Czasopisma Uniwersytetu Przyrodniczy w Lublinie
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Modelowanie zagospodarowania wsi w kierunku rozwoju usług turystyczno-rekreacyjnych i pożytków ekologicznych
Get PDFThe research theme was to develop a concept for modelling villages to increase their multi-functionality and identify appropriate planning tools. An attempt was made to systematise potential planning intervention in village spatial structures. The aim of the study was also to illustrate, on the example of a fragment of the village of Garbów in the municipality of Dwikozy, the possibility of creating a new profile for an existing village structure with a relatively high potential for tourism and recreation to increase its multi-functionality. Potentially, such activities may improve the economic basis for the functioning of rural settlement units and influence the protection and further development of natural resources. The concept of retrofitting rural settlement areas with extensive tourist and recreational elements and improving ecological parameters is a planning solution aimed at enhancing the natural, existing natural and cultural values of these areas. The research reviewed and analysed selected rural municipalities regarding their natural, landscape, and recreational attractiveness. The example of the municipality of Mielnik nad Bugiem was used, whose model of tourism and educational development can illustrate the good application of the presented ideas of exemplary character. As a result of the analysis of the existing resources of the municipality and the village of Garbów, new solutions, including spatial regulations towards tourist and pro-ecological functions, were proposed.Tematem badań było opracowanie koncepcji modelowania wsi w celu zwiększenia jej wielofunkcyjności oraz wskazanie stosownych narzędzi planistycznych. Podjęto próbę usystematyzowania potencjalnej interwencji planistycznej w strukturach przestrzennych wsi. Celem opracowania było także zilustrowanie na przykładzie fragmentu wsi Garbów w gminie Dwikozy możliwości kreowania nowego profilu istniejącej struktury wiejskiej o dość wysokim potencjaleturystyczno-rekreacyjnym, aby zwiększyć jej wielofunkcyjność. Potencjalnie działania takie mogą zmierzać do polepszenia podstaw ekonomicznych funkcjonowania wiejskich jednostek osadniczych, a także wpłynąć na ochronę i dalszy rozwój zasobów przyrodniczych. Koncepcja doposażenia terenów osadnictwa wiejskiego w ekstensywne elementy o charakterze turystyczno-rekreacyjnym, a także poprawa parametrów ekologicznych jest rozwiązaniem planistycznym mającym nacelu wzmacnianie naturalnych, istniejących walorów przyrodniczych i kulturowych tych terenów.W badaniach zastosowano metodę przeglądu i analizy wybranych gmin wiejskich w aspekcie atrakcyjnościprzyrodniczo-krajobrazowej i rekreacyjnej. Posłużono się przykładem gminy Mielnik nad Bugiem, której model zagospodarowania turystyczno-edukacyjnego może być ilustracją dobrego zastosowania prezentowanych idei o charakterze wzorcowym. W wyniku przeprowadzonych analiz zasobów istniejących gminy i wsi Garbów zaproponowano nowe rozwiązania obejmujące regulacje przestrzenne w kierunku funkcji turystycznych oraz proekologicznych
Wpływ zróżnicowanej rozstawy rzędów i ilości wysiewu na rozwój i plonowanie soi (Glycine max (L.) Merrill). Cz. II. Plony nasion, resztek pozbiorowych i ich skład chemiczny
Get PDFIn 2015–2017, in the proving grounds of the Institute of Agroecology and Plant Production of Wrocław University of Environmental and Life Sciences, field studies were carried out on the different row spacing and sowing amount on the yielding and chemical content of soybean. The test included the assessment of the impact of the varied spacing of rows (15 cm, 30 cm) and the number of sown seeds (50, 75, 90) per unit area. In both of test, the “split-plot” method was used, four repetitions, with two variable factors. The seed yield and post-harvest residues were strongly dependent on the course of weather in particular years of field experiments. The factor of differentiated row spacing (15 cm, 30 cm) did not determine the yields of seeds and harvest residues and the yields of crude fat and total protein per hectare. No significant effect of differentiated row spacing and the number of sown seeds per 1 m2 on the chemical composition of soybean seeds was noted, except for the content of crude fat.W latach 2015–2017 na polach doświadczalnych Instytutu Agroekologii i Produkcji Roślinnej Uniwersytetu Przyrodniczego we Wrocławiu przeprowadzone zostały badania dotyczące wpływu zróżnicowanej rozstawy rzędów i ilości wysiewu na plonowanie i skład chemiczny soi uprawnej. Badania założono jako dwuczynnikowe w układzie split-plot, w czterech powtórzeniach, z dwoma czynnikami zmiennymi: zróżnicowaniem rozstawy rzędów (15 cm, 30 cm) oraz zróżnicowanej liczby wysiewanych nasion (50, 75, 90 nasion na 1 m2). Wielkość i jakość zebranych plonów nasion i resztek pozbiorowych były silnie uzależnione od warunków pogodowych w poszczególnych latach prowadzenia doświadczeń polowych. Czynnik zróżnicowanej rozstawy rzędów (15 cm, 30 cm) nie determinował uzyskanych plonów nasion i resztek pozbiorowych oraz wydajności tłuszczu surowego i białka ogółem z hektara. Nie odnotowano istotnego wpływu zróżnicowanej rozstawy rzędów i liczby wysiewanych nasion na jednostce powierzchni na skład chemiczny nasion soi, z wyjątkiem zawartości tłuszczu surowego
Biodiversity assessment of segetal flora, earthworms and terrestrial invertebrates in various agricultural production systems and crops
Get PDFThe functioning of societies depends on a number of goods and services provided by the natural environment. Knowledge about the benefits that humans derive from it is an important issue in the era of current environmental and climate changes. Agricultural systems and management methods (e.g. tillage, weed and pest control, fertilization, field consolidation, crop specialization and monoculture) are important for biodiversity, the presence of which is of great importance for people and the environment. The aim of this study was to assess bioenvironmental indicators such as weed flora, earthworms and terrestrial invertebrates biomass, in selected crops in an organic, integrated and conventional farming systems in southern Poland.
The results showed the highest biodiversity weeds, earthworms, and terrestrial invertebrates in crops grown in the organic system in comparison to the conventional or sustainable ones, where chemical herbicides were applied. Species diversity of weeds was, on average, twice as high in the organic system (21 species) compared to the integrated and conventional systems (10–11 species). In the organic system, the highest number of weeds (average 71 pcs m–2) accompanied spring wheat and the lowest number of weeds was observed in legume-grass mixture in the first year of use (average 28 pcs m–2). The highest biomass of earthworms in the soil was estimated under winter wheat and legume-grass mixtures. This indicator was half as much in the soil under plants grown in integrated and conventional systems. Terrestrial invertebrates were also most abundant in crops grown in the organic system, indicating that this agricultural production system is conducive to maintaining high biodiversity in agroecosystems. For winter wheat cultivated in the conventional and integrated systems, the invertebrate richness index was 2.5–3 times lower than in the organic system.The functioning of societies depends on a number of goods and services provided by the natural environment. Knowledge about the benefits that humans derive from it is an important issue in the era of current environmental and climate changes. Agricultural systems and management methods (e.g. tillage, weed and pest control, fertilization, field consolidation, crop specialization and monoculture) are important for biodiversity, the presence of which is of great importance for people and the environment. The aim of this study was to assess bioenvironmental indicators such as weed flora, earthworms and terrestrial invertebrates biomass, in selected crops in an organic, integrated and conventional farming systems in southern Poland.
The results showed the highest biodiversity weeds, earthworms, and terrestrial invertebrates in crops grown in the organic system in comparison to the conventional or sustainable ones, where chemical herbicides were applied. Species diversity of weeds was, on average, twice as high in the organic system (21 species) compared to the integrated and conventional systems (10–11 species). In the organic system, the highest number of weeds (average 71 pcs m–2) accompanied spring wheat and the lowest number of weeds was observed in legume-grass mixture in the first year of use (average 28 pcs m–2). The highest biomass of earthworms in the soil was estimated under winter wheat and legume-grass mixtures. This indicator was half as much in the soil under plants grown in integrated and conventional systems. Terrestrial invertebrates were also most abundant in crops grown in the organic system, indicating that this agricultural production system is conducive to maintaining high biodiversity in agroecosystems. For winter wheat cultivated in the conventional and integrated systems, the invertebrate richness index was 2.5–3 times lower than in the organic system
Evaluation of Ca(NO3)2 and various container cell size effects on some growth attributes and nutrient content of tomato transplants
Get PDFOptimizing container cell size and nutrition is crucial for enhancing the quality of vegetable transplants. The current study evaluated the effect of different cell sizes and Ca(NO3)2 on some properties of tomato (Solanum lycopersicum L.) transplants. Experimental treatment included four levels (0, 50, 100, and 150 mg L–1) of Ca(NO3)2 and 5 different cell sizes of containers (1, 2, 3, 4, and 5) in a factorial experiment based on a completely randomized design (CRD) with three replications under greenhouse conditions. Ca(NO3)2 and larger cell size, increased height, stem diameter, fresh and dry weights of roots and shoots, and concentration of chlorophyll, protein, SPAD, carbohydrates, and macro/micronutrients. The results revealed that maximum shoot and root fresh and dry weight, photosynthesis pigments, N, P, K, Ca, and Fe concentrations were recorded at 150 mg L–1 × cell size 5. In comparison, the highest Zn and Mn concentrations were recorded at 100 mg L–1 × cell size 4 and 5. Our results demonstrated that applying Ca(NO3)2 and increasing the cell size of the containers improved the traits evaluated, so Ca(NO3)2 at 10 and 15 mg L–1 with cell size 5 can be recommended to transplant producers.Optimizing container cell size and nutrition is crucial for enhancing the quality of vegetable transplants. The current study evaluated the effect of different cell sizes and Ca(NO3)2 on some properties of tomato (Solanum lycopersicum L.) transplants. Experimental treatment included four levels (0, 50, 100, and 150 mg L–1) of Ca(NO3)2 and 5 different cell sizes of containers (1, 2, 3, 4, and 5) in a factorial experiment based on a completely randomized design (CRD) with three replications under greenhouse conditions. Ca(NO3)2 and larger cell size, increased height, stem diameter, fresh and dry weights of roots and shoots, and concentration of chlorophyll, protein, SPAD, carbohydrates, and macro/micronutrients. The results revealed that maximum shoot and root fresh and dry weight, photosynthesis pigments, N, P, K, Ca, and Fe concentrations were recorded at 150 mg L–1 × cell size 5. In comparison, the highest Zn and Mn concentrations were recorded at 100 mg L–1 × cell size 4 and 5. Our results demonstrated that applying Ca(NO3)2 and increasing the cell size of the containers improved the traits evaluated, so Ca(NO3)2 at 10 and 15 mg L–1 with cell size 5 can be recommended to transplant producers
Determination of biological activity of ethanol extracts of date-plum (Diospyros lotus) fruits and seeds growing in Turkey
Get PDFThis study was carried out to evaluate quality criteria such as chemical composition and antioxidant and antibacterial activity values in 80% ethanol extracts of date-plum (Diospyros lotus) fruits and seeds. In addition, the macro and microelement concentrations of fruits and seeds were also investigated. According to the data obtained, when fruit and seed were compared, it was determined that the seed (81.72%) contained more components than the fruit (79.4%), and the antioxidant activity of the seed was also higher. While the main component of the seed was “Methyl hydrogen disulfide” with 43.21%, the main component of the fruit was “5-Hydroxymethylfurfural” with 24.2%. As a result of antimicrobial activity tests, neither seeds nor fruits have antimicrobial activity. At the same time, the nutritional content values of the seeds and fruits of this plant were analyzed and evaluated. When the data obtained are evaluated in terms of both macro and micronutrients, it has been observed that the nutritional content values of the fruit (K (3.63%), P (0.68 %), Cu (24.31%), Zn (10.49 mg kg–1) and Mn (25.29 mg kg–1) for fruits) are higher than the seeds. In conclusion, the findings from the evaluation of Diospyros lotus fruit and seeds in this study highlight the richness in chemical composition and high antioxidant activity of the seeds, as well as the nutritional superiority of the fruit. Therefore, further research to better understand and harness the potential health benefits of this plant could contribute to a deeper understanding of this field.This study was carried out to evaluate quality criteria such as chemical composition and antioxidant and antibacterial activity values in 80% ethanol extracts of date-plum (Diospyros lotus) fruits and seeds. In addition, the macro and microelement concentrations of fruits and seeds were also investigated. According to the data obtained, when fruit and seed were compared, it was determined that the seed (81.72%) contained more components than the fruit (79.4%), and the antioxidant activity of the seed was also higher. While the main component of the seed was “Methyl hydrogen disulfide” with 43.21%, the main component of the fruit was “5-Hydroxymethylfurfural” with 24.2%. As a result of antimicrobial activity tests, neither seeds nor fruits have antimicrobial activity. At the same time, the nutritional content values of the seeds and fruits of this plant were analyzed and evaluated. When the data obtained are evaluated in terms of both macro and micronutrients, it has been observed that the nutritional content values of the fruit (K (3.63%), P (0.68 %), Cu (24.31%), Zn (10.49 mg kg–1) and Mn (25.29 mg kg–1) for fruits) are higher than the seeds. In conclusion, the findings from the evaluation of Diospyros lotus fruit and seeds in this study highlight the richness in chemical composition and high antioxidant activity of the seeds, as well as the nutritional superiority of the fruit. Therefore, further research to better understand and harness the potential health benefits of this plant could contribute to a deeper understanding of this field
The effect of zinc oxide nanoparticles on the growth and development of Stevia plants cultured in vitro
Get PDFStevia (Stevia rebaudiana Bertoni) is an essential herbal plant used as a sweetener. The demand for stevia is growing due to its low caloric and medicinal value, hence the need for a more thorough investigation of its nutritional and biological properties. Nanoparticles of metal oxides have been found to have broad applications in agriculture for the stimulation of plant growth and development. The study aimed to assess the effect of various zinc oxide nanoparticles (ZnONPs) concentrations on stevia plants’ quantitative and qualitative traits obtained in in vitro cultures. Micropropagation of two stevia varieties, Candy and Morita, was carried out using explants of shoot tips placed on MS medium supplemented with 1.0 mg dm–3 BA and 0.1 mg dm–3 IBA and with ZnONPs at concentrations of 0 (control), 10, 20, 30 and 40 mg dm–3. The obtained results indicated that high concentrations of ZnONPs stimulated the propagation of shoots. On the other hand, they negatively influenced shoot length, root number and length, and the fresh weight of the plantlets. The presence of zinc oxide nanoparticles in the medium increased the potassium, calcium, magnesium, and zinc content while decreasing the sodium and iron content in the regenerated stevia plantlets. The total phenolic content in the Candy variety was higher in the treatments with ZnONPs than in the control plants, while it was varied in the Morita variety. In both varieties, total antioxidant content measured by the ABTS method showed significantly higher in the treatments with 20–30 mg dm–3 ZnONPs than in the control. The content of chlorophyll a, chlorophyll b and chlorophyll a + b in the Morita variety was higher in the treatments with 10 and 20 mg dm–3 ZnONPs than in the control. On the other hand, high concentrations of ZnONPs negatively affected the content of carotenoids in both varieties. The study showed that stevia plants obtained in in vitro cultures on control media and media containing ZnONPs had a high content of valuable minerals, phytocompounds with antioxidant properties, and photosynthetic pigments.Stevia (Stevia rebaudiana Bertoni) is an essential herbal plant used as a sweetener. The demand for stevia is growing due to its low caloric and medicinal value, hence the need for a more thorough investigation of its nutritional and biological properties. Nanoparticles of metal oxides have been found to have broad applications in agriculture for the stimulation of plant growth and development. The study aimed to assess the effect of various zinc oxide nanoparticles (ZnONPs) concentrations on stevia plants’ quantitative and qualitative traits obtained in in vitro cultures. Micropropagation of two stevia varieties, Candy and Morita, was carried out using explants of shoot tips placed on MS medium supplemented with 1.0 mg dm–3 BA and 0.1 mg dm–3 IBA and with ZnONPs at concentrations of 0 (control), 10, 20, 30 and 40 mg dm–3. The obtained results indicated that high concentrations of ZnONPs stimulated the propagation of shoots. On the other hand, they negatively influenced shoot length, root number and length, and the fresh weight of the plantlets. The presence of zinc oxide nanoparticles in the medium increased the potassium, calcium, magnesium, and zinc content while decreasing the sodium and iron content in the regenerated stevia plantlets. The total phenolic content in the Candy variety was higher in the treatments with ZnONPs than in the control plants, while it was varied in the Morita variety. In both varieties, total antioxidant content measured by the ABTS method showed significantly higher in the treatments with 20–30 mg dm–3 ZnONPs than in the control. The content of chlorophyll a, chlorophyll b and chlorophyll a + b in the Morita variety was higher in the treatments with 10 and 20 mg dm–3 ZnONPs than in the control. On the other hand, high concentrations of ZnONPs negatively affected the content of carotenoids in both varieties. The study showed that stevia plants obtained in in vitro cultures on control media and media containing ZnONPs had a high content of valuable minerals, phytocompounds with antioxidant properties, and photosynthetic pigments
Influence of plant regulators on the micropropagation of Echinacea purpurea ‘Raspberry Truffle’
Get PDFA micropropagation protocol was conducted for Echinacea purpurea ‘Raspberry Truffle’ to determine the influence of medium amendment with a plant growth regulator (PGR). The efficacy of meta-Topolin (mT) and benzyladenine (BA) was evaluated during the proliferation stages. Additionally, indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) were assessed during the rooting stages. Multiple shoots were initiated and proliferated on a Murashige and Skoog (MS) medium and supplemented with 1 mL·L–1 of a Plant Preservative Mixture (PPM) and selected plant growth regulators with concentrations of 0.5, 1 and 2 mg·L–1 for shoot proliferation; and 0.05, 0.1, 0.5 and 1 mg·L–1, for rooting performance. The combination of mT and BA in the medium significantly enhanced shoot regeneration and elongation. Both, mT and BA resulted in 100% shoot regeneration. mT at a concentration of 0.5 mg·L–1 in the MS medium induced the maximum number of shoots, followed by 0.5 mg·L–1 BA. The supplementation of 0.05 mg·L–1 and 1 mg·L–1 IBA, and 0.1 NAA mg·L–1 resulted in a 100% root percentage with the highest number of roots found in the media amended with 1 mg·L–1 IBA and 0.1 mg·L–1 NAA.A micropropagation protocol was conducted for Echinacea purpurea ‘Raspberry Truffle’ to determine the influence of medium amendment with a plant growth regulator (PGR). The efficacy of meta-Topolin (mT) and benzyladenine (BA) was evaluated during the proliferation stages. Additionally, indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) were assessed during the rooting stages. Multiple shoots were initiated and proliferated on a Murashige and Skoog (MS) medium and supplemented with 1 mL·L–1 of a Plant Preservative Mixture (PPM) and selected plant growth regulators with concentrations of 0.5, 1 and 2 mg·L–1 for shoot proliferation; and 0.05, 0.1, 0.5 and 1 mg·L–1, for rooting performance. The combination of mT and BA in the medium significantly enhanced shoot regeneration and elongation. Both, mT and BA resulted in 100% shoot regeneration. mT at a concentration of 0.5 mg·L–1 in the MS medium induced the maximum number of shoots, followed by 0.5 mg·L–1 BA. The supplementation of 0.05 mg·L–1 and 1 mg·L–1 IBA, and 0.1 NAA mg·L–1 resulted in a 100% root percentage with the highest number of roots found in the media amended with 1 mg·L–1 IBA and 0.1 mg·L–1 NAA
Ocena znaczenia łęgów oraz innych zbiorowisk leśnych Poleskiego Parku Narodowego, stanowiących ekosystemy hydrogeniczne dla liczebności i występowania płazów jako alternatywnej bazy pokarmowej dla wydry (Lutra lutra)
Get PDFThe main aim of the research was to assess the importance of riparian forests and other forest communities in the Poleski National Park, which constitute hydrogenic ecosystems for the number and occurrence of amphibians as an alternative food base for otters, and to determine the role of otters in limiting the occurrence of invasive fish species. The research was conducted in the Polesie National Park at 9 research sites. The otter’s potential food base was analyzed based on the composition of its excrement. In the conditions of the Polesie National Park during the period under study, amphibians did not constitute an alternative food base for otters, which is probably related to the high availability of fish. Based on the analysis of feces, it can be concluded that otters in the Poleski National Park may, to some extent, control the number of invasive and alien fish species, including the catfish and the silver crucian carp.Zasadniczym celem badań była ocena znaczenia łęgów oraz innych zbiorowisk leśnych Poleskiego Parku Narodowego stanowiących ekosystemy hydrogeniczne dla liczebności i występowania płazów jako alternatywnej bazy pokarmowej dla wydry oraz określenie roli wydry w ograniczaniu występowania gatunków inwazyjnych ryb. Badania prowadzono na obszarze Pole-skiego Parku Narodowego na 9 stanowiskach badawczych. Potencjalną bazę pokarmową wydry analizowano na podstawie składu odchodów. W warunkach Poleskiego Parku Narodowego w badanym okresie płazy nie stanowiły alternatywnej bazy pokarmowej dla wydry, co prawdopodob-nie związane jest z dużą dostępnością ryb. Na podstawie analizy odchodów można stwierdzić, że wydry na obszarze Poleskiego Parku Narodowego mogą w pewnym stopniu kontrolować liczeb-ność gatunków inwazyjnych i obcych ryb, w tym sumika karłowatego oraz karasia srebrzystego
Opłacalność produkcji roślinnej na przykładzie indywidualnego gospodarstwa rolnego
Get PDFThis thesis discusses the issue of production profitability in the individual farm. The aim of the paper was to examine plant production profitability, based on a chosen individual agricultural holding that specializes in this type of production. Individual Farm Reports from PL FADN agricultural accounting system from years 2019–2021 were the basis of data for calculations and analysis. In the carried out analysis, the value and structure of the costs and their distribution where traced. Production profitability for different characteristics of the analyzed agricultural holding were calculated. The calculated cost-effectiveness indicators of the researched farm were increasing with each year. In 2021, the cost-effectiveness indicator calculated without subsidies, increased by over 65% compared to 2019, and if calculated with subsidies – increased by almost 75%. In the years considered, the highest cost-effectiveness indicators were recorded for winter rape and winter wheat and in 2021 for grain maize. On the basis of the calculations and analyses carried out, it can be concluded that the agricultural holding concerned acted effectively and rationally, income increased in subsequent years and there was a general improvement in the profitability of production.Praca przedstawia opłacalność produkcji w gospodarstwie indywidualnym. Celem badań była ocena opłacalności produkcji roślinnej na przykładzie wybranego indywidualnego gospodarstwa specjalizującego się w tej produkcji. Podstawą danych do kalkulacji i analiz były Raporty Indywidualne Gospodarstwa prowadzone w systemie rachunkowości rolniczej PL FADN w latach 2019–2021. W przeprowadzonej analizie prześledzono wartość i strukturę kosztów oraz ich podział. Policzono opłacalność produkcji poszczególnych działalności występujących w badanym gospodarstwie. Obliczone wskaźniki opłacalności dla badanego gospodarstwa osiągały z roku na rok wyższe wartości. W 2021 r. wskaźnik opłacalności liczony bez dopłat wzrósł o ponad 65 punktów procentowych w porównaniu do 2019 r. i o 74,5 punktu procentowego z uwzględnieniem dopłat. W rozpatrywanych latach najwyższe wskaźniki opłacalności odnotowano dla rzepaku ozimego i pszenicy ozimej oraz w 2021 r. dla kukurydzy na ziarno. Na podstawie przeprowadzonych kalkulacji i analiz można stwierdzić, że badane gospodarstwo prowadziło racjonalną działalność, w której dochody zwiększały się w kolejnych latach, co wpłynęło na ogólną poprawę opłacalności
Effect of preparations containing humic substances and pure humic acids on colony growth and spore germination of entomopathogenic fungi from the Beauveria genus
Get PDFThe experiment examined the effect of commercially available preparations containing humic substances in comparison with pure humic acids on the growth and germination of spores of entomopathogenic fungi (EPF) of the genus Beauveria in vitro. AmiAGRA, HumiAGRA, AlgoHUM (recommended field dose) and pure humic acids extracted from peat, brown coal and mushroom substrate were added to Sabouraud's culture substrate. Observation of the growth of colonies of the tested species of EPF was carried out every 5 days until day 20, measuring their diameter (mm). In the second stage of the experiment, the culture medium with the addition of preparations and pure humic acids was applied in a thin layer to the surface of glass slides and 0.1 ml of an aqueous solution with spores was introduced. Observation was carried out after 24 and 48 hours, and the results obtained were expressed as percentages of the control. The conducted research showed that on the 20th day of culture (on average), preparations containing humic substances had a stimulating effect, while pure acids slightly limited the growth of colonies of the tested isolates. The growth of the colony of the fungus B. basianna was most strongly stimulated by AmiAGRA, and B. brongniartii by HumiAGRA. The share of germinated spores of the tested isolates after 48 hours of contact with the substrate was higher than after 24 hours. It was found that more spores germinated on substrates with the addition of preparations containing humic substances than on pure humic acids.W doświadczeniu zbadano wpływ dostępnych na rynku preparatów zawierających substancje humusowe w porównaniu z czystymi kwasami humusowymi na wzrost i kiełkowanie zarodników grzybów entomopatogenicznych z rodzaju Beauveria w warunkach in vitro. Preparaty AmiAGRA, HumiAGRA, AlgoHUM (zalecana dawka polowa) oraz czyste kwasy humusowe, które zostały wyekstrahowane z torfu, węgla brunatnego oraz podłoża popieczarkowego dodano do podłoża hodowlanego Sabourauda. Obserwację wzrostu kolonii testowanych gatunków grzybów owadobójczych prowadzono co 5 dni, aż do dnia 20 mierząc ich średnicę (mm). W drugim etapie doświadczenia, podłoże hodowlane z dodatkiem preparatów i czystych kwasów humusowych nanoszono cienką warstwą na powierzchnię szkiełek podstawowych i wprowadzono 0,1 ml wodnego roztworu z zarodnikami. Obserwację prowadzono po 24 i 48 godzinach, a uzyskane wyniki wyrażono procentowo w stosunku do kontroli. Przeprowadzone badania wykazały, że w 20 dniu hodowli (średnio) preparaty zawierające substancje humusowe działały stymulująco, natomiast czyste kwasy nieznacznie ograniczały wzrost kolonii testowanych izolatów. Wzrost kolonii grzyba B. basianna najsilniej stymulował preparat AmiAGRA, a B. brongniartii HumiAGRA. Udział skiełkowanych zarodników testowanych izolatów, po 48 godzinach kontaktu z podłożem był większy niż po 24. Stwierdzono iż, więcej zarodników kiełkowało na podłożach z dodatkiem preparatów zawierających substancje humusowe, niż czyste kwasy humusowe.