the culture medium for all three sections of the research, kinetin (Kin) and indole-3-butyric acid (IBA) as PGRs for the micropropagation section, and encapsulation-dehydration as pre-treatment for the sections of cold preservation and cryopreservation were used. The results of the micropropagation section indicated that the highest number of shoots (5.40 per explant) and roots (5.98 per explant) were obtained from the culture of the explants on the media containing 1 mg L–1 IBA together with 1 and 2 mg L–1 Kin, respectively. The results of the cold preservation section revealed that the highest percentage of survival of germplasms (100%) after storage in the refrigerator was observed in the apical buds pre-treated by dehydration of encapsulated explants with 0.75 M sucrose for two hours, followed by dehydration under a laminar airflow cabinet for two hours. The results of the cryopreservation section demonstrated that the highest percentage of survival of germplasms (100%) after storage in liquid nitrogen was obtained in the apical buds pre-treated by encapsulation-dehydration under a laminar airflow cabinet for two hours. At the acclimatization stage, 100% of the plantlets acclimatized suitably with ex vitro conditions